Artificial breeding and culturing method for horseshoe crabs
A breeding method and artificial technology, applied in the field of artificial breeding and breeding, can solve problems such as the inability to apply artificial breeding, and achieve huge economic and social benefits
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[0049] The following examples further illustrate the present invention.
[0050] 1. Artificial breeding
[0051] 1. The choice of pro-Limulus
[0052] The key to the success of artificial insemination is to choose a good horseshoe crab with good maturity. Scrub the male and female horseshoe crabs with sea water.
[0053] Open the reproductive cover of the abdomen of the female horseshoe crab, and a pair of reproductive holes can be seen. Squeeze the reproductive holes with hands. If mature eggs are discharged, the female horseshoe crab can be retained for insemination.
[0054] Open the genital cover of the male Limulus abdomen, use a syringe to extract hemolymph (containing semen) from the genital opening, drop it on the glass slide, add the same amount of seawater, cover the glass slide, and observe under a high-power microscope to confirm that there are a large amount of blood. The swimming sperm, the male horseshoe crab can be retained. If no motile sperm can be observ...
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