39 results about "Long evans rats" patented technology

The present invention relates to the development of a pharmacological non-human animal model that associates memory loss to histopathological features found in the brain of a subject having Alzheimer's Disease. In one embodiment, a four-week continuous infusion of a Fe2+, Aβ42 and buthionine sulfoximine (FAB) solution in the left ventricle of young adult Long-Evans rats induced memory impairment accompanied by increased hyperphosphorylated Tau protein levels in cerebrospinal fluid. Brains from treated animals displayed neuritic plaques, tangles, neuronal loss, astrogliosis and microgliosis in hippocampus and cortex. The present invention may be utilized in evaluating preventive, therapeutic and diagnostic means for neurologic diseases.

A method for inhibiting formation of adhesions following abdominal surgery which involves application of an anti-static fatty acid ethoxylated amide (Cocamide DEA) in a matrix that is placed in the peritoneal cavity at the conclusion of an abdominal surgery and which releases this anti-adhesive chemical over a predetermined time in a range up to seven days. Tests conducted on laboratory rats established that the method reduced the incidence of adhesions from 100 percent (100%) in a test model to near zero in the majority of treated animals. In an alternative embodiment, andrographalide was delivered via a pump with similar results. In still another embodiment, an effective amount of 50% phosphatidylchorene and propylene glycol was delivered, via a pump, into the abdominal cavity, again with similar results.

A method for inhibiting formation of adhesions following abdominal surgery which involves application of an anti-static fatty acid ethoxylated amide (Cocamide DEA) in a matrix that is placed in the peritoneal cavity at the conclusion of an abdominal surgery and which releases this anti-adhesive chemical over a predetermined time in a range from about five to seven days. Tests conducted on laboratory rats established that the method reduced the incidences of adhesions from 100 percent (100%) in a test model to near zero percent (0%) in the treated animals. In an alternative embodiment, Andrographalide was delivered through a drug pump with similar results. In still another embodiment, an effective amount of Rapamune was delivered, via a pump, into the abdominal cavity, again with similar results.

The invention discloses application of whey protein peptide in the preparation of a medicament, a health-care food or a food for accelerating the growth and development and increasing bone density. The whey protein peptide has the characteristics of easy absorption, quick absorption, low viscosity, good water solubility and the like, can promote the increasing of body length and weight of a laboratory rat and increase the bone density of near sections of thigh bones and bone mineral content of the rat, and has the functions of accelerating growth and development and increasing bone density. The whey protein peptide has important scientific value and economic benefit when applied to the preparation of the medicament, the health-care food or the food for accelerating growth and development and increasing bone density and provides a new approach for accelerating the growth and development of an organism through diet.

The invention relates to antrodia cinnamomea extract liquor for reducing blood alcohol concentration. The antrodia cinnamomea extract liquor comprises maleic imide compounds extracted from oil in a cracked cell wall of antrodia cinnamomea mycelium as an effective ingredient, and mixed with a certain amount of edible oil to form a solution form, wherein the edible oil and the effective ingredient are mixed according to the weight ratio of (9:1) to (7:3). Therefore, the activity of alcohol dehydrogenase in the body of a small laboratory rat fed by the antrodia cinnamomea extract liquor disclosed by the invention is obviously increased by about 50-60% relatively, and the alcohol concentration in the blood is also obviously reduced by about 30-40% relatively.

The invention discloses a resistance marker-free Apx I C gene deleted mutant strain of porcine infectious actinobacillus pleuropneumonia. The number of the vaccine candidate strain is No. SW1delta I C. The strain is preserved in China Center for Type Culture Collection (CCTCC) on August 31, 2011, with a preservation number of CCTCC M 201130. The activating gene C of the toxin Apx I is deleted by means of genetic engineering, and the size of the deleted fragment is 475bp. The gene deleted strain constructed in the invention has genetic stability, and cannot reverse to virulence. In the invention, the gene deleted strain is also utilized to prepare a gene deleted attenuated vaccine. Meanwhile, immune efficacy tests of laboratory rats and piglets prove that the vaccine can induce good immune protection.

The invention relates to the technical field of animal blood sampling equipment and particularly discloses a laboratory rat tail cutting blood sampling device. The laboratory rat tail cutting blood sampling device comprises a latex cover, a detachable glass tube, a conical groove, an air suction hole, a vacuum surrounding wall, an exhaust hole, a bottom adjusting device and a passing hole. According to the laboratory rat tail cutting blood sampling device, the vacuum principle is mainly used, air in a bottle is sucked, so that negative pressure is formed in the bottle, blood can flow out soon under the action of negative pressure, and thus the purpose of carrying out multiple times and a large amount of blood sampling on a laboratory rat is achieved; the blood sampling device is provided with the vacuum surrounding wall so that blood back jetting due to air leakage can be avoided, the height of the bottom of the blood sampling device can be adjusted according to the length of different tails through the bottom adjustor, and the blood sampling device is easy and convenient to use and capable of saving time.

The invention belongs to the technical field of laboratory mouse trapping devices, and specifically relates to a telescopic length metal pliers, including two inlays on the left and right. A threaded hole, a fixing screw is provided in the first threaded hole, a telescopic rod is provided under the sleeve, the upper part of the telescopic rod is set in the sleeve, and a first blind hole matching the first threaded hole is provided on the upper part of the telescopic rod. There is a first groove on the edge of the upper end surface of the rod, the length of the first groove is smaller than the length of the telescopic rod, and a second threaded hole is radially provided on the lower part of the telescopic rod, and a ball plunger is arranged in the second threaded hole. A bushing is provided under the rod, a second blind hole matching the third threaded hole is provided on the upper part of the boss, a second through hole is provided on the front end of the pliers arm, and the two pliers arms are hinged together through the second through hole. The lower part of the arm is provided with a pincer head. The purpose of the utility model is to solve the problem of inconvenient adjustment of the length of the pincer arm caused by the structural defect of the existing mousetrap.

A garbage deodorant is prepared from substances in parts by mass as follows: 10-20 parts of a plant hydrated extract, 30-50 parts of edible mushroom waste and 5-10 parts of a microbial agent. The garbage deodorant has an effective application in the aspect of deodorization of stale refuse or domestic organic garbage. The plant deodorant product is toxic-free and harmless and has no irritation. Experiments through laboratory rats and little white rabbits prove that the deodorant has no transoral toxicity or irritation to skin. The efficient compound microbial deodorant for a garbage dump is lowin cost, high in efficiency, wide in application and very practical in specific production.

The invention discloses a bone biopsy needle and provides an implant and a method for establishing a bacterial biofilm infected animal model. The implant is a hollow tubular object; one end of the implant is aculeated and used for piercing the skin of an animal to be molded. The invention also provides a method for establishing the bacterial biofilm infected animal model by using the implant. The implant and the method for establishing the bacterial biofilm infected animal model do not need open operations, are simple and convenient to operate, not only are suitable for larger animals like rabbit, guinea pig and rat, but also can be used for laboratory rats. Moreover, the wound surfaces are small, and the animals can recover quickly. Therefore, the bad influences of anthropogenic factors like operative procedures and the like on the experimental animals can be effectively limited; and the condition control during the establishing of models is improved.

The invention discloses a quick-disinfection temporary cage for experimental mice, which comprises a temporary cage body and a cage door. The upper and lower sides of the front side wall of the temporary cage body are symmetrically provided with chutes, and the temporary cage body The interior of the cage is provided with a rat-repelling board, one side of the temporary cage body is equidistantly distributed with air nozzles from top to bottom, and the top of the temporary cage body is provided with a shunt pipe. In the present invention, sprinklers are equidistantly distributed on the top of the temporary cage, and through these nozzles, the disinfectant inside the liquid storage bin can be quickly and evenly sprayed into the cage, thereby completing the rapid and automatic disinfection of the temporary cage. The air nozzles distributed equidistantly from top to bottom on one side can blow the wind generated by the fan to the sterilized holding cage in the form of diffusion, thus effectively improving the air-drying efficiency. Through the combined use of these structures, not only the Automatic disinfection of temporary cages, while the disinfection process is efficient and fast, and can be put into use soon.

The invention belongs to the technical field of medical devices and particularly relates to a laboratory rat dissection device and a use method thereof. The laboratory rat dissection device comprisesa dissection table, a dissection cushion, fixing parts and binding parts, wherein the dissection cushion is detachably mounted on the dissection table; a plurality of inserting holes are uniformly distributed in the dissection cushion; and the lower parts of the fixing parts and the binding parts are detachably inserted into the inserting holes. The dissection table and the dissection cushion aredetachably connected, so that a researcher conveniently selects the proper dissection cushion according the size of body of a laboratory rat and the dissection cushion can be conveniently dismounted and disinfected. The use method of the laboratory rat dissection device is simple and convenient to operate and facilitates the dissection work of the laboratory rat by a single researcher; and all main components of the dissection device are detachably connected and mounted and are convenient to clean and disinfect.

A nano-ceramic scalpel is free of magnetism and static electricity, long in service life and high in precision. The biocompatibility with human tissue is good, acidemia infiltration can be avoided, disinfection can be achieved easily, no stimulation and antibody responses exist, wound infection by metal diffusate cannot happen, incisions can be healed quickly, no obvious scars are left after operations, and the nano-ceramic scalpel is particularly suitable for the microsurgery, plastic surgery and the like. However, nano ceramic is extremely high in hardness and low in toughness, and thus manufacturing and processing are quite difficult. The invention provides an ELID sharpening technology and equipment which are used for manufacturing the nano-ceramic scalpel. According to the nano-ceramic scalpel as shown in Figure 1, the ELID sharpening technology and the self-designed process equipment are used for successfully grinding the two side faces of the nano-ceramic scalpel, the surface roughness reaches 6nm, and the cutting edge radius of the nano-ceramic scalpel reaches 200nm. The manufactured nano-ceramic scalpel is high in wound healing speed, free of obvious scars after healing and good in effect in clinical incision experiments of laboratory rats.

The invention provides a feed for laboratory rats and mice. The feed is obtained by feeding a feed into capsules. The feed is mainly applied in the following ways: 1. processing special feeds which are difficultly processed into standard granulated feeds such as high-fat diets and special-formula feeds; 2. processing feeds added with certain unstable or special active substances to prevent inactivation of active substances during processing of conventional feeds; 3. feeding animals with certain special substances or medicaments in the form of feed for a long time in animal experiments to ensure quantitative taking of animals and avoid hurting experimental animals caused by long-time gavage or injection; and (4) performing microencapsulation on certain additives / medicaments which have special smell or are unstable and tend to be inactivated in combination with a microencapsulation technology and feeding into feeds for feeding to ensure the palatability and the quantitative taking of feeds.

The invention relates to the technical field of laboratory animal feed for biological medicine and provides feed for a sterile laboratory rat. The feed comprises components as follows: 14-28 IU / g vitamin A, 1.5-4.3 IU / g vitamin D, 120-240 IU / g vitamin E, 5-20 mg / kg vitamin K3, 13-26 mg / kg vitamin B1, 12-24 mg / kg vitamin B2, 12-24 mg / kg vitamin B6, 60-120 mg / kg nicotinic acid, 24-48 mg / kg pantothenic acid, 4-8 mg / kg folic acid, 0.2-0.4 mg / kg biotin and 0.03-0.06 mg / kg vitamin B12. A method for preparing the feed comprises steps as follows: detection of nutritional ingredients in base grains, addition of nutrient substances and granulation. The feed for the sterile laboratory rat is beneficial to growth of the sterile laboratory rat and rapid healing of wounds.

A system and method for measuring a magnetocardiogram (MCG) in order to measure a weak magnetic field generated from the heart of a small animal such as a laboratory rat are provided. The system includes a case, a SQUID sensor located and fixed inside the case to detect magnetism, a platform arranged near the SQUID sensor inside the case, the small animal being placed on the platform, a linear station to which the platform is fixed to move a location of the platform, and a control unit configured to control operations of the SQUID sensor and the linear station and measure the MCG of the small animal using intensities of the magnetism detected by the SQUID sensor.

The invention relates to a novel positioning evaluation anti-counterflow device for a laboratory rat oral trachea cannula. The novel positioning evaluation anti-counterflow device comprises the cannula, an adapter, a tee joint, an air guide tube, a switch and a bubble barrel. The cannula is connected with the front end of the adapter, the rear end of the adapter is connected with a first port of the tee joint, a second port of the tee joint is connected with a respirator, the front end of the air guide tube is connected with a third port of the tee joint, and the rear end of the air guide tube is communicated with the bubble barrel. An airflow channel with the round cross section and the trapezoidal longitudinal section is formed in the bubble barrel, the port, with the small hole diameter, of the airflow channel is communicated with the air guide tube, the port, with the large hole diameter, of the airflow channel serves as a bubble outlet, and the switch is installed on the air guide tube. It can be guaranteed that the trachea cannula is located in the trachea lumen of a rat and kept smooth, bubbles escaped from the bubble barrel in the expiration period of the rat are easy to observe, and liquid can be prevented from counterflow from the bubble barrel and the air guide tube in the inspiration period of the rat.

The invention discloses a method for improving mating ability of male Wistar rats, and belongs to the technical field of experimental animal production. The method is characterized in that four traditional Chinese medicine extracts of herba cistanche, herba cynomorii, herba epimedii and fructus lycii are combined according to the concentration ratio of 0.5 mg / mL, 1.0 mg / mL, 0.6 mg / mL and 1.4 mg / mL, the male Wistar rats are subjected to intragastric administration every day, the mating ability of the male Wistar rats in a short period is obviously enhanced, the rat catching and climbing times are effectively increased, the catching and climbing incubation period is obviously reduced, the breeding period is greatly shortened, the application values of low cost, short time consumption, simple operation and the like in the aspects of laboratory rat breeding, breeding system establishment and the like are achieved, and the method has practical reference basis and wide application prospect in the aspects of breeding protection of endangered mammals, treatment of erectile dysfunction of male animals, development of new drugs and the like.

A method for inhibiting formation of adhesions following abdominal surgery which involves application of an anti-static fatty acid ethoxylated amide (Cocamide DEA) in a matrix that is placed in the peritoneal cavity at the conclusion of an abdominal surgery and which releases this anti-adhesive chemical over a predetermined time in a range up to seven days. Tests conducted on laboratory rats established that the method reduced the incidence of adhesions from 100 percent (100%) in a test model to near zero in the majority of treated animals. In an alternative embodiment, andrographalide was delivered via a pump with similar results. In still another embodiment, an effective amount of 50% phosphatidylchorene and propylene glycol was delivered, via a pump, into the abdominal cavity, again with similar results.

The invention relates to an operation method of a laboratory rat weight, heart rhythm and body temperature monitoring device and provides the laboratory rat weight, heart rhythm and body temperature monitoring device. The method comprises the following operation steps that S01, the power source of the laboratory rat experiment monitoring device is switched on, limit screws are rotated, and a limit net is adjusted to the highest position; S02, a hemispherical transparent cover is opened, and a laboratory rat is put on a round base; S03, an injection cylinder is filled with medicine liquid; S04, the laboratory rat is covered with the hemispherical transparent cover, and the limit net is pressed down; S05, the position of a clamping support is adjusted, and a needle-type sprayer is aligned with the position, where injection is about to be conducted, of the tail of the laboratory rat; S06, the position of a clamp of the tail of the laboratory rat on a track is adjusted, and then the tail of the laboratory rat is clamped; S07, an OK key is pressed down, a display screen is observed, and data is recorded at the time interval of T. The method can guide a user to operate the monitoring device, and work efficiency and the reliability of the device monitoring data can be greatly improved.

The invention relates to a novel positioning evaluation anti-counterflow device for a laboratory rat oral trachea cannula. The novel positioning evaluation anti-counterflow device comprises the cannula, an adapter, a tee joint, an air guide tube, a switch and a bubble barrel. The cannula is connected with the front end of the adapter, the rear end of the adapter is connected with a first port of the tee joint, a second port of the tee joint is connected with a respirator, the front end of the air guide tube is connected with a third port of the tee joint, and the rear end of the air guide tube is communicated with the bubble barrel. An airflow channel with the round cross section and the trapezoidal longitudinal section is formed in the bubble barrel, the port, with the small hole diameter, of the airflow channel is communicated with the air guide tube, the port, with the large hole diameter, of the airflow channel serves as a bubble outlet, and the switch is installed on the air guide tube. It can be guaranteed that the trachea cannula is located in the trachea lumen of a rat and kept smooth, bubbles escaped from the bubble barrel in the expiration period of the rat are easy to observe, and liquid can be prevented from counterflow from the bubble barrel and the air guide tube in the inspiration period of the rat.

The present invention relates to the development of a pharmacological non-human animal model that associates memory loss to histopathological features found in the brain of a subject having Alzheimer's Disease. In one embodiment, a four-week continuous infusion of a Fe2+, Aβ42 and buthionine sulfoximine (FAB) solution in the left ventricle of young adult Long-Evans rats induced memory impairment accompanied by increased hyperphosphorylated Tau protein levels in cerebrospinal fluid. Brains from treated animals displayed neuritic plaques, tangles, neuronal loss, astrogliosis and microgliosis in hippocampus and cortex. The present invention may be utilized in evaluating preventive, therapeutic and diagnostic means for neurologic diseases.

The invention discloses a conical head sleeve for a laboratory rat in the field of a laboratory appliance. The conical head sleeve comprises a framework, wherein a leather sleeve covers the outside of the framework; the framework comprises a left half frame, a right half frame and a rotating shaft; the left half frame is movably connected with the right half frame through the rotating shaft; a fixed frame is respectively arranged on the outer side edge of each of the left half frame and the right half frame in the length direction; the two fixed frames are in symmetrical arrangement by using the rotating shaft as an axis; a flexible connecting rod is connected onto each of the two fixed frames; a fixed ear stud is movably connected onto each of the two flexible connecting rods; the two fixed ear studs are in a collineation state in the length direction; one end of each fixed ear stud is made into a sharp end inserted into the rat ear; a finger ring used for fixing a finger of an experiment person is fixedly connected onto the rotating shaft; at least one fixing rack is fixedly connected onto the outer side in the length direction of the left half frame; a fixing buckle matched with the fixing rack in a buckling and connecting way is connected onto the right half frame. Through the blocking by the leather sleeve, the stab wound probability of the finger of the experiment person by the teeth of the laboratory rat is reduced; the finger of the experiment person can also conveniently realize the tight holding according to the concrete shape of the head of the laboratory rat.

The invention discloses an application of esculetin (6,7-dihydroxy coumarin), wherein the molecular formula of the esculetin is C9H6O4, and the chemical structural formula of the esculetin is described in the specification. The esculetin is used for protecting the brain of a rat from ischemia reperfusion damage. In the ischemia reperfusion damage model of the median of the rat brain, the injection of the esculetin for a laboratory rat can obviously reduce the cerebral infarction area and the neurobehavioral capability of the rat is improved.

The invention relates to a preparation method, a product and an application of horseshoe crab peptide cream. The preparation method of the horseshoe crab peptide cream comprises the steps as follows:1) meat liquids and eggs of horseshow crabs are collected and pulped by a colloid mill, and fine pulp is obtained; 2) animal protease and flavor enzyme are added to the fine pulp for hydrolysis, heating for enzyme deactivation is conducted after hydrolysis ends, and an enzymatic hydrolysate is obtained; and 3) the enzymatic hydrolysate is subjected to vacuum concentration and the horseshoe crab peptide cream is obtained. The horseshoe crab peptide cream prepared with the preparation method can be used as an attractant, the ingestion desire of laboratory rats can be increased, the food intake of the laboratory rats can be guaranteed, and the molding quality of animal models can be improved.

The invention relates to a laboratory rat fixing device, so as to solve the problem of lack of a safe, effective, fast and convenient fixing mode in the existing laboratory rat injection process. Thelaboratory rat fixing device includes a laboratory table, on which various rotating ring groups are vertically arranged in the axial direction. The rotating ring group is formed by sequentially nesting and rotatably connecting at least two eccentric rings with different sizes. The upper end faces of the at least two eccentric rings are respectively flush with the upper end face of the laboratory table. The eccentric ring at the innermost layer of the rotating ring group is provided with a rotary table which is coaxially and rotatably connected with the inner annular surface of the eccentric ring. The rotary table is provided with two slot holes vertically penetrating the rotary table. The device of the invention also includes multiple cable ties in one-to-one correspondence with the rotating ring groups and multiple counterweight blocks which are arranged below the rotating ring groups and are in one-to-one correspondence with the cable ties. Two ends of the cable tie respectively penetrate through the two slot holes from top to bottom and are arranged below the rotating ring group and connected with the counterweight block. Brake discs which are arranged below the rotating ring groups and are in one-to-one correspondence with the rotating ring groups are arranged on the laboratory table. The brake discs are driven through a driving mechanism to move vertically on the laboratory table.

The present invention relates to the technical field of experimental rat fixation devices, in particular to an adjustable fixed platform for acupuncture and moxibustion of experimental rats. There are arc-shaped through holes on both sides of the mouse trough, body stoppers are arranged between the two arc-shaped through holes, and oblique through holes are opened at the four corners of the top surface of the bearing plate. A binding mechanism is provided on the top of the oblique through hole. In the present invention, the two sides of the body of the experimental rat are clamped by two arc-shaped plates, and the two adjacent restraint blocks are unfolded by rotation. The legs are clamped and fixed, and the neck of the experimental mouse is fixed through the cooperation of the child Velcro and the mother Velcro, so as to realize the all-round limit fixation of the experimental mouse, facilitate acupoint acupuncture, and improve the effect of acupuncture and moxibustion.

The invention discloses an evaluation method of rat models suffering from endometriosis, kidney deficiency and blood stasis syndromes. The evaluation method comprises the following steps: normally feeding experimental rats for one week, then mating with 3-month-old SD male rats according to a ratio of 2:1, and drenching with mifepristone once on the 12 day of pregnancy to cause abortion of pregnant rats, wherein hydroxyurea and the mifepristone are ground into powder and are dissolved in distilled water for drenching; from the next day after stopping of vaginal bleeding of the aborted rats, hitting with a wooden stick to force the rats to swim constantly until a fatigued state, fishing out the experimental rats after swimming, and exposing the experimental rats in air for naturally airing; anesthetizing the rats under an aseptic condition, laparotomizing for shearing intimal tissue blocks, reversely aligning epithelial layers to body walls, closing abdomens, randomly taking 3 ratsafter 4 weeks, laparotomizing again for cutting transplants for inspection, and verifying that the rats have survived. According to the evaluation method, modeling of experimental animals suffering from traditional Chinese medicinal syndromes is easy to control, an accurate and effective way of identifying syndrome characteristics is increased, and a great convenience is brought to clinical diagnosis, therapeutic effect evaluation, novel medicine development and international communication of the traditional Chinese medicine.