Test strip capable of quantificationally detecting content of c-reactive protein in peripheral blood and preparation method thereof

A reactive protein and quantitative detection technology, which is applied in the field of quantum dot immunofluorescence chromatography in medical immunology, can solve the problems that the accuracy and repeatability of CRP kits need to be improved, so as to reduce the interference of human subjective factors, improve performance, The effect of improving stability

Pending Publication Date: 2019-01-25
重庆新赛亚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the accuracy and reproducibility of current CRP kits still need to be improved

Method used

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  • Test strip capable of quantificationally detecting content of c-reactive protein in peripheral blood and preparation method thereof
  • Test strip capable of quantificationally detecting content of c-reactive protein in peripheral blood and preparation method thereof
  • Test strip capable of quantificationally detecting content of c-reactive protein in peripheral blood and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the preparation of detection reagent card

[0041] 1. Optimization of sample pad 5:

[0042] 1.1. Materials and reagents:

[0043] (1) Sample pad 5: made of glass fiber.

[0044] (2) Sample pad 5: Hangzhou Najing Biotechnology Co., Ltd.

[0045] (3) Sample pad optimization buffer: 50mmol / L, Tris (Tris) at pH 8.2, Tween 20 with a concentration of 0.5% by mass, proclin300 with a concentration of 0.1% by mass, and The balance of purified water.

[0046] 1.2. Immerse the sample pad 5 in the sample pad optimization buffer solution for 30-45 minutes, then dry it in the drying room for more than 5 hours, and control the humidity below 30%.

[0047] 2. Optimization of Quantum Pad 4:

[0048] 2.1. Materials and reagents

[0049] (1) Quantum pad 4: glass fiber material.

[0050] (2) Quantum Mat 4: Hangzhou Najing Biotechnology Co., Ltd.

[0051] (3) Quantum pad optimization buffer: 50mmol / L, tris(Tris) of pH8.2, bovine serum albumin (BSA) with a mass percent...

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Abstract

The invention relates to a test strip capable of quantificationally detecting content of c-reactive protein in peripheral blood. The test strip comprises a sample cushion, a quantum cushion, a nitrocellulose membrane, water absorbing paper and a lining plate, the quantum cushion wraps c-reactive protein antibodies marked by carbon quantum dots, and a detecting line and a quality control line are successively arranged on the nitrocellulose membrane from the sample cushion side to the water absorbing paper side; the detecting line wraps c-reactive protein recognizing monoclonal antibodies, and the quality control line wraps goat anti mouse IgG. The test strip is simple in structure and can quickly and accurately detect CRP in the peripheral blood quantificationally, the detection of the teststrip is high in sensitivity and stability and good in specificity, the detecting time is shorter than 15 min, and the diagnostic efficiency and the work efficiency of detecting operators can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of quantum dot immunofluorescence chromatography in medical immunology, and in particular relates to a test strip capable of quantitatively detecting the content of C-reactive protein in peripheral blood and a preparation method thereof. Background technique [0002] CRP (C-reactive protein) is the first acute phase reaction protein discovered in the body. It is generally considered to be a very sensitive marker of inflammation and tissue damage. Tillett and Francis first discovered in 1930 that a serum of patients with pneumonia was associated with Streptococcus pneumoniae (now called Streptococcus pneumoniae) non-type-specific bacterial polysaccharides are substances that undergo precipitation reactions into C polysaccharides. In 1941, Macleod and Avery successively confirmed that this is a protein that appears during acute infection, and the flocculent precipitation reaction with C polysaccharides depends...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558
CPCG01N33/558G01N33/68
Inventor 左云国赵亚丽
Owner 重庆新赛亚生物科技有限公司
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