Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof

A technology of Staphylococcus saprophyticus and detection kit, which is applied in biological testing, measuring devices, material inspection products, etc. It can solve the problems of serum antibody detection technology that has not been reported, and achieve the effect of simple, convenient, economical and low cost

Inactive Publication Date: 2018-12-07
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few research reports on Staphylococcus saprophyticus at home and abroad. The molecular biology detection technology for Staphylococcus saprophyticus mainly relies on laboratory bacterial isolation and PCR molecular biology identification. The serum antibody detection technology for Staphylococcus saprophyticus is not available at home and abroad. see the report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof
  • Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof
  • Detection kit for indirect coagulation antibody against Staphylococcus saprophyticus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The indirect hemagglutination kit for detecting Staphylococcus saprophytic serum antibody involved in the present invention comprises:

[0038] Staphylococcus saprophytic indirect hemagglutination antigen 1 bottle (5ml), stored at 4°C;

[0039] 1) 1 bottle (0.5ml) of standard positive serum, frozen at -20°C;

[0040] 2) 1 bottle (0.5ml) of standard negative serum, frozen at -20°C;

[0041] 3) 3 bottles of diluent (5ml / bottle), stored at 4°C.

[0042]The Staphylococcus saprophyticus strain MC2016 used to prepare the antigen is a field isolate and is preserved in the State Key Laboratory of Livestock Disease Pathogen Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0043] The preparation method of various reagents in the kit of the present invention is:

[0044] 1. Indirect hemagglutination antigen

[0045] Staphylococcus saprophyticus CCTCC M 2018117 strain was enriched and cultured, ultrasonically crushed, and then centrifu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an indirect coagulation kit for detecting a serum antibody against Staphylococcus saprophyticus, and methods for preparing and using the kit. The kit comprises (1) the indirecthemagglutination antigen of Staphylococcus saprophyticus, (2) standard positive serum, (3) standard negative serum, and (4) a diluent. The invention also provides methods for preparing and using thekit. The invention provides the detection kit for the indirect coagulation antibody against Staphylococcus saprophyticus and application thereof. The kit is simple and convenient to operate, and is extensively applicable to various veterinary-related units and farms; the kit in the invention only needs simple instrument consumables during detection of samples, and the whole detection process can be completed in 2-3 hours; results are intuitive and reproducible; and the kit can realize fast, stable and accurate detection of antibodies against Staphylococcus saprophyticus.

Description

technical field [0001] The invention relates to a staphylococcus saprophytic indirect hemagglutination antibody detection kit and a preparation method thereof, belonging to the technical field of detection reagents for microorganisms. Background technique [0002] Staphylococcus saprophyticus belongs to the genus of coagulase-negative Staphylococcus and is a Gram-positive coccus widely distributed in nature and on the human body surface. Staphylococcus saprophyticus can be detected in infected specimens, which has important clinical significance. At the same time, Staphylococcus saprophyticus is also the main pathogenic bacteria of latent mastitis in dairy cows, and it is also a common bacterial species in Greek sausage, salted duck and traditional fermented meat of Dong nationality in China. At present, there are very few research reports on Staphylococcus saprophyticus at home and abroad. The molecular biology detection technology for Staphylococcus saprophyticus mainly r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 宫晓炜陈启伟郑福英刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com