4 results about "Horseshoe crab" patented technology

To provide a method for producing a horseshoe crab recombinant Factor. The horseshoe crab recombinant Factor is produced through expression thereof by use of HEK293 as host cells.

This invention discloses a method for surveying juvenile horseshoe crabs in China based on remote sensing technology and intelligent image recognition. Through a process of rapid comprehensive survey and precise regional survey, a drone equipped with a lightweight YOLO recognition model, RTK positioning, and laser ranging is used to conduct a comprehensive survey of potential habitats, identify juvenile distribution hotspots, and delineate precise survey areas. Then, a full-frame mapping camera is used to conduct ultra-high-resolution drone photogrammetry, combined with an instance segmentation model to complete juvenile contour extraction, coordinate positioning, density statistics, and age determination. This invention overcomes the shortcomings of traditional foot surveys, such as tidal limitations, small coverage area, susceptibility to environmental interference, high missed detection rate, and high personnel risk. It achieves non-contact, high-efficiency, high-precision, and low-disturbance juvenile horseshoe crab surveys, providing a scientific and efficient technical solution for the monitoring of juvenile horseshoe crabs in China and the protection of endangered species.

Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by combining this polypeptide with horseshoe crab factor C, as a Limulus reagent.

The present invention discloses a cultivation method for improving the molting rate and survival rate of diapause first-instar Chinese horseshoe crabs. The diapause first-instar Chinese horseshoe crabs refer to individuals that are hatched into first-instar juveniles in September of the current year and have not molted into second-instar horseshoe crabs until April of the following year. The method comprises the following steps: selecting diapause first-instar Chinese horseshoe crabs and placing them in seawater containing 30-90 mg / L of vitamin C for cultivation. The present invention can not only effectively improve the molting rate of diapause first-instar juvenile horseshoe crabs but also improve the survival rate of the juvenile horseshoe crabs, thereby obtaining second-instar horseshoe crab seedlings with good quality.