Skin bacteriostatic liquid containing plant extract and preparation method thereof

Abstract

The application relates to the technical field of skin bacteriostatic liquid, and discloses a skin bacteriostatic liquid containing plant extracts and a preparation method thereof, which comprises the following raw materials in parts by mass: composite plant extracts containing nanocrystalline particles 15-20 parts, glycerol 3-4 parts, sodium hyaluronate 0.1-0.2 parts and purified water 75-85 parts. The composite plant extracts containing nanocrystalline particles, glycerol, sodium hyaluronate and purified water are mixed to obtain the skin bacteriostatic liquid, which has the advantages of strong bacteriostasis, long-acting bacteriostasis, mildness and no irritation, and can repair the skin barrier, realize long-acting skin bacteriostasis, reduce the repeated occurrence of skin inflammation, and relieve inflammatory skin aging.

Description

Technical Field

[0001] This invention relates to the field of skin antibacterial solution technology, specifically to a skin antibacterial solution containing plant extracts and its preparation method. Background Technology

[0002] People with weak skin resistance are easily infected by bacteria, leading to various skin diseases. Escherichia coli is a Gram-negative bacterium that can cause pneumonia, peritonitis, meningitis, and other diseases under certain conditions. Children and the elderly, in particular, are more susceptible to severe infections caused by E. coli, and in severe cases, it can be life-threatening. Staphylococcus aureus is the second most pathogenic bacterium for humans after E. coli. When the skin is injured, it can easily cause local purulent infections, leading to infectious diarrhea, pneumonia, pericarditis, and other diseases. It also has strong drug resistance.

[0003] Currently available antibacterial skin solutions rely heavily on chemical disinfectants, which can lead to dry, damaged, and irritated skin, and even the development of drug-resistant strains with long-term use. Antibacterial skin solutions are topical liquid products applied to the skin surface to inhibit the growth of microorganisms such as bacteria and fungi. Their formulas often contain plant extracts, inorganic salts, and other ingredients. In existing technologies, antibacterial skin solutions containing plant extracts mostly rely on plant extracts for antibacterial activity, which is only effective against a single bacterial species and has a narrow antibacterial spectrum. Furthermore, their strong and long-lasting antibacterial effects are relatively low.

[0004] There is an urgent need to develop a skin antibacterial liquid that is highly effective, long-lasting, and contains plant extracts. Summary of the Invention

[0005] This invention provides a skin antibacterial liquid containing plant extracts and its preparation method, which solves the problem that asphalt is easily affected by environmental factors such as heat, oxygen and ultraviolet radiation, causing its internal groups to undergo oxidation reactions, accompanied by molecular condensation and rearrangement, leading to the gradual embrittlement and hardening of asphalt, and thus inducing cracking and loosening.

[0006] The technical solution of the present invention: A skin antibacterial liquid containing plant extracts comprises the following raw materials in parts by weight: 15-20 parts of a complex plant extract containing nanocrystals, 3-4 parts of glycerin, 0.1-0.2 parts of sodium hyaluronate, and 75-85 parts of purified water; The composite plant extract containing nanocrystals is obtained by mixing selenium-enriched chickpea sprout extract, composite plant extract and phloretin as the oil phase, hydroxypropyl methylcellulose as the aqueous phase, and the oil phase and aqueous phase are mixed. The selenium-enriched chickpea sprout extract is specifically obtained by: cultivating chickpeas in a selenium-enriched culture to obtain selenium-enriched chickpea sprouts, and then extracting the product from them to obtain the selenium-enriched chickpea sprout extract.

[0007] A method for preparing a skin antibacterial liquid containing plant extracts includes the following preparation steps: Mix the composite plant extract containing nanocrystals, glycerin, sodium hyaluronate and purified water, stir at 80-100 r / min for 20-40 min, and sterilize at 70-75℃ for 25-30 min to obtain the skin antibacterial solution.

[0008] Furthermore, the compound plant extract is composed of rhubarb extract, licorice extract, and angelica extract mixed in a mass ratio of (8-10):(8-10):1.

[0009] Furthermore, the purified water has a salt concentration of 0.3-0.8 mg / L and a resistivity of 1.0-2.0 × 10⁻⁶ at 25°C. 6 Ω·cm.

[0010] Furthermore, the pH of the skin antibacterial solution is 5.5-6.0.

[0011] Furthermore, the composite plant extract containing nanocrystals is specifically prepared by the following steps: A1. After washing chickpea seeds with sodium hypochlorite solution, wash them with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Add the cleaned chickpea seeds and sodium selenite to deionized water and hydrate them at 23-27℃ for 5-6 hours. Collect the hydrated seeds and place them in an incubator for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts, grind them in a grinder and sieve them to obtain selenium-enriched chickpea sprout powder. Add the selenium-enriched chickpea sprout powder to hexane and stir at 45-55℃ for 12-13 hours. After filtration, place it in deionized water, adjust the pH with sodium hydroxide solution, stir, and then centrifuge. Collect the supernatant a and powder pellets. Mix the powder pellets with deionized water, stir, and centrifuge again to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. Add selenium-enriched chickpea sprout extract, compound plant extract and phloretin to ethanol and stir well to form the oil phase. Add hydroxypropyl methylcellulose to deionized water to form the aqueous phase. Mix the oil phase and aqueous phase and stir at 1000-1500 r / min for 2-5 min. Remove the ethanol by vacuum rotary evaporation to obtain the compound plant extract containing nanocrystals.

[0012] Furthermore, in the A1 reaction process described above, after the chickpea seeds are disinfected with sodium hypochlorite solution, they germinate in the presence of trace amounts of sodium selenite to obtain selenium-enriched chickpea sprouts. The selenium element inhibits the degradation of proteins in the chickpea sprout extract, resulting in sufficient protein content in the selenium-enriched chickpea sprouts.

[0013] Furthermore, in the A2 reaction process described above, after the selenium-enriched chickpea sprout powder is defatted in hexane, the protease in the selenium-enriched chickpea sprout powder is dissolved in deionized water. Using deionized water as the extraction agent, the protein of the selenium-enriched chickpea sprout powder is extracted from the chickpea sprout powder into an aqueous solution. The activity of the protease in the aqueous solution is measured. The addition of sodium hydroxide helps the protease extraction, enhances the stability of the protein hydrolysate, and improves its antioxidant capacity.

[0014] Furthermore, in the above-mentioned A3 reaction process, selenium-enriched chickpea sprout extract, plant extract and phloretin are in an ethanol solution as the organic phase. In the ethanol, the selenium-enriched chickpea sprout extract, plant extract and phloretin are fully dissolved and mixed. Hydrogen bonds and π-π conjugated stacking are formed between the phenolic hydroxyl groups, polysaccharide hydroxyl groups and benzene ring structures of each component, forming a multi-component complex molecular precursor solution, avoiding the sudden precipitation of coarse crystals in some areas. Hydroxypropyl methylcellulose is used as the aqueous phase in deionized water and mixed with the oil phase. The deionized water acts as an antisolvent, which has low solubility for fat-soluble selenium-enriched chickpea sprout extract, plant extracts and phloretin. This causes the fat-soluble selenium-enriched chickpea sprout extract, plant extracts and phloretin to rapidly precipitate out as crystals due to supersaturation. Hydroxypropyl methylcellulose can be adsorbed on the crystal surface, providing a steric hindrance effect, inhibiting crystal aggregation, controlling the particle size to maintain the nanoscale, and forming a composite plant extract containing nanocrystals.

[0015] Further, in step A1, the mass ratio of the cleaned chickpea seeds, sodium selenite, and deionized water is 1:(0.01-0.03):(80-90).

[0016] Further, in step A2, the mass ratio of the selenium-enriched chickpea sprout powder, hexane, and deionized water is 1:(6-10):(20-30).

[0017] Further, in step A3, the mass ratio of the selenium-enriched chickpea sprout extract, the compound plant extract, the phloretin, and the ethanol is 1:(2-2.5):(0.3-0.5):(15-20).

[0018] Further, in step A3, the mass ratio of hydroxypropyl methylcellulose to deionized water is 1:(100-120).

[0019] Furthermore, in step A3, the mass ratio of the oil phase to the water phase is 1:(4-4.5).

[0020] The present invention has the following beneficial effects: (1) In the technical solution of the present invention, the plant extracts include rhubarb extract, licorice extract and angelica extract, which contain anthraquinone compounds, anthrone compounds, tannins and other chemical substances. They have inhibitory effects on bacteria such as Escherichia coli and Staphylococcus aureus, and also have certain anti-inflammatory effects. In addition, licorice extract can promote the inhibitory effect of rhubarb extract on Escherichia coli and Staphylococcus aureus. Furthermore, angelica extract has the effect of promoting transdermal absorption. The combination of the three achieves the effects of broad-spectrum antibacterial, reduced irritation and long-lasting transdermal absorption.

[0021] (2) In the technical solution of the present invention, chickpeas are cultured with selenium to obtain selenium-enriched chickpea sprouts, and the products are extracted to obtain selenium-enriched chickpea sprout extract. On the one hand, chickpea sprout extract can inhibit the degradation of skin collagen and elastin, while promoting collagen regeneration. It can repair the skin barrier and improve skin elasticity, so that the antibacterial liquid can take into account both antibacterial care and anti-aging repair effects, reduce the skin inflammation, redness, itching, and pigmentation of acne marks caused by frequent use of antibacterial liquid, and thus reduce skin oxidative damage. On the other hand, selenium-enriched chickpea sprout extract increases the protein content in the hydrolysate. The protein in the hydrolysate has good antioxidant activity, and can enhance the synthesis of collagen and promote the proliferation of fibroblasts, repair the skin barrier, achieve long-term skin antibacterial effect, reduce the recurrence of skin inflammation, and alleviate inflammatory skin aging.

[0022] (3) In the technical solution of the present invention, selenium-enriched chickpea sprout extract, plant extract and phloretin are mixed as oil phase, and hydroxypropyl methylcellulose is used as aqueous phase. The oil phase and aqueous phase are mixed to obtain a composite plant extract containing nanocrystals. On the one hand, the composite plant extract containing nanocrystals has a small size effect, which is conducive to skin absorption and achieves slow penetration into the superficial epidermis. It upgrades from surface antibacterial to superficial long-term antibacterial, solving the problem that ordinary antibacterial liquids only stay on the skin surface and cannot improve superficial flora imbalance. In addition, hydroxypropyl methylcellulose can be adsorbed on the crystal surface to provide a steric hindrance effect. It inhibits crystal aggregation. In addition, it can encapsulate plant extracts inside nanocrystals, which can improve the stability of the antibacterial solution. On the other hand, phlorizin has antioxidant and free radical scavenging effects. As a lipophilic compound, phlorizin can change the membrane dipole potential of the skin phospholipid bilayer, affect the interfacial structure of the phospholipid bilayer and the water flow dynamics driven by water permeation, and penetrate into the skin through intercellular lipids. Phlorizin, as a medium, can increase the transdermal effect of the complex plant extract containing nanocrystals, promote the penetration and absorption of the complex plant extract containing nanocrystals on the skin, and achieve a long-lasting antibacterial effect.

[0023] (4) In the technical solution of the present invention, a skin antibacterial solution is obtained by mixing a composite plant extract containing nanocrystals, glycerin, sodium hyaluronate and purified water. It has strong antibacterial effect, long-lasting antibacterial effect, mild and non-irritating properties, and can repair the skin barrier, thereby achieving long-lasting skin antibacterial effect, reducing the recurrence of skin inflammation and alleviating inflammatory skin aging. Detailed Implementation

[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0025] The raw materials used in the embodiments of this invention are shown below, and all reagents used are analytical grade.

[0026] The pH of the skin antibacterial solution is 5.5-6.0.

[0027] The purified water has a salt concentration of 0.3-0.8 mg / L and a resistivity of 1.0-2.0 × 10⁻⁶ at 25℃. 6 Ω·cm.

[0028] The preparation steps for rhubarb extract, licorice extract, and angelica dahurica extract are as follows: Rhubarb, licorice, and angelica were pulverized separately to obtain powders. After passing the powders through an 80-mesh sieve and drying at 80°C, the powders were weighed separately and then added to an 85% ethanol aqueous solution. The mixture was ultrasonically treated at 35°C. After ultrasonication, the solution was poured into a centrifuge tube, centrifuged, filtered, and the supernatant was collected. The ethanol was combined and concentrated to obtain powdered rhubarb extract, licorice extract, and angelica extract. The specific steps of ultrasonic treatment were as follows: each ultrasonic treatment lasted 30 minutes, and the ultrasonication was repeated 3 times. The mass ratio of powder to ethanol aqueous solution was 1:20.

[0029] Hydroxypropyl methylcellulose, 2% viscosity: 3 mPa·s, oxygen content: 28-30%; hydroxypropyl content: 7.0-12%.

[0030] Example 1 A skin antibacterial liquid containing plant extracts comprises the following raw materials in parts by weight: 15 parts of a complex plant extract containing nanocrystals, 3 parts of glycerin, 0.1 parts of sodium hyaluronate, and 75 parts of purified water. A method for preparing a skin antibacterial liquid containing plant extracts includes the following preparation steps: A skin antibacterial solution was obtained by mixing a complex plant extract containing nanocrystals, glycerin, sodium hyaluronate, and purified water, stirring at 80 rpm for 20 min, and sterilizing at 70°C for 25 min.

[0031] The composite plant extract containing nanocrystals is prepared by the following steps: A1. Wash chickpea seeds with a 5% sodium hypochlorite solution for 3 minutes, then wash with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Cleaned chickpea seeds, sodium selenite, and deionized water were mixed at a mass ratio of 1:0.01:80 and hydrated at 23°C for 5 hours, stirring continuously every 15 minutes. The hydrated seeds were collected and placed in an incubator at 24°C and 80% relative humidity for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts at -72℃ for 36 hours, grind them in a grinder, pass them through a 60-mesh sieve, and store them at -20℃ to obtain selenium-enriched chickpea sprout powder. Selenium-enriched chickpea sprout powder was added to hexane and stirred at 45°C for 12 hours, with the hexane being replaced every 6 hours. After filtration, the mixture was placed in deionized water, and the pH was adjusted to 8.5 with 36% sodium hydroxide. The mixture was stirred for 2 hours, and then centrifuged at 10000 r / min for 20 minutes. The supernatant a and the powder pellets were collected. The mass ratio of selenium-enriched chickpea sprout powder, hexane, and deionized water was 1:6:20. Mix the powdered spheres and deionized water at a mass ratio of 1:20, stir evenly, and centrifuge again at a speed of 10000 r / min for 20 min to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. The compound plant extract was prepared by mixing rhubarb extract, licorice extract, and angelica extract in a mass ratio of 8:8:1; selenium-enriched chickpea sprout extract, compound plant extract, phloretin, and ethanol were mixed in a mass ratio of 1:2:0.3:15 and stirred evenly to form the oil phase; hydroxypropyl methylcellulose and deionized water were mixed in a mass ratio of 1:100 and stirred evenly to form the aqueous phase; the oil phase and aqueous phase were mixed in a mass ratio of 1:4 and stirred at 1000 r / min for 2 min; ethanol was removed by rotary evaporation under reduced pressure at 45℃, -0.05 MPa, and 120 r / min to obtain the compound plant extract containing nanocrystals.

[0032] Example 2 A skin antibacterial liquid containing plant extracts comprises the following raw materials in parts by weight: 18 parts of a complex plant extract containing nanocrystals, 3.5 parts of glycerin, 0.15 parts of sodium hyaluronate, and 80 parts of purified water; A method for preparing a skin antibacterial liquid containing plant extracts includes the following preparation steps: A skin antibacterial solution was obtained by mixing a complex plant extract containing nanocrystals, glycerin, sodium hyaluronate, and purified water, stirring at 90 rpm for 30 min, and sterilizing at 73°C for 28 min.

[0033] The composite plant extract containing nanocrystals is prepared by the following steps: A1. Wash chickpea seeds with a 5% sodium hypochlorite solution for 3 minutes, then wash with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Cleaned chickpea seeds, sodium selenite, and deionized water were mixed at a mass ratio of 1:0.02:85 and hydrated at 25°C for 5.5 hours, stirring continuously every 15 minutes. The hydrated seeds were collected and placed in an incubator at 24°C and 80% relative humidity for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts at -72℃ for 36 hours, grind them in a grinder, pass them through a 60-mesh sieve, and store them at -20℃ to obtain selenium-enriched chickpea sprout powder. Selenium-enriched chickpea sprout powder was added to hexane and stirred at 50°C for 12.5 hours, with the hexane being replaced every 6 hours. After filtration, the mixture was placed in deionized water, and the pH was adjusted to 8.5 with 36% sodium hydroxide. The mixture was stirred for 2 hours, and then centrifuged at 10000 r / min for 20 minutes. The supernatant a and the powder pellets were collected. The mass ratio of selenium-enriched chickpea sprout powder, hexane, and deionized water was 1:8:25. The powdered spheres and deionized water were mixed at a mass ratio of 1:25 and stirred evenly. The mixture was then centrifuged again at a speed of 10000 r / min for 20 min to obtain supernatant b. Supernatant a and supernatant b were mixed to obtain selenium-enriched chickpea sprout extract. A3. The compound plant extract was prepared by mixing rhubarb extract, licorice extract, and angelica extract in a mass ratio of 9:9:1; selenium-enriched chickpea sprout extract, compound plant extract, phloretin, and ethanol were mixed in a mass ratio of 1:2.3:0.4:18 and stirred evenly to form the oil phase; hydroxypropyl methylcellulose and deionized water were mixed in a mass ratio of 1:110 and stirred evenly to form the aqueous phase; the oil phase and aqueous phase were mixed in a mass ratio of 1:4.3 and stirred at 1300 r / min for 3 min; ethanol was removed by rotary evaporation under reduced pressure at 45℃, -0.05 MPa, and 120 r / min to obtain the compound plant extract containing nanocrystals.

[0034] Example 3 A skin antibacterial liquid containing plant extracts comprises the following raw materials in parts by weight: 20 parts of a complex plant extract containing nanocrystals, 4 parts of glycerin, 0.2 parts of sodium hyaluronate, and 85 parts of purified water; A method for preparing a skin antibacterial liquid containing plant extracts includes the following preparation steps: A skin antibacterial solution was obtained by mixing a complex plant extract containing nanocrystals, glycerin, sodium hyaluronate, and purified water, stirring at 100 rpm for 40 min, and sterilizing at 75°C for 30 min.

[0035] The composite plant extract containing nanocrystals is prepared by the following steps: A1. Wash chickpea seeds with a 5% sodium hypochlorite solution for 3 minutes, then wash with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Cleaned chickpea seeds, sodium selenite, and deionized water were mixed at a mass ratio of 1:0.03:90 and hydrated at 27°C for 6 hours with continuous stirring every 15 minutes. The hydrated seeds were collected and placed in an incubator at 24°C and 80% relative humidity for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts at -72℃ for 36 hours, grind them in a grinder, pass them through a 60-mesh sieve, and store them at -20℃ to obtain selenium-enriched chickpea sprout powder. Selenium-enriched chickpea sprout powder was added to hexane and stirred at 55°C for 13 hours, with the hexane being replaced every 6 hours. After filtration, the mixture was placed in deionized water, and the pH was adjusted to 8.5 with 36% sodium hydroxide. The mixture was stirred for 2 hours, and then centrifuged at 10000 r / min for 20 minutes. The supernatant a and the powder pellets were collected. The mass ratio of selenium-enriched chickpea sprout powder, hexane, and deionized water was 1:10:30. Mix the powdered spheres and deionized water at a mass ratio of 1:30, stir evenly, and centrifuge again at a speed of 10000 r / min for 20 min to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. The compound plant extract was prepared by mixing rhubarb extract, licorice extract, and angelica extract in a mass ratio of 10:10:1; selenium-enriched chickpea sprout extract, compound plant extract, phloretin, and ethanol were mixed in a mass ratio of 1:2.5:0.5:20 and stirred evenly to form the oil phase; hydroxypropyl methylcellulose and deionized water were mixed in a mass ratio of 1:120 and stirred evenly to form the aqueous phase; the oil phase and aqueous phase were mixed in a mass ratio of 1:4.5 and stirred at 1500 r / min for 5 min; ethanol was removed by rotary evaporation under reduced pressure at 45℃, -0.05 MPa, and 120 r / min to obtain a compound plant extract containing nanocrystals.

[0036] Comparative Example 1 The only difference between this comparative example and Example 3 is the preparation of the composite plant extract containing nanocrystals, as detailed below: The composite plant extract containing nanocrystals is prepared by the following steps: The compound plant extract was prepared by mixing rhubarb extract, licorice extract, and angelica extract in a mass ratio of 10:10:1. Chickpea sprout extract, the compound plant extract, phloretin, and ethanol were mixed in a mass ratio of 1:2.5:0.5:20 and stirred evenly to form the oil phase. Hydroxypropyl methylcellulose and deionized water were mixed in a mass ratio of 1:120 and stirred evenly to form the aqueous phase. The oil phase and the aqueous phase were mixed in a mass ratio of 1:4.5 and stirred at 1500 r / min for 5 min. Ethanol was removed by rotary evaporation under reduced pressure at 45℃, -0.05 MPa, and 120 r / min to obtain the compound plant extract containing nanocrystals.

[0037] Comparative Example 2 The only difference between this comparative example and Example 3 is the preparation of the composite plant extract containing nanocrystals, as detailed below: The composite plant extract containing nanocrystals is prepared by the following steps: A1. Wash chickpea seeds with a 5% sodium hypochlorite solution for 3 minutes, then wash with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Cleaned chickpea seeds, sodium selenite, and deionized water were mixed at a mass ratio of 1:0.03:90 and hydrated at 27°C for 6 hours with continuous stirring every 15 minutes. The hydrated seeds were collected and placed in an incubator at 24°C and 80% relative humidity for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts at -72℃ for 36 hours, grind them in a grinder, pass them through a 60-mesh sieve, and store them at -20℃ to obtain selenium-enriched chickpea sprout powder. Selenium-enriched chickpea sprout powder was added to hexane and stirred at 55°C for 13 hours, with the hexane being replaced every 6 hours. After filtration, the mixture was placed in deionized water, and the pH was adjusted to 8.5 with 36% sodium hydroxide. The mixture was stirred for 2 hours, and then centrifuged at 10000 r / min for 20 minutes. The supernatant a and the powder pellets were collected. The mass ratio of selenium-enriched chickpea sprout powder, hexane, and deionized water was 1:10:30. Mix the powdered spheres and deionized water at a mass ratio of 1:30, stir evenly, and centrifuge again at a speed of 10000 r / min for 20 min to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. The compound plant extract was prepared by mixing rhubarb extract, licorice extract, and angelica extract in a mass ratio of 10:10:1; selenium-enriched chickpea sprout extract, the compound plant extract, and ethanol were mixed in a mass ratio of 1.5:2.5:20 and stirred evenly to form the oil phase; hydroxypropyl methylcellulose and deionized water were mixed in a mass ratio of 1:120 and stirred evenly to form the aqueous phase; the oil phase and the aqueous phase were mixed in a mass ratio of 1:4.5 and stirred at 1500 r / min for 5 min; ethanol was removed by rotary evaporation under reduced pressure at 45℃, -0.05 MPa, and 120 r / min to obtain the compound plant extract containing nanocrystals.

[0038] Comparative Example 3 The only difference between this comparative example and Example 3 is the preparation of the composite plant extract containing nanocrystals, as detailed below: The composite plant extract containing nanocrystals is prepared by the following steps: A1. Wash chickpea seeds with a 5% sodium hypochlorite solution for 3 minutes, then wash with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Cleaned chickpea seeds, sodium selenite, and deionized water were mixed at a mass ratio of 1:0.03:90 and hydrated at 27°C for 6 hours with continuous stirring every 15 minutes. The hydrated seeds were collected and placed in an incubator at 24°C and 80% relative humidity for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts at -72℃ for 36 hours, grind them in a grinder, pass them through a 60-mesh sieve, and store them at -20℃ to obtain selenium-enriched chickpea sprout powder. Selenium-enriched chickpea sprout powder was added to hexane and stirred at 55°C for 13 hours, with the hexane being replaced every 6 hours. After filtration, the mixture was placed in deionized water, and the pH was adjusted to 8.5 with 36% sodium hydroxide. The mixture was stirred for 2 hours, and then centrifuged at 10000 r / min for 20 minutes. The supernatant a and the powder pellets were collected. The mass ratio of selenium-enriched chickpea sprout powder, hexane, and deionized water was 1:10:30. Mix the powdered spheres and deionized water at a mass ratio of 1:30, stir evenly, and centrifuge again at a speed of 10000 r / min for 20 min to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. The compound plant extract was prepared by mixing licorice extract and angelica extract at a mass ratio of 20:1; selenium-enriched chickpea sprout extract, compound plant extract, phloretin, and ethanol were mixed at a mass ratio of 1:2.5:0.5:20 and stirred evenly to form the oil phase; hydroxypropyl methylcellulose and deionized water were mixed at a mass ratio of 1:120 and stirred evenly to form the aqueous phase; the oil phase and aqueous phase were mixed at a mass ratio of 1:4.5 and stirred at 1500 r / min for 5 min; ethanol was removed by rotary evaporation under reduced pressure at 45℃, -0.05 MPa, and 120 r / min to obtain a compound plant extract containing nanocrystals.

[0039] Comparative Example 4 The only difference between this comparative example and Example 3 is the preparation of the composite plant extract containing nanocrystals, as detailed below: The composite plant extract containing nanocrystals is prepared by the following steps: A1. Wash chickpea seeds with a 5% sodium hypochlorite solution for 3 minutes, then wash with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Cleaned chickpea seeds, sodium selenite, and deionized water were mixed at a mass ratio of 1:0.03:90 and hydrated at 27°C for 6 hours with continuous stirring every 15 minutes. The hydrated seeds were collected and placed in an incubator at 24°C and 80% relative humidity for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts at -72℃ for 36 hours, grind them in a grinder, pass them through a 60-mesh sieve, and store them at -20℃ to obtain selenium-enriched chickpea sprout powder. Selenium-enriched chickpea sprout powder was added to hexane and stirred at 55°C for 13 hours, with the hexane being replaced every 6 hours. After filtration, the mixture was placed in deionized water, and the pH was adjusted to 8.5 with 36% sodium hydroxide. The mixture was stirred for 2 hours, and then centrifuged at 10000 r / min for 20 minutes. The supernatant a and the powder pellets were collected. The mass ratio of selenium-enriched chickpea sprout powder, hexane, and deionized water was 1:10:30. Mix the powdered spheres and deionized water at a mass ratio of 1:30, stir evenly, and centrifuge again at a speed of 10000 r / min for 20 min to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. The compound plant extract was prepared by mixing rhubarb extract and angelica extract at a mass ratio of 10:11; selenium-enriched chickpea sprout extract, compound plant extract, phloretin, and ethanol were mixed at a mass ratio of 1:2.5:0.5:20 and stirred evenly to form the oil phase; hydroxypropyl methylcellulose and deionized water were mixed at a mass ratio of 1:120 and stirred evenly to form the aqueous phase; the oil phase and aqueous phase were mixed at a mass ratio of 1:4.5 and stirred at 1500 r / min for 5 min; ethanol was removed by rotary evaporation under reduced pressure at 45℃, -0.05 MPa, and 120 r / min to obtain a compound plant extract containing nanocrystals.

[0040] Comparative Example 5 The only difference between this comparative example and Example 3 is the preparation of the skin antibacterial solution, as detailed below: A skin antibacterial liquid containing plant extracts comprises the following raw materials in parts by weight: 20 parts of mixed extract, 4 parts of glycerin, 0.2 parts of sodium hyaluronate, and 85 parts of purified water; A method for preparing a skin antibacterial liquid containing plant extracts includes the following preparation steps: Mix the extract, glycerin, sodium hyaluronate and purified water, stir at 100 r / min for 40 min, and sterilize at 75℃ for 30 min to obtain the skin antibacterial solution.

[0041] The mixed extract is prepared by the following steps: A1. Wash chickpea seeds with a 5% sodium hypochlorite solution for 3 minutes, then wash with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Cleaned chickpea seeds, sodium selenite, and deionized water were mixed at a mass ratio of 1:0.03:90 and hydrated at 27°C for 6 hours with continuous stirring every 15 minutes. The hydrated seeds were collected and placed in an incubator at 24°C and 80% relative humidity for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts at -72℃ for 36 hours, grind them in a grinder, pass them through a 60-mesh sieve, and store them at -20℃ to obtain selenium-enriched chickpea sprout powder. Selenium-enriched chickpea sprout powder was added to hexane and stirred at 55°C for 13 hours, with the hexane being replaced every 6 hours. After filtration, the mixture was placed in deionized water, and the pH was adjusted to 8.5 with 36% sodium hydroxide. The mixture was stirred for 2 hours, and then centrifuged at 10000 r / min for 20 minutes. The supernatant a and the powder pellets were collected. The mass ratio of selenium-enriched chickpea sprout powder, hexane, and deionized water was 1:10:30. Mix the powdered spheres and deionized water at a mass ratio of 1:30, stir evenly, and centrifuge again at a speed of 10000 r / min for 20 min to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. The compound plant extract is composed of rhubarb extract, licorice extract, and angelica extract in a mass ratio of 10:10:1; selenium-enriched chickpea sprout extract, compound plant extract, phloretin, and ethanol are mixed in a mass ratio of 1:2.5:0.5:20, stirred evenly, and the ethanol is removed by rotary evaporation under reduced pressure at 45℃, -0.05MPa, and 120r / min to obtain the mixed extract.

[0042] The performance of the antibacterial skin solutions prepared in Examples 1-3 and Comparative Examples 1-5 was then tested.

[0043] Antibacterial activity test: Antibacterial tests were conducted according to the "Disinfection Technical Specifications" (2002 edition) to detect the antibacterial rate against Staphylococcus aureus (ATCC6538) and Escherichia coli (ATCC25922). The test bacterial concentration was 1×10⁻⁶. 6 -5×10 6 CFU / mL, action time 2 minutes, 3 parallel tests for each sample, and average value; inhibition rate (%) = [(number of viable bacteria in control group - number of viable bacteria in test group) / number of viable bacteria in control group] × 100%.

[0044] Stability testing: The prepared antibacterial skin solution was stored at 25±2℃ and 75±5% relative humidity for 6 months. The appearance of the preparation was observed to check for precipitation, layering, and turbidity. The retention rate of active ingredients was also tested (tannin and salvianolic acid B content were detected by high performance liquid chromatography). Stability judgment criteria: clear without precipitation, layering, or turbidity; retention rate of active ingredients ≥85% is considered qualified.

[0045] Skin irritation test: An acute skin irritation test was conducted on rabbits according to the "Cosmetic Safety Technical Specifications" (2015 edition). Six healthy New Zealand white rabbits were selected, and 0.5 mL of the prepared antibacterial skin solution was applied to the hairless skin on the back of each rabbit (area 2.5 cm × 2.5 cm). The skin was kept in contact for 4 hours, and skin reactions were observed at 24 h, 48 h, and 72 h. The irritation level was determined according to the scoring criteria. Irritation levels: No irritation (0 points), mild irritation (1-2 points), moderate irritation (3-5 points), severe irritation (6-8 points).

[0046] The test results are shown in Tables 1, 2, and 3 below.

[0047] Table 1. Performance testing of the antibacterial skin solutions prepared in Examples 1-3 and Comparative Examples 1-5 ; Table 2. Performance testing of the antibacterial skin solutions prepared in Examples 1-3 and Comparative Examples 1-5 ; Table 3. Performance testing of the antibacterial skin solutions prepared in Examples 1-3 and Comparative Examples 1-5 ; Skin barrier repair assay: Mice were used in the experiment. The temperature was 22±2℃ and the relative humidity was 50±5%. Mice were acclimatized to the environment 30 minutes in advance. The test site was the inner forearm of mice. A barrier damage model was established: repeated peeling of tape / stimulation with 0.5% sodium lauryl sulfate (SLS) caused a significant increase in transepidermal water loss (TEWL), simulating damaged sensitive skin. The initial TEWL of damaged skin was 35.2 g / (h·m). 2 The initial moisture content of the damaged skin stratum corneum was 22.5 au. Test period: The skin antibacterial solution prepared above was applied to the damaged area and used continuously for 14 days, twice a day. The TEWL and moisture content of the skin stratum corneum after using the antibacterial solution were measured (the better the hydration of the stratum corneum, the better the barrier function is improved; the lower the TEWL value, the better the barrier integrity, the less moisture loss, and the better the barrier repair effect).

[0048] The test results are shown in Table 4 below.

[0049] Table 4. Performance testing of the antibacterial skin solutions prepared in Examples 1-3 and Comparative Examples 1-5 ; As can be seen from the data in Tables 1, 2, 3 and 4, the antibacterial skin solutions prepared in Examples 1-3 have strong antibacterial effect, long-lasting antibacterial effect, are mild and non-irritating, and can repair the skin barrier, achieving long-lasting skin antibacterial effect, reducing the recurrence of skin inflammation, and alleviating inflammatory skin aging.

[0050] Comparative Example 1: When selenium-enriched chickpea sprout extract was replaced with a complex plant extract containing nanocrystals prepared from chickpea sprout extract and added to the skin antibacterial solution, its performance decreased. Chickpea sprout extract can inhibit the degradation of skin collagen and elastin while promoting collagen regeneration, repairing the skin barrier and improving skin elasticity. This allows the skin antibacterial solution to have both antibacterial care and anti-aging repair effects, reducing skin inflammation, redness, itching, and acne scar pigmentation caused by frequent use of antibacterial solution, thereby reducing skin oxidative damage. On the other hand, selenium-enriched chickpea sprout extract increases the protein content in the hydrolysate. The protein in the hydrolysate has good antioxidant activity, can enhance collagen synthesis and promote fibroblast proliferation, repair the skin barrier, achieve long-lasting skin antibacterial effect, reduce the recurrence of skin inflammation, and alleviate inflammatory skin aging.

[0051] Comparative Example 2 showed that when phlorizin was replaced by selenium-enriched chickpea sprout extract, a composite plant extract containing nanocrystals was added to the skin antibacterial solution. The performance of the solution decreased, demonstrating that phlorizin has antioxidant and free radical scavenging effects. Furthermore, as a lipophilic compound, phlorizin can alter the membrane dipole potential of the skin phospholipid bilayer, affecting the interfacial structure of the phospholipid bilayer and the water flow dynamics driven by water permeation. This allows intercellular lipids to penetrate the skin. Phlorizin, as a medium, can increase the transdermal effect of the composite plant extract containing nanocrystals, promoting its penetration and absorption on the skin and achieving a long-lasting antibacterial effect.

[0052] Comparative Example 3, in which rhubarb extract was replaced by licorice extract, and Comparative Example 4, in which licorice extract was replaced by angelica extract, prepared composite plant extracts containing nanocrystals, were added to the skin antibacterial solution. The performance of these extracts decreased, demonstrating that the plant extracts, including rhubarb extract, licorice extract, and angelica extract, contain anthraquinone compounds, anthrone compounds, tannins, and other chemical substances. These extracts have inhibitory effects on bacteria such as Escherichia coli and Staphylococcus aureus, and also possess certain anti-inflammatory effects. Furthermore, licorice extract can enhance the inhibitory effect of rhubarb extract on Escherichia coli and Staphylococcus aureus. In addition, angelica extract promotes transdermal absorption. The combination of these three extracts achieves broad-spectrum antibacterial, reduced irritation, and long-lasting transdermal effects.

[0053] Comparative Example 5 showed that replacing the composite plant extract containing nanocrystals with a mixed extract in the skin antibacterial solution resulted in a decrease in performance. This demonstrates that the composite plant extract containing nanocrystals, formed by mixing selenium-enriched chickpea sprout extract, plant extracts, and phloretin as the oil phase and hydroxypropyl methylcellulose as the aqueous phase, has a small size effect, which is beneficial for skin absorption and enables slow penetration into the superficial epidermis. This upgrades the antibacterial effect from surface to long-lasting, superficial antibacterial action, addressing the problem that ordinary antibacterial solutions only stay on the skin surface and cannot improve superficial flora imbalance. Furthermore, hydroxypropyl methylcellulose can be adsorbed on the crystal surface, providing a steric hindrance effect and inhibiting crystal aggregation. In addition, the plant extracts can be encapsulated inside the nanocrystals, which can improve the stability of the antibacterial solution.

[0054] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0055] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, they should all fall within the protection scope of the present invention.

Claims

1. A skin antibacterial liquid containing plant extracts, characterized in that, The ingredients include the following parts by weight: 15-20 parts of a complex plant extract containing nanocrystals, 3-4 parts of glycerin, 0.1-0.2 parts of sodium hyaluronate, and 75-85 parts of purified water; The composite plant extract containing nanocrystals is obtained by mixing selenium-enriched chickpea sprout extract, composite plant extract and phloretin as the oil phase, hydroxypropyl methylcellulose as the aqueous phase, and the oil phase and aqueous phase are mixed. The selenium-enriched chickpea sprout extract is specifically obtained by: cultivating chickpeas in a selenium-enriched culture to obtain selenium-enriched chickpea sprouts, and then extracting the product from them to obtain the selenium-enriched chickpea sprout extract.

2. The antibacterial skin solution containing plant extracts according to claim 1, characterized in that, The composite plant extract containing nanocrystals is prepared by the following steps: A1. After washing chickpea seeds with sodium hypochlorite solution, wash them with deionized water to completely remove the sodium hypochlorite solution, and obtain cleaned chickpea seeds. Add the cleaned chickpea seeds and sodium selenite to deionized water and hydrate them at 23-27℃ for 5-6 hours. Collect the hydrated seeds and place them in an incubator for germination to obtain selenium-enriched chickpea sprouts. A2. After freeze-drying selenium-enriched chickpea sprouts, grind them in a grinder and sieve them to obtain selenium-enriched chickpea sprout powder. Add the selenium-enriched chickpea sprout powder to hexane and stir at 45-55℃ for 12-13 hours. After filtration, place it in deionized water, adjust the pH with sodium hydroxide solution, stir, and then centrifuge. Collect the supernatant a and powder pellets. Mix the powder pellets with deionized water, stir, and centrifuge again to obtain supernatant b. Mix supernatant a and supernatant b to obtain selenium-enriched chickpea sprout extract. A3. Add selenium-enriched chickpea sprout extract, compound plant extract and phloretin to ethanol and stir well to form the oil phase. Add hydroxypropyl methylcellulose to deionized water to form the aqueous phase. Mix the oil phase and aqueous phase and stir at 1000-1500 r / min for 2-5 min. Remove the ethanol by vacuum rotary evaporation to obtain the compound plant extract containing nanocrystals.

3. The antibacterial skin solution containing plant extracts according to claim 2, characterized in that, In step A1, the mass ratio of the cleaned chickpea seeds, sodium selenite, and deionized water is 1:(0.01-0.03):(80-90).

4. The antibacterial skin solution containing plant extracts according to claim 2, characterized in that, In step A2, the mass ratio of the selenium-enriched chickpea sprout powder, hexane, and deionized water is 1:(6-10):(20-30).

5. A skin antibacterial liquid containing plant extracts according to claim 2, characterized in that, In step A2, the mass ratio of the powder spheres to deionized water is 1:(20-30).

6. The antibacterial skin solution containing plant extracts according to claim 2, characterized in that, In step A3, the mass ratio of the selenium-enriched chickpea sprout extract, the compound plant extract, the phloretin, and the ethanol is 1:(2-2.5):(0.3-0.5):(15-20).

7. The antibacterial skin solution containing plant extracts according to claim 2, characterized in that, In step A3, the mass ratio of hydroxypropyl methylcellulose to deionized water is 1:(100-120).

8. A skin antibacterial liquid containing plant extracts according to claim 2, characterized in that, In step A3, the mass ratio of the oil phase to the water phase is 1:(4-4.5).

9. A skin antibacterial liquid containing plant extracts according to claim 1, characterized in that, The compound plant extract is composed of rhubarb extract, licorice extract and angelica extract in a mass ratio of (8-10):(8-10):1; The pH of the skin antibacterial solution is 5.5-6.

0.

10. A method for preparing a skin antibacterial liquid containing plant extracts as described in any one of claims 1-9, characterized in that, The preparation steps include the following: Mix the composite plant extract containing nanocrystals, glycerin, sodium hyaluronate and purified water, stir at 80-100 r / min for 20-40 min, and sterilize at 70-75℃ for 25-30 min to obtain the skin antibacterial solution.