387 results about "Fertilized Ovums" patented technology

The invention relates to a method of industrial and artificial breeding of Epinephelus, which is applicable to the breeding of Epinephelus malabaricus and E.coioides. The method comprises the following steps: (1) treating a nursery pond and water; (2) preparing before breeding; (3) putting larval fishes of Epinephelus or germ cells; (4) controlling the quality of the breeding water body; and (5) feeding baits. The method is applied to conduct the industrial and artificial breeding, so that the water quality of the breeding is stable, the output for the unit water body breeding is high, the bred sizes of advanced fries are uniform, no antibacterial drugs are used, no special breeding is conducted to unicellular alga, no bottom-attaching happens in the process of breeding, and the operation is simple.

The invention discloses a method for establishing an immunodeficiency mouse model. The method comprises the following steps of: (1) constructing DNA (deoxyribonucleic acid) which enables functions of an immune related gene in a mouse cell to be lost; (2) purifying mRNA (messenger ribonucleic acid), and cryopreserving in a super-purified water of RNAase free; (4) feeding a pregnant female mouse and a pseudopregnant female mouse under an SPF (specific pathogen free) level feeding condition; (5) taking a fallopian tube from the abdominal cavity of the pregnant female mouse in the step (4), and collecting fertilized ovum; (6) injecting the mRNA obtained in the step (3) into the pronucleus of the fertilized ovum in the step (5) by using a microinjection method; (7) transplanting the fertilized ovum obtained in the step (6) into the body of the pseudopregnant female mouse obtained in the step (4), and enabling the pseudopregnant female mouse to culture the second generation; (8) establishing a stable deficient mouse strain. The method has the advantages that a mouse model which completely does not have immunological rejection to allotransplantation can be obtained for testing.

The invention discloses a manufacturing method of Chinese caterpillar fungus, which is characterized by the following: establishing greenhouse booth moth culturing field according to culturing temperature and moisture; reducing the danger of natural enemy of moth to accelerate weak plant root of herb and bush; releasing certain quantity of moth fertilized ovum, larva, pupal or and imagine in the culturing field; elevating moth larva breeding density with 110-3500000 per hectareha; breeding the moth from one year to 6-8 years within 365-425d; forming sac fungus; collecting 530000-1570000 Chinese caterpillar fungus per hectareha.

The injector of the present invention (10) comprises a heat-retaining tube (12), an outer pipe (18) for inserting into a uterus which is fixed parallel to a front end of the heat-retaining tube (12), a syringe (22) for injection inserted into the heat-retaining tube (12) from the rear end of the heat-retaining tube (12), an elastic and flexible tube for injection (24) which is connected to the front end of the syringe (22) and is pulled out of the front end of the heat-retaining tube and is inserted slidably into inside of the hollow of the outer pipe (18),and a discharge nozzle (28) which is mounted integrally to the front end of the tube (24) and projects from the front end of the tube (18), and in which a portion between a projecting position from the heat-retaining tube (12) and the outer pipe (18) is regarded as a surplus length portion (24a) and the surplus length portion (24a) is paid out in a state that the outer pipe (18) is inserted to a given depth in a uterus, thereby making the discharge nozzle (28) reach to the deep portion of the uterus along the shape of the uterus.

The invention discloses a method for the three-dimensional and artificial seedlings cultivation of the perinereis aibuhitensis, which mainly comprises the steps of: (1) fishing and disinfecting the heteronereis; (2) laying eggs, hatching and cultivating the planktonic larva; (3) three-dimensionally cultivating the benthic larvae; and (4) seedling emerging. The key measures comprise the steps of: putting four setiger intermediate-later stage nectochaete into a polyethylene water tank for the three-dimensional cultivation, cultivating by flowing water, and properly mixing the single-cell algae, the EM (effective microorganisms) bacterium with various artificial baits. After the measures are used, the density of the larvae is reduced, the mutual massacre among the individuals is reduced, the nutritional requirement of each-stage larva and the good and stable water quality environment are guaranteed, the survival rate of the larva is greatly improved, the breeding rate from the fertilized ovum to the sand silkworm seedling with the length of 1cm reaches at 42.3%, and the seedling yield per unit area is improved at 11.4*104 numbers/m<2>, so that the survival rate, the breeding rate and the seedling yield per unit area are greatly improved compared with the existing seedlings cultivation mode. The method is convenient to operate, can prevent the seedlings from being damaged caused by sand flushing and seedling driving, and is good for the long-distance transporting and the planting of the seedlings.

A method for the oviposition and incubation of poulpe includes such steps as temporary culture of parents, repening the sex gland of parents, arranging ovum collectors which are the PVC tube with 20-30 cm in length, oxytocia, moving the female poulpes along with said ovum collectors in a netted cage, aerating while incubating, collecting larvae, and culturing in pool. Its netted cage for incubation is also disclosed.

The invention provides an artificial sea bass breeding method suitable for river mouth areas. According to the method, the artificial sea bass breeding suitable for the river mouth areas is realized by virtue of the steps of parent sea bass selection and domestication, parent fish reproduction, fertilized ovum incubation, indoor fry cultivation, outdoor fry cultivation, fry screening and fry taking-out. The method is used for solving the key problems such as parent sea bass selection standards and domestication environment condition requirements, artificial parent fish ripening and spawning induction methods, artificial fertilization method, fertilized ovum incubation condition requirements, fry cultivation condition requirements, indoor and outdoor fry cultivation and bait transformation; the survival rate of sea bass fry stocking and the breeding efficiency can be remarkably improved; in short, the method is significant for the healthy development of the sea bass breeding industry in the river mouth areas of Pearl River Delta.

The invention discloses a shrimp seed breeding and rearing method for cherax quadricarinatus. The method comprises steps as follows: (1), preparation of a breeding pool; (2), selection of parent shrimps; (3), culture of the parent shrimps; (4), pairing of the parent shrimps; (5), culture of fertilized eggs; (6), hatching management; and (7), nursing of the parent shrimps. The a shrimp seed breeding and rearing method for the cherax quadricarinatus is provided by construction of a culture pool, a mating pool, a hatching pool and a nursing pool, so that in the seed breeding stage, the egg mass rate of the cherax quadricarinatus reaches 70% or higher, the survival rate of the shrimp seeds reaches 80% or higher, the shrimp seed yield is 200% or higher of that in the prior art, and the method has remarkable economic benefits.

The invention provides a method for trapping and controlling Toxoptera aurantii Boyer, which comprises the steps of: with cis-3-hexen-1-ol, trans-2-hexenal, gaultherolin and phenylcarbinol as taste source matters, preparing into a Toxoptera aurantii Boyer botanical pheromone trapping agent according to the proportion of 1:(0.1-15): (0.1-15): (0.1-15), or preparing the gaultherolin, schizonol and nepetalactone into a Toxoptera aurantii Boyer pheromone trapping agent according to the proportion of 1: (0.1-15): (0.1-15), selecting color bud green, rape flower yellow and jasmine yellow to be prepared into a sticky colored plate, combining with a sticky colored plate trapper to prepare a trapper, and placing the trapper into a tea garden for trapping Toxoptera aurantii Boyer or sexuales to reduce overwintering fertilized ova so that the population density in next year is reduced. The method provided by the invention is free of pollution, adapts to the production of current organic tea and non-polluted tea, and has the advantages of high control efficiency, stable performance, reduction of use frequency and use dosage of chemical pesticides, convenience for transportation of raw materials and use, and low cost.

The cultivation method of sea urchin tetraploid fingerlings has the steps that the urchin seedling is dried in shade for 0 to 40 minutes. The 0.5 to 2.0 ml 0.5 mol / L KCl solution is injected from the sea urchin membrana peristomialis for the spawing and spermiation. The urchine eggs and sperms are collected, and the hybrid fertilization is implemented. The developmental temperature of fertilized eggs is controlled between 17 to 18 DEG C. The fertilized eggs in concentration with a mix silk net are collected after 55 to 65 minutes of the fertilization, which are packed in a closed bag and disposed in a pressure vessel of a hydrostatic pressure equipment and the ambient temperature is identified between 17 to 18 DEG C. The pressure treatment is implemented after 60 to 70 minutes of the fertilization, in which the pressure is controlled between 70 to 80MPa and the processing time is controlled between 6 to 8 minutes. Afterwards the fertilized eggs are disposed inside a bin for hatching and stirred, and the incubation temperature is controlled between 16 to 18 DEG C. After floated, the embryos are selected with the proper aeration. The bait feeding is implemented during the larvae cultivation period. The water is exchanged 1 to 3 times everyday, and each time the quantity of water exchange is 1/3 to 2/3. The pond water is exchanged once every three days. The tetraploid fingerlings cultivated with the method can produce the triploid fingerlings, thus the time of processing and the time to the market are extended.

The invention discloses a method for temporary culture, egg laying and egg protecting of octopus variabilis sasaki and a device for the method, wherein the method comprises steps of 1 selection of parents, 2 temporary culture and accelerated ripening of parents and 3 egg protecting of the parents, and an octopus nest which is specifically designed is used. The method for temporary culture, egg laying and egg protecting of the octopus variabilis sasaki is simple in operation, the device for the method is low in cost and high in use rate, can effectively increase survival rate and egg laying amount of the parents of the octopus variabilis sasaki, hatching rate of fertilized eggs and healthy degree of young seedling. The octopus nest simulates a natural nest structure of the octopus variabilis sasaki, is more suitable for actions of patient octopus variabilis sasaki such as hiding, defensing and laying eggs and protecting eggs and the like, and is simple in making, convenient to use, less in investment and excellent in effects.

The invention belongs to field of fish breeding and aquaculture, which in detail relates to a method of artificial crossing with summer-bothus and tooth-Bothus and breeding. It comprises following steps: (1) taking summer-bothus as male parent and tooth-bothus as female parent, reinforcing the nutrient and environmental condition to promote simultaneous gonadal maturity, getting mature sperm and ovum; (2) crossing fertilized ovum and hatching; (3) culturing the hybrid body and young fish; (4) providing systematic bait in culturing period. The invention not only provides material for deseription improvement for bothus and soles, but also the lies foundation of adding a new breeding species for sea fish cultivating.

The invention discloses a method for carrying out incubation on neolissochilus benasi through artificial fertilization, which belongs to the technical field of fish product breeding. The method comprises the following steps of: injecting DOM (domperidone maleate) and LRH-A2 (lutein releasing hormone A2) into gonadosomatic neolissochilus benasi (1mg of DOM and 1 mu g of LRH per kilogram of neolissochilus benasi are injected in per kilogram of gonadosomatic neolissochilus benasi), wherein the injection volume is halved for male neolissochilus benasi, and the effect time is 15 hours at a water temperature of 22 DEG C; extruding ovums and sperms of the gonadosomatic neolissochilus benasi into a container, stirring the ovums and the sperms for 30 seconds by using chicken feather, then adding clear water at a temperature of 22 DEG C into the container (water preferably just submerges the ovums and the sperms), after stirring the obtained mixture for 20 seconds by using the chicken feather, carrying out washing three times by using clear water, then taking the obtained fertilized ovums for backup incubation; putting the fertilized ovums into an aqueous captan solution (0.15 ppm) to soak for 15 minutes, then putting the fertilized ovums into a water-filled dissecting tray and a shallow-water basin (in which the water level is preferably 3-5 cm higher than the ovums) for incubation, wherein 3000-5000 ovums with an ovum diameter of 2.2-2.8 mm are put in each basin, the position of a water-filled container is 50-80 cm higher than the dissecting tray and the shallow-water basin; sucking water by using a small-sized oxygen hose and conveying the water to the dissecting tray and the shallow-water basin to carry out incubation for 120 hours so as to get fish fries, wherein the temperature of the water is 22-23 DEG C, and the pH value of the water is 7.8-8.5. The method disclosed by the invention has the advantages of low cost, easiness in operation, and high incubation rate.

The invention belongs to the field of aquaculture, in particular to a large-scale artificial hatching method of fertilized ovums of rice-field eels. The large-scale artificial hatching method is characterized in that the artificial hatchability can be improved to above 90%, and a technical system can be provided for an imitative ecological large-scale breeding by taking neural embryos as artificial hatching starting points, applying special hatching facilities, and continuous dripping for oxygenation and other methods. The large-scale artificial hatching method comprises the following steps: 1) building special hatching facilities, and installing a water storage tank, a hatching tank and a dripping facility sequentially according to a high position difference; 2) adopting an imitative ecological breeding method to obtain the fertilized ovums of the ricefield eels, transferring the fertilized ovums to a hatching box to hatch when the fertilized ovums naturally develop to a neural embryonic period in a foam nest; 3) paving the neural embryos in the hatching box, keeping the embryos 1cm far away from the water surface, and promoting the neural embryos to develop by adopting a dripping method for oxygenation; and 4) continuously leaving baby eels to cultivate in the hatching box temporarily after the baby eels come out of membranes, feeding until the eels is 5cm long through opening and tamping and then transferring the eels to a net cage or other facilities to continuously feed.

The invention discloses a cross breeding method for pelteobagrus fulvidraco and pseudobagrus vachelli. The wild-caught or market pelteobagrus fulvidraco and the wild-caught or market pseudobagrus vachelli are temporarily reared and domesticated to reach the parent fish standard, after artificial injection induced spawning and fertilization, fertilized ova are evenly attached to an incubation net, the fertilized ova are incubated in a cellular incubation pool through micro-flow water, and then crossbred fry are obtained. The pelteobagrus fulvidraco and pseudobagrus vachelli artificially-crossbred fry which are quick in growth, high in stress resistance and better in meat quality can be obtained, and the cross breeding method has significance in meeting the living needs of people and increasing freshwater aquaculture new varieties.

The invention discloses a method for breeding a sipunculus nudus parent. Artificial breeding of sipunculus nudus is high in cost, very instable in effect and significantly relevant to poor artificial spawning effect, high mortality and inconsistent egg laying quality of the sipunculus nudus parent. Meanwhile, mismatch of the yield is caused due to excessive concentration of egg laying in induced spawning, leading to resource waste. By adopting the measures of building of a sipunculus nudus parent breeding pond, indoor artificial food culture, maturing and breeding management, disease control and prevention and artificial control of egg laying, the mortality is lowered to 7-12 percent within 40 days of artificial indoor parent maturing and egg laying breeding, the single yield of an individual parent of 20g is 50,000-70,000 eggs, the metamosphsis rate of hatched larvae is 13-86 percent, and artificial control of egg laying can be performed according to the production capacity. By adopting the method, the source and controllability of fertilized eggs are realized in plant artificial breeding and selective breeding of the sipunculus nudus, the egg laying time of the parent is prolonged, and the breeding cost is saved.

The invention discloses a seed production and culture method of a carp with short growth period and comprises the steps of the selection of breeding parent, which determines Jinxin female carp and Ukrainian scaled male carp as seed production parents through the RAPD technology, grouping of the male and female parents with a proportion of 1 to 1.2-1.5, reproduction of fish seeds by the methods of artificial flushing, natural spawning or artificial insemination, incubation in the step of which artificial fish egg collectors with naturally produced eggs and artificial fish egg collectors with fertilized ovum through artificial dousing are delivered to a pond for natural incubation or the fertilized ovum through artificial insemination is doused in the waxed pond bottom and then placed into a ring channel for incubation after being collected and washed, and initial culture and commercial fish culture: each Mu of pond can culture 2,000-2,200 pieces of summer fingerlings or 1200-1400 pieces of spring fingerlings and is matched with silver carp fingerlings or spring fingerlings which account for 20 percent of the main culture objects. The fish can be cultured in the current year. The carp with short growth period cultured by the method of the invention has the advantages of unique shape, rapid growth, strong disease resistance and high yield.

The invention discloses a method for artificial reproduction of brachymystax lenok tsinlingensis. The method for artificial reproduction of the brachymystax lenok tsinlingensis comprises the steps that (A) mature and highly active brachymystax lenok tsinlingensis is selected to serve as a parent; (B) reinforced cultivation of the parent is conducted; (C) artificial spawning is conducted, wherein two dosages of artificial spawning medicine is injected into the parent for artificial spawning from the end of March to the middle of April; (D) semen collection is conducted manually, wherein the parent is bred in an artificial spawning pond after the artificial spawning medicine is injected into the parent, a water inlet pipe is erected at one end of the artificial spawning pond, and the abdomen of the brachymystax lenok tsinlingensis parent is pressed regularly so that semen collection can be achieved from the alive brachymystax lenok tsinlingensis parent; (E) artificial insemination is conducted, wherein after an ovum is rinsed with normal saline for fish is collected, seminal fluid of a milter is squeezed on the ovum, then mixing is conducted with a feather, andartificial insemination is conducted; (F) artificial incubation is conducted, wherein a fertilized ovum is washed with purified water and then is poured in an incubation groove for incubation, a porous dandruff comb is arranged on the bottom of the incubation groove, and when fry which can swim appears, the fry is moved out of an incubation device to be bred in a fish tank. The method for artificial reproduction of the brachymystax lenok tsinlingensis is easy to implement, operation is easy and convenient, the fertilization rate and the hatching rate are high, obtained fish offspring are healthy, the body sizes of the obtained fish offspring are even, the rate of survival is high, the utilization rate of fish bait is high, and the growing speed is high.

The invention relates to the technology of chromosome operation, in particular to a method for inducing turbot homogenesis gynogenesis fry by using the theory that heredity inactivated sperms are used to perform artificial insemination and a fertilized ovum is restrained from cleavage through hydrostatic pressure. Sexually mature turbot parent fishes are selected, seminal fluid and spawn are collected respectively, insemination is performed on the seminal fluid and the spawn in a volume ratio of 1:100-200, and a control group is formed. The surplus seminal fluid is diluted by a precooled Ringer solution, and sperms are inactivated by ultraviolet rays. Insemination is performed on the inactivated seminal fluid and ova in a volume ratio of 1:10-20. In the 10-20 min before the first fertilized ovum crack appears, the fertilized ovum is placed in a hydrostatic press pressurizing vessel, and processing is performed by the hydrostatic pressure of 55-75 MPa for 6-8 min. Through the method, the 100% turbot homogenesis gynogenesis fry can be inducing obtained efficiently, stably and reliably.