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Horseshoe crab antiviral combined extract, preparation method and application of horseshoe crab antiviral combined extract to disinfection products

An extract, anti-virus technology, applied in disinfection products, Limulus anti-virus combination extract, preparation field, can solve the problem that chemical disinfectants cannot be directly sprayed on a large area, so as to avoid drug resistance and allergies , reduce production costs, and have no toxic and side effects

Active Publication Date: 2021-08-06
GENOBIO PHARM CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relevant technical documents that stipulate that chemical disinfectants cannot be directly sprayed on the human body in large areas.

Method used

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  • Horseshoe crab antiviral combined extract, preparation method and application of horseshoe crab antiviral combined extract to disinfection products
  • Horseshoe crab antiviral combined extract, preparation method and application of horseshoe crab antiviral combined extract to disinfection products
  • Horseshoe crab antiviral combined extract, preparation method and application of horseshoe crab antiviral combined extract to disinfection products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Limulus whole blood was collected, and Limulus blood cells and serum were separated.

[0039] Limulus blood cell lysis treatment: Use distilled water as the lysate, and gently shake the cells and the lysate at a ratio of 1:5. After repeated freezing and thawing, use a high-speed homogenizer for emulsification and separation, and the homogenization time is 5 minutes. Then, ultrasonic crushing is carried out again, and the crushing conditions are as follows: ultrasonic output power is 500W, ultrasonic pulse action time is 10s, interval is 10s, and cumulative action time is 10 minutes. So repeated ultrasound 3 times, a total of 30min. After the turbid solution was centrifuged at high speed, the supernatant was taken to obtain the crude extract of Limulus blood cells with antiviral effect, which was recorded as extract A1.

[0040]Treatment of Limulus serum: Slowly add saturated ammonium sulfate solution to Limulus serum until the saturation is 55%, stir while adding, and...

Embodiment 2

[0055] Limulus whole blood was collected, and Limulus blood cells and plasma were separated.

[0056] Limulus blood cell lysis treatment: Use distilled water as the lysate, and gently shake the cells and the lysate at a ratio of 1:5. After repeated freezing and thawing, use a high-speed homogenizer for emulsification and separation, and the homogenization time is 5 minutes. Then, ultrasonic crushing is carried out again, and the crushing conditions are as follows: ultrasonic output power is 500W, ultrasonic pulse action time is 10s, interval is 10s, and cumulative action time is 10 minutes. So repeated ultrasound 3 times, a total of 30min. The turbid liquid was subjected to high-speed centrifugation and the supernatant was taken to obtain the extract A2 with antiviral effect.

[0057] Treatment of Limulus serum: Slowly add saturated ammonium sulfate solution to Limulus serum until the saturation is 55%, stir while adding, and centrifuge at 8000rpm for 10min to separate the l...

Embodiment 3

[0064] Limulus whole blood was collected, and Limulus blood cells and plasma were separated. Limulus blood cells are first used in the production of Limulus reagent, and the lower sediment after the emulsion is centrifuged is used as a raw material for standby.

[0065] Lysis treatment of Limulus reagent waste: Use distilled water as the lysate, gently shake and mix the waste and lysate at a ratio of 1:5, and use a high-speed homogenizer for emulsification and separation after repeated freezing and thawing. The homogenization time is 20 minutes. Then, ultrasonic crushing is carried out again, and the crushing conditions are as follows: ultrasonic output power is 700W, ultrasonic pulse action time is 10s, interval is 10s, and the cumulative action time is 10min as one time. So repeated ultrasound 3 times, a total of 30min. The turbid liquid was subjected to high-speed centrifugation and the supernatant was taken to obtain the extract A3 with antiviral effect.

[0066] Treatme...

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Abstract

The invention discloses a horseshoe crab antiviral combined extract, a preparation method and an application of the horseshoe crab antiviral combined extract to disinfection products. Two extracts with antiviral effects are respectively obtained from horseshoe crab serum and horseshoe crab blood cells, and the extracts have more obvious inhibition effects on viruses when being combined for use. The novel coronavirus resisting activity of a spray dressing formed by combining the two extracts reaches up to 99.51%, and the influenza A virus resisting activity is 42.58%. The two extracts can be extracted from waste materials obtained from horseshoe crab reagent production, so that the production waste materials are recycled, and biological resources can also be fully utilized while the production cost is reduced. The two antiviral extracts can be prepared to obtain dressings for nasal cavity and skin disinfection. Compared with traditional chemical disinfection products, the two antiviral extracts are safer, milder and more effective, have no toxic and side effects, and can avoid drug resistance and allergy phenomena of conventional disinfection products.

Description

technical field [0001] The invention belongs to the field of biological preparations, and in particular relates to a limulus antiviral combination extract, a preparation method and its application in disinfection products. Background technique [0002] Commonly used chemical disinfectants, such as chlorine-containing disinfectant (84 disinfectant), alcohol disinfectant (75% alcohol), etc., can kill viruses, but they are dangerous chemicals and must be strictly followed the instructions Optional, when the concentration is too high, it can stimulate, damage the skin and mucous membranes, and corrode articles. Severe cases can lead to recurrence of chronic bronchitis in the elderly, asthma attacks in children; and overuse of chemical disinfectants can bring other risks. Long-term use of the same disinfectant in large quantities will cause microorganisms to develop drug resistance, and the sterilization effect will be greatly reduced. [0003] The main route of transmission of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/02A61K35/14A61K9/12A61P31/14A61P31/16A61P31/20A61P11/02A61P17/00C07K1/16C07K1/30C07K1/36
CPCA61K38/02A61K35/14A61K9/12A61K9/0043A61K9/0014A61P31/14A61P31/16A61P31/20A61P11/02A61P17/00C07K1/36C07K1/303C07K1/30C07K1/16A61K2300/00Y02A50/30
Inventor 张文倩何永胜张桐嘉许可薛懿臧丹戎刘萌萌李世林黄炎彬
Owner GENOBIO PHARM CO LTD
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