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A method for preparing kynuric acid from Limulus chinensis

A technology of kynuric acid and Limulus tail, applied in the field of active medicine

Active Publication Date: 2018-04-24
JIMEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, it has not been reported that kynuric acid exists in Limulus sinensis

Method used

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  • A method for preparing kynuric acid from Limulus chinensis
  • A method for preparing kynuric acid from Limulus chinensis
  • A method for preparing kynuric acid from Limulus chinensis

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Embodiment Construction

[0033] The present invention discloses a method for preparing kynuric acid from Chinese horseshoe crab tail, including the following steps:

[0034] Step 1. Preparation of Chinese horseshoe crab tail dry powder: dry the Chinese horseshoe crab tail in the sun, pulverize it with a medicinal pulverizer at room temperature to obtain the dry powder, put it in a sealed bag, and store it in a desiccator for later use;

[0035] Step 2. Preparation of crude Chinese horseshoe crab tail: Weigh 5 parts of Chinese horseshoe crab tail that have been powdered, 1000g each, put them into 5 2L Erlenmeyer flasks, and add acetic acid according to the ratio of material to liquid 1:3:16 , Ethyl acetate and methanol, sealed and placed in a shaker, extracted at 40℃, 150rpm for 48h, filtered with suction, collected the filtrate; then added the corresponding solvent to the residue (according to the material-to-liquid ratio 1:3:16, add acetic acid, Ethyl acetate and methanol) for the second extraction. Under...

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Abstract

The invention discloses a method for preparing kynurenine from horseshoe crab tails.The method comprises the following steps of preparation of dry horseshoe crab tail powder, preparation of horseshoe crab tail crude extract, primary separation and purification of the horseshoe crab tail crude extract, separation and purification of a component A8 and separation and purification of components A8-24, wherein separation and purification of the components A8-24 comprise the steps that an appropriate amount of components A8-24 are taken, methyl alcohol is added for dissolution, and normal phase column separation is conducted; 50 mg of samples are taken and stirred with 150 mg of silica gel powder, 5 g of silica gel is saturated with petroleum ether and then subjected to column packing, after the the samples are packed in a column, elution is conducted with petroleum ether and acetone, when the ratio of petroleum ether to acetone is 1:10, fractions are collected, the number of the fractions is 60, the fractions from the 25 tube to the 40 tube are merged, after the the fractions are merged, vacuum concentration is conducted, and after solvent is dried out, kynurenine is obtained.According to the method for preparing kynurenine from the horseshoe crab tails, kynurenine is separated from the horseshoe crab tails for the first time, and kynurenine serving as the active ingredient of the horseshoe crab tails has great significance in secondary development of horseshoe crab tails.

Description

Technical field [0001] The present invention relates to the technical field of active drugs, in particular to a method for preparing kynuric acid from Chinese horseshoe crab tail. Background technique [0002] Kynurenine (Kyn) is one of the metabolites of the essential amino acid tryptophan (Trp). In addition to the synthesis of protein, Trp ingested by the human body is mainly metabolized in tissues and organs such as the liver, kidney, and brain to generate a variety of biologically active molecules with physiological effects, such as Kyn, serotonin, and quinolinic acid. Kyn and its metabolites are closely related to a variety of neuropsychiatric diseases, renal failure, cataracts, and various chronic malignant diseases. Therefore, Kyn has important reference value for the diagnosis and treatment of related diseases, curative effect monitoring and pathogenesis. In the prior art, it has not been reported that kynuric acid is present in Chinese horseshoe crab tail. [0003] In v...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D215/48
CPCC07D215/48
Inventor 李健苏文金刘鹏飞苏国成刘静雯王力
Owner JIMEI UNIV
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