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Detection kit for glycocholic acid for eliminating chyle interference

A technology for detecting kits and glycocholic acid, which is applied in measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of increasing the workload of operators, increasing operating costs, and timely and effective clinical testing, so as to improve the effectiveness Effects of binding rate, short detection time, improved stability and sensitivity

Inactive Publication Date: 2016-11-16
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Considering that the method is practicable when the sample is small, but when large-scale population census, health examination screening and clinical patient testing, a large number of turbid samples such as chyle and hemolysis are often encountered. At this time, through the existing routine The detection kit obviously cannot solve such problems in a timely and effective manner. If these samples are processed individually one by one by purchasing sample processing agents, it will undoubtedly greatly increase the workload of operators, which not only increases operating costs, but also paves the way for timely clinical testing. Effectively brings up the puzzle

Method used

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  • Detection kit for glycocholic acid for eliminating chyle interference
  • Detection kit for glycocholic acid for eliminating chyle interference
  • Detection kit for glycocholic acid for eliminating chyle interference

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A detection kit for glycocholic acid that removes chyle interference includes components and concentrations as follows:

[0035] Reagent R1: pH=7

[0036]

[0037]

[0038] Reagent R2: pH=6.5

[0039]

[0040] Calibrator:

[0041]

[0042] According to the required concentration of glycocholic acid reference calibrator, add the corresponding pure glycocholic acid to the above buffer respectively to obtain glycocholic acid calibrator with different concentrations: 0mg / L, 2.5mg / L, 5.0mg / L, 10.0mg / L, 20.0mg / L, 40.0mg / L, 80mg / L.

[0043] The preparation method of the latex particles of the goat anti-human glycocholic acid monoclonal antibody comprises the following steps:

[0044] (1) Put the carboxylated polystyrene latex into PBS buffer solution with pH=5.0-6.5 for activation.

[0045] (2) Put the anti-human glycocholic acid monoclonal antibody into PBS buffer solution with pH=3.0-4.0 for acidification.

[0046] (3) The solution obtained in step (2) is r...

Embodiment 2

[0050] A detection kit for glycocholic acid that removes chyle interference includes components and concentrations as follows:

[0051] Reagent R1: pH=8

[0052]

[0053] Reagent R2: pH6.5

[0054]

[0055] Calibrator:

[0056]

[0057] According to the required concentration of glycocholic acid reference calibrator, add the corresponding pure glycocholic acid to the above buffer respectively to obtain glycocholic acid calibrator with different concentrations: 0mg / L, 2.5mg / L, 5.0mg / L, 10.0mg / L, 20.0mg / L, 40.0mg / L, 80mg / L.

[0058] The preparation method of the latex particles of the rabbit anti-human glycocholic acid monoclonal antibody comprises the following steps.

[0059] (1) Put the carboxylated polystyrene latex into MES buffer solution with pH=5.0-6.5 for activation.

[0060] (2) Put the anti-human glycocholic acid monoclonal antibody into MES buffer solution with pH=3.0-4.0 for acidification.

[0061] (3) Wash the solution obtained in step (2) repeatedly...

Embodiment 3

[0065] 1. The detection kit for glycocholic acid that eliminates chyle interference includes the following components and concentrations:

[0066] Reagent R1: pH=6.5

[0067]

[0068] Reagent R2: pH=6.5

[0069]

[0070]

[0071] Calibrator:

[0072]

[0073] According to the required concentration of glycocholic acid reference calibrator, add the corresponding pure glycocholic acid to the above buffer respectively to obtain glycocholic acid calibrator with different concentrations: 0mg / L, 2.5mg / L, 5.0mg / L, 10.0mg / L, 20.0mg / L, 40.0mg / L, 80mg / L.

[0074] The preparation method of the latex particles of the mouse anti-human glycocholic acid monoclonal antibody comprises the following steps:

[0075] (1) Put the carboxylated polystyrene latex into MES buffer solution with pH=5.0-6.5 for activation.

[0076] (2) Put the anti-human glycocholic acid monoclonal antibody into MES buffer solution with pH=3.0-4.0 for acidification.

[0077] (3) Wash the solution obtain...

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Abstract

The invention discloses a detection kit for glycocholic acid for eliminating chyle interference. The detection kit comprises a reagent R1, a reagent R2 and a calibrator. The reagent R1 contains buffer solution a, chyle elimination agent, stabilizer a, surfactant a, reaction promoter a and preservative a; the reagent R2 contains buffer solution b, latex particles of antihuman glycocholic acid monoclonal antibody, stabilizer b, surfactant b, preservative b and reaction promoter b; the calibrator contains buffer solution c, glycocholic acid standard, stabilizer c, surfactant c, reaction promoter c and preservative c. The detection kit is short in detection time, strong in anti-interference capacity, good in stability and repeatability and high in accuracy and detection sensitivity.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection kit for glycocholic acid that eliminates chyle interference. Background technique [0002] Cholyglycine (CG) is one of the conjugated cholic acids formed by the combination of bile acid and glycine. In hepatocytes, cholesterol is transformed into primary bile acids through an extremely complex enzymatic reaction. Among them are cholic acid (CA) and chenodeoxycholic acid (CD-CA). There are three hydroxyl groups (C3, C7, C12) on the steroid core of cholic acid, and the hydroxyl group at the end of the side chain is combined with glycine through a peptide bond. The normal metabolic pathway of CG is the entero-hepatic circulation. CG is synthesized by liver cells, discharged into the gallbladder through the capillaries and bile ducts, and enters the duodenum along with bile to help digest food. 95% of bile acid is reabsorbed in the terminal ileum, and then returned ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/577G01N33/531
CPCG01N21/31G01N33/531G01N33/577
Inventor 杨致亭魏红刘雪梅陈勇高爱菊徐洋
Owner 山东康华生物医疗科技股份有限公司
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