The invention discloses a method for improving mating ability of male Wistar rats, and belongs to the technical field of experimental animal production. The method is characterized in that four traditional Chinese medicine extracts of herba cistanche, herba cynomorii, herba epimedii and fructus lycii are combined according to the concentration ratio of 0.5 mg/mL, 1.0 mg/mL, 0.6 mg/mL and 1.4 mg/mL, the male Wistar rats are subjected to intragastric administration every day, the mating ability of the male Wistar rats in a short period is obviously enhanced, the rat catching and climbing times are effectively increased, the catching and climbing incubation period is obviously reduced, the breeding period is greatly shortened, the application values of low cost, short time consumption, simple operation and the like in the aspects of laboratory rat breeding, breeding system establishment and the like are achieved, and the method has practical reference basis and wide application prospect in the aspects of breeding protection of endangered mammals, treatment of erectile dysfunction of male animals, development of new drugs and the like.