26 results about "Horseshoe crab" patented technology

A reagent for β-glucan measurement without requiring a horseshoe crab hemocyte extract by using horseshoe crab-derived factor D in the reagent for β-glucan measurement.

This invention provides a recombinant horseshoe crab three-factor composition and its application. The composition comprises recombinant horseshoe crab factor C, recombinant horseshoe crab factor B, and recombinant horseshoe crab coagulase factor; all three factors are expressed by mammalian HEK293 cells. This allows for the expression of factors C, B, and PCE in mammalian HEK293 cells. Simultaneous expression of all three factors enhances factor C activity and improves sensitivity; furthermore, the expressed factors are secretible and possess high activity.

The utility model discloses a tachypleus amebocyte lysate sample adding device which comprises a base, a temperature control module arranged in the base, a plurality of storage grooves formed in the top end of the base, V-shaped bottom ends of the storage grooves, sample adding grooves formed in the storage grooves, covers arranged at the top ends of the sample adding grooves, and a plurality of sample adding holes formed in the top ends of the covers. The problems that waste is easily caused due to the fact that an existing sample adding device cannot conduct sampling completely, and an existing sampling device cannot be directly applied to automation equipment are solved.

The present invention relates to the field of biotechnology, and in particular to a Chinese horseshoe crab TaTrs_ALF_α1 antimicrobial peptide and a preparation method and application thereof. The present invention studies a horseshoe crab antimicrobial peptide: TaTrs_ALF_α1 for Chinese horseshoe crabs. It has been verified that the antimicrobial peptide has good inhibitory effects on common pathogenic bacteria in aquaculture: Staphylococcus aureus, Micrococcus luteus, Aeromonas hydrophila and / or Pseudomonas stutzeri. At the same time, it also has a good growth inhibition effect on gastric cancer cells SGC-7901 and / or esophageal cancer cells Eca-109. When used as a drug for treating esophageal cancer, the inhibitory effect on esophageal cancer cells Eca-109 can reach more than 90% when the concentration reaches 80 μg / mL, and the anticancer effect is good. Therefore, the antimicrobial peptide of the present application can be widely used in the fields of drug research and development and animal breeding immunoenhancers, and is a rare antimicrobial peptide.

To provide a method for producing a horseshoe crab recombinant Factor. The horseshoe crab recombinant Factor is produced through expression thereof by use of HEK293 as host cells.

The invention discloses a recombinant tachypleus amebocyte lyophilized preparation for bacterial endotoxin detection as well as a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is obtained by lyophilizing a lyophilized solution, wherein the lyophilized solution comprises the following components: mannitol, cane sugar, polyvinylpyrrolidone, recombinant human albumin, ascorbic acid, sodium chloride, inositol, L-arginine hydrochloride, ethylene glycol diethyl ether diamine tetraacetic acid, Tween 80, Proclin 300, Tris-HCl with the pH value of 7.0-8.0, a recombinant tachypleus amebocyte lyophilized preparation, a chromogenic substrate and a reaction buffer solution. The invention further discloses a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is prepared by matching a multi-element synergistic protection system with a seven-stage gradient temperature control freeze-drying process, and can be used for high-sensitivity and high-stability detection of bacterial endotoxin in drugs, medical instruments, biological products and clinical samples.

The invention presents a novel feed formulation and processing optimized for horseshoe crabs in prolonged captivity, with application to myriad species. The formulation is not only sustainable but environmentally advantageous at scale by utilizing fish and poultry processing by-products. The nutrient-rich waste products are enhanced through cold-processed, enzymatic liquefication and fermentation, which preserves proteins and vitamins and thus, nutrient integrity and bioavailability. The formulation is matched to the composition of the horseshoe crab and the flora and fauna of its habitat diet. Feed efficacy can be measured by observed attraction and palatability, as well as parameters including but not limited to cellular integrity and amebocyte reactivity, weight, disease resistance, and carapace metrics. The feed matrix is designed to stimulate benthic foraging and feeding behaviors of horseshoe crabs but can be matched to alternative species feeding behaviors in aquaculture, agriculture, and other captivity settings, including nourishment of companion animals.

The invention discloses a bacterial biosensor as well as a preparation method and application thereof. According to the bacterial biosensor, glass (BK7) with the surface covered with gold nanoparticles serves as a substrate, the surfaces of the gold nanoparticles are coupled and modified with specific antibodies, and the gold nanomaterial is prepared and applied to detection of bacterial endophytic ophthalmia. Gold nanoparticles are taken as a substrate, a high-sensitivity, selective and label-free method for simultaneously detecting gram-positive (G +) and gram-negative (G-) bacteria in a sample is constructed, small sample size requirements and short experiment operation time are met, and when the method is used for detecting the bacteria, the use of tachypleus amebocyte lysate can be avoided; the method has the advantages that the method is simple and convenient to operate, can effectively distinguish G < + > and G <-> bacteria, is more efficient to use, is a more humanized and ecologically sustainable bacteria detection method, plays an important role in protecting the horseshoe crabs, and is convenient to popularize and use.

A reagent for β-glucan measurement without requiring a horseshoe crab hemocyte extract by using horseshoe crab-derived factor D in the reagent for β-glucan measurement.

The application discloses a Chinese horseshoe crab breeding method and belongs to the technical field of aquaculture. The Chinese horseshoe crab breeding method is characterized by the following steps: selecting Chinese horseshoe crab seedlings and placing the seedlings in a culture box, and laying a breeding substrate at the bottom of the culture box, wherein the breeding substrate comprises sediments collected from a natural habitat of the Chinese horseshoe crab. The breeding method can promote the development of Chinese horseshoe crab seedlings, especially the growth and development of diapause 1-year-old Chinese horseshoe crabs, and improve the molting rate and survival rate of the Chinese horseshoe crabs.

The invention relates to the technical field of gene engineering, in particular to a horseshoe crab recombinant factor C based on human cell expression and application of the horseshoe crab recombinant factor C. The codon of the horseshoe crab recombinant factor C is shown as a sequence ID 1. The horseshoe crab recombinant factor C disclosed by the invention can specifically recognize and combine with endotoxin, sensitive and accurate detection of the endotoxin is realized by constructing a fluorescence reaction system, and compared with a traditional horseshoe crab blood reagent, the horseshoe crab recombinant factor C used by the invention has the advantages of controllable preparation, lower cost, no animal ethical dispute and the like. In addition, the horseshoe crab recombinant factor C prepared on the basis of a human cell expression system has better stability and is suitable for endotoxin detection in various complex samples.

The present invention relates to “a heterodimer which combines a Factor G α-subunit containing an amino acid sequence that is the same as or substantially the same as an amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4 with a Factor G β-subunit containing an amino acid sequence that is the same as or substantially the same as an amino acid sequence represented by any one of SEQ ID NO: 6, 8, 10, 12, 14, or 16, a method of measuring a β-glucan using the heterodimer, and a kit for measuring a β-glucan containing the heterodimer”.

The invention relates to the technical field of biological engineering and aquaculture, and discloses a horseshoe crab egg air bath incubation method which comprises the following steps: S1, constructing a multi-layer incubation frame, arranging incubation basins on each layer, slightly inclining the incubation basins, forming openings in the bottoms of the lower ends of the incubation basins, connecting drainage pipes for draining water, and mounting water spray pipes above the incubation basins; s2, a net disc is placed in the hatching basin, a water-absorbing non-woven fabric is placed on the net disc, and horseshoe crab eggs are evenly distributed on the wet non-woven fabric; s3, continuously or alternately spraying the hatching basins on each layer of the hatching frame through a continuous spraying system, and maintaining proper humidity. Through the independent temperature and humidity regulation and control units, the accuracy and response speed of environment control are improved, waste of energy and water resources is effectively reduced through an automatic temperature and humidity management system, sanitation and safety of incubation equipment are improved through cleaning and disinfection measures, the pollution risk of pathogens is reduced, and therefore the incubation efficiency and the success rate are improved.

The utility model provides a Chinese tachypleus tridentatus oviposition collector, which relates to the technical field of Chinese tachypleus tridentatus cultivation, and comprises a collecting shovel, a connecting sleeve and a collecting cylinder, the inner side of the collecting shovel is provided with an inlet, the lower surface of the collecting shovel is symmetrically and fixedly provided with universal wheels, and the surface of the collecting shovel is fixedly provided with a push rod. A connecting pipe is fixedly connected to the side face of the collecting shovel, a drainage groove is formed in the surface of the collecting barrel, a collecting net is slidably connected into the collecting barrel, and a mounting plate is fixedly mounted on the surface of the connecting pipe. According to the horseshoe crab egg collecting device, the limiting rod is pulled to slide out of the connecting sleeve, at the moment, the collecting barrel is grabbed and pulled, then the collecting barrel can be separated from the connecting pipe, when the collecting barrel is full of horseshoe crab eggs, workers are allowed to rapidly empty the collecting barrel through the design of rapid disassembly, the waiting time and the manual disassembly time are shortened, and the working efficiency is improved. Therefore, more tachypleus tridentatus eggs can be treated in a short time, and the overall working efficiency is improved.

The invention discloses a recombinant tachypleus tridentatus C factor based on codon optimization and application of the recombinant tachypleus tridentatus C factor in endotoxin detection, and relates to the technical field of bioengineering. The limulus C factor sequence is used as a starting sequence, the limulus C factor is recoded, and the gene sequence of the recombinant limulus C factor is shown as SEQ ID NO: 1; protein corresponding to the sequence is expressed through an insect cell-baculovirus expression system, and the expression quantity is high, that is, the protein expression quantity of the recombinant horseshoe crab factor C is successfully improved through codon optimization. The production cost of the recombinant tachypleus tridentatus C factor endotoxin detection method kit is reduced, and the recombinant tachypleus tridentatus C factor detection method replaces a tachypleus tridentatus reagent detection method to become a mainstream method for endotoxin detection, so that the problem of raw material supply of tachypleus tridentatus reagent due to the fact that tachypleus tridentatus becomes a protective animal is solved; and the existing recombinant horseshoe crab factor C detection method has the problems of high product price and the like.

The utility model discloses a measuring scale used for contrasting and measuring the widths of tachypleus tridentatus ages and the width of a carapace, relates to the technical field of tools for measuring the tachypleus tridentatus ages, and solves the problem that an existing graduated scale is poor in effect of measuring the widths of the tachypleus tridentatus ages and the width of the carapace. According to the technical scheme, the ruler comprises a ruler body, a fixed claw arranged at the lower end of the ruler body and a moving claw, the fixed claw is arranged at the front end of the ruler body and is aligned with the side edge of the ruler body, and the moving claw slides back and forth relative to the ruler body; the front surface of the ruler body is a contrast area, a first scale for marking the age of the horseshoe crabs and a second scale for marking the width of the carapace of the horseshoe crabs are arranged on the contrast area of the ruler body, and a plurality of contrast grooves are formed in the contrast area of the ruler body. The tachypleus tridentatus age is obtained through comparison, the tachypleus tridentatus age can be directly obtained without looking up data after the tachypleus tridentatus width is measured, the tachypleus tridentatus age measuring device has the advantages of being convenient to carry and operate, meanwhile, the tachypleus tridentatus cannot be damaged, and working efficiency is improved.

The present invention relates to a method for enhancing a sensitivity of an endotoxin measuring reagent to the endotoxin of Helicobacter pylori, the endotoxin measuring reagent comprising a recombinant protein of horseshoe crab factor C, a recombinant protein of horseshoe crab factor B, and a recombinant protein of horseshoe crab pro-clotting enzyme, the method comprising increasing a content of the recombinant protein of factor C to 162 ng / mL or more at the time of endotoxin measurement.

Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by combining this polypeptide with horseshoe crab factor C, as a Limulus reagent.

An object of the present invention is to provide a high-performance reagent for β-glucan measurement without requiring a horseshoe crab hemocyte extract. The invention can provide a high-performance reagent for β-glucan measurement without requiring a horseshoe crab hemocyte extract by using horseshoe crab-derived factor D in the reagent for β-glucan measurement.

The invention discloses a wide-temperature-range compound enzyme endotoxin detection reagent and a preparation method thereof. According to the reagent, an active enzyme compound in lysate of limulus blood cells serves as a core, a temperature control response material, a modifier and a stabilizer are compounded to construct an intelligent compound enzyme system, the narrow temperature range limitation of a traditional reagent is broken through, the activity can be accurately adjusted within the range of 0-90 DEG C, the relative activity at the temperature of 20-70 DEG C is kept at 80-95%, and the relative activity at the temperature of 80-90 DEG C is improved to 120-130% of the initial activity. The detection limit of the reagent is 0.005-0.02 EU / mL, the activity retention rate at the temperature of 50 DEG C is increased by 75%-90% compared with that of a traditional tachypleus amebocyte lysate, and the reagent is excellent in stability in a high-salt and low-pH environment, adapts to temperature fluctuation scenes and is suitable for endotoxin detection of drugs, food and medical instruments.

The present invention designs an underwater working robot, specifically disclosing a bionic horseshoe crab vector-propelled robot, comprising a front shell and a rear shell that are movably connected, a vector longitudinal adjustment component being provided between the front shell and the rear shell, a stepping direction adjustment component being relatively provided at the bottom of the front shell, a side wing penetrating the front shell being provided at the top of the inner side of the front shell, a vertex support wheel and a folding suspension wheel being provided at the bottom of the front shell for supporting the robot to contact the ground, and a vector nozzle assembly being provided on the inner side of the front shell and the rear shell. The bionic horseshoe crab vector-propelled robot, in which the front shell and the rear shell are combined to form the streamlined shape of the horseshoe crab, replicates the low-resistance form that the horseshoe crab has evolved in nature over hundreds of millions of years, and can effectively reduce the pressure difference resistance and friction resistance during underwater movement. Compared with traditional multi-propeller robots, its appearance design reduces useless energy consumption, and can significantly improve endurance, especially in complex water flow environments.

This invention discloses a method for surveying juvenile horseshoe crabs in China based on remote sensing technology and intelligent image recognition. Through a process of rapid comprehensive survey and precise regional survey, a drone equipped with a lightweight YOLO recognition model, RTK positioning, and laser ranging is used to conduct a comprehensive survey of potential habitats, identify juvenile distribution hotspots, and delineate precise survey areas. Then, a full-frame mapping camera is used to conduct ultra-high-resolution drone photogrammetry, combined with an instance segmentation model to complete juvenile contour extraction, coordinate positioning, density statistics, and age determination. This invention overcomes the shortcomings of traditional foot surveys, such as tidal limitations, small coverage area, susceptibility to environmental interference, high missed detection rate, and high personnel risk. It achieves non-contact, high-efficiency, high-precision, and low-disturbance juvenile horseshoe crab surveys, providing a scientific and efficient technical solution for the monitoring of juvenile horseshoe crabs in China and the protection of endangered species.

Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by combining this polypeptide with horseshoe crab factor C, as a Limulus reagent.

The utility model relates to an artificial tachypleus tridentatus house which comprises a reinforced concrete box body of a net-shaped empty box structure, the box body comprises a top plate and a bottom plate, the top plate is arranged above the bottom plate, the middle of the bottom plate is hollowed out, square columns are arranged at the four end corners and the middle positions of the side edges of the bottom plate, and the square columns are connected with the top plate and the bottom plate. A cross-shaped supporting rod is arranged at the hollowed-out position of the bottom plate, and holes distributed in an array mode are formed in the top plate. According to the utility model, the structure is stable, the resistance is small, and the drifting risk is low.

The invention relates to an underwater operation robot, and particularly discloses a bionic tachypleus tridentatus vector pushing robot which comprises a front shell and a rear shell which are movably connected, a vector longitudinal adjusting assembly is arranged between the front shell and the rear shell, and stepping direction adjusting assemblies are oppositely arranged at the bottom of the front shell. The top of the inner side of the front shell is provided with side wings penetrating through the front shell, the bottom of the front shell is further provided with vertex supporting wheels and folding suspension wheels which are used for supporting the robot to make contact with the ground, and the inner side of the front shell and the inner side of the rear shell are provided with thrust vectoring nozzle assemblies. According to the bionic tachypleus tridentatus vector pushing robot, the front shell and the rear shell are combined to form the streamline shape of the tachypleus tridentatus, the low-resistance form of the tachypleus tridentatus evolved for hundreds of millions of years in the nature is rescribed, and differential pressure resistance and friction resistance during underwater movement can be effectively reduced. Compared with a traditional multi-propeller robot, useless energy consumption is reduced through the appearance design of the multi-propeller robot, and the cruising ability can be remarkably improved especially in the complex water flow environment.

The present invention discloses a cultivation method for improving the molting rate and survival rate of diapause first-instar Chinese horseshoe crabs. The diapause first-instar Chinese horseshoe crabs refer to individuals that are hatched into first-instar juveniles in September of the current year and have not molted into second-instar horseshoe crabs until April of the following year. The method comprises the following steps: selecting diapause first-instar Chinese horseshoe crabs and placing them in seawater containing 30-90 mg / L of vitamin C for cultivation. The present invention can not only effectively improve the molting rate of diapause first-instar juvenile horseshoe crabs but also improve the survival rate of the juvenile horseshoe crabs, thereby obtaining second-instar horseshoe crab seedlings with good quality.