36results about How to "Short sequence length" patented technology

This invention relates to the unexpected discovery that nucleotide coding sequences coding for a truncated Epstein Barr Nuclear Antigen 1 (EBNA1t) protein (lacking the Gly-Gly-Ala domain), when in cells of mammalian origin, are associated with improved growth and increased transient gene expression when compared with cells expressing a complete EBNA1 coding sequence. The expression of EBNA1t also appear to be more stable over time.

The invention discloses a nucleic acid aptamer capable of detecting myohemoglobin and a derivative of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer comprises a DNA segment shown by any one sequence in the sequences from sequence 1-sequence 5 shown in the description. The invention discloses a microfluidic chip which comprises a sample inlet, a sample outlet and a sample feeding channel. The sample feeding channel is divided by two narrow channels into front, middle and rear channels. The front channel is filled with reverse screening protein modified microspheres, and the middle channel is filled with positive screening protein modified microspheres, wherein the grain size of microspheres is greater than width of the narrow channels. The invention further discloses a screening method for screening the nucleic acid aptamer by using the microfluidic chip. The method comprises the main steps of optimizing a nucleic acid library, screening initially, purifying, and circulating. The detection method provided by the invention has the advantages of high affinity and specificity.

Synthesis of degradable aromatic/fatty copolymer ester by low lactic acid in situ ester exchange is carried out by mixing AAC and DO with D, L-PLA in proportion of 1:1: (0-1.0), copoly-condensing without solvent or with diphenyl ether as solvent and synthesizing the final product.

The invention discloses uPAR (urokinase-type plasminogen activator receptor) targeted peptides with the amino acid sequence indicated in SEQ ID NO. 1. The uPAR targeted peptides is linear peptides formed by 13 amino acids, the peptides are easy to synthesize and can form a stable conformation and be combined with uPARs with specific target. The invention discloses a uPAR targeted probe comprisinga single unit and the above uPAR targeted peptides. The uPAR targeted probe is subjected to specific identification of the peptides and is combined with the uPARs, signals for detection of imaging equipment are transmitted through the signal unit, living visual detection of the uPARs is achieved, and distribution and number of the uPARs are displayed visually and are used for disease detection foruPAR expression abnormalities. The invention further discloses a living molecular imaging method. The uPAR targeted probe is utilized, and the living molecular imaging method has the advantages of being safe, non-invasive, dynamic, visual and the like and is suitable for direct detection of human body.

The invention provides a beta hairpin antibacterial peptide with tryptophan and arginine cross-chain interaction and a preparation method of the beta hairpin antibacterial peptide. The sequence of theantibacterial peptide WRLPG is shown as SEQ ID No. 1. The preparation method comprises the following step: designing an antibacterial peptide template XWYRYPGXWYRY-NH2 containing tryptophan and arginine cross-chain interaction and a PG corner unit on the basis of beta-hairpin amphiphilic peptide arrangement, wherein X= R, and Y= L. The invention also provides application of the antibacterial peptide in preparation of medicines for treating infectious diseases caused by gram-positive bacteria or/and gram-negative bacteria. The antibacterial peptide forms a stable beta-hairpin structure under the condition that a disulfide bond formed by cysteine pairs is not contained, the length of an amino acid sequence is only 12, the antibacterial peptide has excellent biosecurity on the basis of maintaining high antibacterial activity, and the treatment index reaches 90.78. The antibacterial peptide has high development potential of replacing antibiotics.

The invention discloses a single-stranded DNA aptamer specifically recognizing tobramycin and application thereof, and belongs to the technical field of biology. On the basis of ap32-34nt, by removingsome of terminal base pairs and unpaired bases and shortening the stem region length of a secondary structure, a tobramycin aptamer ap32-15nt with the greatly shortened sequence length and the improved affinity is obtained; the aptamer ap32-15nt is applied to construction of various tobramycin detection methods and detection reagents; the aptamer has the advantages of high affinity, high specificity, a stable structure and the like; and the constructed detection methods have the advantages of a short detection period, high sensitivity, low cost, strong specificity and the like.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide YHX-3 and a composition and application thereof, and an amino acid sequence of the antibacterial peptide YHX-3 is as shown in SEQ ID No.1. The antibacterial peptide YHX-3 provided by the invention has broad-spectrum antibacterial activity, and has remarkable antibacterial activity on gram-positive bacteria and gram-negative bacteria including listeria monocytogenes, staphylococcus aureus, streptococcus mutans, escherichia coli and salmonella; and the antibacterial peptide has low hemolytic activity, a small sequence length, low molecular weight, and low chemical synthesis difficulty, and reduces large-scale production cost very well while killing pathogenic bacteria in organisms more specifically,.

The invention provides an isothermal amplification system and method of a fluorescence self-inhibition probe. The isothermal amplification system comprises a combination of two fluorescence self-inhibition probes, a Vent DNA polymerase, an Nt.BstNBI nicking endonuclease, a buffer solution, a reaction raw material and miRNA target molecules. The fluorescence self-inhibition probes comprise a fluorescence self-inhibition linear probe and a fluorescence self-inhibition hairpin probe. The invention also provides an amplification method of the isothermal amplification system. According to the fluorescence self-inhibition probe isothermal amplification system and the amplification method, background fluorescence signals of the probe can be remarkably reduced, the detection signal-to-noise ratio is greatly increased, and detection of trace miRNA targets in a sample is facilitated. Meanwhile, no complementary sequence exists between the two background-free fluorescent probes, mismatching and extension between the probes can be prevented, the detection specificity is effectively improved, false positive results are avoided, and the system and method are particularly suitable for direct detection of miRNA molecular markers in clinical samples.

The invention provides a blood group antibody detection method, a blood group antibody detection kit, a blood group antibody binding protein, nucleic acid for coding the blood group antibody binding protein and a carrier containing the nucleic acid. Particularly, the blood group antibody binding protein comprises more than 80% of homology with SEQ ID NO: 2, 4, 6, 8 or 10 or comprises an amino acid sequence shown as SEQ ID NO: 2, 4, 6, 8 or 10, has antigen specificity, and can be used for blood group antibody detection.

The invention discloses a lightweight time convolution network for quick prediction of time series data. An overall thought framework and a specific structure of the lightweight time convolution network are improved, expansion convolution is replaced by one-dimensional step convolution, and a full convolution structure is removed; as the calculated amount of the lightweight time convolution network provided by the invention is greatly reduced, and all calculation results in the overall thought framework of the lightweight time convolution network are used in forward and backward propagation ofthe network, the calculation waste is reduced; and meanwhile, compared with the prior art, the calculated amount required by the network is also greatly reduced; and meanwhile, the depth of the network can be effectively reduced by using a relatively large convolution kernel and a relatively large convolution step length.

The present invention provides a beta-hairpin antimicrobial peptide with cross-chain interaction between tryptophan and histidine and a preparation method of the beta-hairpin antimicrobial peptide. The antimicrobial peptide WHFPG has a sequence shown in SEQ ID No.1. The preparation method utilizes an arrangement principle of a beta-hairpin structure, the interaction between the tryptophan and histidine forms mutual attraction of two beta-side chains, a PG corner is used as a central corner unit to obtain a stable beta-hairpin amphiphilic antimicrobial peptide template XWYHYPGXWYHY-NH2, whereinX=R and Y=F. The antimicrobial peptide is applied in preparation of medicines for treating infectious diseases caused by gram-positive bacteria or/and gram-negative bacteria. The antimicrobial peptide can work under both a neutral pH condition and an acidic pH condition, can inhibit the gram-negative bacteria more powerfully under the acidic pH condition, has a therapeutic index of 211.68, and has a wider clinical application potential.

The invention relates to the field of polypeptide drugs, in particular to a stapled peptide conjugate for degrading MDM2/MDMX protein by protein-targeted chimeras (PROTACTs) and a preparation method and application thereof. The stapled peptide conjugate is composed of three parts: a first peptide fragment suitable for binding with the MDM2/MDMX protein, a second peptide fragment suitable for binding with E3 ubiquitin ligase VHL, and a connecting arm enabling the first peptide fragment and the second peptide fragment to be connected. The E3 ubiquitin ligase VHL can be used for carrying out ubiquitination modification on the MDM2/MDMX protein, so that protease degrades the ubiquitination MDM2/MDMX protein, a P53 factor is activated to play roles in killing cancer cells and inhibiting tumor formation ability, and the E3 ubiquitin ligase VHL has a potential application value.

Provided by the present invention is a mutant lysyl-tRNA synthetase that has a deletion mutation at one or more sites selected from the group corresponding to the amino acid sequence of wild-type aminoacyl-tRNA synthetase: 102nd, 128th-140th, and 159th-179th amino acid residues. Compared with wild-type lysyl-tRNA synthetase, the mutant lysyl-tRNA synthetase according to the present invention has high activity, a high level of expression and good solubility, and may significantly increase the amount of an inserted unnatural amino acid and the level of expression of a target protein containing an unnatural amino acid.