Method for improving fugu obscurus growing characters

A technology for dark-patterned pufferfish and growth traits, which is applied in the field of aquatic biological genetics and breeding to achieve the effects of reducing breeding costs, improving breeding efficiency and preventing diseases

Inactive Publication Date: 2013-02-27
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial breeding of existing puffer puffer, breeding cost is higher, and benefit is low, has limited the development of puffer puffer breeding industry, simultaneously because in existing technology, the viscous fertilized egg hatching of puffer obscurus mostly adopts artificial Fertilized fish eggs are obtained by means of fertilization and artificial squeezing, and debonding is a topic that people pay more attention to. The existing debonding methods usually use mud or talcum powder solution for debonding treatment. The existing debonding methods usually exist Insufficient solution uniformity, uneven debonding, incomplete and other defects, which lead to a significant decrease in the hatching rate of fertilized eggs of puffer pufferfish and an increase in the rate of hatching deformities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] On April 1, 2010, 15 females and 20 males of 3-4 years old obscured puffer were selected. Place the selected obscured oriental puffer fish in a cement pond. The area of ​​the cement pond is more than 200 square meters, the water depth is 1-2 meters, and there is a complete drainage system and aeration equipment. The water in the pond is kept in a torrential state all day long. During the cultivation period, 0.01% Chinese herbal medicine composed of wild chrysanthemum, mint leaves, dried ginger, atractylodes, bitter gourd powder, and tangerine peel is added to the bait. From March 15th, lobster meat, yellow mealworm, earthworm or mussel meat, etc. Animal feed is intensively cultivated. During the cultivation period, the water temperature in the pond is controlled at 21-23°C, the dissolved oxygen in the pond is required to reach 5mg / L, the pH value is 7.5-8.5, and the transparency is 40-50cm.

[0017] After 30 days of maturation promotion, 3 mg of pituitary gland (PG) and 60...

Embodiment 2

[0020] The selected broodstock of Fugu obscurus was placed in a cement tank. After 30 days of maturation and cultivation, 2.8 mg of pituitary gland (PG) and 55 μg of luteinizing hormone releasing hormone analogue (LRH- A), divided into three injections, the interval between each injection is 12 hours, 12 hours after the third artificial injection of the drug to induce labor, the mature four obscured puffer fish were artificially harvested, using obscured puffer The semen of the male fish is dry insemination, and a total of 950g fertilized eggs are obtained. The fertilized eggs are placed in a container with a smooth inner wall, and a mixture of sodium chloride and potassium chloride 5 times the volume of the fertilized eggs is slowly poured into the container. , The mixed solution is composed of 98% sodium chloride solution with a concentration of 4‰ and 2% potassium chloride solution with a concentration of 8%. It is configured 1-1.5 hours before artificial insemination, and th...

Embodiment 3

[0023] The selected broodstock of Fugu obscurus was placed in a cement tank. After 30 days of maturation and cultivation, 3.3 mg of pituitary gland (PG) and 65 μg of luteinizing hormone releasing hormone analogue (LRH- A) Three injections, with an interval of 12 hours between each injection, 12 hours after the third artificial injection of the drug to induce labor, the mature three obscured puffer fish were artificially harvested, using obscured puffer The male fish’s semen was dry insemination, and a total of 730g fertilized eggs were obtained. The fertilized eggs were placed in a container with a smooth inner wall, and a mixture of sodium chloride and potassium chloride 5 times the volume of the fertilized eggs was slowly poured into the container. , The mixed solution is composed of 98% sodium chloride solution with a concentration of 4‰ and 2% potassium chloride solution with a concentration of 8%. It is configured 1-1.5 hours before artificial insemination, and the temperat...

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Abstract

The invention discloses a method for improving fugu obscurus growing characters and belongs to the technical field of aquatic organism genetic breeding. Three to four aged male and female fugu obscuruses are utilized as parent fishes, and the parent fishes are subjected to domestication and cultivation, artificial induced spawning, insemination, seedling stage cultivation and breeding. The method includes adding 0.01% of a Chinese herbal medicine composed of wild chrysanthemum flowers, mint leaves, dried gingers, largehead atractylodes rhizome, bitter gourd powders and dried orange peels in a bait which is fed in stage of domestication and cultivation, and feeding animal fodders such as lobsters and yellow mealworms 15 days before artificial induced spawning to perform strengthening cultivation. According to the method for improving the fugu obscurus growing characters, the Chinese herbal medicine is added in the bait which is fed in stage of parent fish domestication, the immunologic function of the parent fishes is enhanced, and the qualities of eggs and semens are improved; fertilized eggs are processed by a mixed solution of sodium chloride and potassium chloride, the abhesion effect is uniform and thorough, the fineness and transparency of fertilized egg walls after the abhesion are guaranteed; and the feeding result shows that the culturing cost is substantially reduced, the culturing cycle is shortened by about a half, and the culturing benefits are improved.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding of aquatic organisms, and in particular relates to a method for improving the growth traits of puffer puffer obscurus. Background technique [0002] Fugu obscurus (Takifugu obscurus) belongs to Fuguformes and Fuguidae. It is distributed along the coast and in the middle and lower reaches of the sea. It is commonly known as puffer. Its meat is delicious, and its fat and protein content are high. Its visceral toxins have high medicinal value, and fish skin can be used as leather material. In recent years, the scale of aquaculture has gradually expanded, and Puffer obscurus has become a new and more promising freshwater famous and high-quality cultured species. The artificial breeding of existing puffer puffer, breeding cost is higher, and benefit is low, has limited the development of puffer puffer breeding industry, simultaneously because in existing technology, the viscous fertilized eg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/10A23K1/14A23K1/00
CPCY02A40/81Y02P60/87
Inventor 吕振明李继姬李晓婷
Owner ZHEJIANG OCEAN UNIV
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