The invention discloses a formula of grape medicinal liquor and a preparation method thereof, and belongs to the technical field of food processing. According to the formula, the grape medicinal liquor is prepared from the following components in weight by part: 40 to 50 parts of grapes, 3 to 4 parts of medlar, 9 to 14 parts of brown sugar, 5 to 7 parts of honey, 1 to 5 parts of mulberry, 2 to 3 parts of liquoric root and 30 to 40 parts of water. The method comprises the following steps of: sorting, cleaning, squeezing, airing, stirring, fermenting, filtering and packaging. The grape medicinal liquor has the advantages that 1, the nutritive value of each raw material can be integrated into the medicinal liquor fully and effectively by long-time fermentation; 2, the medlar, the mulberry and the liquoric root belong to Chinese herbal medicines, so the grape medicinal liquor has the effects of nourishing liver, improving visual acuity, enriching blood, soothing nerves, enhancing immunity and the like by adding the ingredients into the grape medicinal liquor; 3, a preparation process is simple and easy, high in operability and short in production period, and the work efficiency is improved; and 4, compared with the conventional grape wine, the grape medicinal liquor is strong in fragrance, good in mouthfeel and low in alcohol concentration, is particularly suitable to be drunk by girls and can be used for nourishing skin.