Proliferation culture medium and proliferation method for large-flower-like honeysuckle flower Yulei #1

A proliferation medium and proliferation culture technology, which is applied in the field of proliferation medium of Lonicera Lonicerae Yulei No. 1, can solve the problems of low survival rate of cuttings, slow promotion, low reproduction coefficient, etc.

Inactive Publication Date: 2015-05-27
CHONGQING UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are not many rootstock and scion resources for grafting, and the survival rate of cuttings is lower than 40%, resulting in low reproduction coefficient and slow promotion, which cannot meet production and market demand.

Method used

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  • Proliferation culture medium and proliferation method for large-flower-like honeysuckle flower Yulei #1
  • Proliferation culture medium and proliferation method for large-flower-like honeysuckle flower Yulei #1
  • Proliferation culture medium and proliferation method for large-flower-like honeysuckle flower Yulei #1

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Experimental program
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Embodiment Construction

[0010] Preferred embodiments of the present invention will be described in detail below.

[0011] The test material Lonicera tomentosa Yulei No. 1 explant used in the embodiment of the present invention was collected from Xiushan County, Chongqing City, and the sterile seedlings were provided by the tissue culture workshop of the Flower Research Institute of Chongqing University of Arts and Sciences.

[0012] 1. Screening of basic medium

[0013] Single buds were cut from the cluster buds induced by the primary culture of Lonicerae grisinica Yulei No. 1 tissue culture seedlings, cut into 1.0-1.2 cm long stem segments, and inoculated on MS, improved B5, WPM, and B5 as the basic medium. Bud proliferation was cultured in a medium supplemented with 1.0 mg / L of 6-benzylaminopurine (6-BA), 0.8 mg / L of indole acetic acid (IAA), 30 g / L of sucrose, and 6 g / L of agar. Eight bottles were inoculated for each treatment, and three basically identical explants were inoculated in each bottle...

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Abstract

The invention discloses a proliferation culture medium and a proliferation method for large-flower-like honeysuckle flower Yulei #1. According to the proliferation culture medium, a culture medium which is the most suitable for the proliferation of the large-flower-like honeysuckle flower Yulei #1 is as follows: on the basis of an improved B5 culture medium, the contents of (NH4)2SO4 and CaCl2 are adjusted to two times as much as previous contents and the content of MgSO4 is adjusted to 0.6 time as much as the previous content; the concentration of microelements is adjusted to 1 / 3 of the previous concentration; the concentration of organic matters is adjusted to 1 / 2 of the previous concentration; the concentration of an iron salt is adjusted to 1.5 times as much as the previous concentration; 1.0mg / L of 6-benayl aminopurine, 0.8mg / L of indoleacetic acid, 30g / L of sucrose and 6g / L of agar are added; in the proliferation process, the optical cutting process is that an induced bud is cut into a stem section with the length of 1cm-1.2cm, leaves are not removed and the upper section with the length of 2mm is remained. According to the proliferation culture medium and the proliferation method for the large-flower-like honeysuckle flower Yulei #1, a germchit industrial system of the large-flower-like honeysuckle flower Yulei #1 is established and the germchit problem of the large-flower-like honeysuckle flower is solved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a proliferation medium and a proliferation method of Lonicerae Lonicerae Yulei No. 1. Background technique [0002] Gray felted honeysuckle (Lonicera m acranthoides Hand.Mazz) is a perennial semi-evergreen winding small shrub or erect small shrub plant of Lonicera genus Lonicera. The content of chlorogenic acid in its flower buds is the highest among all kinds of honeysuckle in the country. It is a commodity in Southwest and Central South regions. One of the main varieties of honeysuckle. It contains volatile oils, flavonoids, triterpene saponins, organic acids and inorganic elements, etc. It has antibacterial, antiviral, antipyretic, anti-inflammatory, liver-protecting, and anti-AIDS effects. [0003] 'Yulei No. 1' is a variety of Lonicera chinensis. It was approved as a new variety by experts from the Chongqing Forest Variety Approval Committee in 2008. This variety...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈泽雄刘奕清黄登艳
Owner CHONGQING UNIV OF ARTS & SCI
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