Method for obtaining natural horseshoe crab fertilized eggs by building pool bottom overhead, laying sand and conducting partition

A technology of fertilized eggs and sand partitions, applied in the field of natural fertilized eggs, can solve problems such as low efficiency, achieve improved quality, ensure stability, and be conducive to egg laying and ovulation.

Active Publication Date: 2015-01-07
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this patented technology described in the current state of art lies on promoting growth of greenhouse crops without excessive water consumption or waste due to lack of adequate supply of nitrogen sources during early stages of their growing period. By adding certain substances like calcium hydroxide and magnesium oxaluminate to these areas, they promote root formation while preventing deaths caused by overexuberant roots. Additionally, it suggests creating artificial conditions called stomach nests within the plant's interior space where young shoots grow upward instead of being buried underground. These artificial spaces help maintain proper levels of carbon dioxide gas concentration inside the soil, resulting in better seedling survival rates. Overall, this innovative solution helps improve vegetable production processes such as germination, rotation of egg shells, enzymatic rearing, and nutritional enhancement methods.

Problems solved by technology

This patents discusses different techniques for studying horse shoe crab behavior during migration between seafood sources like Antarctica and Arctic Ocean. However, there exist technical problem addressed in these studies: The current practicality of laboratory study results in low numbers of horseshcrugs from natural populations due to their lack of food supply and potential harmful effects associated with them. Therefore, new technologies aimed towards developing better tools for understanding how horseshoebbles actively play role in regulating ocean environments.

Method used

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Embodiment 1

[0027] Embodiment 1: a kind of method of the present invention adopts overhead sanding partition at the bottom of the pool to obtain the natural fertilized egg of horseshoe crab, and it comprises the following steps:

[0028] a. Selection of pro-Limulus: At the beginning of June 2009, during the breeding season of Limulus in China, 2 pairs of pro-Limulus were selected as parents. After inspection, it was found that the ventral surface of the carapace was full, the body contained a large number of gametes, and the limbs were intact, strong and active; and the rear cover of the horseshoe crab was lifted, and a small amount of egg fluid was released by the female, and a small amount of egg fluid was released by the male. A small amount of semen comes out.

[0029] b. Indoor sanding and transformation of the Limulus pool: In order to further try the behavior of traditional cement pool sanding to obtain natural fertilized eggs, a 10m3 cement pool was selected to only sand 30cm, and...

Embodiment 2

[0035] Embodiment 2, a kind of method that adopts overhead sand-spreading partition at the bottom of the pool to obtain natural fertilized eggs of horseshoe crab of the present invention, it comprises the following steps:

[0036] a. Selection of pro-Limulus: At the beginning of June 2012, during the breeding season of Chinese Limulus, 5 pairs of pro-Limulus were selected as broods. After inspection, it was found that the ventral surface of the carapace was full, the body contained a large number of gametes, and the limbs were intact, strong and active; and the rear cover of the horseshoe crab was lifted, and a small amount of egg fluid was released by the female, and a small amount of egg fluid was released by the male. A small amount of semen comes out.

[0037] b. Indoor reconstruction of the horseshoe crab pool: use a wooden frame to lift the bottom of the 50m2 cement pool, the height of which is 12cm, spread 60-mesh sieve silk net, lay it flat and fix it, and put the infl...

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Abstract

The invention discloses a method for obtaining natural horseshoe crab fertilized eggs by building a pool bottom overhead, laying sand and conducting partition. The method is characterized by including the steps of firstly, selecting parent horseshoe crabs, wherein three or more pairs of parent horseshoe crabs are selected as the parents in the mating season each year; secondly, refitting a parent horseshoe crab pool indoors, wherein the pool bottom of a breeding pool is built overhead through a wood frame, a 60-mesh bolting-silk net is laid on the pool bottom, an oxygen pipe used for inflation is arranged in the overhead wood frame, and sand is laid on an egg laying area; thirdly, breeding the parent horseshoe crabs indoors, wherein the parent horseshoe crabs selected in the first step are placed in the parent horseshoe crab pool refitted in the second step, and indoor inflation breeding is conducted; fourthly, making the parent horseshoe crabs lay eggs and release semens, wherein the parent horseshoe crabs can lay eggs and release semens in 2 days to 10 days after entering the breeding season to form fertilized eggs; fifthly, sieving and washing the fertilized eggs; wherein after the fertilized eggs are hardened, the fertilized eggs and sand are dug out, elutriation is conducted through a screen with the diameter of 2 mm, all the sand is leaked out, and the fertilized eggs are left. Different from a method for obtaining the fertilized eggs by killing horseshoe crabs, the method has the advantages that a large number of high-quality fertilized eggs can be obtained, and the method is easy and convenient to operate, efficient, highly feasible and the like.

Description

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Claims

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Application Information

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Owner GUANGXI ACADEMY OF FISHERY SCI
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