Binding solution, kit and method for purifying nucleic acid by magnetic bead method as well as application

Abstract

The invention provides a binding solution, kit and method for purifying nucleic acid by magnetic bead method as well as application. The binding solution comprises a citrate buffer solution, EDTA, sodium acetate, lithium chloride and isopropyl alcohol but does not comprise guanidine salt. The binding solution contains no guanidine salt, can coexist with magnetic beads for a long time and can fullypromote precipitation of nucleic acid and binding with magnetic beads, the low isopropyl alcohol content contributes to increase of the nucleic acid purity, and nonspecific binding of impurities withmagnetic beads is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a binding liquid, a kit, a method and an application for magnetic bead nucleic acid purification. Background technique [0002] Magnetic bead method nucleic acid extraction reagent is a new technology product combining biochemistry and nanomaterial science. The chemically modified superparamagnetic nano magnetic beads can specifically bind to nucleic acid molecules in a specific ion reaction system. Using this principle, it can be obtained from various Isolate DNA from various animal tissues, cells, and microorganisms, and apply it in various downstream research and detection fields. [0003] The magnetic bead nucleic acid extraction process is divided into four steps: lysis-binding-washing-elution. Among them, the lysis requires the use of lysate and auxiliary additives such as proteinase K, dithiothreitol (DTT), etc., the combination needs to add magnetic beads to the specific ion r...

AI Technical Summary

Problems solved by technology

At present, most magnetic bead extraction kits on the market are selective for samples, and the components in the lysate are relatively simple. Most of them are configured according to traditional nucleic acid extraction methods. When encountering complex and diverse samples, different kits are often required. Nucleic acid extraction increases the difficulty of the operator's work; secondly, the binding step involves the mixing of nucleic acid adsorbent and magnetic beads. Most of the binding solutions on the market contain guanidinium salts. Although guanidinium salts can promote the precipitation of nucleic acids and the interaction with magnetic beads However, most of the magnetic beads cannot be stored with guanidinium salt for a long time. Therefore, during the production process, the production personnel need to store the magnetic beads and the binding solution separately. It is especially troublesome for the reagent production of automated equipment, and the magnetic beads and the binding solution need to be separated. Packing in different consumables increases the workload of production personnel; again, the best storage method for nucleic acid is -80°C or liquid nitrogen cryogenic storage, but most scientific research units or companies choose cheap TE buffer or ultrapure Water, stored at -20°C, but still cannot avoid nucleic acid degradation over time

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