Peptide beverage capable of dispelling effects of alcohol, protecting liver and relieving fatigue as well as preparation method and application of peptide beverage

Abstract

The invention provides a peptide beverage capable of dispelling effects of alcohol, protecting liver and relieving fatigue and a preparation method and application thereof.The peptide beverage comprises hovenia dulcis thunb oligopeptide, walnut peptide, antioxidant peptide, energy release auxiliary peptide and the like, and the antioxidant peptide is prepared by mixing cordyceps militaris, sea-buckthorn and sunflower heads. The energy release auxiliary peptide is prepared from carnosine and arginine succinic acid peptide through microbial fermentation. The preparation method comprises the following steps: mixing the components according to a certain ratio, stirring, homogenizing, sterilizing and the like. The peptide beverage has the effects of dispelling the effects of alcohol, protecting the liver and relieving fatigue, and is suitable for preparing drugs or health-care products for dispelling the effects of alcohol and protecting the liver. The preparation method has the characteristics of strict raw material selection and fine preparation process, and ensures the quality and efficacy of the product. Through a scientific ratio and an advanced preparation technology, the peptide beverage provides a new choice for dispelling the effects of alcohol, protecting the liver and relieving fatigue, and has a wide market application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of biological peptide food processing, and in particular to a peptide drink for sobering up, protecting the liver and relieving fatigue, and a preparation method and application thereof. Background Art

[0002] As the pace of modern life accelerates, people often need to drink alcohol in social and work situations, and excessive drinking often damages the liver and causes physical fatigue. In order to address this problem, a variety of alcohol sobering, liver protection and fatigue relief products have appeared on the market, but most of these products have shortcomings such as insignificant effects, single ingredients or large side effects.

[0003] Traditional alcohol sobering and liver protection products are mostly made of Chinese herbal medicines. Although they can alleviate the damage of alcohol to the liver to a certain extent, their mechanism of action is relatively complex and the effect varies from person to person. In addition, some Chinese herbal medicine ingredients may have potential side effects and pose safety risks to specific groups of people (such as pregnant women and breastfeeding women).

[0004] On the other hand, existing fatigue relief products mostly rely on stimulant ingredients such as caffeine, which can refresh the mind in the short term, but long-term use may lead to adverse reactions such as dependence and insomnia. At the same time, these products often ignore the overall balance and regulation of the body and cannot fundamentally solve the problem of fatigue. Summary of the invention

[0005] In view of this, the present invention proposes a peptide drink for sobering up, protecting the liver and relieving fatigue, as well as a preparation method and application thereof, to solve the above problems.

[0006] The technical scheme of the present invention is achieved as follows: a peptide drink for sobering up, protecting the liver and relieving fatigue: 8-15 parts of Hovenia dulcis oligopeptide, 5-12 parts of walnut peptide, 15-33 parts of antioxidant peptide, 8-12 parts of energy release auxiliary peptide, 0.3-0.5 parts of electrolyte balancer, 2-5 parts of L-carnitine, 0.5-2 parts of nicotinamide adenine dinucleotide, 3-7 parts of oligofructose, and 100-300 parts of water; the antioxidant peptide is a mixed peptide of Cordyceps militaris, sea buckthorn and sunflower disc in a mass ratio of (14-25):(8-10):(3-5); the energy release auxiliary peptide is carnosine and arginine succinate peptide in a mass ratio of 1-3:0.2 prepared by microbial fermentation.

[0007] Furthermore, a peptide drink for sobering up, protecting the liver and relieving fatigue includes the following raw materials in parts by weight: 12 parts of Hovenia dulcis oligopeptide, 10 parts of walnut peptide, 25 parts of antioxidant peptide, 10 parts of energy release auxiliary peptide, 0.4 parts of electrolyte balancer, 3 parts of L-carnitine, 1.2 parts of nicotinamide adenine dinucleotide, 5 parts of oligofructose and 200 parts of water.

[0008] Furthermore, the method comprises the following raw materials in parts by weight: the Hovenia dulcis oligopeptide is prepared by selecting fresh, pest-free and pollution-free Hovenia dulcis as raw material, drying the raw material, crushing the raw material into fine powder, adding water and stirring the raw material, wherein the solid-liquid ratio (g / mL) is 1:1-3, adding a composite enzyme for enzymolysis at 38-45°C and pH 7-9 for 2-4 hours, wherein the composite enzyme is subtilisin and alkaline protease at a mass ratio of 1:2-5, and the enzyme activity is greater than 3000 IU / g, inactivating the enzyme at 80-90°C for 10-15 minutes, centrifuging the raw material, and taking the supernatant for ultrafiltration to obtain the Hovenia dulcis oligopeptide, wherein the molecular weight distribution is less than 1000 Daltons.

[0009] Furthermore, a peptide drink for sobering up, protecting the liver and relieving fatigue is provided. The walnut peptide is prepared by selecting fresh, non-deteriorated walnuts, removing the shells to obtain walnut kernels, crushing the walnut kernels into powder, passing through a 60-100 mesh sieve, soaking the walnut meal powder in a sodium sulfite aqueous solution with a mass percentage concentration of 0.1-0.3%, adding clean water, adjusting the pH value to 7-9 with a dilute alkaline solution, and then performing ultrasonic stirring extraction at a temperature of 50-60°C. This process is performed twice to obtain supernatants a and b, respectively, combining the supernatants for concentration, adding alkaline protease to the concentrate for the first enzymolysis, and then adding neutral protease for the second enzymolysis, subjecting the enzymolysis solution to precipitation treatment, and then centrifuging to obtain a supernatant, which is concentrated to obtain a walnut peptide with a molecular weight of less than 1000 Daltons.

[0010] Furthermore, the amount of enzyme added in the first enzymolysis is 1.5-2% of the volume of the concentrate, the enzymolysis temperature is 30-60°C, the pH is 8-9, and the enzymolysis time is 3-6h. The amount of enzyme added in the second enzymolysis is 0.5-1% of the volume of the concentrate, the enzymolysis temperature is 28-38°C, the pH is 6-7, and the enzymolysis time is 2.5-3.5h.

[0011] Furthermore, the antioxidant peptide is prepared by screening, washing, drying at 60-80°C, and crushing the raw materials of Cordyceps militaris, sea buckthorn, and sunflower disc in the above proportion into 50-80 mesh particles to obtain a mixed powder, adding pure water, wherein the mass volume ratio of the mixed powder to the pure water is 3-5:10 g / mL, obtaining a mixed solution, adjusting the pH value to 7-9, extracting at 90-100°C for 50-70 min, and centrifuging. The supernatant is adjusted to a pH value of 6-7, and bromelain, rhamnosidase, and aminopeptidase are added at a mass ratio of 1: (0.5-0.8): (0.1-0.3) at a temperature of 40-60°C for enzymolysis for 2-4 hours to obtain an enzymolysis solution, separating by ultrafiltration, collecting a polypeptide solution with a molecular weight below 1 kDa, and introducing water vapor to condition at 90-120°C for 3-5 minutes to obtain the antioxidant peptide.

[0012] Furthermore, the energy release auxiliary peptide is prepared by fermentation of carnosine and arginine succinate peptide by microorganisms, wherein the microorganisms are Bacillus and lactic acid bacteria in a mass ratio of (3.5-8.2): (1.2-3.0), and the number of viable bacteria is higher than 2.0×10 8 CFU / g, fermentation temperature is 25-37℃, and fermentation time is 24-72h.

[0013] Furthermore, the electrolyte balance agent is selected from any one or a combination of sodium chloride, potassium chloride, calcium lactate, and magnesium sulfate.

[0014] Furthermore, a preparation method of a peptide drink for sobering up, protecting the liver and relieving fatigue comprises the following steps: Step 1: Add the Hovenia dulcis oligopeptide and walnut peptide into water, stir at 120-150 rpm for 20-30 minutes at a temperature of 40-50°C to fully dissolve them; Step 2: Add antioxidant peptides, energy release auxiliary peptides, L-carnitine, nicotinamide adenine dinucleotide and oligofructose to the above solution in sequence, stir at 80-100 rpm for 15-25 minutes at a temperature of 35-45°C to ensure that all ingredients are evenly mixed; Step 3: Slowly add the electrolyte balance agent to the mixed solution, and stir at a speed of 50-80 rpm for 10-15 minutes to allow the electrolyte balance agent to fully dissolve and evenly disperse in the solution; Step 4: homogenize the mixed solution through a homogenizer at a homogenization pressure of 20-30 MPa for 2-3 times to make the solution more uniform and stable. Sterilize the homogenized solution using a high-temperature instantaneous sterilization method at a sterilization temperature of 120-130°C for 5-10s. Fill and store to obtain a peptide drink that can sober up, protect the liver, and relieve fatigue.

[0015] Furthermore, the peptide drink for sobering up, protecting the liver and relieving fatigue is used in the preparation of sobering up, protecting the liver medicine or health care products.

[0016] Compared with the prior art, the present invention has the following beneficial effects: (1) Significant effect in sobering up and protecting the liver Hovenia dulcis oligopeptides and walnut peptides are rich in various amino acids, which can provide nutritional support for the liver and promote the repair and regeneration of liver cells. The mixed peptides of Cordyceps militaris, Seabuckthorn and Sunflower disk in the antioxidant peptides have a strong antioxidant effect, which can effectively remove free radicals produced by alcohol metabolism and reduce oxidative damage to the liver. Energy release auxiliary peptides help improve the liver's energy metabolism level, enhance the liver's ability to metabolize alcohol, reduce the alcohol concentration in the blood, and reduce the burden on the liver.

[0017] (2) Significant effect in relieving fatigue L-carnitine can promote the entry of fatty acids into mitochondria for oxidation, provide more energy for the body and relieve fatigue. Nicotinamide adenine dinucleotide participates in the cellular energy metabolism process, can improve the body's energy level and enhance endurance. Oligofructose can regulate intestinal flora, promote the absorption of nutrients, and provide continuous energy support for the body.

[0018] (3) Comprehensive nutritional supplement: The beverage also contains a variety of nutrients such as electrolyte balancers and oligofructose. Electrolyte balancers help regulate the electrolyte balance in the body and maintain normal physiological functions; oligofructose, as a prebiotic, can promote intestinal health, enhance immunity, and provide comprehensive nutritional support for the body.

[0019] (4) Advanced preparation technology: The preparation method of the present invention is simple and efficient. Through precise temperature control, stirring speed and homogenization treatment, it ensures that all ingredients are fully dissolved and evenly mixed. At the same time, the high-temperature instantaneous sterilization method is adopted, which not only ensures the hygiene and safety of the product, but also retains the active ingredients in the raw materials to the maximum extent.

[0020] (5) Wide application: The peptide drink of the present invention is not only suitable for sobering up and protecting the liver after daily drinking, but can also be used as a long-term health product to help improve physical fatigue and enhance overall health. Its unique formula and significant efficacy give it broad application prospects in the field of sobering up and protecting the liver or health products. DETAILED DESCRIPTION

[0021] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0022] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0023] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0024] Example 1 A peptide drink for sobering up, protecting the liver and relieving fatigue: 8 parts of Hovenia dulcis oligopeptide, 5 parts of walnut peptide, 15 parts of antioxidant peptide, 8 parts of energy release auxiliary peptide, 0.3 parts of electrolyte balance agent, 2 parts of L-carnitine, 0.5 parts of nicotinamide adenine dinucleotide, 3 parts of oligofructose, and 100 parts of water; The Hovenia dulcis oligopeptide is prepared by selecting fresh, pest-free and pollution-free Hovenia dulcis as raw material, drying it, crushing it into fine powder, adding water and stirring it, with a solid-liquid ratio of g / mL of 1:1, adding a composite enzyme for enzymolysis at 38°C and pH 7 for 2h, wherein the composite enzyme is subtilisin and alkaline protease at a mass ratio of 1:2, and the enzyme activity is greater than 3000 IU / g, inactivating the enzyme at 80-90°C for 10min, centrifuging it, taking the supernatant for ultrafiltration, and obtaining the Hovenia dulcis oligopeptide, whose molecular weight distribution is less than 1000 Daltons; The walnut peptide is prepared by selecting fresh, non-deteriorated walnuts, removing the shells to obtain walnut kernels, crushing the walnut kernels into powder, passing through a 60-mesh sieve, soaking the walnut meal powder in a sodium sulfite aqueous solution with a mass percentage concentration of 0.1%, adding clean water, adjusting the pH value to 7 with a dilute alkaline solution, and then performing ultrasonic stirring extraction at a temperature of 50°C. This process is performed twice to obtain supernatants a and b respectively, combining the supernatants for concentration, adding alkaline protease to the concentrate for the first enzymolysis, and then adding neutral protease for the second enzymolysis, precipitating the enzymolysis solution, and then centrifuging to obtain the supernatant, and concentrating to obtain walnut peptides with a molecular weight of less than 1000 Daltons; the enzyme addition amount of the first enzymolysis is 1.5% of the volume of the concentrate, the enzymolysis temperature is 30°C, the pH is 8, and the enzymolysis time is 3h, and the enzyme addition amount of the second enzymolysis is 0.5% of the volume of the concentrate, the enzymolysis temperature is 28°C, the pH is 6, and the enzymolysis time is 2.5h; The antioxidant peptide is prepared by screening, washing, drying at 60°C, and crushing the raw materials of Cordyceps militaris, sea buckthorn, and sunflower disc in the above proportion into 50 mesh particles to obtain a mixed powder, adding pure water, wherein the mass volume ratio of the mixed powder to the pure water is 3:10 g / mL, obtaining a mixed solution, adjusting the pH value to 7, extracting at 90°C for 50 minutes, and centrifuging the solution, adjusting the pH value of the supernatant to 6, adding bromelain, rhamnosidase, and aminopeptidase at a mass ratio of 1:0.5:0.1, stirring and enzymolysis at 40°C for 2 hours to obtain an enzymolysis solution, ultrafiltration separation, collecting a polypeptide solution with a molecular weight below 1 kDa, and passing water vapor to condition at 90°C for 3 minutes to obtain the antioxidant peptide; The electrolyte balance agent is selected from sodium chloride, potassium chloride, calcium lactate and magnesium sulfate in a mass ratio of 1:1:1:1.

[0025] Example 2 A peptide drink for sobering up, protecting the liver and relieving fatigue: 15 parts of Hovenia dulcis oligopeptide, 12 parts of walnut peptide, 33 parts of antioxidant peptide, 12 parts of energy release auxiliary peptide, 0.5 parts of electrolyte balance agent, 5 parts of L-carnitine, 2 parts of nicotinamide adenine dinucleotide, 7 parts of oligofructose, and 300 parts of water; The Hovenia dulcis oligopeptide is prepared by selecting fresh, pest-free and pollution-free Hovenia dulcis as raw material, drying it, crushing it into fine powder, adding water and stirring it, with a solid-liquid ratio of g / mL of 1:3, adding a composite enzyme for enzymolysis at 45°C and pH 9 for 4 hours, wherein the composite enzyme is subtilisin and alkaline protease at a mass ratio of 1:5, and the enzyme activity is greater than 3000 IU / g, inactivating the enzyme at 90°C for 15 minutes, centrifuging it, taking the supernatant for ultrafiltration, and obtaining the Hovenia dulcis oligopeptide, whose molecular weight distribution is less than 1000 Daltons; The walnut peptide is prepared by selecting fresh, non-deteriorated walnuts, removing the shells to obtain walnut kernels, crushing the walnut kernels into powder, passing through a 100-mesh sieve, soaking the walnut meal powder in a sodium sulfite aqueous solution with a mass percentage concentration of 0.3%, adding clean water, adjusting the pH value to 9 with a dilute alkaline solution, and then performing ultrasonic stirring extraction at a temperature of 60°C. This process is performed twice to obtain supernatants a and b respectively, combining the supernatants for concentration, adding alkaline protease to the concentrate for the first enzymolysis, and then adding neutral protease for the second enzymolysis, precipitating the enzymolysis solution, and then centrifuging to obtain the supernatant, and concentrating to obtain walnut peptides with a molecular weight of less than 1000 Daltons; the enzyme addition amount of the first enzymolysis is 2% of the volume of the concentrate, the enzymolysis temperature is 60°C, the pH is 9, and the enzymolysis time is 6h, and the enzyme addition amount of the second enzymolysis is 1% of the volume of the concentrate, the enzymolysis temperature is 38°C, the pH is 7, and the enzymolysis time is 3.5h; The antioxidant peptide is prepared by screening, washing, drying at 80°C, and crushing the raw materials of Cordyceps militaris, sea buckthorn, and sunflower disc in the above proportion into 80-mesh particles to obtain a mixed powder, adding pure water, wherein the mass volume ratio of the mixed powder to the pure water is 5:10 g / mL, obtaining a mixed solution, adjusting the pH value to 9, extracting at 100°C for 70 minutes, and centrifuging. The supernatant is adjusted to a pH value of 7, adding bromelain, rhamnosidase, and aminopeptidase at a mass ratio of 1:0.8:0.3, stirring and enzymolysis at a temperature of 60°C for 4 hours to obtain an enzymolysis solution, ultrafiltration separation, collecting a polypeptide solution with a molecular weight below 1 kDa, and passing water vapor to condition at 120°C for 5 minutes to obtain the antioxidant peptide; The electrolyte balance agent is selected from sodium chloride, potassium chloride, calcium lactate and magnesium sulfate in a mass ratio of 1:1:1:1.

[0026] Example 3 A peptide drink for sobering up, protecting the liver and relieving fatigue: 12 parts of Hovenia dulcis oligopeptide, 10 parts of walnut peptide, 25 parts of antioxidant peptide, 10 parts of energy release auxiliary peptide, 0.4 parts of electrolyte balance agent, 3 parts of L-carnitine, 1.2 parts of nicotinamide adenine dinucleotide, 5 parts of oligofructose, and 200 parts of water; The Hovenia dulcis oligopeptide is prepared by selecting fresh, pest-free and pollution-free Hovenia dulcis as raw material, drying it, crushing it into fine powder, adding water and stirring it, with a solid-liquid ratio of g / mL of 1:2, adding a composite enzyme for enzymolysis at 42°C and pH 8 for 3 hours, wherein the composite enzyme is subtilisin and alkaline protease at a mass ratio of 1:4, and the enzyme activity is greater than 3000 IU / g, inactivating the enzyme at 80-90°C for 12 minutes, centrifuging it, taking the supernatant for ultrafiltration, and obtaining the Hovenia dulcis oligopeptide, whose molecular weight distribution is less than 1000 Daltons; The walnut peptide is prepared by selecting fresh, non-deteriorated walnuts, removing the shells to obtain walnut kernels, crushing the walnut kernels into powder, passing through an 80-mesh sieve, soaking the walnut meal powder in a sodium sulfite aqueous solution with a mass percentage concentration of 0.2%, adding clean water, adjusting the pH value to 8 with a dilute alkaline solution, and then performing ultrasonic stirring extraction at a temperature of 55°C. This process is performed twice to obtain supernatants a and b respectively, combining the supernatants for concentration, adding alkaline protease to the concentrate for the first enzymolysis, and then adding neutral protease for the second enzymolysis, precipitating the enzymolysis solution, and then centrifuging to obtain the supernatant, and concentrating to obtain walnut peptides with a molecular weight of less than 1000 Daltons; the enzyme addition amount of the first enzymolysis is 1.5-2% of the volume of the concentrate, the enzymolysis temperature is 50°C, the pH is 8, and the enzymolysis time is 5h, and the enzyme addition amount of the second enzymolysis is 0.8% of the volume of the concentrate, the enzymolysis temperature is 30°C, the pH is 6, and the enzymolysis time is 3h; The antioxidant peptide is prepared by screening, washing, drying at 70°C, and crushing the raw materials of Cordyceps militaris, sea buckthorn, and sunflower disc in the above proportion into 70 mesh particles to obtain a mixed powder, adding pure water, wherein the mass volume ratio of the mixed powder to the pure water is 4:10 g / mL, obtaining a mixed solution, adjusting the pH value to 8, extracting at 95°C for 60 minutes, and centrifuging. The supernatant is adjusted to a pH value of 6, and bromelain, rhamnosidase, and aminopeptidase are added at a mass ratio of 1:0.7:0.2, and the supernatant is stirred for enzymolysis at a temperature of 50°C for 3 hours to obtain an enzymolysis solution, ultrafiltration separation, collecting a polypeptide solution with a molecular weight below 1 kDa, and passing water vapor to condition at 100°C for 4 minutes to obtain the antioxidant peptide; The electrolyte balancing agent is selected from sodium chloride, potassium chloride, calcium lactate, and magnesium sulfate in a mass ratio of 1:1:1:1; The above examples 1-3 adopt the following preparation method: Step 1: Add the Hovenia dulcis oligopeptide and walnut peptide into water, stir at 120-150 rpm for 20-30 minutes at a temperature of 40-50°C to fully dissolve them; Step 2: Add antioxidant peptides, energy release auxiliary peptides, L-carnitine, nicotinamide adenine dinucleotide and oligofructose to the above solution in sequence, stir at 80-100 rpm for 15-25 minutes at a temperature of 35-45°C to ensure that all ingredients are evenly mixed; Step 3: Slowly add the electrolyte balance agent to the mixed solution, and stir at a speed of 50-80 rpm for 10-15 minutes to allow the electrolyte balance agent to fully dissolve and evenly disperse in the solution; Step 4: homogenize the mixed solution through a homogenizer at a homogenization pressure of 20-30 MPa for 2-3 times, sterilize the homogenized solution by high-temperature instantaneous sterilization at a temperature of 120-130°C for 5-10 seconds, fill and store, and obtain a peptide drink that can sober up, protect the liver, and relieve fatigue.

[0027] Example 4 A peptide drink for sobering up, protecting the liver and relieving fatigue: 12 parts of Hovenia dulcis oligopeptide, 10 parts of walnut peptide, 25 parts of antioxidant peptide, 10 parts of energy release auxiliary peptide, 0.4 parts of electrolyte balance agent, 3 parts of L-carnitine, 1.2 parts of nicotinamide adenine dinucleotide, 5 parts of oligofructose, and 200 parts of water; The Hovenia dulcis oligopeptide is prepared by selecting fresh, pest-free and pollution-free Hovenia dulcis as raw material, drying it, crushing it into fine powder, adding water and stirring it, with a solid-liquid ratio of g / mL of 1:2, adding a composite enzyme for enzymolysis at 42°C and pH 8 for 3 hours, wherein the composite enzyme is subtilisin and alkaline protease at a mass ratio of 1:4, and the enzyme activity is greater than 3000 IU / g, inactivating the enzyme at 85°C for 12 minutes, centrifuging it, taking the supernatant for ultrafiltration, and obtaining the Hovenia dulcis oligopeptide, whose molecular weight distribution is less than 1000 Daltons; The walnut peptide is prepared by selecting fresh, non-deteriorated walnuts, removing the shells to obtain walnut kernels, crushing the walnut kernels into powder, passing through an 80-mesh sieve, soaking the walnut meal powder in a sodium sulfite aqueous solution with a mass percentage concentration of 0.2%, adding clean water, adjusting the pH value to 8 with a dilute alkaline solution, and then performing ultrasonic stirring extraction at a temperature of 55°C. This process is performed twice to obtain supernatants a and b respectively, combining the supernatants for concentration, adding alkaline protease to the concentrate for the first enzymolysis, and then adding neutral protease for the second enzymolysis, precipitating the enzymolysis solution, and then centrifuging to obtain the supernatant, and concentrating to obtain walnut peptides with a molecular weight of less than 1000 Daltons; the enzyme addition amount of the first enzymolysis is 1.5-2% of the volume of the concentrate, the enzymolysis temperature is 50°C, the pH is 8, and the enzymolysis time is 5h, and the enzyme addition amount of the second enzymolysis is 0.8% of the volume of the concentrate, the enzymolysis temperature is 30°C, the pH is 6, and the enzymolysis time is 3h; The antioxidant peptide is prepared by screening, washing, drying at 70°C, and crushing the raw materials of Cordyceps militaris, sea buckthorn, and sunflower disc in the above proportion into 70 mesh particles to obtain a mixed powder, adding pure water, wherein the mass volume ratio of the mixed powder to the pure water is 4:10 g / mL, obtaining a mixed solution, adjusting the pH value to 8, extracting at 95°C for 60 minutes, and centrifuging. The supernatant is adjusted to a pH value of 6, and bromelain, rhamnosidase, and aminopeptidase are added at a mass ratio of 1:0.7:0.2, and the supernatant is stirred for enzymolysis at a temperature of 50°C for 3 hours to obtain an enzymolysis solution, ultrafiltration separation, collecting a polypeptide solution with a molecular weight below 1 kDa, and passing water vapor to condition at 100°C for 4 minutes to obtain the antioxidant peptide; The electrolyte balancing agent is selected from sodium chloride, potassium chloride, calcium lactate, and magnesium sulfate in a mass ratio of 1:1:1:1; The above-mentioned Example 4 adopts the following preparation method: Step 1: Add the Hovenia dulcis oligopeptide and walnut peptide into water, stir at 120 rpm for 20 min at 40°C to fully dissolve them; Step 2: Add antioxidant peptides, energy release auxiliary peptides, L-carnitine, nicotinamide adenine dinucleotide and oligofructose to the above solution in sequence, and stir at 80 rpm for 15 minutes at a temperature of 35°C to ensure that all ingredients are evenly mixed; Step 3: Slowly add the electrolyte balance agent to the mixed solution and stir at 50 rpm for 10 minutes to fully dissolve the electrolyte balance agent and evenly disperse it in the solution; Step 4: homogenize the mixed solution through a homogenizer at a homogenization pressure of 20 MPa for 2 times, sterilize the homogenized solution by high-temperature instantaneous sterilization at a temperature of 120°C for 5 seconds, fill and store, and obtain a peptide drink that can sober up, protect the liver, and relieve fatigue.

[0028] Example 5 A peptide drink for sobering up, protecting the liver and relieving fatigue: 12 parts of Hovenia dulcis oligopeptide, 10 parts of walnut peptide, 25 parts of antioxidant peptide, 10 parts of energy release auxiliary peptide, 0.4 parts of electrolyte balance agent, 3 parts of L-carnitine, 1.2 parts of nicotinamide adenine dinucleotide, 5 parts of oligofructose, and 200 parts of water; The Hovenia dulcis oligopeptide is prepared by selecting fresh, pest-free and pollution-free Hovenia dulcis as raw material, drying it, crushing it into fine powder, adding water and stirring it, with a solid-liquid ratio of g / mL of 1:2, adding a composite enzyme for enzymolysis at 42°C and pH 8 for 3 hours, wherein the composite enzyme is subtilisin and alkaline protease at a mass ratio of 1:4, and the enzyme activity is greater than 3000 IU / g, inactivating the enzyme at 85°C for 12 minutes, centrifuging it, taking the supernatant for ultrafiltration, and obtaining the Hovenia dulcis oligopeptide, whose molecular weight distribution is less than 1000 Daltons; The walnut peptide is prepared by selecting fresh, non-deteriorated walnuts, removing the shells to obtain walnut kernels, crushing the walnut kernels into powder, passing through an 80-mesh sieve, soaking the walnut meal powder in a sodium sulfite aqueous solution with a mass percentage concentration of 0.2%, adding clean water, adjusting the pH value to 8 with a dilute alkaline solution, and then performing ultrasonic stirring extraction at a temperature of 55°C. This process is performed twice to obtain supernatants a and b respectively, combining the supernatants for concentration, adding alkaline protease to the concentrate for the first enzymolysis, and then adding neutral protease for the second enzymolysis, precipitating the enzymolysis solution, and then centrifuging to obtain the supernatant, and concentrating to obtain walnut peptides with a molecular weight of less than 1000 Daltons; the enzyme addition amount of the first enzymolysis is 1.5-2% of the volume of the concentrate, the enzymolysis temperature is 50°C, the pH is 8, and the enzymolysis time is 5h, and the enzyme addition amount of the second enzymolysis is 0.8% of the volume of the concentrate, the enzymolysis temperature is 30°C, the pH is 6, and the enzymolysis time is 3h; The antioxidant peptide is prepared by screening, washing, drying at 70°C, and crushing the raw materials of Cordyceps militaris, sea buckthorn, and sunflower disc in the above proportion into 70 mesh particles to obtain a mixed powder, adding pure water, wherein the mass volume ratio of the mixed powder to the pure water is 4:10 g / mL, obtaining a mixed solution, adjusting the pH value to 8, extracting at 95°C for 60 minutes, and centrifuging. The supernatant is adjusted to a pH value of 6, and bromelain, rhamnosidase, and aminopeptidase are added at a mass ratio of 1:0.7:0.2, and the supernatant is stirred for enzymolysis at a temperature of 50°C for 3 hours to obtain an enzymolysis solution, ultrafiltration separation, collecting a polypeptide solution with a molecular weight below 1 kDa, and passing water vapor to condition at 100°C for 4 minutes to obtain the antioxidant peptide; The electrolyte balancing agent is selected from sodium chloride, potassium chloride, calcium lactate, and magnesium sulfate in a mass ratio of 1:1:1:1; The above-mentioned Example 5 adopts the following preparation method: Step 1: Add the Hovenia dulcis oligopeptide and walnut peptide into water, stir at 150 rpm for 30 min at 50°C to fully dissolve them; Step 2: Add antioxidant peptides, energy release auxiliary peptides, L-carnitine, nicotinamide adenine dinucleotide and oligofructose to the above solution in sequence, and stir at 100 rpm for 25 minutes at a temperature of 45°C to ensure that all ingredients are evenly mixed; Step 3: Slowly add the electrolyte balance agent to the mixed solution and stir at 80 rpm for 15 minutes to fully dissolve the electrolyte balance agent and evenly disperse it in the solution; Step 4: The mixed solution is homogenized by a homogenizer at a homogenization pressure of 30 MPa for 3 times, and the homogenized solution is sterilized by high-temperature instantaneous sterilization at a temperature of 130°C for 10 seconds. The solution is then filled and stored to obtain a peptide drink that can sober up, protect the liver, and relieve fatigue.

[0029] Comparative Example 1 The difference between this comparative example and Example 3 is that a peptide drink for sobering up, protecting the liver and relieving fatigue comprises the following raw materials in parts by weight: 5 parts of Hovenia dulcis oligopeptide, 2 parts of walnut peptide, 40 parts of antioxidant peptide, 5 parts of energy release auxiliary peptide, 0.6 parts of electrolyte balance agent, 8 parts of L-carnitine, 0.2 parts of nicotinamide adenine dinucleotide, 2 parts of oligofructose, and 400 parts of water.

[0030] Comparative Example 2 The difference between this comparative example and Example 3 is that the raw materials of the peptide drink for sobering up, protecting the liver and relieving fatigue do not contain antioxidant peptides.

[0031] Comparative Example 3 The difference between this comparative example and Example 3 is that the raw materials of the peptide drink for sobering up, protecting the liver and relieving fatigue do not contain energy-releasing auxiliary peptides.

[0032] The difference between this comparative example and Example 3 is that the raw materials of the peptide drink for sobering up, protecting the liver and relieving fatigue do not contain walnut peptide.

[0033] Efficacy verification

[0034] 1. Experimental animals: Select SPF healthy male mice weighing between 20-25g, and start the experiment after acclimatizing to the experimental environment for 1 week. The mice were randomly divided into experimental group, control group and blank group, with 20 mice in each group.

[0035] 2. Experimental materials: Samples to be tested: peptide drinks prepared in Examples 1-5 and Comparative Examples 1-4; Alcohol solution: Choose 50% (v / v) ethanol solution; 3. Experimental steps: (1) Drug administration phase Experimental group: 5 mL / kg of peptide drink was given by gavage every day for 2 weeks.

[0036] Control group: An equal volume of normal saline was administered, and the gavage method and time were the same as those of the experimental group.

[0037] Blank group: no treatment.

[0038] (2) Alcohol gavage One hour after the last administration, mice in the experimental and control groups were gavaged with alcohol solution at 15 ml / kg body weight, and the blank group was given an equal volume of distilled water.

[0039] (3) Sample collection Two hours after alcohol gavage, blood was collected from the eye sockets of the mice, collected in centrifuge tubes, and centrifuged at 3000-5000 rpm for 10-15 minutes at 4°C to separate serum for the determination of blood alcohol concentration.

[0040] At the same time, the mouse liver was quickly removed, rinsed with saline, quickly frozen in liquid nitrogen, and then transferred to a -80°C refrigerator for storage to determine the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in the liver.

[0041] (4) Index detection Blood alcohol concentration determination: Gas chromatography or enzymatic method is used to determine the alcohol concentration in serum; ADH and ALDH activity determination: According to the instructions of the kit, the activities of ADH and ALDH in liver tissue were determined.

[0042] 4. Data results: Blood alcohol concentration (mg / dL) ADH activity (U / mgprotein) ALDH activity (U / mg protein) Example 1 16±5 20±2 12±1.5 Example 2 18±4 21±2.5 13±1.8 Example 3 15±3 22±3 10±2 Example 4 17±3 20±3.5 11±2.2 Example 5 18±2 21±4 12±2.5 Comparative Example 1 30±6 12±1.5 7±1.8 Comparative Example 2 40±5 11±1.8 8±1.2 Comparative Example 3 35±5 10±2 9±1.5 Comparative Example 4 33±4 11±2 9±1.8 Control group 70±10 10±2 6±1.5 Blank Group 4±0.5 5±1 3±0.5 From the above results, it can be seen that through the analysis of blood alcohol concentration, ADH activity and ALDH activity, it can be seen that the peptide drink in the embodiment group showed a good hangover effect, which can effectively reduce blood alcohol concentration, increase the activity of key enzymes in the liver, and accelerate alcohol metabolism.

[0043] Test Example 2-Verification of liver protection efficacy 1. Experimental Animals Healthy SPF adult experimental mice weighing between 20-25 g were selected and randomly divided into an experimental group, a control group and a blank group, with 20 mice in each group.

[0044] 2. Experimental Materials Samples to be tested: peptide drinks prepared in Examples 1-5 and Comparative Examples 1-4 above; Liver damage inducer: carbon tetrachloride (CCl4).

[0045] Detection reagents and instruments: Alanine aminotransferase (ALT), aspartate aminotransferase (AST) kits, fully automatic biochemical analyzer, centrifuge.

[0046] 3. Experimental steps (1) Pre-adaptation stage Experimental group: 5 mL / kg of peptide drink was given by gavage every day for 2 weeks.

[0047] The control group and blank group were given an equal volume of normal saline, and the gavage method and time were the same as those of the experimental group.

[0048] (2) Induction of liver damage A certain dose of liver injury inducer (such as CCl4) was intraperitoneally injected into the mice in the experimental group and the control group, and an equal volume of normal saline was injected into the blank group.

[0049] (3) Sample collection 48 hours after the injection of the liver injury inducer, blood was collected from the eye sockets of the mice, the blood was collected in a centrifuge tube, and centrifuged at 5000 rpm for 15 minutes at 4°C to separate the serum for the determination of the ALT and AST levels in the serum.

[0050] At the same time, the mouse liver was quickly removed and rinsed with physiological saline. Part of the liver tissue was fixed in fixative for subsequent histopathological examination; the other part of the liver tissue was quick-frozen in liquid nitrogen and then transferred to a -80°C refrigerator for storage for the determination of antioxidant indicators in the liver.

[0051] (4) Index detection ALT and AST determination: According to the instructions of the kit, the ALT and AST levels in serum were determined using a fully automatic biochemical analyzer. ALT and AST are important indicators of liver cell damage, and their increased levels indicate liver cell damage.

[0052] Histopathological examination: The fixed liver tissue is sliced ​​and stained (such as hematoxylin-eosin staining), and the morphological and structural changes of liver cells are observed under a microscope, such as whether the liver cells are swollen, necrotic, and whether there is inflammatory cell infiltration.

[0053] Antioxidant index determination: According to the instructions of the kit, the activity of antioxidant enzymes such as SOD and GSH-Px in liver tissue and the content of oxidation products such as malondialdehyde (MDA) were determined. Antioxidant index can reflect the antioxidant capacity of the liver and indirectly reflect the health status of the liver.

[0054] IV. Experimental Results ALT (U / L) AST (U / L) SOD (U / mgprotein) GSH-Px (U / mg protein) MDA (nmol / mg protein) Example 1 26±3 37±4 24±2.5 48±3.5 2.8±0.3 Example 2 27±4 38±5 25±2 45±3 3±0.4 Example 3 25±2 35±3 26±4 55±5 2±0.1 Example 4 28±2 38±3 25±3.5 52±4.5 2.2±0.2 Example 5 29±3 39±4 24±3 50±4 2.5±0.2 Comparative Example 1 38±3 48±4 21±3.5 42±4 3±0.2 Comparative Example 2 50±5 60±6 18±2 35±3 4±0.5 Comparative Example 3 40±3 50±4 20±3 40±4 3.2±0.3 Comparative Example 4 45±4 55±5 19±2.5 38±3.5 3.5±0.4 Control group 80±8 100±10 15±2 30±3 6±1 Blank Group 20±3 30±4 30±4 60±5 1.5±0.3 Through the analysis of indicators such as ALT, AST, SOD, GSH-Px and MDA, it can be seen that the peptide drink in the embodiment group showed good liver protection effect, which can reduce liver damage indicators, increase the activity of liver antioxidant enzymes, reduce the content of oxidation products, and reduce the degree of damage to liver cells.

[0055] Test Example 3 - Anti-fatigue test 1. Experimental Animals Healthy SPF-grade adult mice, weighing between 18 and 22 grams and aged 6 to 8 weeks, were selected and randomly divided into an experimental group, a control group, and a blank group, with 10 mice in each group.

[0056] 2. Experimental Materials (1) Samples to be tested: the peptide drinks prepared in Examples 1-5 and Comparative Examples 1-4 above; (2) Fatigue-inducing devices: such as swimming apparatus (50 cm × 50 cm × 40 cm), running wheels, etc.

[0057] (3) Detection reagents and instruments: lactate test kit, urea nitrogen test kit, blood glucose tester, electronic scale, etc.

[0058] 3. Experimental steps (1) Pre-adaptation stage Experimental group: The peptide drink was given at a dose of 5 mL / kg every day for 2 consecutive weeks.

[0059] The control group and blank group were given an equal volume of normal saline, and the gavage method and time were the same as those of the experimental group.

[0060] (2) Fatigue induction Swimming experiment: Place the mice in the experimental group and the control group into a swimming apparatus and let them swim in water at a temperature of 25±0.5℃ and a depth of 30cm. Record the swimming time of the mice. Stop the experiment when the mice swim to exhaustion (such as sinking into the water for more than 10 seconds and unable to float to the surface).

[0061] Running wheel experiment: Place the mouse on a running wheel, set the speed to 15-20 m / min, and record the time the mouse continues to exercise on the running wheel.

[0062] (3) Sample collection After the fatigue induction experiment, blood was immediately collected from the eye sockets of the mice, and the blood was collected in a centrifuge tube and centrifuged at 3000-5000 rpm for 10-15 min at 4°C to separate the serum for the determination of the lactic acid and urea nitrogen content in the serum.

[0063] At the same time, a blood glucose tester was used to measure the blood glucose levels of the mice.

[0064] (4) Index detection Lactic acid and urea nitrogen determination: According to the instructions of the kit, the content of lactic acid and urea nitrogen in serum was determined. Lactic acid and urea nitrogen are important indicators of fatigue degree, and the increase of their content indicates the aggravation of fatigue degree.

[0065] (5) Blood glucose measurement: Use a blood glucose tester to directly measure the blood glucose level of mice. Blood glucose level can reflect the body's energy metabolism state. In a state of fatigue, blood glucose level may decrease.

[0066] 4. Experimental Data Swimming time (minutes) Wheel running time (minutes) Serum lactate content (mmol / L) Serum urea nitrogen content (mmol / L) Blood glucose level (mmol / L) Example 1 50±4 44±4 4.8±0.4 6.8±0.5 6.2±0.7 Example 2 52±3 46±3 4.6±0.3 6.6±0.4 6.4±0.8 Example 3 55±4 48±4 4.4±0.3 6.4±0.3 6.6±0.9 Example 4 52±5 45±3 4.7±0.4 6.5±0.5 6.0±0.6 Example 5 54±1 46±1 4.6±0.5 6.7±0.4 6.0±0.5 Comparative Example 1 42±3 36±4 5.4±0.3 7.4±0.4 6.0±0.7 Comparative Example 2 40±4 34±3 5.6±0.4 7.6±0.5 5.8±0.6 Comparative Example 3 35±4 30±3 6.0±0.6 8.0±0.7 5.4±0.4 Comparative Example 4 38±3 32±4 5.8±0.5 7.8±0.6 5.6±0.5 Control group 25±3 20±2 7.5±0.8 9.5±0.9 4.5±0.3 Blank Group 20±2 15±2 4.0±0.3 6.0±0.4 6.5±0.4 The swimming time and wheel running time of the mice in Examples 1-5 were significantly longer than those in the control group and the comparative group, and the values ​​were relatively stable. This shows that the peptide drink can significantly improve the exercise endurance of mice, prolong their continuous exercise time, and has a good fatigue relief effect. Through the analysis of indicators such as swimming time, wheel running time, serum lactic acid content, serum urea nitrogen content and blood sugar level, it can be seen that the peptide drink showed a good fatigue relief effect in the embodiment group. It can improve the exercise endurance of mice, reduce the accumulation of fatigue metabolites, maintain blood sugar stability, and provide energy for the body. However, due to different treatment methods, the fatigue relief effect of the comparative group is not as significant as that of the embodiment group. The control group and the blank group represent the fatigue state and the normal state, respectively, which further highlights the role of the peptide drink.

[0067] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A peptide drink for sobering up, protecting the liver and relieving fatigue, characterized by: The invention comprises the following raw materials in parts by weight: 8-15 parts of Hovenia dulcis oligopeptide, 5-12 parts of walnut peptide, 15-33 parts of antioxidant peptide, 8-12 parts of energy release auxiliary peptide, 0.3-0.5 parts of electrolyte balance agent, 2-5 parts of L-carnitine, 0.5-2 parts of nicotinamide adenine dinucleotide, 3-7 parts of oligofructose and 100-300 parts of water; the antioxidant peptide is a mixed peptide of Cordyceps militaris, Seabuckthorn and Sunflower Head in a mass ratio of (14-25):(8-10):(3-5); the energy release auxiliary peptide is prepared by fermenting carnosine and arginine succinate peptide in a mass ratio of 1-3:0.2 through microorganisms.

2. A peptide drink for sobering up, protecting the liver and relieving fatigue as claimed in claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 12 parts of Hovenia dulcis oligopeptide, 10 parts of walnut peptide, 25 parts of antioxidant peptide, 10 parts of energy release auxiliary peptide, 0.4 parts of electrolyte balance agent, 3 parts of L-carnitine, 1.2 parts of nicotinamide adenine dinucleotide, 5 parts of oligofructose and 200 parts of water.

3. A peptide drink for sobering up, protecting liver and relieving fatigue as claimed in claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: the Hovenia dulcis oligopeptide is prepared by selecting fresh, pest-free and pollution-free Hovenia dulcis as raw material, drying the raw material, crushing the raw material into fine powder, adding water for stirring, with a solid-liquid ratio of g / mL of 1:1-3, adding a composite enzyme for enzymolysis at 38-45°C and a pH value of 7-9 for 2-4 hours, wherein the composite enzyme is subtilisin and alkaline protease at a mass ratio of 1:2-5, and the enzyme activity is greater than 3000 IU / g, inactivating the enzyme at 80-90°C for 10-15 minutes, centrifuging the raw material, taking the supernatant for ultrafiltration, and obtaining the Hovenia dulcis oligopeptide, wherein the molecular weight distribution of the composite enzyme is less than 1000 Daltons.

4. The peptide drink for sobering up, protecting liver and relieving fatigue according to claim 1, characterized in that: The walnut peptide is prepared by selecting fresh, non-deteriorated walnuts, removing the shells to obtain walnut kernels, crushing the walnut kernels into powder, passing through a 60-100 mesh sieve, soaking the walnut meal powder in a sodium sulfite aqueous solution with a mass percentage concentration of 0.1-0.3%, adding clean water, adjusting the pH value to 7-9 with a dilute alkaline solution, and then performing ultrasonic stirring extraction at a temperature of 50-60°C. This process is performed twice to obtain supernatants a and b respectively, combining the supernatants for concentration, adding alkaline protease to the concentrate for the first enzymolysis, and then adding neutral protease for the second enzymolysis, precipitating the enzymolysis solution, and then centrifuging to obtain a supernatant, and concentrating to obtain a walnut peptide with a molecular weight of less than 1000 Daltons.

5. A peptide drink for sobering up, protecting liver and relieving fatigue as claimed in claim 4, characterized in that: The enzyme addition amount of the first enzymolysis is 1.5-2% of the volume of the concentrated solution, the enzymolysis temperature is 30-60°C, the pH is 8-9, and the enzymolysis time is 3-6h. The enzyme addition amount of the second enzymolysis is 0.5-1% of the volume of the concentrated solution, the enzymolysis temperature is 28-38°C, the pH is 6-7, and the enzymolysis time is 2.5-3.5h.

6. The peptide drink for sobering up, protecting liver and relieving fatigue according to claim 1, characterized in that: The antioxidant peptide is prepared by screening and washing raw materials of Cordyceps militaris, sea buckthorn and sunflower disc, drying at 60-80°C, and crushing them into 50-80 mesh particles to obtain a mixed powder, adding pure water with a mass volume ratio of the mixed powder to the pure water of 3-5:10 g / mL to obtain a mixed solution, adjusting the pH value to 7-9, extracting at 90-100°C for 50-70 minutes, and centrifuging to adjust the pH value of the supernatant to 6-7, adding bromelain, rhamnosidase and aminopeptidase at a mass ratio of 1:(0.5-0.8):(0.1-0.3), stirring and enzymolysis at a temperature of 40-60°C for 2-4 hours to obtain an enzymolysis solution, ultrafiltration separation, collecting a polypeptide solution with a molecular weight below 1 kDa, and introducing water vapor to condition at 90-120°C for 3-5 minutes to obtain the antioxidant peptide.

7. The peptide drink for sobering up, protecting liver and relieving fatigue according to claim 1, characterized in that: The energy release auxiliary peptide is prepared by fermenting carnosine and arginine succinate peptide through microorganisms. The microorganisms are bacillus and lactic acid bacteria in a mass ratio of (3.5-8.2): (1.2-3.0), and the number of viable bacteria is higher than 2.0×10 8 CFU / g, fermentation temperature is 25-37℃, and fermentation time is 24-72h.

8. The peptide drink for sobering up, protecting liver and relieving fatigue according to claim 1, characterized in that: The electrolyte balance agent is selected from any one or a combination of sodium chloride, potassium chloride, calcium lactate and magnesium sulfate.

9. The method for preparing a peptide drink for sobering up, protecting liver and relieving fatigue as claimed in claim 1, characterized in that: The following steps are involved: Step 1: Add the Hovenia dulcis oligopeptide and walnut peptide into water, stir at 120-150 rpm for 20-30 minutes at a temperature of 40-50°C to fully dissolve them; Step 2: Add antioxidant peptides, energy release auxiliary peptides, L-carnitine, nicotinamide adenine dinucleotide and oligofructose to the solution obtained in step 1 in sequence, and stir at a speed of 80-100 rpm for 15-25 minutes at a temperature of 35-45°C to ensure that the ingredients are evenly mixed; Step 3: Slowly add the electrolyte balance agent to the mixed solution, and stir at a speed of 50-80 rpm for 10-15 minutes to allow the electrolyte balance agent to fully dissolve and evenly disperse in the solution; Step 4: homogenize the mixed solution through a homogenizer at a homogenization pressure of 20-30 MPa for 2-3 times, sterilize the homogenized solution by high-temperature instantaneous sterilization at a temperature of 120-130°C for 5-10 seconds, fill and store, and obtain a peptide drink that can sober up, protect the liver, and relieve fatigue.

10. Use of the peptide drink for sobering up, protecting the liver and relieving fatigue according to any one of claims 1 to 8 in the preparation of sobering up, protecting the liver medicine or health care product.