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Cistanche deserticola composition with anti-oxidation effect

A composition and technology of cistanche, applied in organic chemistry, food science, peptide preparation methods, etc., can solve problems such as destruction, and achieve the effect of improving the value of breeding

Pending Publication Date: 2022-06-28
内蒙古大漠魂生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Appropriate free radicals can play an important role in the body, but if there are too many free radicals, when the control is broken, it will cause damage to various molecules in the human body, such as fatty acids, proteins and DNA

Method used

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  • Cistanche deserticola composition with anti-oxidation effect
  • Cistanche deserticola composition with anti-oxidation effect
  • Cistanche deserticola composition with anti-oxidation effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of Cistanche polysaccharides with antioxidant function

[0023] 1) Preparation of cistanche polysaccharide:

[0024] (1) Weigh 3 g of Cistanche deserticola powder, add 90% ethanol to soak for 2 hours, filter to get the filter residue;

[0025] (2) decoct the filter residue with water for 3 times, 2 hours each time, filter with gauze, and combine the filtrate;

[0026] (3) After evaporating and concentrating the filtrate in step (2) to 1 / 5 of the original volume, add 4 times the volume of 95% ethanol, stir well and place it in the refrigerator overnight for precipitation;

[0027] (4) Discard the supernatant, centrifuge at 4°C and 3000r / min for 10min, and collect the precipitate;

[0028] (5) The precipitate collected in step (4) was washed three times with absolute ethanol and acetone respectively, then freeze-dried, and ground to obtain a lime-like powder, ie Cistanche deserticola polysaccharide powder.

[0029] 2) Detection of free radical sc...

Embodiment 2

[0036] Example 2: Preparation of protein peptides with anti-fatigue function

[0037] 1. Preparation of protein peptides

[0038] After cleaning the fresh scallops, add 3L of purified water to the scallop meat, beat the scallop meat into a slurry with a beater, add trypsin, and enzymolysis temperature is 58°C; stir the enzymolysis reaction for 2 hours; then centrifuge with a tube centrifuge at 12000rpm to obtain enzymatic hydrolysis product.

[0039] The supernatant after the centrifugation of the enzymatic hydrolysis product is ultrafiltered with a ceramic membrane with a pore size of 6kDa; and then ultrafiltered with a membrane with a pore size of 2kDa; ) and more than 6kDa (AB-3) in three different molecular mass ranges of enzymatic hydrolyzate components; then freeze-dry the three enzymatically hydrolyzed solutions to obtain polypeptide powder.

[0040] 2. Detection of ability to scavenge free radicals

[0041] Dissolve the polypeptide powder in distilled water accordin...

Embodiment 3

[0048] Embodiment 3: the purification of protein peptide

[0049] 1) Protein peptide purification

[0050] The protein peptide of the polypeptide (AB-2) with the highest antioxidant activity after ultrafiltration was configured into a 100mg / ml solution, and was adsorbed by a macroporous adsorption resin, and purified water, 25% ethanol, 45% ethanol, 65% ethanol, 85% ethanol and 95% ethanol were eluted to obtain 5 eluted fractions. Nitric oxide synthase activity assay (Nitric Oxide Synthase Activity Assay kit, Colorimetric) was performed on the 5 eluted fractions respectively. The test results showed that 65% ethanol eluted fraction had the best activity.

[0051] The 65% ethanol elution fraction was separated again by HPLC, and the separation conditions were as follows: Instrument: Agilent1260 type high performance liquid chromatography, chromatographic column: SimoChrom ODS-BP5 μ m (4.6mm × 250mm), mobile phase: methanol and 0.1 %TFA water, flow rate: 1ml / min, detection wa...

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Abstract

The invention relates to the technical field of functional food preparation, in particular to a cistanche composition with an anti-oxidation effect. The composition comprises cistanche polysaccharide and protein peptide. Cistanche deserticola polysaccharide and protein peptide which have the anti-oxidation function are used in cooperation, the prepared composition has the anti-oxidation function, and the breeding value of cistanche deserticola can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of functional food preparation, and in particular relates to a cistanche composition with antioxidant effect. Background technique [0002] Every metabolism in which the human body interacts with oxygen will produce redox reactions, which in turn generate free radicals. Appropriate free radicals can play an important role in the body, but if there are too many free radicals, when the control is broken, it will cause damage to various molecules in the human body, such as fatty acids, proteins and DNA. These damaged molecules are more and more, and various slow inflammatory reactions occur in the human body, leading to the decline of human body function and endangering health. At the same time, free radicals will damage the elastic fibers of the skin, stimulate the formation of pigment, dry and loose skin, increase the number of spots, and aggravate aging. [0003] Cistanche deserticola is a parasitic plant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P21/06C07K7/06C07K1/34C07K1/14A23L33/125A23L33/18
CPCC08B37/0003A23L33/125A23L33/18C12P21/06C07K7/06A23V2002/00A23V2200/30A23V2250/51A23V2250/55
Inventor 裴栋邸多隆曾祥俊
Owner 内蒙古大漠魂生物科技有限公司
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