Paper-chromatography chemiluminescent detection method

A technique of chromatography chemistry and luminescence detection, applied in the field of immunoassay

Inactive Publication Date: 2017-12-08
BEIJING KANGHUAYUAN SCI TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High sensitivity, rapidity, miniaturization, full quantification, and automation are the current development trends of

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Embodiment 1

[0079] Embodiment 1. Preparation of paper chromatography chemiluminescence detection combination kit of the present invention:

[0080] Such as figure 1As shown, the paper chromatography chemiluminescence detection combination kit of the present invention includes, analyte-specific binder 1, microparticle 2, solid-phase membrane 3, chemiluminescence enzyme or chemiluminescence substance 4, centrifugal device 5, luminescence detector 6, Chemiluminescent enzyme reaction substrate or starting luminescent reagent 7, also includes the second analyte-specific binding substance or intermediate substance B 8, label adsorption membrane pad 9, water-absorbing membrane pad 10, and supporting film 11. The specific binding substance 1 of the analyte and the chemiluminescent enzyme or chemiluminescent substance 4 are combined with the microparticle 2 and printed on the label adsorption film pad 8 . The solid-phase membrane 3 is printed with a second analyte-specific binder or intermediate ...

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Abstract

The invention discloses a combined chemiluminescent detection kit and a detection method. The combined chemiluminescent detection kit comprises a specific conjugate of a to-be-detected substance, a microparticle, a solid-phase film, a chemiluminescent reagent, a centrifugal device and a luminescent detector, wherein the chemiluminescent reagent comprises at least one selected from a group consisting of a chemiluminescent enzyme, a chemiluminescent substance, a chemiluminescent enzyme reaction substrate and a starting luminescent reagent; the microparticle is a particle capable of specifically binding to a protein and/or the chemiluminescent reagent in a direct binding and/or chemically crosslinking way and maintaining stable; the solid-phase membrane is a membranous substance having the characteristic of non-specific binding to a protein; the centrifugal device is a structure capable of centrifugally driving chromatographic flowing of a liquid phase on the solid-phase membrane; and the specific conjugate of the to-be-detected substance is at least one selected from a group consisting of antigen, antibody, avidin, biotin and analogue thereof with specific binding ability. The combined chemiluminescent detection kit and the detection method in the invention have the characteristics of high sensitivity and short detection time, and have good application prospects.

Description

technical field [0001] The invention relates to a paper chromatography chemiluminescence detection method, which belongs to the technical field of immune detection. Background technique [0002] Immunological detection technology is an experimental method designed by applying the principles of immunology to determine antigens, antibodies, immune cells and chemical components. Samples for analytical, food and industrial analysis. Commonly used immunoturbidity technology, solid-phase enzyme immunoassay technology, chemiluminescent detection technology, immunofluorescence labeling technology, flow cytometry, colloidal gold technology, etc. Immunoturbidimetry, also known as immunoturbidimetry, is the specific combination of soluble antigens and antibodies in the liquid phase to produce complexes of a certain size, which form light refraction or absorption, and measure the transmitted or scattered light after refraction or absorption. As a calculation unit, it is used for quant...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543G01N21/76
CPCG01N21/763G01N33/54313G01N33/558
Inventor 刘凤鸣
Owner BEIJING KANGHUAYUAN SCI TECH CO LTD
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