Carbon nanodots with optical excitation active oxygen generating performance and preparation method thereof

A technology of carbon nanodots and active oxygen, which is applied in nanocarbon, nanotechnology, nanotechnology, etc., can solve the problems of complex preparation methods and high costs, and achieve good solubility

Active Publication Date: 2019-04-09
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The method of the present invention overcomes the disadvantages of complicated preparation methods and high cost of existing photosensitizers, and uses two simple r

Method used

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  • Carbon nanodots with optical excitation active oxygen generating performance and preparation method thereof
  • Carbon nanodots with optical excitation active oxygen generating performance and preparation method thereof
  • Carbon nanodots with optical excitation active oxygen generating performance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of carbon nanodots

[0018] Weigh 5 mg of dopamine solid and dissolve it in 5 mL of distilled water, add 5 mL of 3-aminopropyltriethoxysilane, 40 mL of distilled water and 5 mL of ammonia water (concentration 14.8 mol / L) to the solution in sequence, and place the above solution in a 100 mL round bottom In the flask, mix evenly, put in a strip-shaped magnetic stirrer, place the round-bottomed flask in a heating-type magnetic stirring water bath, and react in the dark at 60°C for 16 hours; The yellow liquid passes through a 0.22 µm microporous membrane, and the filtrate is dialyzed with a dialysis bag with a molecular weight cut-off of 500-1000 for 24 hours at room temperature and protected from light, and the ultrapure water dialysate is changed every 8 hours. After concentrating by rotary evaporation, freeze-dry for 24 hours to obtain powdery carbon nanodots.

[0019] The above powdered carbon nano-dots were prepared into a 0.05mg / mL solution with distilled...

Embodiment 2

[0023] Figure 5 A, b, and c in the above show that the detected absorbance value at 655 nm of the carbon nanodot solution, TMB solution, and carbon nanodot / TMB mixed solution that has not been irradiated with a wavelength of 395 nm is basically zero, indicating that without care, the above The solution did not change color due to oxidation. Figure 5 The d and e in the figure show that after irradiating with a wavelength of 395 nm for 1 hour, the detected absorbance values ​​of the carbon nanodot solution and TMB solution at 655 nm are basically the same as those of the non-irradiated group, indicating that the simple carbon nanodot solution and TMB solution after light irradiation No discoloration due to oxidation. but by Figure 5 In f, it can be seen that when the carbon nanodot solution-TMB mixed solution is irradiated with a 395 nm wavelength light source for a period of time, the color of the mixed solution changes significantly, from colorless to blue, and the absorp...

Embodiment 3

[0025] Directly scan the ESR spectrum of the carbon nanodot solution, Image 6 (a) shows g = 1.9879 for the carbon nanodot solution.

[0026] Use a capillary to take the mixed solution of carbon nanodot solution and TEMP, put it into the sample tube, and measure its ESR signal after irradiating with 365nm light for 10min. Image 6 (b) shows the ESR signal of the singlet oxygen-TEMP binding product TEMPO. The results show that the carbon nanodots can generate singlet oxygen after light irradiation.

[0027] Use a capillary to take the mixed solution of carbon nanodot solution and DMPO, put it into a sample tube, and measure its ESR signal after irradiating with 365nm light for 10min. Image 6 (c) in and Image 6(d) shows that the aqueous solution of carbon nanodots can detect the characteristic peak of the adduct DMPO-OH generated after the signal DMPO captures the hydroxyl radical after being illuminated (the quartet with an intensity ratio of 1:2:2:1 ), and the characteri...

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Abstract

The invention discloses carbon nanodots with optical excitation active oxygen generating performance and a preparation method thereof. The preparation method of carbon nanodots with optical excitationactive oxygen generating performance belongs to the technical field of preparation of carbon nano materaisl (carbon dots). The preparation method comprises the following steps: preparing a carbondotaqueous solution at 60 DEG C by means of a mixed solution of dopamine and 3-aminopropyl triethoxy silane; and carrying out dialysis and purification to obtain silicon-doped carbon nanodots. The carbonnanodots with optical excitation active oxygen generating performance uniform and high in fluorescence intensity can be synthesized in a mesotherm condition, and have an important application prospect in bio-imaging and sensing. The synthetic method is simple and low in cost and can be finished in a common lab.

Description

technical field [0001] The invention belongs to the technical field of carbon nanomaterial preparation, and in particular relates to a method for preparing carbon nanodots with light-excited active oxygen generation performance by using dopamine and 3-aminopropyltriethoxysilane by heating in a water bath. Background technique [0002] Cancer is one of the major diseases that endanger human health. In recent decades, people have been devoting themselves to the early diagnosis and treatment of tumors. On the basis of traditional surgery, radiotherapy and chemotherapy, many new anti-tumor therapies have been developed, such as gene therapy and photodynamic therapy. Photodynamic therapy is a new type of cancer treatment developed in recent years. After it injects non-toxic photosensitizer into tumor tissue, it excites the photosensitizer with light of specific wavelength, and the photosensitizer forms reactive oxygen species that can damage cells, thereby causing damage inside...

Claims

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Application Information

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IPC IPC(8): C01B32/15C09K11/65G01N21/64B82Y20/00
CPCG01N21/6428C09K11/65B82Y20/00C01B32/15
Inventor 林珍林新华罗舒姚文松陈伟
Owner FUJIAN MEDICAL UNIV
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