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Biochip and method capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins

A biotoxin and biochip technology, which is applied to the simultaneous visual detection of biotoxin residues, multiple antibiotics, and illegal additives, can solve the problems of long time-consuming microbial methods and inability to quickly detect, and achieve short detection time, simple structure, and high preparation technology. simple effect

Active Publication Date: 2013-08-21
NANJING XIANGZHONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all require heating equipment to cultivate microorganisms, and the microbial method takes a long time and cannot be quickly detected

Method used

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  • Biochip and method capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins
  • Biochip and method capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins
  • Biochip and method capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Simultaneous quantitative detection of kanamycin, cephalexin, gentamicin, sulfamethazine, tetracycline, erythromycin, ciprofloxacin, furaltadone, chloramphenicol, lincomycin, melamine, Aflatoxin M1, T-2 toxin, zearalenone and fumonisins content.

[0051] The reagents used in the experiment are as follows:

[0052] (1) Standard solution:

[0053] There are 6 kinds of mixed standard solutions used in the present embodiment, and the composition is as follows:

[0054]①Mixed standard solution 1: the concentration of kanamycin is 0ng / mL, the concentration of cephalexin is 0ng / mL, the concentration of gentamycin is 0ng / mL, the concentration of sulfamethazine is 0ng / mL, and the concentration of tetracycline is 0ng / mL / mL, the concentration of erythromycin is 0ng / mL, the concentration of ciprofloxacin is 0ng / mL, the concentration of furaltadone is 0ng / mL, the concentration of chloramphenicol is 0ng / mL, and the concentration of lincomycin is 0ng / mL. The concentration of melam...

Embodiment 2

[0096] Simultaneous quantitative detection of gentamicin, tetracycline, oxytetracycline, chlortetracycline, clarithromycin, sulfadiazine, norfloxacin, ciprofloxacin, ofloxacin, Sudan red, trichothecenes in pork , The content of vomitoxin.

[0097] The detection steps are as follows:

[0098] (1) Preparation of biochip: Using bovine serum albumin as a blank control, a biochip preparation instrument (BioDot, USA) was used to prepare a microarray chip on a 96-well plate, and bovine serum albumin, gentamicin, Tetracycline, oxytetracycline, chlortetracycline, clarithromycin, sulfadiazine, norfloxacin, ciprofloxacin, ofloxacin, Sudan red, trichothecenes, and vomitoxin antigens were spotted on 96-well plates For the same operation, fix in a water bath at 20°C for 4 hours, and form a microarray structure on a 96-well plate, and the array spots at different positions correspond to different antigens;

[0099] (2) Preparation of sample solution to be tested: Take 5 g of crushed pork s...

Embodiment 3

[0104] Simultaneous quantitative detection of penicillin, streptomycin, oxytetracycline, aureomycin, clarithromycin, sulfadiazine, sulfadimethoxine, norfloxacin, ofloxacin, furazolidone, thiamphenicol, Malachite green, aflatoxin content.

[0105] (1) Preparation of biochip: using bovine serum albumin as a blank control, use a biochip preparation instrument (BioDot, USA) to prepare a microarray chip on a 96-well plate, and bovine serum albumin, penicillin, streptomycin , oxytetracycline, chlortetracycline, clarithromycin, sulfadiazine, sulfadimethoxine, norfloxacin, ofloxacin, furazolidone, thiamphenicol, malachite green, aflatoxin antigen in 96 wells Spotting on the plate, fixed in a water bath at 30°C for 2 hours, and formed a microarray structure on a 96-well plate, and the array spots at different positions corresponded to different antigens;

[0106] (2) Preparation of sample solution to be tested: take 5 g of pulverized shrimp meat, add 8 mL of buffer, mix for 2 min, pla...

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Abstract

The invention provides a biochip capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins. The biochip comprises a chip carrier for fixing a group of detection target object antigens, wherein detection target objects are the antibiotics, the illegal addition agents and the biotoxins; the biochip is prepared by using a method; and the method comprises the following steps of: performing sample operation on bovine serum albumin for blank control and the detection target object antigens on the chip carrier through a biochip preparing system, and fixing the bovine serum albumin and the detection target object antigens in a 20-37 DEG C water bath for 0.5-4h, so as to obtain the biochip. The invention also provides a method capable of simultaneously visually detecting the multiple antibiotics, illegal addition agents and biotoxins by utilizing the biochip. The biochip provided by the invention has the advantages that the structure is simple, the preparation process is simple, the cost is low, multiple targets are arranged, the accuracy is high, the sensitivity is high, the precision is high, the detection time is short, the operation is simple and easy, an expensive detecting instrument is not needed, and the biochip is suitable for sieving field large-scale samples.

Description

technical field [0001] The invention belongs to the technical field of immunoanalysis for food safety supervision or food analysis, in particular to a method for simultaneous visual detection of multiple antibiotics, illegal additives and biotoxin residues. Background technique [0002] Antibiotics are a class of secondary metabolites with anti-pathogen or other activities produced by microorganisms (including bacteria, fungi, actinomycetes) or higher animals and plants in the course of life, which can interfere with the developmental functions of other living cells The commonly used antibiotics include extracts from microbial culture fluid and chemically synthesized or semi-synthesized compounds. Antibiotics are widely used as antibiotics and feed additives, which may cause antibiotic residues in food of animal origin. For dairy products such as milk, antibiotic residues will not only affect the quality of dairy products, such as seriously affecting the production of ferme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/6845G01N33/94G01N2415/00G01N33/9446
Inventor 李钟卉李周敏孙思云
Owner NANJING XIANGZHONG BIOTECH
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