Method for inducing and cultivating tetraploid breeding of sea urchin

A cultivation method and tetraploid technology are applied in the field of induction and cultivation of sea urchin tetraploid, which can solve the problems of short edible period, large seasonal price difference of sea urchin, short processing and marketing time, etc., so as to achieve good induction effect and improve Breeding value, the effect of prolonging the market period

Inactive Publication Date: 2008-01-16
DALIAN FISHERIES UNIVERSITY
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Problems solved by technology

[0003] Because the edible part of sea urchin is the gonad, the gonad is greatly affected by the reproductive season, and its edible period is relatively short, which is limited to before and after t

Method used

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Embodiment Construction

[0013] The induction and cultivation method of sea urchin tetraploid seed of the present invention comprises the steps:

[0014] a. Take the gallbladder out of the water tank and dry it in the shade for 0-40 minutes, inject 0.5ml-1ml of 0.5mol / L KCl solution from the periosteal membrane of the sea urchin with a syringe, wait for spawning and ejaculation, and collect bile eggs and bile sperm respectively , mixed separately and set aside;

[0015] b. Fertilize the gallbladder eggs and gallbladder collected in step a, stir continuously after fertilization, and use a water bath to control the development temperature of the fertilized eggs between 17 and 18°C;

[0016] c. 55-65 minutes after fertilization, concentrate and collect the fertilized eggs with sieve silk, put them into a closed bag, and place them in a pressure vessel of hydrostatic pressure equipment. The ambient temperature is determined to be 17-18°C;

[0017] d. Start pressurization treatment 60-70 minutes after fer...

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Abstract

The cultivation method of sea urchin tetraploid fingerlings has the steps that the urchin seedling is dried in shade for 0 to 40 minutes. The 0.5 to 2.0 ml 0.5 mol / L KCl solution is injected from the sea urchin membrana peristomialis for the spawing and spermiation. The urchine eggs and sperms are collected, and the hybrid fertilization is implemented. The developmental temperature of fertilized eggs is controlled between 17 to 18 DEG C. The fertilized eggs in concentration with a mix silk net are collected after 55 to 65 minutes of the fertilization, which are packed in a closed bag and disposed in a pressure vessel of a hydrostatic pressure equipment and the ambient temperature is identified between 17 to 18 DEG C. The pressure treatment is implemented after 60 to 70 minutes of the fertilization, in which the pressure is controlled between 70 to 80MPa and the processing time is controlled between 6 to 8 minutes. Afterwards the fertilized eggs are disposed inside a bin for hatching and stirred, and the incubation temperature is controlled between 16 to 18 DEG C. After floated, the embryos are selected with the proper aeration. The bait feeding is implemented during the larvae cultivation period. The water is exchanged 1 to 3 times everyday, and each time the quantity of water exchange is 1/3 to 2/3. The pond water is exchanged once every three days. The tetraploid fingerlings cultivated with the method can produce the triploid fingerlings, thus the time of processing and the time to the market are extended.

Description

technical field [0001] The invention relates to a method for inducing and cultivating sea urchin tetraploid. Background technique [0002] The gonads of sea urchins are rich in nutrients. In addition to high protein and amino acid content, they also contain some special physiologically active substances such as highly unsaturated fatty acids, which have the functions of preventing and treating cardiovascular diseases and anticancer effects. Today, the sea urchin species in Japan Seedling production and resource multiplication have become one of the important industries of its "cultivated fishery". France has also begun to vigorously develop the sea urchin aquaculture industry and cultivate excellent aquaculture species. After my country's self-institution introduced Ezoma dung sea urchins, the artificial breeding and breeding of sea urchins developed rapidly, and has now become a new industry in the northern coastal aquaculture breeding industry. [0003] Because the edible...

Claims

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Application Information

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IPC IPC(8): A01K61/00A61D19/00
CPCY02A40/81
Inventor 常亚青于明治曹学彬丁君王永华杨小刚
Owner DALIAN FISHERIES UNIVERSITY
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