IL-6 biotin-streptavidin immunochromatographic detection card

A technology of streptavidin and IL-6, which is applied in the field of disease detection, can solve the problems of low detection sensitivity of low-end values ​​and low accuracy of detection results, so as to improve the performance of the detection card, improve the detection sensitivity, and highly Effects of Specificity and Stability

Pending Publication Date: 2019-12-20
CHENGDU RENZHI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The invention provides an IL-6 biotin-streptavidin immunochromatographic detection card to improve the low-end value detection sensitivity and low detection result accuracy in the prior art

Method used

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  • IL-6 biotin-streptavidin immunochromatographic detection card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Prepare conventional IL-6 fluorescence immunochromatography detection card, the preparation process is as follows:

[0053] 1. Preparation of coating film 3: Dilute the goat anti-mouse IgG antibody to a concentration of 1.0mg / mL with 0.01M PBS buffer solution with a pH value of 7.4 to prepare the quality control C-line coating solution; the mouse anti-human IL-6 The antibody was diluted to a concentration of 1 mg / mL to prepare a T-line detection coating solution. The spray volume is 1.0uL / cm, and the film-drawing speed is 100mm / s. Coat the C-line coating solution and the T-line coating solution on a blank NC membrane (nitrocellulose membrane) to form a quality control C-line 4 and detect T line 5, put it into an oven at 37°C, and dry it overnight to obtain coating film 3.

[0054] 2. Preparation of binding pad 6: After washing and activating the fluorescent microspheres, add 0.1 mg of mouse anti-human IL-6 antibody per 0.1 mL of fluorescent microspheres for labeling, s...

Embodiment 2

[0059] Preparation of IL-6 biotin-streptavidin immunochromatographic detection card, the preparation process is as follows:

[0060] 1. Preparation of coating film 3: Dilute the goat anti-mouse IgG antibody to a concentration of 1.0 mg / mL with 0.01M PBS buffer solution with a pH value of 7.4 to prepare a quality control C-line coating solution; Streptavidin was diluted to a concentration of 1 mg / mL to prepare a T-line detection coating solution. The spray volume is 1.0uL / cm, and the film-drawing speed is 100mm / s. Coat the C-line coating solution and the T-line coating solution on the blank NC film to form the quality control C line 4 and the detection T line 5. The coating film 3 was obtained by drying overnight in an oven at 37°C.

[0061] 2. Preparation of binding pad 6: Spray the solution of IL-6 monoclonal antibody coupled with fluorescent microspheres and anti-IL-6 monoclonal antibody coupled with biotin onto the glass fiber carrier, put it in an oven at 37°C, and dry ov...

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Abstract

The invention discloses an IL-6 biotin-streptavidin immunochromatographic detection card, which belongs to the technical field of disease detection. The IL-6 biotin-streptavidin immunochromatographicdetection card comprises a PVC bottom plate, a sample pad, a combination pad, a coating film and water absorbing paper, wherein the sample pad, the combination pad, the coating film and the water absorbing paper are sequentially arranged on the PVC bottom plate from front to back, a detection T line and a quality control C line are arranged on the coating film, and an anti-IL-6 monoclonal antibodycoupled with a tracing marker and an anti-IL-6 monoclonal antibody coupled with biotin are arranged on the combination pad; and the detection T line is coated with streptavidin, and the quality control C line is coated with a polyclonal antibody. The IL-6 biotin-streptavidin immunochromatographic detection card quantitatively detects the content of IL-6 in human serum, blood plasma and whole blood samples by utilizing an immunochromatographic technology according to the principle of a double-antibody sandwich method, can obtain the concentration of IL-6 in the samples precisely, and improvesthe detection sensitivity, particularly the detection sensitivity of a low-end value.

Description

technical field [0001] The invention belongs to the technical field of disease detection, and in particular relates to an IL-6 biotin-streptavidin immunochromatographic detection card. Background technique [0002] IL-6 is an inflammatory factor and a pleiotropic cytokine with a wide range of functions. After the body is stimulated by inflammation, it is secreted by T cells, B cells, monocytes and macrophages, and endothelial cells. It is the key to the network of inflammatory mediators. Element. After the inflammatory response occurs, IL-6 is produced first, and then induces the production of CRP and procalcitonin (PCT). For example, it will be rapidly produced during the acute inflammatory response of infection, internal and external trauma, surgery, stress response, brain death, tumor generation and other situations. IL-6 is involved in the occurrence and development of many diseases, and its blood level is closely related to inflammation, viral infection, and autoimmun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/577G01N33/558G01N33/533G01N33/532
CPCG01N33/6863G01N33/558G01N33/532G01N33/533G01N33/58G01N33/582G01N33/587G01N33/588G01N33/577
Inventor 谭四军贺率夏斌
Owner CHENGDU RENZHI TECH
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