132 results about "Fertilized Ovums" patented technology

The utility model discloses a demersal roe hatching device, which relates to the technical field of roe hatching and comprises a hatching container, a hatching sieve bed, a weeping pipe and an overflow pipe, the overflow pipe and the hatching sieve bed are arranged in the hatching container, the top of the hatching container is open, the weeping pipe is arranged above the hatching container and communicated with a water inlet pipe, and a sieve is laid in the hatching sieve bed. Meshes of the screen mesh are smaller than the diameter of the fertilized eggs, so that the fertilized eggs can be flatly laid on the screen mesh before incubation, and the incubation screen bed is at least 10cm lower than an overflow port of the overflow pipe. The fertilized eggs can be flatly laid on the screen in the hatching screen bed in the middle layer of the water body, it is guaranteed that each fertilized egg can obtain sufficient dissolved oxygen supply of the water body, the development requirement is met, hatched fries can fall into the bottom of the hatching container from the screen in time, and the hatching efficiency is improved. Dead eggs and egg shells can be effectively prevented from being infected with water mildew to harm development of other normal fertilized eggs, the work intensity of manually picking up the dead eggs in a traditional hatching mode is reduced, and the hatching rate of the fertilized eggs and the survival rate of juvenile fishes are increased.

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention provides a method for cultivating hybrid fries of Jinhu and tiger. The method comprises the following steps: selecting a breeding environment; breeding and ripening female parents; management and control before oviposition; insemination; hatching fertilized eggs; fry rearing. According to the method, in the female parent breeding and ripening stage, specific biological bait is controlled to be fed in stages, feeding of the parent fish can be promoted, growth can be accelerated, in cooperation with specific compound feed in the management and control stage before oviposition, favorable conditions are created for subsequent oviposition, and the quality and number of ova can be improved. Low-frequency ultrasonic assisted fertilization is introduced in the artificial insemination stage, so that the activity of ovum sperms can be obviously improved, and the fertilization rate is increased; in the fertilized egg hatching stage, intermittent ultrasound is assisted, the hatching rate can be increased, and the seedling yield of the golden-tiger hybrid spots can be increased; after hatching, transferring into a specific culture pond, slowly releasing beneficial bacteria, improving the adaptability of the fry, adsorbing harmful substances, reducing pathogenic bacteria, stabilizing water quality, promoting the growth of the fry, improving the survival rate and reducing the malformation rate.

The invention is applicable to the technical field of rabbit breeding, and provides a method for increasing the body weight of a rabbit based on a gene editing technology, which comprises the following steps: designing an action target sgRNA on a rabbit NR6A1 gene aiming at an NR6A1 p.P192S site; uniformly mixing the synthesized sgRNA and SpRY-CBE, injecting fertilized eggs, transplanting the fertilized eggs into fallopian tubes of receptor female rabbits, and feeding the receptor female rabbits until production; dNA is extracted from produced young rabbit ear tissues, PCR and sequencing are carried out, and if C at the fifth site in NR6A1 gene target sites is mutated into T, it is proved that single base mutation is obtained, and the NR6A1 gene edited rabbit is obtained. According to the invention, the rabbit variety with increased weight is successfully cultivated; a new thought of animal genetic breeding is developed by utilizing a gene editing technology, the method has application values in promoting improvement of genetic characters of rabbits and improving production performance and economic value of the rabbits, and powerful support is provided for high-quality development of the animal breeding industry in China.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The invention relates to an upwelling circulating water culture system for high-density culture of abalone larvae and a use method. Relates to the technical field of abalone recirculating aquaculture. Comprising a circulating water system, a breeding barrel, an electric one-way check valve, an electric adjusting valve and a drain valve, a water outlet of the circulating water system is connected with a water inlet of the breeding barrel through a water inlet pipeline sequentially provided with the electric one-way check valve and the electric adjusting valve, and a water outlet of the breeding barrel is connected with the water inlet end of the circulating water system through a water return pipe. A water outlet branch is arranged between the electric one-way check valve and the breeding cylinder, and the drain valve is arranged on the water outlet branch. Based on the characteristics of abalone larva culture, a three-dimensional culture system with bottom water rising flow is designed, meanwhile, the water flow direction is controlled to rise in a one-way mode through the water pump and the electric one-way check valve, high-density accumulation and adhesion of fertilized eggs in local areas in the pond are reduced, the metamorphosis rate of abalone larvae subjected to abalone larva circulating water culture is greatly increased, and the survival rate of abalone larvae is increased. And high-density accumulation of fertilized eggs in a local space is avoided.

The invention provides a method and a device for efficiently proliferating sepiella maindroni by combining grazing and raising, and belongs to the field of aquatic animal proliferation. An efficient breeding method for sepiella maindroni integrating grazing and breeding comprises the following steps that an appropriate sea area is selected, fertilized eggs are bred in a breeding facility laid in the downstream direction of a normal fish breeding net cage, namely an empty net cage containing an artificial floating reef, the periphery of the empty net cage is provided with an algae-mussel flow blocking ecological rope, and a cover net is arranged above the empty net cage; the newly hatched cuttlefish can stay in the proliferation facility empty net cage for a long time due to the characteristics of loving reefs and drilling the net and strong space awareness, and slowly escape into natural water after gradually adapting to the proliferation environment. According to the proliferation method, cuttlefishes gradually adapt to the natural environment of the sea area, meanwhile, cuttlefish larvae are well protected enough in the fragile incubation initial stage through protection measures such as net cages, and therefore the proliferation survival rate and the maintenance efficiency are improved, and efficient proliferation is achieved.

The invention discloses a factory-like breeding facility and method for crayfish fry, and belongs to the technical field of crayfish breeding. The facility comprises a breeding bed frame and a spray seedling cultivation pond, a plurality of breeding cylinders which operate independently are placed on the breeding bed frame, meshes are arranged in the breeding cylinders in the horizontal direction, the breeding cylinders are divided into upper and lower independent spaces including an incubation area and an egg collection area by the meshes, a plurality of groups of shrimp nests placed on the meshes are arranged in the incubation area, and a plurality of shrimp nest units are arranged in each group of shrimp nests; a seedling outlet pipe is arranged at the bottom of the breeding cylinder; the method comprises the following steps: selecting and putting egg-carrying parent shrimps, artificially incubating fry, managing and controlling the incubation process, and collecting the fry. Through precise regulation and control of multiple parameters such as water temperature, dissolved oxygen, pH and the like, parent health management and automatic spray fry collection system integration, optimization of a crayfish industrialized breeding technology is achieved, the survival rate of the parent, the synchronous incubation rate and the hatching rate of fertilized eggs are improved, the specification and quality of spray fry are guaranteed, and therefore the yield and quality of crayfish industrialized breeding are improved.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention relates to an artificial cross breeding method of Jinlong hybrid spot new germplasm, which belongs to the technical field of aquatic product distant cross breeding, and comprises the following steps: (1) parent breeding; (2) collecting and storing sperms of epinephelus lanceolatus; (3) carrying out reproductive regulation and ovum acquisition on the epinephelus coioides; (4) artificial preparation and incubation of hybrid fertilized eggs; according to the method, the artificial cross breeding of the female epinephelus coioides and the male epinephelus lanceolatus is realized for the first time, and the new germplasm Jinlong hybrid spots of the epinephelus coioides, which grow fast and are high in survival rate, are cultivated. The body weight of the Jinlong hybrid Epinephelus coioides of 3 months old is increased by 46.45% compared with that of pure Epinephelus coioides; and the survival rate of the new germplasm is increased by 62.50% compared with that of the epinephelus coioides. The industrial problems that artificial spawning of large groupers is difficult, the survival rate of fries is low, and fast-growing new germplasm lacks are effectively solved.

The invention discloses a method for breeding lateolabrax japonicus by utilizing an ARTP mutagenesis technology, which comprises the following steps: taking fertilized eggs of lateolabrax japonicus, carrying out ARTP mutagenesis treatment on the fertilized eggs, then hatching to obtain fish fries, and carrying out fry culture to obtain the lateolabrax japonicus. The ARTP mutagenesis treatment conditions are as follows: the radio frequency power is 120 to 220 W; the starting time is 30 to 90 minutes; the treatment time is 5 to 15 minutes. A lateolabrax japonicus fertilized egg is mutated by utilizing an ARTP mutagenesis technology, the mutated lateolabrax japonicus is screened, and the lateolabrax japonicus mutant with excellent characters is screened out.

The utility model discloses a device for fixing fertilized eggs of rainbow trout, which belongs to the technical field of aquaculture and comprises a box body, the top of the box body is fixedly connected with a pair of slide rails, and each slide rail is slidably connected with a support frame. By arranging the sliding rail, the supporting frame, the first bevel gear, the first threaded rod and the transmission assembly, the position of the egg box is adjusted, the supporting frame can slide on the sliding rail and drive the egg box to move horizontally, the transmission assembly drives the first bevel gear to rotate, the first threaded rod can drive the egg box to ascend and descend, it is ensured that the egg box is in a suitable environment, and the hatching rate is increased; through the arrangement of the connecting plate and the bolt assembly, the mode that the egg box is rapidly assembled and disassembled, the sliding blocks are matched with the sliding grooves and the bolt assembly is fixed is achieved, the stability of the egg box after installation is guaranteed, the risk that fertilized eggs are damaged due to shaking is reduced, the egg box can be rapidly disassembled as needed, and the egg box can be conveniently cleaned, overhauled or replaced.

The utility model belongs to the technical field of sea cucumber breeding, and discloses an egg washing and water changing device for sea cucumber breeding, which comprises a net rack assembly for filtering and preventing fertilized eggs from entering, and a siphon assembly which is arranged in the middle of the net rack assembly and is used for supporting water in a siphon pool to be discharged, the siphon assembly is provided with a back-flushing assembly used for flushing fertilized eggs along with the rising and falling of the water level; the siphon assembly comprises a fixing frame, a fixing circular groove is formed in the lower portion of the fixing frame, a siphon is installed in the fixing circular groove, and a hanging frame is arranged on the bottom face of the fixing circular groove. The back-flushing assembly comprises a floating frame, and two limiting rods are symmetrically installed in the floating frame. According to the egg washing and water changing device for sea cucumber seedling culture, fertilized eggs are washed away through back flushing, so that the labor intensity of workers is reduced, and time and labor are saved; through the arrangement of floating and lifting along with the water level, the flushing accuracy is ensured; and through the arrangement of back flushing from inside to outside, fertilized eggs are prevented from being exposed and extruded to be damaged.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

The application discloses an artificial breeding and ovum collecting method of horseface fish, which can improve the quantity of obtained eggs, and the difference from the prior art is that the female fish without squeezed eggs is placed in a pool with water temperature of 18-20 DEG C for temporary breeding for 5-7 hours, taken out and placed in water with water temperature of 0-0.5 DEG C for 3-5 minutes, and then the female fish without squeezed eggs is subjected to second artificial egg squeezing and the egg grains are collected, the success rate of the second artificial egg squeezing can reach more than 95%, compared with the prior art of one-time egg squeezing, the quantity of obtained eggs can be improved by more than 20%, that is, the rate of inducing spawning is improved, and thus the quantity of fertilized eggs, the fertilization rate, the hatching rate and the total number of water flowers are improved.

The invention discloses a Tibetan ink head fish artificial propagation method, and belongs to the field of aquaculture. The Tibetan ink head fish artificial propagation method comprises the following steps that male fish parents and female fish parents which are sexually mature are selected, and spawning induction substances are injected into the female fish twice; the male fish injects spawning induction substances at one time when the female fish is injected for the second time; carrying out in-vitro artificial insemination 12 hours after injecting the oxytocic substance to obtain fertilized eggs; hatching the fertilized eggs, increasing the concentration of lysozyme in a hatching system after the membranes of the fish fries are broken in the hatching process, and artificially assisting the membranes to obtain the Tibetan black head fish fries; and feeding the Tibetan ink head fish fries in stages. According to the artificial propagation method for the ink head fish in Tibetan, the fry of the ink head fish in Tibetan is successfully obtained, the quality and the survival rate of the fry are guaranteed, and technical support is provided for artificial propagation of the fry.

The invention discloses a leiocassis longirostris fry large-scale cultivation method based on a facility circulating water system, and relates to the field of artificial breeding, and the method comprises the steps of indoor parent intensive cultivation, fertilized egg batch acquisition, fry incubation in a loop incubation pond, fry large-scale cultivation and the like. And water purification treatment and efficient oxygenation are realized. Different circulating water operation modes are adopted in the seedling breeding and domestication cultivation process, the optimal breeding ecological condition is provided, and the process is controllable. According to the Leiocassis longirostris fry hatching and breeding method, the Leiocassis longirostris fry hatching and breeding based on circulating water are adopted for Leiocassis longirostris fry large-scale breeding of a facility circulating water system, accurate control over the Leiocassis longirostris hatching process can be achieved, water quality stability is achieved, the water temperature can be accurately controlled, and water changing and wastewater discharging in the whole process are avoided.

The invention discloses a sargassum fusiforme fertilized egg suspension culture new germplasm creation and evaluation method. The method comprises the steps of wild sargassum fusiforme geographical parent selection, isolated culture and unit quality evaluation. Screening male and female plants of parent high-value character strains, performing isolated culture, and preventing and treating harmful organisms; removing epiphytic harmful organisms of male and female plants of the high-value character strain; creating an optimal environmental factor for indoor temporary rearing of lateral branches of male and female plants of the high-value character strain; indoor temporary rearing treatment and temporary rearing time of lateral branches of male and female plants of the parent high-value character strain; classifying and collecting new germplasm fertilized eggs, removing impurities, cleaning, and creating a suspension system of the new germplasm fertilized eggs; preventing and controlling typical harmful organisms of a suspension system in a suspension growth and development stage from new germplasm embryos to juvenile sporophytes; carrying out rope clamping and indoor temporary rearing on new germplasm juvenile sporophytes; stocking the new germplasm juvenile sporophytes in a sea area; an evaluation method of new germplasms of parents and filial generations; a scientific and standard operation and evaluation method can be provided for new sargassum fusiforme germplasm creation, new germplasm quality evaluation and new variety cultivation.

The artificial incubation device comprises a backflow purification tank, an incubation tank and a plurality of incubation containers, the incubation containers are clamped in the incubation tank, each incubation container is in a hollow bullet shape, a filter opening is formed in one side of the top of each incubation container, a screening net is installed on each filter opening, and the other side of each incubation container is provided with a filter screen. The backflow purification tank is arranged on one side of the incubation tank, a water pump is installed at the bottom in the backflow purification tank, an outlet pipe of the water pump is connected with a plurality of branch pipes, and the branch pipes are inserted into the incubation containers and correspond to the incubation containers in a one-to-one mode. The artificial hatching device for the cheilocheilus undulatus is used for hatching and culturing fertilized eggs of the cheilocheilus undulatus into larvae, and the larvae cannot sink to the bottom in the hatching process, so that the larvae cannot die due to oxygen deficit; water flow inside and outside the container is exchanged through the screening net on the incubation container, then the exchanged impurities enter the purification tank from the overflow port and then flow back into the incubation tank through the water pump, a circulation is formed, the water body is kept fresh, and the incubation survival rate of the cheilocheilus undulatus after the cheilocheilus undulatus goes out of the membrane is improved.

The invention discloses shellfish spermiation and ovulation monitoring and fertilized egg collecting and cleaning equipment, and relates to the technical field of shellfish culture. The shellfish spermiation and ovulation monitoring and fertilized egg collecting and cleaning equipment comprises a sparse fishing net, a dense fishing net, a hoisting mooring rope, a sliding assembly, a mechanical arm assembly, a cleaning module and a monitoring module. The dense-net scoop net is arranged below the sparse-net scoop net and is detachably connected with the sparse-net scoop net, and the dense-net scoop net and the sparse-net scoop net are different in mesh aperture (the aperture of the sparse-net scoop net is larger than that of the dense-net scoop net) so as to separate mucus, impurities and The monitoring module is a Hall sensor or a computer vision sensor; the Hall sensor needs to be adsorbed on one side of a shellfish shell to ensure that physiological activities of the shellfish are accurately sensed; a computer vision sensor needs to be matched with a camera (or a shooting module), equipment needs to be installed in an area parallel to shellfishes (most shellfishes are small in head, and rapid opening and closing actions of the shellfishes cannot be clearly observed when the shellfishes are overlooked or looked up), the visual angle of naked eyes can be simulated through parallel installation, and spermiation and ovulation behaviors can be accurately judged.

The utility model provides a multifunctional fish breeding and culturing facility. The multifunctional fish breeding and culturing facility comprises a hatching spraying facility, a movable partition plate, a culturing water inlet pipe, a culturing tank body, a hatching frame and a culturing water outlet pipe, the depth of the cylinder body is gradually increased along the water flow direction; partition plate inserting grooves are formed in the inner wall of the breeding tank body at intervals in the water flow direction, and the movable partition plate is inserted into each partition plate inserting groove; the breeding space of the breeding tank body is divided into a plurality of breeding subareas through the movable partition plates. According to the utility model, intensive culture facilities are adopted to solve the problems of viscous fertilized egg incubation, culture of fishes with different habits and partitioned culture of fishes with different specifications in different stages, so that the production and culture in the whole stage from parent fish culture, parent fish spawning and fertilization, fertilized egg incubation to open fry culture, fry culture and fish culture are realized; the artificial injury to the fishes caused by frequent conversion of culture facilities in the culture process is avoided, and the culture efficiency and effect of the fishes are improved.

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

The invention belongs to the technical field of breeding of aquatic animals, and particularly relates to a breeding method for breeding XX pseudo male fishes by using Chrysophila multistriata. The invention relates to a breeding method for breeding XX pseudo-male fishes by using Christia polyrrhiza, which comprises the following steps of: feeding a feed containing an aromatase inhibitor to Christia polyrrhiza fry with proper day age for induction to obtain pseudo-male fishes with physiological male property and genetic female property; the method comprises the following steps: performing PIT living body electronic marking, then collecting a tail fin tissue sample, performing PCR detection by utilizing a genetic sex specific molecular marker of the Chrysophila polyrrhiza, and screening out pseudo male individuals with female genetic sex and male physiological sex; the obtained pseudo male fish individuals serve as male parents, normal genetic female fishes serve as female parents, intensive cultivation and artificial spawning induction are conducted, and fertilized eggs are obtained; the obtained fertilized eggs are collected and hatched, and pseudo male fishes with all genotypes being XX are obtained. According to the method, the genetic all-female rate of the breeding offspring reaches 100%, and the production purity of the offspring seeds is greatly improved.

This invention discloses a highly efficient method for hatching fish adhesive fertilized eggs, specifically including the following steps: Adhesive eggs and sperm are mixed with feathers to form fertilized eggs; 20-40 times their volume of 0.65%-0.9% physiological saline is added, and the mixture is further mixed with feathers to form a fertilized egg mixture; the egg-collecting part is horizontally placed in the hatching tank using a control mechanism, with the egg-collecting part 10-20 cm above the water surface; the fertilized egg mixture is then poured into a funnel, and the funnel is moved back and forth and side to side to ensure the fertilized egg mixture flows evenly onto the egg-collecting part; the egg-collecting part is rotated using a control mechanism to ensure that all positions on the egg-collecting part are covered with fertilized eggs; and finally, the egg-collecting part with the fertilized eggs attached is vertically hung in the hatching tank using a control mechanism. The egg-collecting part of this invention includes a straight rod and multiple sets of filaments. The multiple sets of filaments are distributed vertically along the straight rod to form a columnar structure. The columnar structure has a three-dimensional spatial shape, good air permeability, and can collect eggs in different areas, resulting in a high egg carrying capacity. Furthermore, it prevents the fertilized eggs from piling up and squeezing each other, effectively avoiding cross-infection.

The invention discloses a perinereis aibuhitensis triploid induction method, which comprises the following steps: placing perinereis aibuhitensis fertilized eggs in ice water for cold shock treatment, then carrying out gradient heating to room temperature, selecting sexually mature perinereis aibuhitensis for artificial insemination, cutting female and male perinereis aibuhitensis into pieces in a stainless steel metal filtering cylindrical net in sequence, and carrying out freeze drying to obtain the perinereis aibuhitensis triploid. According to the method, the stainless steel metal filtering cylindrical net is used for assisting the artificial insemination of the perinereis aibuhitensis, the triploid of the perinereis aibuhitensis is induced through cold shock treatment, the perinereis aibuhitensis induction rate is identified to be 80% or above by means of a flow cytometry and tissue slices, the operation is simple, the cost is low, and the method is suitable for large-scale popularization and application. The cost is saved, and the method is effective and can be used for inducing the marine annelids triploid.

The application provides a big-nose gobi-ovum simulation ecological hatching device, which comprises a hatcher floating on the water surface and a rotating mechanism, the hatcher comprises a hatching net disc and a buoyancy plate, the hatching net disc is freely rotatably connected to one side of the rotating mechanism, a plurality of paddles are annularly and uniformly arranged on the outer side wall of the hatching net disc, the buoyancy plate is annularly arranged on the outer side wall of the hatching net disc and located above the paddles, the hatching net disc is provided with buoyancy by the buoyancy plate, the rotating mechanism drives the hatching net disc to drift around the circumference thereof on the water surface, water flow is exchanged with water in the hatching net disc and is disturbed, the impact force of the water flow is reduced by the hatching net disc, the phenomenon of egg membrane rupture is reduced, and in the drifting process of the hatching net disc, the water flow impacts the paddles, the hatching net disc rotates, the four peripheral side walls of the hatching net disc are uniformly exchanged with the water flow, the big-nose gobi-ovum is dispersed in the hatching net disc, the big-nose gobi-ovum is prevented from gathering together to cause oxygen deficiency, and the hatching rate is improved.

The invention belongs to the technical field of aquaculture, and particularly relates to a decapterus maruadsi breeding feed and application thereof in improving the quality of decapterus maruadsi fertilized eggs. The invention provides a decapterus maruadsi breeding feed. The feed comprises a first feed and a second feed which are mutually independent, the first feed comprises a basal feed, fish cream and a nutrition enhancer; the second feed comprises the first feed and oyster meat; the nutrition enhancer contains calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin F. When the culture feed for the decapterus maruadsi is used for performing nutrient enrichment on parent fishes of the decapterus maruadsi, synchronous development of sexual glands of male and female parent fishes of the decapterus maruadsi can be promoted, the quality of fertilized eggs of the decapterus maruadsi is improved, the good egg rate of the fertilized eggs of the decapterus maruadsi reaches 88.50% and is 39.15% higher than that of a control group, and the fertilization rate of the fertilized eggs reaches 79.58% and is 19.46% higher than that of the control group.

The invention relates to a method for constructing a full-sib family of micropterus salmoides, and belongs to the technical field of aquatic breeding. The method comprises the following steps: constructing a spawning pond system which comprises a spawning pond, a spawning platform, a purse net, spawning nests and spawning palm sheets; selecting male and female parents, matching according to a ratio of 1: 1, and injecting oxytocic hormone; putting the male and female parents into the enclosure net to lay eggs, and collecting fertilized egg palm pieces; carrying out wet transportation by using a fertilized egg transportation device; fertilized eggs are put into a seedling cultivation device to be hatched and screened for cultivation, and the specifications of seedlings are consistent; and finally transferring to a pond net cage for large-scale seedling cultivation. By improving the spawning pond facility, the transportation device and the breeding device, synchronous spawning of the largemouth bass family is achieved, the survival rate and the hatching rate of fertilized eggs are high, the specifications of seedlings are consistent, mutual indigestion is reduced, and the family construction efficiency is improved.