The invention relates to a method and kit for triple PCR detection of porcine infectious actinobacillus pleuropneumonia serum types 2, 3 and 6 and an application of the porcine infectious actinobacillus pleuropneumonia serum types 2, 3 and 6. The method comprises the steps: extracting DNA of a clinical porcine infectious pleuropneumonia strain; amplifying a PCR product, to be specific, adding a reaction solution consisting of a 10*PCR buffer solution, MgCl2, TaqDNA polymerase, dNTPs and three pairs of specific foreign matters types 2, 3 and 6 into a PCR reagent tube to extract DNA as a template, with the total volume of the reaction solution being 50 microliters; and finally detecting an amplified product. The method provided by the invention is strong in specificity, high in sensitivity, simple to operate, economic in labor and time, and capable of meeting the requirements for detecting multiple serum type genes at the same time, thus improving the accuracy and specificity in detection. According to the method and the kit provided by the invention, the method and the kit can be used for rapidly and conveniently detecting the porcine infectious actinobacillus pleuropneumonia serum types 2, 3 and 6, can be applied to bacterial identification, disease diagnosis, clinical vaccine screening and molecular epidemiology investigation and analysis, and have a wide market prospect and relatively high economic benefits.