Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof

A technology of Staphylococcus saprophyticus and detection kit, which is applied in biological testing, measuring devices, material inspection products, etc. It can solve the problems of serum antibody detection technology that has not been reported, and achieve the effect of simple, convenient, economical and low cost

Inactive Publication Date: 2018-12-07
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are very few research reports on Staphylococcus saprophyticus at home and abroad. The molecular biology detection technology for Staphylococcus saprophyticus mainly relies on...

Method used

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  • Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof
  • Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof
  • Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof

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Embodiment 1

[0037] The indirect hemagglutination kit for detecting Staphylococcus saprophytic serum antibody involved in the present invention comprises:

[0038] Staphylococcus saprophytic indirect hemagglutination antigen 1 bottle (5ml), stored at 4°C;

[0039] 1) 1 bottle (0.5ml) of standard positive serum, frozen at -20°C;

[0040] 2) 1 bottle (0.5ml) of standard negative serum, frozen at -20°C;

[0041] 3) 3 bottles of diluent (5ml / bottle), stored at 4°C.

[0042]The Staphylococcus saprophyticus strain MC2016 used to prepare the antigen is a field isolate and is preserved in the State Key Laboratory of Livestock Disease Pathogen Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0043] The preparation method of various reagents in the kit of the present invention is:

[0044] 1. Indirect hemagglutination antigen

[0045] Staphylococcus saprophyticus CCTCC M 2018117 strain was enriched and cultured, ultrasonically crushed, and then centrifu...

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Abstract

The invention discloses an indirect coagulation kit for detecting a serum antibody against Staphylococcus saprophyticus, and methods for preparing and using the kit. The kit comprises (1) the indirecthemagglutination antigen of Staphylococcus saprophyticus, (2) standard positive serum, (3) standard negative serum, and (4) a diluent. The invention also provides methods for preparing and using thekit. The invention provides the detection kit for the indirect coagulation antibody against Staphylococcus saprophyticus and application thereof. The kit is simple and convenient to operate, and is extensively applicable to various veterinary-related units and farms; the kit in the invention only needs simple instrument consumables during detection of samples, and the whole detection process can be completed in 2-3 hours; results are intuitive and reproducible; and the kit can realize fast, stable and accurate detection of antibodies against Staphylococcus saprophyticus.

Description

technical field [0001] The invention relates to a staphylococcus saprophytic indirect hemagglutination antibody detection kit and a preparation method thereof, belonging to the technical field of detection reagents for microorganisms. Background technique [0002] Staphylococcus saprophyticus belongs to the genus of coagulase-negative Staphylococcus and is a Gram-positive coccus widely distributed in nature and on the human body surface. Staphylococcus saprophyticus can be detected in infected specimens, which has important clinical significance. At the same time, Staphylococcus saprophyticus is also the main pathogenic bacteria of latent mastitis in dairy cows, and it is also a common bacterial species in Greek sausage, salted duck and traditional fermented meat of Dong nationality in China. At present, there are very few research reports on Staphylococcus saprophyticus at home and abroad. The molecular biology detection technology for Staphylococcus saprophyticus mainly r...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 宫晓炜陈启伟郑福英刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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