Centrifugal separation detection method

A detection method and centrifugal separation technology, which are applied in the field of immunoassay, can solve the problems that cannot be realized at the same time, and achieve the effect of automatic operation, fast detection and improved detection accuracy

Inactive Publication Date: 2019-02-26
BEIJING KANGHUAYUAN SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] High sensitivity, rapidity, miniaturization, full quantification, and automation are the current development trends of clinical immunoassay technology products, but none of the existing ones can achieve the above functions at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, the comparison of the detection accuracy of the present invention and existing detection technology

[0040] 1. Experimental materials

[0041] Anti-human myoglobin polyclonal antibody (U.S. Genagates, catalog number GP301042), anti-human myoglobin monoclonal antibody (U.S. Genagates, catalog number GP300616), spectrophotometer (Shanghai Qinghua Technology Instrument Co., Ltd., 752 UV-visible spectrophotometer), human myoglobin (Sigma-Aldrich product, catalog number is F3879-1G), BioFlow film printing instrument (USA IMAGENE company), Index strip cutter (USA A-point company), DBF -900 sealing machine (Wenzhou Jiangnan Packaging Factory), ACBO dehumidifier (Jiangsu Wuxi Aobo Dehumidifier Company), desktop centrifuge (US Eppendoff Company), bovine serum albumin (BSA for short, SIGMA product, item number: B8894), nitric acid Cellulose membrane (AE99, provided by Genagates, USA), polyester cellulose membrane (Reemay 2033, product of Alstrom Corporation, USA),...

Embodiment 2

[0054] Embodiment 2, the comparison of the present invention and the minimum detection amount of existing detection technology

[0055] 1. Experimental materials

[0056] Same as Example 1

[0057] 2. Experimental method

[0058] Same as Example 1

[0059] 3. Experimental results

[0060] The present invention uses colloidal gold as the assay result of indicator, analysis adopts the requirement that correlation coefficient r value commonly used in the development of related products is greater than 0.98, and the data is statistically processed, and the minimum value when the r value is greater than 0.98 is defined as the minimum detection amount. As a result, under the same experimental conditions, the pre-stand time is 1-15 minutes, the minimum detection amount of the prior art is 25 or > 25ng / ml, and the minimum detection amount of the present invention is 3.125ng / ml, the detection sensitivity Significantly higher than the prior art, indicating that the technology of the...

Embodiment 3

[0063] Embodiment 3, the influence of the present invention on detection specificity

[0064] 1. Experimental materials

[0065] Same as Example 1

[0066] 2. Experimental method

[0067] Same as Example 1

[0068] The samples used for specific detection are A: 50ng / ml myoglobin, B: 10ng / ml troponin I, C: 30ng / ml creatine kinase isoenzyme, D: 80mg / ml human serum albumin, E: 20mg / ml cholesterol.

[0069] 3. Experimental results

[0070] The present invention uses colloidal gold as an indicator to detect the above-mentioned specificity detection sample, and the experimental results are as shown in table 3. The repeated detection average values ​​of the myoglobin sample by the prior art and the technology of the present invention are respectively 50.3 and 51.0 ng / ml, and others are not The detection values ​​of the samples containing myoglobin were below the detection sensitivity lower limit of the detection method, all were negative, and there was no obvious color reaction...

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Abstract

The invention discloses a centrifugal separation detection method. The method comprises the steps of: a liquid phase is driven by a centrifugal device to flow through a solid phase membrane, wherein the liquid phase comprises a to-be-tested sample containing a to-be-tested substance and a detection phase, the detection phase is a substance containing a detection indicator capable of directly or indirectly specifically binding to the to-be-tested substance; when the liquid phase flows through the solid phase membrane, the liquid phase is combined with to-be-tested substance specific conjugate coating the solid phase membrane to form a detection indicator-to-be-tested substance-to-be-tested substance specific conjugate complex, the detection indicator-to-be-tested substance-to-be-tested substance specific conjugate complex is captured and immobilized on the solid phase membrane, and a detector detects the amount of the detection indicator indirectly fixed to the solid phase membrane to detect the amount of the to-be-tested substance. The centrifugal separation detection method has the characteristics of high sensitivity and short detection time.

Description

[0001] This application is a divisional application with the application number 201610143694.3, the application date is March 14, 2016, and the invention name is "a centrifugal separation detection method" technical field [0002] The invention relates to a centrifugal separation detection method, which belongs to the technical field of immune detection. Background technique [0003] Immunological detection technology is an experimental method designed by applying the principles of immunology to determine antigens, antibodies, immune cells and chemical components. Samples for analytical, food and industrial analysis. Commonly used immunoturbidity technology, solid-phase enzyme immunoassay technology, chemiluminescent detection technology, immunofluorescence labeling technology, flow cytometry, colloidal gold technology, etc. [0004] Immunoturbidimetry, also known as immunoturbidimetry, is the specific combination of soluble antigens and antibodies in the liquid phase to pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 刘凤鸣
Owner BEIJING KANGHUAYUAN SCI TECH CO LTD
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