Detection method of actinobacillus pleuropneumoniae in pig serums

A technology of porcine pleuropneumonia and actinobacillus, which is applied in the field of pig serum detection, can solve the problems of error, long cycle, and unsuitable application at the grassroots level, and achieve the effect of accurate detection results, stable detection results and low cost

Inactive Publication Date: 2013-08-28
TIANJIN TIANZE LIVESTOCK & POULTRY BREEDING SPECIALIZED COOP
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Problems solved by technology

The etiological diagnosis method requires bacterial isolation, culture and identification, and the cycle is long; the serological diagnosis method includes complement fixation reaction, fluorescent antibody, indirect hemagglutination test and enzyme-linked immunosorbent assay (ELISA), etc. The disadvantages of the serological diagnosis method are It can only detect the antibody of Actinobacillus pleuropneumoniae in animals but cannot detect the pathogen; the molecular biology diagnosis method mainly includes reverse transcription polymerase chain reaction (RT-PCR) and fluorescent nucleic acid probe. Th

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Embodiment Construction

[0029] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0030] The methods used in the present invention, unless otherwise specified, are conventional methods; the reagents and kits used, unless otherwise specified, are commercially available products.

[0031] The general idea of ​​the detection method of the present invention is: for the serum samples of piglets and fattening pigs randomly selected from large-scale farms, the collected serum samples are first detected by the IHA (indirect hemagglutination test) method, and then the collected serum samples are re-examined by the APPELISA test , statistical laboratory test results, analysis and determination of porcine infectious pleuropneumonia situation.

[0032] 1. Preparation before testing

[0033] 1. Pig serum material collection location:...

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Abstract

The invention relates to a detection method of actinobacillus pleuropneumoniae in pig serums. The detection method comprises the steps as follows: randomly selecting serum samples of piglets and finishing pigs in a scale farm, firstly carrying out IHA (indirect hemagglutination test) method detection on the collected serum samples, and secondly carrying out APPELISA detection on the collected serum samples to detect the actinobacillus pleuropneumoniae in the pig serums. According to the detection method, the APP indirect hemagglutination test and the APPELISA test are jointly adopted, so that the detection result of the actinobacillus pleuropneumoniae is accurate, reliable, quick, flexible and stable without error, and the detection method is low in cost, simple in method, suitable for basic level, and brings great convenience for cultivation, disease prevention and disease treatment of pigs.

Description

technical field [0001] The invention belongs to the technical field of pig serum detection, in particular to a detection method for Actinobacillus pleuropneumoniae in pig serum. Background technique [0002] Porcine contagious pleuropneumonia is a respiratory infectious disease caused by Actinobacillus pleuropneumoniae (APP), characterized by acute hemorrhagic fibrinous pleurisy and chronic fibrinous necrotizing pneumonia. Since the disease was discovered in the United Kingdom in 1957, it has been widely prevalent in the world and has become one of the five major diseases that are internationally recognized as endangering the modern pig industry. Although the disease was discovered late in my country, it is more harmful to the breeding industry and has become one of the three common respiratory diseases in the sick pig industry in my country. [0003] There are many existing detection methods for Actinobacillus pleuropneumoniae, which can be roughly divided into three categ...

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Application Information

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IPC IPC(8): G01N33/569
Inventor 曹杰
Owner TIANJIN TIANZE LIVESTOCK & POULTRY BREEDING SPECIALIZED COOP
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