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Method for carrying out incubation on neolissochilus benasi through artificial fertilization

A technology of artificial insemination of soft-finned lip fish and artificial insemination, which can be applied in fish farming, climate change adaptation, application, etc. It can solve problems such as the same report of the same invention that has not been seen, and achieve the effects of low cost, easy operation, and high hatching rate

Inactive Publication Date: 2012-07-25
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0003] Through document retrieval, do not see identical report with the present invention

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Embodiment Construction

[0012] At about 18:00 on September 6, 2011, 12 groups of sexually mature broodstock were selected from the soft-finned fresh-faced fish collected from Xichou, Wenshan, domesticated in 2005, and injected according to the ratio of 1 female: 3 males, according to the following Methods: Artificial insemination was carried out at 9:00 on September 7, and 45,360 fertilized eggs were obtained. The hatching was completed on September 12 with the following method, and 42,000 fry were hatched, and the hatching rate was as high as 92%. The specific method is as follows:

[0013] a. Dry fertilization: Injection of gonad-mature soft fin lobsters with a luteinizing hormone-releasing hormone analogue (LHRH-A 2 ) + dioconone maleate (DOM) is a mixed oxytocic agent, the dose is 1 mg dioconone maleate + 1 μg luteinizing hormone-releasing hormone A per kilogram of body weight 2 , the injection volume of male fish is halved, and its effect time is 15 hours at a water temperature of 22°C, and art...

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Abstract

The invention discloses a method for carrying out incubation on neolissochilus benasi through artificial fertilization, which belongs to the technical field of fish product breeding. The method comprises the following steps of: injecting DOM (domperidone maleate) and LRH-A2 (lutein releasing hormone A2) into gonadosomatic neolissochilus benasi (1mg of DOM and 1 mu g of LRH per kilogram of neolissochilus benasi are injected in per kilogram of gonadosomatic neolissochilus benasi), wherein the injection volume is halved for male neolissochilus benasi, and the effect time is 15 hours at a water temperature of 22 DEG C; extruding ovums and sperms of the gonadosomatic neolissochilus benasi into a container, stirring the ovums and the sperms for 30 seconds by using chicken feather, then adding clear water at a temperature of 22 DEG C into the container (water preferably just submerges the ovums and the sperms), after stirring the obtained mixture for 20 seconds by using the chicken feather, carrying out washing three times by using clear water, then taking the obtained fertilized ovums for backup incubation; putting the fertilized ovums into an aqueous captan solution (0.15 ppm) to soak for 15 minutes, then putting the fertilized ovums into a water-filled dissecting tray and a shallow-water basin (in which the water level is preferably 3-5 cm higher than the ovums) for incubation, wherein 3000-5000 ovums with an ovum diameter of 2.2-2.8 mm are put in each basin, the position of a water-filled container is 50-80 cm higher than the dissecting tray and the shallow-water basin; sucking water by using a small-sized oxygen hose and conveying the water to the dissecting tray and the shallow-water basin to carry out incubation for 120 hours so as to get fish fries, wherein the temperature of the water is 22-23 DEG C, and the pH value of the water is 7.8-8.5. The method disclosed by the invention has the advantages of low cost, easiness in operation, and high incubation rate.

Description

Technical field: [0001] The invention relates to an artificial fertilization and hatching method for soft-finned lobster, which belongs to the technical field of fish product cultivation. Background technique: [0002] Neolissochilus benasi (Pellegrin & Chevey, 1936), formerly known as Barbodes benasi, a local name, belongs to the genus Neolissochilus in the subfamily Barbinae of Cyprinidae (Neolissochilus). Historically, the soft-fin new light lip fish was the main target of fishermen in the Yuanjiang River Basin. It was mainly distributed in Hekou, Jiangcheng, Xichou, Yuanjiang and other places in Yunnan, and it was distributed overseas in Vietnam. According to the survey results in recent years, due to the high fishing intensity, the development of cascade power stations, and the weak awareness of protection, the population of the soft-finned lip fish has declined very seriously. Counties keep larger populations. The current situation of the soft-finned lobster has att...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 潘晓赋杨君兴陈小勇李再云李列钟世腾
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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