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Heart-type fatty acid binding protein detection device with starting control structure and preparation method of heart-type fatty acid binding protein detection device

A technology of fatty acid combination and start-up control, which is applied in the field of immunoassay, can solve the problems of uncontrollable reaction start time, unstable immunochromatographic detection results, incomplete and uniform flow, etc., so as to improve the capture and binding ability, improve the application prospect, The effect of improving detection accuracy

Inactive Publication Date: 2022-07-01
CHANGZHOU BIOWIN BIOPHARM
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the existing technology has the following deficiencies: (1) A pain point problem in the industry is that the liquid phase flows unevenly on the solid phase membrane, resulting in unstable immunochromatographic detection results and long detection time
(2) The flow of liquid on the membrane is affected by the characteristics of the membrane and the characteristics of the liquid itself, and the flow process cannot be completely completed within the artificially set time, and the flow is not completely uniform, which affects the detection
(3) The chromatographic reaction begins immediately after sampling, and the reaction start time cannot be controlled artificially. It is necessary to manually record the sampling time of each sample, resulting in a waste of medical resources

Method used

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  • Heart-type fatty acid binding protein detection device with starting control structure and preparation method of heart-type fatty acid binding protein detection device
  • Heart-type fatty acid binding protein detection device with starting control structure and preparation method of heart-type fatty acid binding protein detection device
  • Heart-type fatty acid binding protein detection device with starting control structure and preparation method of heart-type fatty acid binding protein detection device

Examples

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Effect test

Embodiment 1

[0027] Example 1: Preparation of heart-shaped fatty acid-binding protein detection device with activation control structure of the present invention

[0028] (1) Preparation of binding membrane pad

[0029] Dissolve BSA and NaCl with pH 8.0 sodium bicarbonate buffer to a final concentration of 2% BSA and 0.1 M NaCl, then add surfactant Tween20 to a final concentration of 0.5%, adjust pH to 8.0, press glass fiber Plain film water absorption 60uL / cm 2 , spread the above buffer evenly on the glass cellulose membrane, dry at 37°C for 8 hours, and store at 4°C for later use.

[0030] Take 0.5mL of fluorescent microsphere solution with a particle size of 300nm, wash with 2mL of 50mM, pH6.0 MES buffer to remove the surfactant in the microsphere solution, and then dissolve the microspheres to 50mM, pH6.0 MES buffer to 2mL, then activated with 500uL of 10mg / mL activator EDC and 500uL of 50mg / mL stabilizer NHS, then washed with 50mM MES, pH 6.0 to remove the remaining activator, re-di...

Embodiment 2

[0035] Embodiment 2: the present invention and the contrast reagent test result comparison

[0036] (1) Materials: The sample used was a human-type fatty acid-binding protein control with a concentration of 50 ng / ml.

[0037] (2) Add the human-type fatty acid-binding protein control product to 10 control reagent cards and 10 reagent cards of the present invention, respectively, to start the reaction, and use it after 5, 8, 10, 15, 20, and 25 minutes of reaction. The fluorescence detector was used for detection, the detection results were recorded, and the measurement mean and CV value of each time point were calculated.

[0038]

[0039] Table 1 Test results of comparative test 1

[0040] (3) Add the human-type fatty acid-binding protein control substance to 10 control reagent cards 2 and 10 reagent cards of the present invention, respectively, to start the reaction, and use it after 5, 8, 10, 15, 20, and 25 minutes of reaction. The fluorescence detector was used for dete...

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Abstract

The invention discloses a heart-type fatty acid binding protein detection device with a start control structure and a preparation method of the heart-type fatty acid binding protein detection device, and the detection device consists of a fluorescence immune heart-type fatty acid binding protein detection test strip, a lateral flow chromatography start control structure positioned on the detection test strip and a centrifugal device, a liquid phase is driven to enter the solid phase detection membrane through centrifugation and keeps flowing, and then detection reaction is started. The method disclosed by the invention has the outstanding advantages of simplicity and convenience in operation, high detection efficiency, high stability, capability of manually controlling the reaction time and the like, and has important practical significance.

Description

technical field [0001] The invention relates to the technical field of immunodetection, in particular to a heart-shaped fatty acid binding protein detection device with an activation control structure and a preparation method thereof. Background technique [0002] One hour after the onset of acute myocardial infarction (AMI) in critically ill patients is the peak period of death, and the mortality rate is as high as 50%. Long pre-treatment diagnosis time will significantly increase the risk of AMI death. Therefore, improving the diagnosis speed of AMI is of great significance in saving people's lives and promoting people's health. The "Guiding Principles for the Construction and Management of Chest Pain Centers" issued by the National Health and Family Planning Commission clearly pointed out that shortening the time from blood drawing to obtaining the report of the heart-type fatty acid binding protein detection project, and listed it as a basic indicator for medical quality...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/533G01N33/558G01N33/577G01N33/68
CPCG01N33/54313G01N33/533G01N33/558G01N33/577G01N33/6893G01N2800/324
Inventor 周伟曹健成艳
Owner CHANGZHOU BIOWIN BIOPHARM
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