6results about How to "Reduce non-specific binding" patented technology

The application relates to the field of molecular biology and discloses a LAMP primer group and a kit for detecting influenza A virus H1N1. The primer group comprises an outer primer pair, an inner primer pair and a loop primer pair; the outer primer pair comprises a forward outer primer with a nucleotide sequence as shown in SEQ ID NO. 1 and a reverse outer primer with a nucleotide sequence as shown in SEQ ID NO. 2; the inner primer pair comprises a forward inner primer with a nucleotide sequence as shown in SEQ ID NO. 3 and a reverse inner primer with a nucleotide sequence as shown in SEQ ID NO. 4; and the loop primer pair comprises a forward loop primer with a nucleotide sequence as shown in SEQ ID NO. 5 and a reverse loop primer with a nucleotide sequence as shown in SEQ ID NO. 6. The primer group has good thermal stability, is suitable for 70 DEG C high-temperature LAMP amplification, reduces non-specific binding, and has good sensitivity and specificity.

This invention discloses a preparation process for a targeted drug delivery system based on the synergistic effect of Lamp2b and IMTP, comprising the following steps: constructing cells overexpressing the Lamp2b-IMTP fusion protein, culturing cells overexpressing the Lamp2b-IMTP fusion protein, thereby obtaining the targeted drug delivery system. The Lamp2b / IMTP synergistic targeted drug delivery system obtained by this invention allows for the specific accumulation of drugs in target cells, such as tumor cells, achieving precise drug delivery. The fundamental localization role of Lamp2b: Lamp2b is a naturally occurring transmembrane protein on the surface of exosomes, responsible for mediating the "initial recognition" between exosomes and recipient cells, ensuring that the delivery system, with exosomes as the core carrier, can be effectively recognized and bound by cells, avoiding rapid clearance by the body as a "foreign substance."

This application relates to the technical field of bioassay, specifically disclosing a combined detection kit for pepsinogen I and pepsinogen II and its application. The disclosed combined detection kit for pepsinogen I and pepsinogen II includes: a sample pad, a conjugate pad, a coating membrane, and an absorbent pad; the coating membrane is an NC membrane coated with antibody; the conjugate pad is prepared by pretreating and drying a fiber membrane, followed by spraying time-resolved fluorescent microspheres labeled with antibody; the pretreating solution is a solution of pH 8.0-8.4 + 8-12mM BS + 0.8-1.2% BSA + 0.8-1.2% PEG4000 + 0.02-0.03% surfactant + 4-6% sucrose. Using the detection kit and method provided in this application for the combined detection of pepsinogen I / pepsinogen II exhibits high sensitivity, accuracy, and repeatability.

The present application relates to a kind of fluorescent enhancement composition and fluorescent enhancement liquid for tyramide signal amplification system.The fluorescent enhancement composition of the present application includes enhancer and cluster agent;The molar ratio of the enhancer and cluster agent is (0.1-1) mol:(1-10) mmol;The enhancer is one or more than two combinations of 4,4-bis (5-methyl-2-benzoxazolyl) stilbene, 2,5-bis (5-tert-butyl-2-benzoxazolyl) thiofene, 4,4'-bis (2-sulfonic acid sodium phenylvinyl) biphenyl and 1,2-bis (5-methyl-benzoxazolyl) -ethylene;The cluster agent is one or more than two combinations of alginic acid, povidone, methyl vinyl ether-maleic anhydride copolymer and carrageenan.The fluorescent enhancement liquid with the above-mentioned fluorescent enhancement composition for tyramide signal amplification system as main component can enhance the effect of fluorescent signal amplification, reduce non-specific coloring.

The invention provides EB (Epstein-Barr) virus nucleic acid detection primers, a probe and a kit, according to a target sequence of an EB virus gene, the sequences of the primers and the probe in an asymmetric primer ERA detection system are designed and screened, and the ratio of upstream and downstream primers and an amplification reaction system are optimized, so that the EB virus nucleic acid sequence can be specifically identified, and the EB virus nucleic acid detection kit has the advantages of high specificity and high sensitivity. Non-specific binding with other irrelevant nucleic acids is reduced, so that the detection specificity is improved; meanwhile, efficient amplification of EB virus nucleic acid is realized, and the EB virus nucleic acid can be accurately detected even under the condition that the content of the virus nucleic acid is extremely low, so that the detection sensitivity and the amplification efficiency are improved.

This invention provides a chemiluminescence detection reagent, composition, kit, and method. The chemiluminescence detection reagent of this invention includes a first conjugate capable of specifically binding to a target molecule, the first conjugate being linked to a label, the label having at least two hydrophilic arms, each arm ending in a specific pairing agent. The reagent, composition, kit, and method of this invention exhibit high anti-interference properties and good sensitivity.