A method for obtaining natural fertilized eggs of Limulus japonicus by adopting the overhead sand-dressing partition at the bottom of the pool

Abstract

The invention discloses a method for obtaining natural fertilized eggs of horseshoe crabs by adopting overhead sand-spreading partitions at the bottom of the pool, which is characterized in that it comprises the following steps: a. selection of pro-Limulus: in the breeding season of Limulus each year, select more than 3 pairs of pro-Limulus As a parent; b. Indoor modification of the horseshoe crab pool: use a wooden frame to lift the bottom of the breeding pool, spread 60-mesh silk screen, place the oxygen tube for inflation in the overhead wooden frame, and spread sand on the spawning area c. Cultivation of the pro-Limulus indoor: the pro-Limulus in step a is placed in step b to carry out indoor aerated culture; d. pro-Limulus lays eggs and ejaculates: when entering the breeding period, the pro-Limulus will lay eggs within 2-10d, Fertilization to form fertilized eggs; e. Screening and washing of fertilized eggs: After the fertilized eggs harden, dig out the fertilized eggs and sand, use a 2mm sieve to wash them, and drain all the sand, leaving fertilized eggs. The method of the present invention is different from the way of killing Limulus crabs to obtain fertilized eggs, and can obtain a large number of high-quality fertilized eggs. The present invention has the advantages of simple operation, high efficiency and easy implementation.

Description

technical field [0001] The invention relates to the technical field of modern rare marine animal breed breeding, in particular to a method for obtaining natural fertilized eggs of horseshoe crabs by adopting overhead sand-laying partitions at the bottom of the pool. Background technique [0002] Horseshoe crabs belong to Limulus, Limulus, and marine arthropods. There are 4 species in total, namely American horseshoe crab, Chinese horseshoe crab, giant horseshoe crab and round-tailed horseshoe crab, which are distributed in Asia and the east coast of North America. Limulus, also known as horseshoe crab, is not a crab, but is related to scorpions, spiders, and extinct trilobites. Limulus is a class of animals as old as trilobites (only fossils now). The ancestor of the horseshoe crab appeared in the Devonian period of the Paleozoic era in the geological history period. At that time, the dinosaurs had not yet risen, and the primitive fish had just come out. As time went by, th...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to change the present situation that the current artificial breeding of horseshoe crabs mainly relies on killing horseshoe crabs and taking eggs and the traditional natural method of egg harvesting is inefficient, so as to create a set of simple and practical methods for obtaining a large number of high-quality fertilized eggs. Lay a solid foundation for hatching, artificial breeding and multiplication and releasing