Detecting reagent kit used for detecting quinoxalinone-2-carboxylic acid and method

A detection method and quinoxaline technology, which are applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of cumbersome and time-consuming procedures, expensive instrument purchases, and inability to operate on-site, and achieve simple sample pretreatment and strong detection specificity. , a wide range of effects

Inactive Publication Date: 2012-09-05
CHONGQING ACADEMY OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrument testing mainly has defects such as expensive instrument purchase, complicated sample pretreatment, cumbersome and time-consuming procedures, high testing costs, and inability to operate on-site, so its application in production is limited
There are currently no patents and literature reports on a time-resolved fluorescent immunoassay for the detection of quinoxaline-2-carboxylic acid residues

Method used

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  • Detecting reagent kit used for detecting quinoxalinone-2-carboxylic acid and method
  • Detecting reagent kit used for detecting quinoxalinone-2-carboxylic acid and method
  • Detecting reagent kit used for detecting quinoxalinone-2-carboxylic acid and method

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Embodiment Construction

[0024] Preparation of 1-quinoxaline-2-carboxylic acid (QCA-carrier protein conjugate

[0025] Synthesis of immunogen (QCA-BSA): Accurately weigh 348 mg of quinoxaline-2-carboxylic acid and dissolve it in 2 mL of N,N-dimethylformamide, add γ-aminobutyric acid solution drop by drop under stirring, and stir for 3 hours , adjust the pH of the reaction solution to about 10. The precipitate was removed by centrifugation. Add the above reactants dropwise to the BSA solution (340mgBSA dissolved in 5mL physiological saline), then add 23mg of N-hydroxysuccinimide (NHS), 43.4mg of N,N-dicyclohexylcarbodiimide (DCC) , reacted overnight at 4°C, centrifuged to remove the precipitate, and the supernatant was dialyzed against phosphate buffered solution (PBS) for 3 days, and the dialysate was changed every 6 hours. The resulting product was lyophilized and stored at -20°C for future use.

[0026] Synthesis of immunogen (QCA-OVA): Accurately weigh 348 mg of quinoxaline-2-carboxylic acid and ...

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Abstract

The invention discloses a time-resolved fluorescence immunoassay reagent kit which is used for detecting quinoxalinone-2-carboxylic acid and a detection method of the time-resolved fluorescence immunoassay reagent kit. The reagent kit comprises a porous coated plate, a buffer solution, quinoxalinone-2-carboxylic acid standard, freeze-dried antibodies of quinoxalinone-2-carboxylic acid, europium-marked goat anti mouse antibodies, a cleaning solution and an enhancement solution. By measuring fluorescence intensity of counts per second (cps), the content of quinoxalinone-2-carboxylic acid in samples is calculated by a standard curve. The kit provided by the invention has the advantages of simple structure, easiness in assembly, corrosion resistance, light weight, low cost, wide application and the like. A valve core has good centering performance and can move flexibly and reliably and can play a good water-plugging role.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a detection kit for detecting quinoxaline-2-carboxylic acid and a method thereof. Background technique [0002] As an antibacterial and growth-promoting agent, carbadoxy is widely used in livestock and poultry breeding, which can promote animal growth, improve feed conversion rate, prevent and control swine dysentery and bacterial enteritis. Metabolic and toxicological studies have shown that carbadox rapidly transforms into deoxygenation and deoxygenation compounds in pigs, and its prototype and deoxygenation compounds have certain toxic effects on humans and animals, mainly manifested as carcinogenicity and mutagenesis , Reproductive toxicity. Therefore, in 1999, the European Union banned the application of carbad oxygen in food animals. In 2001, Canada also issued a decree to prohibit the sale of carbad oxygen in the market. In 2003, the Joint Expert Committee ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 乐涛贾渝跃何红秋李滨牛小东陈雍王玉段小炼秦建波
Owner CHONGQING ACADEMY OF SCI & TECH
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