Centrifugal chromatography colloidal gold immunodetection technology and application thereof

Abstract

The invention discloses a centrifugal chromatography colloidal gold immunodetection set. The detection set is characterized by containing a to-be-detected substance specific binder, colloidal gold particles, a solid phase membrane, a centrifugal device and a colloidal gold detector, wherein the colloidal gold particles are prepared from a gold solution and can be non-specifically bond with proteindirectly and kept stable; the solid phase membrane is a membrane substance with the characteristic of nonspecific binding with protein; the centrifugal device is a structure capable of centrifugallydriving a liquid phase to realize chromatographic flow on the solid phase membrane; the to-be-detected substance specific binder is at least one of an antigen, an antibody, avidin, biotin and analogues thereof with specific binding capacity; the colloidal gold detector performs quantitative or semi-quantitative detection through colorimetric analysis of the color of colloidal gold. The set has thecharacteristics of high sensitivity, good stability and short detection time and has bright application prospects.

Description

technical field [0001] The invention relates to a centrifugal chromatography colloidal gold immunodetection technology and application thereof, belonging to the technical field of immunodetection. Background technique [0002] Immunological detection technology is an experimental method designed by applying the principles of immunology to determine antigens, antibodies, immune cells and chemical components. Samples for analytical, food and industrial analysis. Commonly used immunoturbidity technology, solid-phase enzyme immunoassay technology, chemiluminescent detection technology, immunofluorescence labeling technology, flow cytometry, colloidal gold technology, etc. Immunoturbidimetry, also known as immunoturbidimetry, is the specific combination of soluble antigens and antibodies in the liquid phase to produce complexes of a certain size, which form light refraction or absorption, and measure the transmitted or scattered light after refraction or absorption. As a calcul...

AI Technical Summary

Problems solved by technology

High sensitivity, rapidity, miniaturization, full quantification, and automation are the current development trends of clinical immunoassay technology products, but none of the existing ones can achieve the above functions at the same time

The quantitative detection technology of colloidal gold immunological paper chromatography obviously affects the clinical application effect of the technology due to the instability of its reaction color intensity changing with time

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