Kit for quickly determining content of glycocholic acid (CG) in serum or blood plasma

A technology for detecting kits and glycocholic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor accuracy, sensitivity, and repeatability, and achieve the effects of small hemolytic interference, accurate results, and high specificity

Active Publication Date: 2015-02-18
ZYBIO INC
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the accuracy, sensitivity and repeatability of glycocholic acid detection kits currently on the market in my country are still poor.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for quickly determining content of glycocholic acid (CG) in serum or blood plasma
  • Kit for quickly determining content of glycocholic acid (CG) in serum or blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation of test paper card of the present invention:

[0048] 1) Use the pretreatment buffer to pretreat the gold standard pad. The pretreatment buffer is: stachyose 2g / L, alum 0.5g / L, fructose sodium diphosphate 1.5g / L, sodium hexametaphosphate 0.3g / L L, glycine 1.88g / L aqueous solution, pH = 7.4, the specific steps of pretreatment are: soak the gold standard pad in the pretreatment solution for 2 hours, take it out and dry it at 37°C; Acid (CG) antibody solution was sprayed on the pretreated gold label pad to obtain a gold label pad coated with glycocholic acid (CG) antibody. The mass ratio of fluorescent microspheres and antibody in the solution was 5:1, and the concentration of the solution was 10mg / ml, the spray volume is 4ul / cm;

[0049] 2) Spray 1 mg / ml of glycocholic acid (CG) antibody solution and goat anti-mouse antibody solution on the detection line and the quality control line of the nitrocellulose membrane respectively, and the spraying amount is 1ul / ...

Embodiment 2

[0063] Preparation of comparative test paper card:

[0064] The gold standard pad was pretreated with 25mM glycine buffer solution, and other reagents and experimental methods were the same as in Example 1.

[0065] 1) Pretreat the gold label pad with 25mM glycine buffer solution, and then spray the pretreated gold label pad with the glycocholic acid (CG) antibody solution labeled with fluorescent microspheres to prepare the gold label pad coated with the glycocholic acid (CG) antibody. Standard pad, the mass ratio of fluorescent microspheres and antibodies in the solution is 5:1, the concentration of the solution is 10mg / ml, and the spraying amount is 4ul / cm;

[0066] 2) Spray 1 mg / ml of glycocholic acid (CG) antibody solution and goat anti-mouse antibody solution on the detection line and the quality control line of the nitrocellulose membrane respectively, and the spraying amount is 1ul / cm to obtain the coated nitric acid Cellulose film;

[0067] 3) Paste the sample pad, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of biological detection and in particular relates to a detection kit used for quantitatively detecting glycocholic acid (CG) as well as a preparation method and application of the detection kit. The detection kit used for quantitatively detecting CG comprises a test paper card, wherein the test paper card comprises a base plate as well as a sample pad, a gold mark pad, a nitrocellulose membrane and a water absorption pad which are sequentially arranged from a sampling end positioned on the surface of the base plate, the gold mark pad contains a CG antibody, the nitrocellulose membrane is coated with a detection line and a quality control line, and the CG antibody on the gold mark pad is marked by adopting a fluorescent microsphere. The detection kit provided by the invention can be used for detecting CG by adopting a fluorescent microsphere immunochromatographic technology initially, has sensitivity and specificity and has the advantages of quickness and simplicity in operation, accurate result, economic applicability and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a kit for rapidly measuring the content of glycocholic acid in serum or plasma, its preparation method and application. Background technique [0002] Cholyglycine (CG) is a conjugated bile acid formed by the combination of bile acid and glycine. In liver cells, cholesterol is transformed into primary bile acid through complex enzymatic reactions. Among them are cholic acid (CA) and chenodeoxycholic acid (CD-CA). There are three hydroxyl groups (C3, C7, C12) on the steroid core of cholic acid, and the hydroxyl group at the end of the side chain is combined with glycine through a peptide bond. [0003] CG is synthesized by liver cells, and its normal metabolic pathway is enterohepatic circulation. It is discharged into the gallbladder through the capillary and bile ducts, and enters the duodenum along with bile to help digest food. 95% of bile acid is reabsorbed in the termina...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 陈亨宇
Owner ZYBIO INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap