Paper-chromatography chemiluminescent detection method

Abstract

The invention discloses a combined chemiluminescent detection kit and a detection method. The combined chemiluminescent detection kit comprises a specific conjugate of a to-be-detected substance, a microparticle, a solid-phase film, a chemiluminescent reagent, a centrifugal device and a luminescent detector, wherein the chemiluminescent reagent comprises at least one selected from a group consisting of a chemiluminescent enzyme, a chemiluminescent substance, a chemiluminescent enzyme reaction substrate and a starting luminescent reagent; the microparticle is a particle capable of specifically binding to a protein and / or the chemiluminescent reagent in a direct binding and / or chemically crosslinking way and maintaining stable; the solid-phase membrane is a membranous substance having the characteristic of non-specific binding to a protein; the centrifugal device is a structure capable of centrifugally driving chromatographic flowing of a liquid phase on the solid-phase membrane; and the specific conjugate of the to-be-detected substance is at least one selected from a group consisting of antigen, antibody, avidin, biotin and analogue thereof with specific binding ability. The combined chemiluminescent detection kit and the detection method in the invention have the characteristics of high sensitivity and short detection time, and have good application prospects.

Description

technical field [0001] The invention relates to a paper chromatography chemiluminescence detection method, which belongs to the technical field of immune detection. Background technique [0002] Immunological detection technology is an experimental method designed by applying the principles of immunology to determine antigens, antibodies, immune cells and chemical components. Samples for analytical, food and industrial analysis. Commonly used immunoturbidity technology, solid-phase enzyme immunoassay technology, chemiluminescent detection technology, immunofluorescence labeling technology, flow cytometry, colloidal gold technology, etc. Immunoturbidimetry, also known as immunoturbidimetry, is the specific combination of soluble antigens and antibodies in the liquid phase to produce complexes of a certain size, which form light refraction or absorption, and measure the transmitted or scattered light after refraction or absorption. As a calculation unit, it is used for quant...

AI Technical Summary

Problems solved by technology

High sensitivity, rapidity, miniaturization, full quantification, and automation are the current development trends of clinical immunoassay technology products, but none of the existing ones can achieve the above functions at the same time

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