Centrifugation chromatography fluoroimmunoassay detection method and applications thereof

Abstract

The invention discloses a fluorescence detection kit, and a detection method. The fluorescence detection kit comprises a substance capable of realizing specific binding with a substance to be detected, particles, a solid phase membrane, a fluorescent substance, a centrifugation device, and a fluorescence detector; wherein the fluorescent substance comprises at least one selected from an organic fluorescent dye and a rare earth element fluorescent dye; the particles are capable of realizing nonspecific binding with protein and / or the fluorescent substance directly and / or via chemical crosslinking, and maintaining stability; the solid state membrane is a membrane substance capable of realizing nonspecific binding with protein; the centrifugation device is capable of realizing centrifugationdriving of chromatography and flowing of a liquid phase on the solid phase membrane; and the substance capable of realizing specific binding with the substance to be detected is at least one selectedfrom an antigen, an antibody, an avidin, a biotin, and an analog with specific binding capacity. The detection method is high in sensitivity and stability, short in detection time, and promising in application prospect.

Description

technical field [0001] The invention relates to a centrifugal chromatography fluorescence immunoassay technology and application thereof, belonging to the technical field of immunoassay. Background technique [0002] Immunological detection technology is an experimental method designed by applying the principles of immunology to determine antigens, antibodies, immune cells and chemical components. Samples for analytical, food and industrial analysis. Commonly used immunoturbidity technology, solid-phase enzyme immunoassay technology, chemiluminescent detection technology, immunofluorescence labeling technology, flow cytometry, colloidal gold technology, etc. Immunoturbidimetry, also known as immunoturbidimetry, is the specific combination of soluble antigens and antibodies in the liquid phase to produce complexes of a certain size, which form light refraction or absorption, and measure the transmitted or scattered light after refraction or absorption. As a calculation unit...

AI Technical Summary

Problems solved by technology

High sensitivity, rapidity, miniaturization, full quantification, and automation are the current development trends of clinical immunoassay technology products, but none of the existing ones can achieve the above functions at the same time

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