87 results about "Fertilized Ovums" patented technology

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The utility model discloses a device for fixing fertilized eggs of rainbow trout, which belongs to the technical field of aquaculture and comprises a box body, the top of the box body is fixedly connected with a pair of slide rails, and each slide rail is slidably connected with a support frame. By arranging the sliding rail, the supporting frame, the first bevel gear, the first threaded rod and the transmission assembly, the position of the egg box is adjusted, the supporting frame can slide on the sliding rail and drive the egg box to move horizontally, the transmission assembly drives the first bevel gear to rotate, the first threaded rod can drive the egg box to ascend and descend, it is ensured that the egg box is in a suitable environment, and the hatching rate is increased; through the arrangement of the connecting plate and the bolt assembly, the mode that the egg box is rapidly assembled and disassembled, the sliding blocks are matched with the sliding grooves and the bolt assembly is fixed is achieved, the stability of the egg box after installation is guaranteed, the risk that fertilized eggs are damaged due to shaking is reduced, the egg box can be rapidly disassembled as needed, and the egg box can be conveniently cleaned, overhauled or replaced.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

The application discloses an artificial breeding and ovum collecting method of horseface fish, which can improve the quantity of obtained eggs, and the difference from the prior art is that the female fish without squeezed eggs is placed in a pool with water temperature of 18-20 DEG C for temporary breeding for 5-7 hours, taken out and placed in water with water temperature of 0-0.5 DEG C for 3-5 minutes, and then the female fish without squeezed eggs is subjected to second artificial egg squeezing and the egg grains are collected, the success rate of the second artificial egg squeezing can reach more than 95%, compared with the prior art of one-time egg squeezing, the quantity of obtained eggs can be improved by more than 20%, that is, the rate of inducing spawning is improved, and thus the quantity of fertilized eggs, the fertilization rate, the hatching rate and the total number of water flowers are improved.

The invention discloses a leiocassis longirostris fry large-scale cultivation method based on a facility circulating water system, and relates to the field of artificial breeding, and the method comprises the steps of indoor parent intensive cultivation, fertilized egg batch acquisition, fry incubation in a loop incubation pond, fry large-scale cultivation and the like. And water purification treatment and efficient oxygenation are realized. Different circulating water operation modes are adopted in the seedling breeding and domestication cultivation process, the optimal breeding ecological condition is provided, and the process is controllable. According to the Leiocassis longirostris fry hatching and breeding method, the Leiocassis longirostris fry hatching and breeding based on circulating water are adopted for Leiocassis longirostris fry large-scale breeding of a facility circulating water system, accurate control over the Leiocassis longirostris hatching process can be achieved, water quality stability is achieved, the water temperature can be accurately controlled, and water changing and wastewater discharging in the whole process are avoided.

The invention discloses shellfish spermiation and ovulation monitoring and fertilized egg collecting and cleaning equipment, and relates to the technical field of shellfish culture. The shellfish spermiation and ovulation monitoring and fertilized egg collecting and cleaning equipment comprises a sparse fishing net, a dense fishing net, a hoisting mooring rope, a sliding assembly, a mechanical arm assembly, a cleaning module and a monitoring module. The dense-net scoop net is arranged below the sparse-net scoop net and is detachably connected with the sparse-net scoop net, and the dense-net scoop net and the sparse-net scoop net are different in mesh aperture (the aperture of the sparse-net scoop net is larger than that of the dense-net scoop net) so as to separate mucus, impurities and The monitoring module is a Hall sensor or a computer vision sensor; the Hall sensor needs to be adsorbed on one side of a shellfish shell to ensure that physiological activities of the shellfish are accurately sensed; a computer vision sensor needs to be matched with a camera (or a shooting module), equipment needs to be installed in an area parallel to shellfishes (most shellfishes are small in head, and rapid opening and closing actions of the shellfishes cannot be clearly observed when the shellfishes are overlooked or looked up), the visual angle of naked eyes can be simulated through parallel installation, and spermiation and ovulation behaviors can be accurately judged.

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

The invention belongs to the technical field of breeding of aquatic animals, and particularly relates to a breeding method for breeding XX pseudo male fishes by using Chrysophila multistriata. The invention relates to a breeding method for breeding XX pseudo-male fishes by using Christia polyrrhiza, which comprises the following steps of: feeding a feed containing an aromatase inhibitor to Christia polyrrhiza fry with proper day age for induction to obtain pseudo-male fishes with physiological male property and genetic female property; the method comprises the following steps: performing PIT living body electronic marking, then collecting a tail fin tissue sample, performing PCR detection by utilizing a genetic sex specific molecular marker of the Chrysophila polyrrhiza, and screening out pseudo male individuals with female genetic sex and male physiological sex; the obtained pseudo male fish individuals serve as male parents, normal genetic female fishes serve as female parents, intensive cultivation and artificial spawning induction are conducted, and fertilized eggs are obtained; the obtained fertilized eggs are collected and hatched, and pseudo male fishes with all genotypes being XX are obtained. According to the method, the genetic all-female rate of the breeding offspring reaches 100%, and the production purity of the offspring seeds is greatly improved.

This invention discloses a highly efficient method for hatching fish adhesive fertilized eggs, specifically including the following steps: Adhesive eggs and sperm are mixed with feathers to form fertilized eggs; 20-40 times their volume of 0.65%-0.9% physiological saline is added, and the mixture is further mixed with feathers to form a fertilized egg mixture; the egg-collecting part is horizontally placed in the hatching tank using a control mechanism, with the egg-collecting part 10-20 cm above the water surface; the fertilized egg mixture is then poured into a funnel, and the funnel is moved back and forth and side to side to ensure the fertilized egg mixture flows evenly onto the egg-collecting part; the egg-collecting part is rotated using a control mechanism to ensure that all positions on the egg-collecting part are covered with fertilized eggs; and finally, the egg-collecting part with the fertilized eggs attached is vertically hung in the hatching tank using a control mechanism. The egg-collecting part of this invention includes a straight rod and multiple sets of filaments. The multiple sets of filaments are distributed vertically along the straight rod to form a columnar structure. The columnar structure has a three-dimensional spatial shape, good air permeability, and can collect eggs in different areas, resulting in a high egg carrying capacity. Furthermore, it prevents the fertilized eggs from piling up and squeezing each other, effectively avoiding cross-infection.

The invention discloses a perinereis aibuhitensis triploid induction method, which comprises the following steps: placing perinereis aibuhitensis fertilized eggs in ice water for cold shock treatment, then carrying out gradient heating to room temperature, selecting sexually mature perinereis aibuhitensis for artificial insemination, cutting female and male perinereis aibuhitensis into pieces in a stainless steel metal filtering cylindrical net in sequence, and carrying out freeze drying to obtain the perinereis aibuhitensis triploid. According to the method, the stainless steel metal filtering cylindrical net is used for assisting the artificial insemination of the perinereis aibuhitensis, the triploid of the perinereis aibuhitensis is induced through cold shock treatment, the perinereis aibuhitensis induction rate is identified to be 80% or above by means of a flow cytometry and tissue slices, the operation is simple, the cost is low, and the method is suitable for large-scale popularization and application. The cost is saved, and the method is effective and can be used for inducing the marine annelids triploid.

The application provides a big-nose gobi-ovum simulation ecological hatching device, which comprises a hatcher floating on the water surface and a rotating mechanism, the hatcher comprises a hatching net disc and a buoyancy plate, the hatching net disc is freely rotatably connected to one side of the rotating mechanism, a plurality of paddles are annularly and uniformly arranged on the outer side wall of the hatching net disc, the buoyancy plate is annularly arranged on the outer side wall of the hatching net disc and located above the paddles, the hatching net disc is provided with buoyancy by the buoyancy plate, the rotating mechanism drives the hatching net disc to drift around the circumference thereof on the water surface, water flow is exchanged with water in the hatching net disc and is disturbed, the impact force of the water flow is reduced by the hatching net disc, the phenomenon of egg membrane rupture is reduced, and in the drifting process of the hatching net disc, the water flow impacts the paddles, the hatching net disc rotates, the four peripheral side walls of the hatching net disc are uniformly exchanged with the water flow, the big-nose gobi-ovum is dispersed in the hatching net disc, the big-nose gobi-ovum is prevented from gathering together to cause oxygen deficiency, and the hatching rate is improved.

The invention belongs to the technical field of aquaculture, and particularly relates to a decapterus maruadsi breeding feed and application thereof in improving the quality of decapterus maruadsi fertilized eggs. The invention provides a decapterus maruadsi breeding feed. The feed comprises a first feed and a second feed which are mutually independent, the first feed comprises a basal feed, fish cream and a nutrition enhancer; the second feed comprises the first feed and oyster meat; the nutrition enhancer contains calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin F. When the culture feed for the decapterus maruadsi is used for performing nutrient enrichment on parent fishes of the decapterus maruadsi, synchronous development of sexual glands of male and female parent fishes of the decapterus maruadsi can be promoted, the quality of fertilized eggs of the decapterus maruadsi is improved, the good egg rate of the fertilized eggs of the decapterus maruadsi reaches 88.50% and is 39.15% higher than that of a control group, and the fertilization rate of the fertilized eggs reaches 79.58% and is 19.46% higher than that of the control group.

The invention relates to a method for constructing a full-sib family of micropterus salmoides, and belongs to the technical field of aquatic breeding. The method comprises the following steps: constructing a spawning pond system which comprises a spawning pond, a spawning platform, a purse net, spawning nests and spawning palm sheets; selecting male and female parents, matching according to a ratio of 1: 1, and injecting oxytocic hormone; putting the male and female parents into the enclosure net to lay eggs, and collecting fertilized egg palm pieces; carrying out wet transportation by using a fertilized egg transportation device; fertilized eggs are put into a seedling cultivation device to be hatched and screened for cultivation, and the specifications of seedlings are consistent; and finally transferring to a pond net cage for large-scale seedling cultivation. By improving the spawning pond facility, the transportation device and the breeding device, synchronous spawning of the largemouth bass family is achieved, the survival rate and the hatching rate of fertilized eggs are high, the specifications of seedlings are consistent, mutual indigestion is reduced, and the family construction efficiency is improved.

The invention provides a method for efficiently inducing larimichthys crocea triploid by using hydrostatic pressure, which comprises the following steps of: immediately transferring fertilized eggs into a hydrostatic pressure device 2 minutes and 30 seconds after artificial insemination of larimichthys crocea sperm eggs, applying pressure of 7000-8000 PSI, and performing hydrostatic pressure treatment for 3 minutes. Wherein in the hydrostatic pressure treatment process, it should be ensured that the pressure is stable, and the fluctuation range does not exceed + / -1%. According to the method, the time and pressure of hydrostatic pressure treatment are accurately controlled, the triploid induction rate can be 90% or above, and the induction rate is high. The damage to fertilized eggs is reduced through optimized processing parameters, the hatching rate of the fries can reach 70% or above, and the hatching rate is high. In addition, the hydrostatic pressure treatment time is only 3 minutes, the operation is efficient, the method is more suitable for large-scale production compared with other methods, and the triploid cultivation efficiency is improved.

The present application relates to the field of aquaculture, and particularly relates to a method for hatching and cultivating fry of Takifugu obscurus, which comprises nine steps of concentrated hatching of fertilized eggs, separation of fertilized eggs, egg spreading, air control, water temperature control, water quality control, bait and feeding method, pool division, and desalination of fry. The present application creates a simple method for hatching and cultivating fry of Takifugu obscurus from fertilized eggs, and particularly changes the early larva carrying into the fertilized egg carrying in the technical scheme, breaks through the existing cognition, and creates a simple operation of moving the Takifugu obscurus from the conical hatching barrel into the flat bottomed fry cultivation pool before the membrane of the fertilized egg is broken, and a late embryo membrane breaking and fry cultivation technical scheme. The present application improves the Takifugu obscurus breeding efficiency and reduces the artificial breeding cost. The present application saves the breeding production cost, is simple to operate, and is easy to be accepted and popularized by people.

The invention discloses an artificial maturity promoting and fry breeding method for Peltella corii, which comprises the steps of cooling temporary rearing, maturity promoting, mating, spawning, hatching, high-density larva breeding, metamorphosis and the like, male snails and female snails are separately bred during maturity promoting, and a breeding scheme is optimized. According to the technical scheme, batch gonad maturation of parent snails according to plans is achieved, and the maturity is good; high-density breeding technologies such as fertilized egg hatching and larva breeding are overcome, a stable and efficient fry production process is established, a large number of high-quality, healthy and regular-specification young snails are provided for the breeding industry, and the provenance basis of the industry is fundamentally guaranteed.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

The present application belongs to the technical field of fish genetic breeding, and discloses a method for improving salt tolerance of grass carp, and specifically discloses a method for improving salt tolerance of fish, which comprises the following steps: taking fish fertilized eggs as initial domestication objects, and placing the fish fertilized eggs in salt water for hatching and domestication; thereafter, the salinity of the water body is increased by 1‰-2‰ every 25-35 days until the salinity is the same as that of the target aquaculture water body. The present application provides a footprint method based on salinity gradual domestication to improve the salt tolerance of fish. The method can effectively activate the expression of tissue osmotic pressure regulation related genes such as gills and kidneys, and ultimately can enable grass carp to adapt to 7‰ water body salinity, breaking the conventional salt tolerance threshold of grass carp. The main performance is that the grass carp still has the characteristics of rapid growth, healthy body, and strong feeding under 7‰ water body salinity.

This invention discloses a device and method for de-adheding fertilized eggs of the spiny loach. The device includes an incubator with a first guide rail and a second guide rail above it. One end of the second guide rail is connected to the first guide rail via a fixing rod. A first frame is movably disposed between the first guide rails, and a temporary storage box is rotatably mounted on the first frame. A first vertical block is connected to the outer wall of the temporary storage box. A second frame is movably disposed between the second guide rails, and a second vertical block is mounted on the second frame. A first linkage mechanism is provided between the second vertical block and the first vertical block to lock or unlock the connection between the second and first vertical blocks. A second linkage mechanism is also provided between the second frame and the fixing rod to lock or unlock the connection between the second frame and the fixing rod. This device for de-adheding fertilized eggs can regulate the de-adhesion and pouring of fertilized eggs, and evenly distribute the fertilized eggs into the incubator.

The device is placed in a dark environment and comprises a hatching barrel, a collector, a water tank, a water pump, a water pipe and an energy-saving lamp, the lower portion of a barrel body of the hatching barrel is in a funnel shape, the upper portion of the barrel body of the hatching barrel is in a tubular shape, and two water pipe connectors are arranged on the hatching barrel and are called an upper water opening and a lower water opening respectively. The upper water port is located at the lowest end of a barrel body of the hatching barrel, the lower water port is located on the upper portion of the barrel body of the hatching barrel, shading stickers are attached to the outer portion of the hatching barrel except the lower water port and the area above the lower water port, the collector is tubular and transversely placed in the water tank, the collector is provided with two water inlets and two water outlets, the water inlets are located in the side wall of the upper portion of the collector, and the water outlets are located in the side wall of the lower portion of the collector. And the opening of the water inlet is upward. According to the device, automatic directional collection can be achieved by combining the phototaxis of the newly hatched larvae of shrimps and crabs with water flow, the step of manually collecting the newly hatched larvae is omitted, and meanwhile the device has mobility and configurability and has good universality and expansibility.

The application discloses a method for forming fertilized eggs of Pseudobrama simus, and relates to the technical field of aquaculture, and comprises the following steps: step one, placing the fertilized eggs into a spray pipe; step two, evenly smearing the fertilized eggs by the water vapor sprayed by an adjusting mechanism and the stirring of a feather rod; step three, controlling the driving state of a driving mechanism by the adjusting mechanism to drive a piston mechanism to spray a large amount of water to flush the fertilized eggs into a hatching cylinder; and step four, adjusting the intensity by a fluctuation mechanism to make the fertilized eggs in a real water body fluctuation environment; the lower piston cylinder and the upper cavity of the double-cavity piston cylinder are controlled to perform piston movement to spray a large amount of gas and a small amount of water, then the water vapor is sprayed to the fertilized eggs in the form of water vapor through the spray pipe, and the blown fertilized eggs are uniformly arranged under the fluctuation of the feather rod; the application can control the fluctuation amplitude and make the fertilized eggs uniformly distributed on the supporting plate, so that the application can avoid the damage of eggshells or the injury of eggs caused by excessive pressure or excessive friction.

The application discloses a kind of Sargassum fusiforme fertilized egg suspension culture new germplasm creation and evaluation method, including wild Sargassum fusiforme geographical parent selection, isolation breeding and unit mass evaluation;Parent high-value character strain female and male plant screening, isolation breeding and enemy biological control;High-value character strain female and male plant epiphytic enemy biological removal;High-value character strain female and male plant lateral branch indoor temporary nursing optimum environmental factor creation;Parent high-value character strain female and male plant lateral branch indoor temporary nursing processing and temporary nursing time;New germplasm fertilized egg classification collection, impurity removal and cleaning, new germplasm fertilized egg suspension system creation;New germplasm embryo to young sporophyte suspension growth and development stage suspension system typical enemy biological control;New germplasm young sporophyte is clamped and indoor temporary nursing;New germplasm young sporophyte is put in sea area;Parent and offspring new germplasm evaluation method;Can be Sargassum fusiforme new germplasm creation, new germplasm quality evaluation and new variety cultivation provide scientific, standard operation and evaluation method.

The application provides a water circulation purification device for a fish sinking fertilized egg transport water cart, which comprises a purification tank and a plurality of transport barrels located on both sides of the purification tank, an inner part of the purification tank is provided with a purification area and two aeration areas, the two aeration areas are respectively communicated with both sides of the purification area, an aeration assembly is arranged in the aeration area, a honeycomb inclined pipe is arranged on the upper part of the purification area, water inlet ends of the plurality of transport barrels are communicated with upper pipes of the purification area, and water outlet ends of the plurality of transport barrels are communicated with upper pipes of the aeration area through a circulating pump. The application can realize water purification and water circulation during long-distance transport of fertilized eggs, improve water resource utilization, maintain water quality, effectively avoid death of the fertilized eggs during transport, and simultaneously avoid generation of a large amount of solid waste.

The mandarin fish egg collecting device comprises an egg laying box horizontally arranged on the ground, and a water inlet is formed in one side of the egg laying box; two flotation devices are symmetrically arranged in the oviposition box, and an adjustable flow guide device is fixed below each flotation device; a separating inclined plate is horizontally arranged in the middle of the interior of the spawning box, a collecting device is fixed to the upper portion of the separating inclined plate, a collecting groove is fixed to one side of the collecting device, and a water outlet is formed in the lower portion of the end of the collecting groove; the two same flotation devices and the adjustable flow guide device are adopted, low-damage stripping and precise flotation separation are achieved, and the problems that in a traditional collection mode, the roe damage rate is high, and impurity separation is not thorough are solved; a flotation device and a separation inclined plate are further adopted, and automatic separation of healthy roes, excrement and unfertilized roes is achieved by means of buoyancy difference.

The invention discloses an automatic collection and separation device for fertilized eggs of lissocheilus undulatus, and relates to the technical field of roe breeding and breeding. A collection cover is mounted at the top of a layering cylinder, a sealing bottom plate is connected to the bottom of the collection cover, one side of the collection cover is connected to one end of a tangential pipe, and a rotational flow pipe is rotationally mounted at the top of the layering cylinder; the top of the rotational flow pipe penetrates through the sealing bottom plate and is rotationally connected with the sealing bottom plate, a plurality of guide vanes are welded to the inner side of the rotational flow pipe, a blue light lamp ring is installed at the top of the collecting cover, and a conical pipe is connected to the position, located at the bottom of the rotational flow pipe, in the layering barrel. The fertilized eggs are collected and then subjected to layered screening, the fertilized eggs subjected to layered screening can be transferred out of the breeding pool from the breeding pool in the transfer egg storage assembly, the fertilized eggs are conveyed through water flow in the whole process, the fertilized eggs are fully protected, and compared with the prior art, the collection speed of the fertilized eggs is higher, and the quality of the fertilized eggs is higher.

The application discloses a method for inducing tetraploid of Haliotis discus hannai Ino, and relates to a method for cultivating tetraploid of shellfishes, which comprises the following steps: 1) selecting and accelerating maturation of seed abalones: selecting healthy and active male and female seed abalones without external damage, and placing the seed abalones in a seed abalone cultivation pool to accelerate maturation simultaneously; 2) artificially inducing spawning: selecting diploid abalones with well-developed gonads to induce spawning, and stopping until the male and female seed abalones spawn and ejaculate; 3) selecting and mixedly fertilizing sperm and eggs: filtering impurities in sperm and eggs respectively, and synchronously mixedly fertilizing the sperm and the eggs by adding the sperm into the egg liquid; 4) cold shock induction: when 60% to 80% of the fertilized eggs are ready to start the first cleavage and elongation, the fertilized eggs are immediately collected into pre-cooled seawater with a temperature of 2-8 DEG C to be treated for 10-15 min, so as to inhibit the first cleavage; and 5) incubation: immediately placing the fertilized eggs into an incubation water tank with a temperature of 18-22 DEG C to be incubated after the cold shock is finished. The method for inducing tetraploid of Haliotis discus hannai Ino realizes efficient and stable obtaining of tetraploid of Haliotis discus hannai Ino by improving the method for inducing spawning of seed abalones and optimizing the inducing conditions.