Method achieving one-generation breeding of all-female pelteobagrus fulvidraco

Abstract

The invention discloses a method achieving one-generation breeding of all-female pelteobagrus fulvidraco. The method comprises the following steps that 1, cold treatment induction is conducted on a fertilized ovum obtained by taking super-male pelteobagrus fulvidraco as a male parent and taking ordinary female pelteobagrus fulvidraco as a female parent to obtain all-male triploid pelteobagrus fulvidraco, and oogonial cells are separated from an ovary of ordinary sex-mature female pelteobagrus fulvidraco; 2, the oogonial cells are microinjected to the belly cavities of all-male triploid pelteobagrus fulvidraco larvae; 3, after sexual maturity of the larvae in the second step is achieved, sperms of a donor X chromosome are generated, insemination is conducted between the sperms of the donor X chromosome and the ovum produced by the ordinary female pelteobagrus fulvidraco, and the all-female pelteobagrus fulvidraco can be obtained. According to the method achieving one-generation breeding of the all-female pelteobagrus fulvidraco, the sperms of the X chromosome are directly generated through germ cell transplantation. Therefore, an all-female pelteobagrus fulvidraco breeding system can be rapidly established only through one-generation breeding, and all-female offspring are generated; compared with a traditional all-female pelteobagrus fulvidraco breeding method, the time can be shortened by one year; due to the fact that germ cells of the pelteobagrus fulvidraco are transplanted to the pelteobagrus fulvidraco, transplantation is conducted between the same species and variety, and a higher success rate can be obtained.

Description

technical field [0001] The invention relates to the technical field of yellow catfish cultivation, in particular to a method for breeding whole female yellow catfish in one generation. Background technique [0002] As a branch of cell engineering breeding, germ cell transplantation technology has been widely used in mammals, but fish germ cell transplantation technology was gradually established and applied after 2000. In 2003, Takeuchi et al. reported the first primordial germ cell (primordial germ cells, PGCs) transplantation system in fish, but because the number of PGCs in each donor fish embryo is small, part of it will be lost during the separation process, so the success rate is relatively low. too low to be used in production. In 2006, Okutsu et al established a novel fish spermatogonial transplantation system. In 2008, Okutsu et al. isolated spermatogonia from male trout and injected them into the peritoneal cavity of a sterile triploid salmon that had just hatche...

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Problems solved by technology

[0004] In order to solve the problem in the prior art that the production method of whole female yellow catfish still needs two generations of cultivation and the time period is long, the present invention provides a method for raising whole female yellow catfish in one generation that shortens the production cycle

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