13 results about "Fertilized Ovums" patented technology

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

The application discloses an artificial breeding and ovum collecting method of horseface fish, which can improve the quantity of obtained eggs, and the difference from the prior art is that the female fish without squeezed eggs is placed in a pool with water temperature of 18-20 DEG C for temporary breeding for 5-7 hours, taken out and placed in water with water temperature of 0-0.5 DEG C for 3-5 minutes, and then the female fish without squeezed eggs is subjected to second artificial egg squeezing and the egg grains are collected, the success rate of the second artificial egg squeezing can reach more than 95%, compared with the prior art of one-time egg squeezing, the quantity of obtained eggs can be improved by more than 20%, that is, the rate of inducing spawning is improved, and thus the quantity of fertilized eggs, the fertilization rate, the hatching rate and the total number of water flowers are improved.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

The utility model discloses a fertilized egg in vitro incubator, including incubator, the incubator is slid and is provided with a plurality of groups of drawers, a plurality of groups drawer all are fixedly connected with the placement ring, the bottom fixed mounting of placement ring has the buffer pad, the drawer is fixedly connected with the limiting block, the both sides wall of incubator all are fixedly connected with the connecting plate, the one side fixed mounting of connecting plate has the sliding block, the outside slide of sliding block is provided with slide rail, the slide rail fixed connection is in the side of drawer, the one end fixed mounting of connecting plate has the damper, in the utility model, through the speed of reducing drawer to the inside movement of incubator, reduce the impact force that the drawer is contacted when closing drawer with incubator box body produces the influence to drawer, thereby reduce the vibration of the petri dish of placing on the drawer, avoid the culture solution in petri dish splash or the fertilized egg shift, guarantee the culture effect of fertilized egg, improve the practicality of incubator.

The present application relates to the technical field of aquaculture, and particularly relates to an artificial breeding method of Cottus hangi in Xingkai Lake. The present application uses LRH-A2 and HCG combination oxytocin to reasonably inject female parent fish and male parent fish, especially to twice injection of female parent fish, and strictly controls injection dose of each injection and reasonable control time interval, which not only can efficiently induce labor, but also can reach 95% of the female parent fish induction efficiency, and can obtain good ovum and semen, so that the ovum can be better fertilized, the quality of the fertilized ovum is improved, the hatching rate is improved, the stress of the parent fish is reduced, and the death of the parent fish is reduced. The present application improves the physical fitness of the parent fish through reasonable parent fish cultivation, and combines reasonable artificial induction operation and post-production nursing measures, so that the post-production death of the parent fish is reduced from multiple aspects, and the post-production survival rate of the parent fish is significantly improved, so that the repeated cultivation cost of the parent fish is reduced.

The present invention relates to a method for in vitro culture of frozen-thaw fertilized eggs using an in vitro culture medium containing 1α,25-Dihydroxyvitamin D3.

The present application belongs to the technical field of aquaculture, and particularly relates to a Decapterus maruadsi breeding feed and application thereof in improving quality of Decapterus maruadsi fertilized eggs. The present application provides a Decapterus maruadsi breeding feed, which comprises two independent first and second feeds; the first feed comprises a basic feed, fish pulp and a nutritional supplement; the second feed comprises the first feed and oyster meat; and the nutritional supplement comprises calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin F. The Decapterus maruadsi breeding feed can be used for nutritional supplement of Decapterus maruadsi parent fish, so as to promote synchronous development of gonads of male and female parent fish, improve quality of Decapterus maruadsi fertilized eggs, and increase good egg rate of Decapterus maruadsi fertilized eggs to 88.50%, which is 39.15% higher than that of a control group, and improve fertilization rate of Decapterus maruadsi fertilized eggs to 79.58%, which is 19.46% higher than that of the control group.

This invention relates to an automatic ice-dropping device and method for large silverfish fertilized eggs. The device includes a diaphragm pump, a fish egg suction tube, a fish egg dropping tube, a floating filter bucket, and a floating traction device. The diaphragm pump is placed on one side of an ice hole in the ice layer. The floating filter bucket is placed in the water of the ice hole. One end of the fish egg suction tube extends into the floating filter bucket, and the other end is connected to the suction port of the diaphragm pump. The floating traction device is placed in the water below the ice layer. One end of the fish egg dropping tube is connected to the outlet of the diaphragm pump, and the other end extends horizontally into the water below the ice layer and is connected to the floating traction device. Several spherical floats are evenly installed on the fish egg dropping tube. By controlling the traction device to make a spiral circular motion around the ice hole, the fish egg dropping tube can be moved synchronously, realizing the uniform dispersal of large silverfish fertilized eggs in the water around the ice hole. The application of this device ensures the uniformity of the egg density, realizes the large-area low-density dispersion of silverfish eggs, improves the quality of hatching and survival rate, expands the release area and scale, and increases the aquaculture yield.

This invention discloses a method for the artificial breeding and selection of small plateau native ornamental fish, belonging to the field of aquaculture technology. The method includes: S1 broodstock domestication, S2 hormone-free spawning induction, S3 fertilized egg hatching, S4 graded initial domestication, and S5 juvenile fish selection. In S2, the broodstock are placed in a spawning induction device, where visual mirror stimulation is generated through a reflecting mirror. Combined with the synergistic effect of gradually increasing light intensity from 8 hours to 12 hours and water temperature from 16°C to 22°C, and the weak electric field generated by the bottom microelectrode array (intensity below the fish's perception threshold, waveform is sine or square wave), natural spawning is induced without the addition of exogenous hormones, achieving a spawning rate of over 87.5%. In S4, the fry are fed graded micro-particle feed in three stages, with particle sizes of 80-120μm, 120-160μm, and 160-200μm, respectively. The fry feeding rate reaches over 87%, and the survival rate at 35 days of age reaches over 78%. This invention enables the artificial breeding of native ornamental fish in the plateau with zero hormones, and is simple to operate with a high survival rate.

The application discloses a constant-temperature compartmentalized procambarus clarkii clutch egg hatching device, and belongs to the technical field of aquaculture. In view of the problems of low hatching rate caused by the mutual interference of parents and the mechanical damage of fertilized eggs in the traditional hatching process, the device comprises a hatching pool with a smooth bottom, a water inlet is arranged on one side of the hatching pool, and an inner plug pipe overflow outlet capable of being pulled up and inserted is arranged at the bottom of the other end of the hatching pool; at least one procambarus clarkii clutch egg compartment module is arranged in the hatching pool, a plurality of cylindrical compartments are arranged in the module, a passageway is arranged between every two rows of compartments, four openings in a cross four-end position are formed in the bottom of each compartment, the compartments, the adjacent compartments and the adjacent passageways are connected with each other through the openings, and the openings allow water to flow but prevent clutch female crayfish from passing; and a constant-temperature system comprising a heating element and a temperature sensor, the heating element is automatically started and stopped according to real-time data of the temperature sensor. The device is mainly used for the industrial hatching of procambarus clarkii fertilized eggs.