Preparation method, product and application of horseshoe crab peptide cream

A technology of limulus peptide paste and animal protease, which is applied in the fields of application, food science, animal feed, etc., can solve the problems of reducing feed intake, anorexia of experimental mice, and affecting the stability and reliability of experiments, so as to achieve convenient implementation and reduce Pollution, the effect of easy large-scale production

Active Publication Date: 2018-02-16
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Under normal experimental conditions, the full-price nutrient pellet feed can guarantee the above-mentioned feeding requirements, but the special feeds that need to be fed to model animals such as diabetic rats, coronary heart disease rats and hyperlipidemia rats often cause the experimental rats to have anorexia and reduce feed intake amount, which in turn affects the stability and reliability of the experiment

Method used

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  • Preparation method, product and application of horseshoe crab peptide cream
  • Preparation method, product and application of horseshoe crab peptide cream
  • Preparation method, product and application of horseshoe crab peptide cream

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: preparation limulus peptide cream

[0033] (1) The cleaned 5.4 kilograms of horseshoe crabs are cut into 5cm×5cm blocks, sent into the meat harvester to collect meat and seeds, and 3.5 kilograms of meat and seeds are taken;

[0034] (2) 3.5 kilograms of meat and seeds are added to a colloid mill and a small amount of water is obtained to obtain a fine slurry with a fineness of 150 meshes and a concentration of 20%;

[0035] (3) Adjust the pH of the fine slurry to 6.8-7.2 with phosphoric acid, add 180 mg of animal protease and 90 mg of flavor protease, and control the hydrolysis temperature to 53°C for 4 hours.

[0036] (4) After the hydrolysis, heat the temperature to 80°C to kill the enzyme for 10 minutes;

[0037] (5) Concentrate the enzymatic hydrolyzate in vacuum to a water content of 20% to obtain 1 kg of Limulus peptide paste product, which is sealed and packaged.

Embodiment 2

[0038] Embodiment 2: preparation limulus peptide paste

[0039] (1) The cleaned 4.2 kilograms of horseshoe crabs are cut into 5cm×5cm blocks, sent into the meat harvester to collect meat and seeds, and 3.2 kilograms of meat and seeds are taken;

[0040] (2) 3.2 kilograms of meat and seeds are added to a colloid mill and a small amount of water is obtained to obtain a fine slurry with a fineness of 180 meshes and a concentration of 16%;

[0041] (3) Adjust the pH of the fine slurry to 6.8-7.2 with phosphoric acid. Add 160mg animal protease and 80mg flavor protease, control the hydrolysis temperature to 55°C, and the time is 4h.

[0042] (4) After hydrolysis, heat the temperature to 85°C to kill the enzyme for 12 minutes;

[0043] (5) Concentrate the enzymatic hydrolyzate in vacuum to a water content of 35%, to obtain 1 kg of Limulus peptide paste product, which is sealed and packaged.

Embodiment 3

[0044] Embodiment 3: preparation limulus peptide paste

[0045] (1) Cut the cleaned 4.6 kg horseshoe crab into 5cm×5cm blocks, send them into the meat harvester to collect the meat and seeds, and take 3.3 kg of the meat and seeds;

[0046] (2) 3.3 kilograms of meat and seeds are added to a colloid mill and a small amount of water is obtained to obtain a fine slurry with a fineness of 130 meshes and a concentration of 24%;

[0047] (3) Adjust the pH of the fine slurry to 6.8-7.2 with phosphoric acid. Add 170mg animal protease and 85mg flavor protease, control the hydrolysis temperature to 56°C, and the time is 4h.

[0048] (4) After hydrolysis, heat the temperature to 90°C to kill the enzyme for 8 minutes;

[0049] (5) Concentrate the enzymatic hydrolyzate in vacuum to a moisture content of 10%, to obtain 1 kg of Limulus peptide paste product, which is sealed and packaged.

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Abstract

The invention relates to a preparation method, a product and an application of horseshoe crab peptide cream. The preparation method of the horseshoe crab peptide cream comprises the steps as follows:1) meat liquids and eggs of horseshow crabs are collected and pulped by a colloid mill, and fine pulp is obtained; 2) animal protease and flavor enzyme are added to the fine pulp for hydrolysis, heating for enzyme deactivation is conducted after hydrolysis ends, and an enzymatic hydrolysate is obtained; and 3) the enzymatic hydrolysate is subjected to vacuum concentration and the horseshoe crab peptide cream is obtained. The horseshoe crab peptide cream prepared with the preparation method can be used as an attractant, the ingestion desire of laboratory rats can be increased, the food intake of the laboratory rats can be guaranteed, and the molding quality of animal models can be improved.

Description

technical field [0001] The invention relates to the field of preparation of experimental animal feed additives, in particular to a preparation method of Limulus peptide cream and its product and application. Background technique [0002] Experimental mice mainly include rats and mice. Experimental mice play a very important role in animal experiments in the fields of toxicology, pharmacology, and drug efficacy. There are many advantages of using mice to do experiments. First, the DNA difference between experimental mice and humans is very small, and they have a close relationship, which has very important guiding significance for later clinical experiments; second, experimental mice and humans have many common diseases. It is convenient to make models; finally, the experimental mice have strong reproductive ability, fast sexual maturity, large number of reproduction, sufficient sources, and low price. [0003] In the feeding process of mice and mice, they are generally fed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/20A23K20/147A23K50/50A01K67/02A23L33/18
CPCA01K67/02A01K2267/03A01K2267/0362A01K2267/0375A23V2002/00A23K10/20A23K20/147A23K50/50A23L33/18A23V2200/326A23V2200/328
Inventor 陈颖由振强杨红忠宣尧仙郑高利
Owner ZHEJIANG ACAD OF MEDICAL SCI
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