91 results about "Porcine contagious pleuropneumonia" patented technology

The invention discloses a traditional Chinese medicine for treating respiratory diseases and promoting growth for swine and a preparation method thereof. The traditional Chinese medicine is composed of plantain herb, herba patriniae, hindu datura flower, perilla leaf, trichosanthes kirilowii maxim and gelsmium elegans. The traditional Chinese medicine of the invention can effectively prevent and treat livestock respiratory diseases, such as swine enzootic pneumonia, infectious pleuropneumonia of swine, porcine reproductive and respiratory syndrome (PRRS), swine plague, swine influenza and thelike; meanwhile, the traditional Chinese medicine of the invention can improve animal productivity and promote animal growth. The traditional Chinese medicine of the invention has wide raw material resources, is safe and environmentally-friendly and has the advantages of simple preparation technology, low production cost and the like.

The invention relates to the research field of biological diagnostic reagents, in particular to an ELISA (Enzyme-Linked Immunosorbent Assay) kit for detecting APP (Actinobacillus Pleuropneumoniae). The kit comprises a washing solution, a colorimetric solution, a stopping solution, a positive control specimen, a negative control specimen and an ELISA plate coating a first antibody as well as a second antibody for detection, wherein the first antibody is a monoclonal protein antibody of the APP rApxIVN, and the second antibody is a biotin-labeled monoclonal protein antibody of the APP rApxIVN. The invention can be applied to detecting APP-infected pig serum and has favorable importance and sensitivity.

The invention belongs to the technical field of domestic animal infectious disease, relating to the technical field of bacterial gene engineering, in particular to a construction, vaccine preparation and application of a reconstruction porcine actinobacillus pleuropneumoniae serum 1 type and porcine mycoplasmal pneumonia double-gene engineering strain. The invention is characterized in that a strain of reconstruction porcine actinobacillus pleuropneumoniae serum 1 type low virulent strain SLW05, the reconstruction strain is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO: M209068, porcine mycoplasmal pneumonia antigenicity P36 gene segment is inserted into the chromosome of porcine actinobacillus pleuropneumoniae serum 1 type low virulent strain SLW03, and the nucleotide sequence of the P36 gene segment is shown in sequence table SEQ ID NO: 1. the invention also discloses a bivalent gene engineering vaccine prepared by using the reconstruction strain and an application thereof.

The invention discloses a method for preparing an actinobacillus pleuropneumoniae (App) bacterial ghost and a method for preparing a subunit vaccine by loading a pasteurella antigen with the App bacterial ghost. A recombinant swine App bacterial ghost is prepared by controllable double-cracking technology and a pasteurella protection gene is introduced into an App bacterial ghost carrier, so that swine pleuropneumonia and a pasteurella bigeminal gene vaccine for preventing and treating swine pasteurellosis and swine pleuropneumonia are obtained. The preparation of the bacterial ghost carrier and the application of the bacterial ghost carrier to the prevention and treatment of important animal epidemic diseases are realized and a method is provided for the research of a multi-geminal gene vaccine at the same time. An animal experiment indicates that the protection rates of the bigeminal vaccine on infectious swine pleuropneumonia and pasteurellosis are up to 99 percent and 99.2 percent respectively.

The invention discloses a LAMP kit used for detecting actinobacillus pleuropneumoniae, and an application method thereof. The kit comprises 50mul of 8U / mul BstDNA polymerase, 125mul of a BstDNA polymerase buffer solution, 100mul of 0.1mol / l MgSO4, 100mul of 12.5mmol / l betaine, 100mul of 17.5mmol / l dNTP, and primers. As a result of multiple times of tests of practice, with the kit, trace amount of various serotypes of actinobacillus pleuropneumoniae can be detected specifically, sensitively, fast, and highly efficiently in a 63-64 DEG C constant-temperature water-bath pot. The method does not need complicated instrument and large amounts of professional skills, such that an urgent need currently of actinobacillus pleuropneumoniae field detection can be satisfied. The kit and the method can be used in field detections of import and export quarantine, food sanitation departments, animal husbandry farms, and the like. The kit and the method are especially suitable to be used by grassroots veterinary workers for carrying out wild and real-time detections of actinobacillus pleuropneumoniae.

The invention discloses a PCR diagnostic kit for porcine infectious pleuropneumonia, which comprises 400mu L of proteinase K with the concentration of 20mg / mL, 1000mu L of cracking solution, 1500mu L of TE buffer solution, 250mu L of PCR enzyme, 170mu L of ultra-pure water, 50mu L of MarkerDL2000, 40mu L of primer P1 and primer P2 which are mixed in the same volume and have the same concentration of 20mu M, 20mu L of negative control and 20mu L of positive control. By optimizing the PCR reaction conditions, the invention develops a PCR kit for detecting the actinobacillus of the porcine infectious pleuropneumonia; and by comparing the PCR detection result with the negative control and the positive control, detection conclusions can be obtained, thereby achieving the aim of quickly detecting whether a sample has porcine infectious pleuropneumonia or not. The invention has accurate detection result, quick and sensitive detection process, simple detection mode and good using effect.

The invention provides a preparation method of a triple inactivated vaccine for pigs. The method determines an antigen composition with excellent immunization effects by selection of the antigen. The prepared polyvalent vaccine has outstanding immunization effects. The prepared vaccine contains a PCP immunization protective antigen exotoxin (Aps), has cross immunization protection effects better than those of a whole cell inactivated vaccine, greatly reduces side reaction, and can simultaneously prevent haemophilus parasuis, swine streptococcosis and actinobacillus pleuropneumonia by combined immunization with inactivated haemophilus parasuis and streptococcus suis. Compared with haemophilus parasuis and streptococcus suis inactivated vaccines sold on the market, the triple inactivated vaccine has the same corresponding pathogen immune protection force. Compared with the actinobacillus pleuropneumonia inactivated vaccine sold on the market, the triple inactivated vaccine has a cross immunization protecting force on diseased pigs with different serotypes and realizes multiple protection purposes.

The invention discloses a mutant phage lysis gene E, a lysis plasmid vector containing the lysis gene and an application in preparation of bacterial ghost vaccines. The mutant phage lysis gene E (Eprom) disclosed by the invention is obtained after mutation of a promoter region of the lysis gene E of a phage phiX174, the E gene after mutation can turn the temperature of culture bacteria from the existing 28 DEG C to 37 DEG C, the mutant phage lysis gene E further has higher lysis efficiency, higher starting induction concentration and large-scale production capacity, and the culture lysis efficiency of a fermentation tank is as high as 99.99997%. After the Eprom is connected with pBV220, the high-efficient lysis plasmid vector pBV-Eprom can be obtained. The pBV-Eprom is transformed into actinobacillus pleuropneumoniae to induce the gene expression of the Eprom so as to get a bacterial ghost of the actinobacillus pleuropneumoniae. Porcine transmissible pleuropneumonia bacterial ghost vaccines disclosed by the invention has good safety and immune protection efficacy and can stimulate organisms to produce high-titer antibodies and further provide good cross immunoprotection against attacks of different serotypes of actinobacillus pleuropneumoniae virulent strains.

The invention discloses a gene chip for identification of seven swine disease pathogens and a detection method thereof. The gene chip can be used for detection of porcine actinobacillus pleuropneumoniae, haemophilus parasuis, mycoplasma hyopneumoniae, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, classical swine fever virus and porcine transmissible gastroenteritis virus. A PCR primer is designed by means of standard strain genome sequence analysis, cloning and sequencing analysis are carried out on a target gene, and specific probes are designed to construct the gene chip for detection, and optimization is performed to obtain a detection system. The invention aims to establish a method with the advantages of high sensitivity, strong specificity, time saving and labor saving, and easy observation of results to detect the seven important swine disease pathogens.

The invention discloses a nanoemulsion composition for treating mycoplasma pneumonia of livestock, which consists of raw materials including, by mass percent, from 0.1 to 8.0% of amyris oil, from 0.1 to 5.0% of vitex oil, from 0.1 to 3.0% of perilla oil, from 0.1 to 2.0% of bupleurum oil, from 0.01 to 0.1% of allicin, from 0.1 to 4.0% of tiamulin fumarate, from 19.0% to 31.0% of surfactant and the balance distilled water. The sum of the mass ratios of the ingredients is 100%. The nanoemulsion composition has effects of dilating the trachea of the livestock, suppressing cough reflex, increasing immunity of the organism, resisting inflammation and killing mycoplasma pneumoniae or other mixed infected causative agent, can be used for preventing and curing respiratory diseases including chronic respiratory diseases of the livestock, mycoplasma pneumoniae of swine, porcine contagious pleuropneumonia and the like, is fine in stability, is high in intestinal absorption utilization rate after being orally taken, can avoid liver first pass effect, and is remarkable in curative effect.

The invention discloses a gene chip for identification of six swine disease pathogens and a detection method thereof. The gene chip is used for detection of porcine actinobacillus pleuropneumoniae, haemophilus parasuis, mycoplasma hyopneumoniae, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and classical swine fever virus. A PCR primer is designed by means of standard strain genome sequence analysis, cloning and sequencing analysis are carried out on a target gene, and specific probes are designed to perform identification detection on porcine actinobacillus pleuropneumoniae, haemophilus parasuis, mycoplasma hyopneumoniae, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and classical swine fever virus 6 pathogens at the same time. The invention aims to establish the microarray chip with the advantages of high sensitivity, strong specificity, time saving and labor saving, and easy observation of results to identify and detect the important pathogens of porcine respiratory disease complex.

The invention discloses a traditional Chinese medicine for treating respiratory disease of livestock and a preparation method thereof. The traditional Chinese medicine comprises five traditional Chinese medicinal materials of herba violae, turkey corn, aster, herba ephedra and radix scutellariae. The traditional Chinese medicine can be effectively used for treating diseases of swine enzootic pneumonia, swine contagious pleuropneumonia, porcine reproductive and respiratory syndrome, swine plague, swine influenza, infectious bronchitis of chicken, infectious laryngotracheitis of chicken and thelike, and has the advantages of environment protection without toxic and side effects, simple preparation process, low production cost and the like.

The invention relates to a compound propolis composition for treatment of porcine contagious pleuropneumonia and a preparation method thereof, relating to the field of animal medicine. The preparation method comprises the following steps of: (1) performing ultrafine grinding of components in a formula, and screening with a 700-mesh sieve; (2) weighing 15-35 parts of propolis, 15-35 parts of astragalus mongholicus, 15-35 parts of tussilago, 15-35 parts of prepared pinellia tuber and 15-45 parts of jasmine by weight, and mixing for 5-10 minutes to prepare uniform mixture; (3) weighing 15-35 parts of fructus forsythiae, 15-40 parts of scutellaria baicalensis, 15-45 parts of cortex mori radicis, 15-45 parts of lindley eupatorium and 15-45 parts of pericarpium citri reticulatae by weight, and mixing for 5-10 minutes to prepare uniform mixture; and (4) mixing the mixture prepared in (2) and the mixture prepared in (3) for 10-15 minutes to prepare a mixture, thereby obtaining the compound propolis composition. The compound propolis composition has the beneficial effects of reasonable formula, simple preparation, no toxic or side effects, no pollution, no drug residue, no drug resistance, ultrafine grinding, high bioavailability and significant efficacy. Clinical applications prove that the composition has specially good effects on treatment of the porcine contagious pleuropneumonia.

The invention discloses a drug for curing contagious pleuropneumonia and a preparation method thereof. The drug comprises the following raw crude drugs: magnolia bark, vernonia cinerea, oviductus ranae, peppergrass, scutellaria root, goldthread root, humble plant, conyzae herba, rhodiola root, bamboo shavings, rice distillate, common reed rhizome, semen raphani, Japanese raspberry root, mezoneuron, small fruit fig aerial root, head stephania root, lycoperdales, castanea seguinii and thermopsis lanceolata. The drug has the benefits that the drug takes heat clearing, fire purging, antisepsis, anti-inflammation, lung nourishing, yin nourishing, and cough and asthma relieving as therapeutic principles, has a good curative effect on curing contagious pleuropneumonia, is quick to become effective, has small toxic and side effects, and is low in cost.

The invention discloses a preparation method of a test strip for rapidly diagnosing hematoxin ApxI arranged outside porcine contagious pleuropneumonia actinobacillus, and relates to the technical field of rapid biological detection. The test strip comprises an analyzing film (5) and a gold-labelled antibody combining pad (1), wherein a detection line 1 (T1) and a detection line 2 (T2) are made from an actinobacillus-resisting exotoxin ApxI / ApxIV monoclonal antibody arranged on the analyzing film (5), a quality control line (C) is made from a goat anti rabbit IgG, and the gold-labelled antibody combining pad (1) is a fusion protein anti rabbit polyclonal antibody of p380-ApxI-ApxIV. The preparation method provided by the invention solves the problems that the inspection speed is slow, the sensitivity is low, the operation is complicated and the like in the prior art.

The invention discloses a feed for treating porcine contagious pleuropneumonia and a preparation method of the feed. The feed comprises the following raw materials: rice, pearl barley, wheat bran, lily powder, whey powder, plasma protein powder and medicaments for treating the porcine contagious pleuropneumonia. The feed has the beneficial effects that the feed contains the medicaments for treating the porcine contagious pleuropneumonia, and the medicaments have therapeutic principles of clearing away heat, purging pathogenic fire, resisting bacteria, diminishing inflammation, nourishing lungs and yin and relieving cough and asthma, are interacted with the other raw materials to play a combined role and have the advantages of good treat effect, quick effectiveness and low toxic and side effects and cost when being used for treating the porcine contagious pleuropneumonia.

The invention relates to the technical field of veterinary drugs, in particular to a vaccine of swine fever-porcine contagious pleuropneumoniae bivalent subunit and a preparation method and applications thereof. The vaccine comprises classical swine fever virus E2 protein, serum type-7 actinobacillus pleuropneumoniae toxin protein ApxII, serum type-1 actinobacillus pleuropneumoniae outer membraneprotein Oml and vaccine adjuvant. The vaccine is obtained by mixing and emulsifying the proteins and the vaccine adjuvant in equal volume. The vaccine can simultaneously prevent swine fever and porcine contagious pleuropneumoniae caused by serum type-1 and type-7 porcine actinobacillus pleuropneumoniae, and has good effect and low cost.

The invention discloses a resistance marker-free Apx I C gene deleted mutant strain of porcine infectious actinobacillus pleuropneumonia. The number of the vaccine candidate strain is No. SW1delta I C. The strain is preserved in China Center for Type Culture Collection (CCTCC) on August 31, 2011, with a preservation number of CCTCC M 201130. The activating gene C of the toxin Apx I is deleted by means of genetic engineering, and the size of the deleted fragment is 475bp. The gene deleted strain constructed in the invention has genetic stability, and cannot reverse to virulence. In the invention, the gene deleted strain is also utilized to prepare a gene deleted attenuated vaccine. Meanwhile, immune efficacy tests of laboratory rats and piglets prove that the vaccine can induce good immune protection.

The invention discloses a genetic engineering subunit mixed vaccine as well as a preparation method and an application thereof and belongs to the field of research of veterinary vaccines. Three recombinant proteins rApxIA, rApxIIA and rOMPD are expressed abundantly with a genetic engineering method and have better antigenicity. The rOMPD and rApxIA antibody levels of the mixed vaccine are higher than those during separate immunization of three proteins, the antibody level of rApxIIA and the antibody level of the mixed vaccine are remarkably higher than that of a control group, and the result proves that humoral immunity reaction induced by the recombined subunit mixed vaccine and rApxIA and rOMPD antibodies play an important role in cross immunization protection of mice. After inoculation, the genetic engineering subunit mixed vaccine is safe and harmless to an immune animal, is a novel vaccine with wide prospect, provides material reserve and technical support for control of porcine contagious pleuropneumonia in china and has great significance.

The invention discloses a medicinal composition, which comprises the following components in parts by weight: 5 to 15 parts of amoxicillin, 1 to 5 parts of clavulanate potassium, and 1 to 5 parts of ambroxol hydrochloride. The invention discloses an application of the medicinal composition in preparing medicaments for treating animal respiratory infectious diseases, in particular for preparing porcine infectious pleuropneumonia and haemophilusparasuis. The invention also discloses a preparation method of compound amoxicillin soluble powder. The compound amoxicillin soluble powder comprises the following components in parts by weight: 5 to 15 parts of amoxicillin, 1 to 5 parts of clavulanate potassium, 1 to 5 parts of ambroxol hydrochloride, and 75 to 93 parts of glucosum anhydricum. The raw materials are uniformly mixed according to an equivalent incremental principle to obtain the compound amoxicillin soluble powder. The medicinal composition disclosed by the invention is reasonable and scientific in prescription, the bioavailability of amoxicillin can be increased compared with an amoxicillin preparation, the drug resistance is reduced, and the curative effect is improved.

The invention discloses a streptococcus suis disease-haemophilus parasuis disease-porcine infectious pleuropneumonia triple subunit vaccine and a preparation method thereof. The triple subunit vaccine contains streptococcus suis antigen proteins MRP, SLY and EF, haemophilus parasuis antigen proteins AfuA, OppA2, CdtB and OppA, and actinobacillus pleuropneumoniae antigen proteins ApxI, ApxII and OMP. Experiments prove that the vaccine can stimulate strong immune response of mice, and has a good cross protection effect on streptococcus suis, haemophilus parasuis and actinobacillus pleuropneumoniae challenge mice with different serotypes, which fully shows that the triple subunit vaccine prepared in the invention has a good protection effect. Therefore, the invention lays a solid foundation for developing an efficient, broad-spectrum and cheap streptococcus suis disease-haemophilus parasuis disease-porcine infectious pleuropneumonia vaccine, and provides an effective technical means for preventing and treating streptococcus suis disease, haemophilus parasuis disease and porcine infectious pleuropneumonia.

The invention belongs to the technical field of clinical immunology, and discloses a monoclonal antibody of actinobacillus pleuropneumoniae rApxIVAN and application thereof. Partial fragments of ApxIVgene are effectively intercepted and mutated, and the obtained genes can be efficiently expressed in escherichia coli and can also be expressed in supernate. Expressed and purified rApxIVAN are usedas an antigen to prepare the monoclonal antibody, an indirect competitive ELISA antibody detection method for actinobacillus pleuropneumoniae is established, and the specificity is improved. The method of the invention can not only used to detect and monitor all APP serotype antibodies, but also distinguish wild virus infection and existing vaccine immunity, which provides a powerful tool for effect evaluation and purification of swine infectious pleuropneumonia vaccines.

The invention discloses an actinobacillus pleuropneumoniae (APP) LAMP diagnostic kit. According to the invention, a complete LAMP system is constructed; an LAMP amplification method can use a water bath kettle or a heat preservation cup for amplification, the results are visible to naked eyes, the operation is relatively simple, basic on-site quarantine is particularly facilitated, and the sensitivity is high. The kit constructed by a primer designed by the invention can detect APP 1-15 types and has the sensitivity of 0.3 pg / L which is 100 times higher than that of PCR; thus, the established LAMP diagnostic method can perform rapid field detection in pig farms, and is accurate in result and convenient to use.

The invention provides a primer composition for assistant identification of porcine infectious pleuropneumonia ApxI toxin and application thereof, and belongs to the technical field of biology. A specific primer pair provided by the invention consists of single stranded DNA (deoxyribonucleic acid) molecules shown as a sequence table 1 to 4. The specific primer pair provided by the invention can beused for identifying porcine infectious pleuropneumonia actinobacillus toxin. The primer composition provided by the invention is combined with double PCR (polymerase chain reaction), so that the ApxI A and Apx IV A toxin can be simultaneously identified; the characteristics of high sensitivity, high specificity, short time, low cost and the like are realized; the primer composition can be usedfor clinical sample detection; the operation is simple; the popularization is easy; the basic level operation and the application are convenient.

The invention relates to a bivalent vaccine composition resisting type-2 porcine circovirus and porcine infectious pleuropneumonia infection and a preparation method. The bivalent vaccine composition comprises a type-2 porcine circovirus antigen and an actinobacillus pleuropneumoniae antigen; the immune procedure is simple; the type-2 porcine circovirus related diseases and porcine infectious pleuropneumonia can be effectively prevented at the same time. Compared with a single vaccine, the bivalent vaccine composition does not generate an immune interference phenomenon while the immune effect is not reduced; moreover, the actinobacillus pleuropneumoniae can enhance the immune effect of the type-2 porcine circovirus antigen. The preparation process of the bivalent vaccine composition is simple, the immune cost is low, and the practicability is high.

The invention discloses a porcine actinobacillus pleuropeumoniae ClpP protease gene-deleted strain containing no resistance marker, and a construction method thereof. The invention belongs to the technical field of bacteria genetic engineering. According to the invention, a recombinant strain APPdeltaclpP of actinobacillus pleuropeumoniae is a strain obtained through coding gene deactivation upon ClpP protease in actinobacillus pleuropeumoniae with an oriented homologous recombination technology, and the expression of the ClpP protease is damaged. With the technology provided by the invention, the virulence of the mutant strain is lower than a parent strain, and the mutant strain is safe to animals. Therefore, an important basis is provided for the modification upon porcine contagious pleuropneumonia vaccines and the development of corresponding differential diagnosis reagents. The strain and the method provided by the invention have important significances in promoting the eradication and purification of porcine contagious pleuropneumonia all around the world.

The invention provides a vaccine composition for preventing and treating respiratory diseases secondary to atrophic rhinitis, which comprises: an immunization amount of antigens of pig actinobacillus pleuropneumoniae; an immunization amount of antigens of pasteurella multocida; an immunization amount of antigens of brodetella bronchiseptica; and adjuvants. The vaccine composition has the advantages of high safety performance, good immunogenicity, long immunity period, low cost, and the like, can reduce cost, decrease immunization labor amount, and reduce animal stress.

The invention discloses a porcine infectious actinobacillus pleuropneumoniae subunit vaccine, and belongs to the field of veterinary vaccines. The porcine infectious actinobacillus pleuropneumoniae subunit vaccine comprises protein 1-3 combinations with amino acid sequences shown as SEQ ID NO. 1-3 respectively. Truncated proteins selected in the antigen protein combinations are highly homologous in all serotypes of APP, and the subunit vaccine prepared from the truncated proteins can provide cross protection for porcine infectious actinobacillus pleuropneumoniae of different serotypes; and the truncated proteins are expressed in a soluble form, and are easy to produce and low in cost.

The invention discloses a strain of Lactobacillus plantarum ZN-3 and its application. The Lactobacillus plantarum ZN-3 provided by the invention is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is: CCTCC NO: M 2017286. The Lactobacillus plantarum ZN-3 provided by the present invention has the characteristics of acid resistance and bile salt resistance, can colonize the intestinal tract through the stomach, and has antibacterial effect on animal-derived pathogenic bacteria, including Gram-negative bacteria: large intestine bacillus, Salmonella, Pastobacterium multocida, Erysipelothrix suis, Haemophilus parasuis, porcine infectious pleuropneumonia, also includes Gram-positive bacteria: Streptococcus suis, Staphylococcus aureus, Staphylococcus epidermidis. Compared with antibiotics, it has a good antibacterial effect on pathogenic bacteria with strong drug resistance.

The invention relates to a method and kit for triple PCR detection of porcine infectious actinobacillus pleuropneumonia serum types 2, 3 and 6 and an application of the porcine infectious actinobacillus pleuropneumonia serum types 2, 3 and 6. The method comprises the steps: extracting DNA of a clinical porcine infectious pleuropneumonia strain; amplifying a PCR product, to be specific, adding a reaction solution consisting of a 10*PCR buffer solution, MgCl2, TaqDNA polymerase, dNTPs and three pairs of specific foreign matters types 2, 3 and 6 into a PCR reagent tube to extract DNA as a template, with the total volume of the reaction solution being 50 microliters; and finally detecting an amplified product. The method provided by the invention is strong in specificity, high in sensitivity, simple to operate, economic in labor and time, and capable of meeting the requirements for detecting multiple serum type genes at the same time, thus improving the accuracy and specificity in detection. According to the method and the kit provided by the invention, the method and the kit can be used for rapidly and conveniently detecting the porcine infectious actinobacillus pleuropneumonia serum types 2, 3 and 6, can be applied to bacterial identification, disease diagnosis, clinical vaccine screening and molecular epidemiology investigation and analysis, and have a wide market prospect and relatively high economic benefits.