47results about How to "Strong Survival Rate" patented technology

The invention discloses a microorganism passivator for repairing heavy metal pollution of soil and preparation and application thereof. A preparing method comprises the steps of fermentation and adsorption of strains. Specifically, bacteria solutions obtained after fermentation of bacillus megateriumvar.phosphaticum and bacillus subtilis LVLE-14 are mixed in proportion, then adsorption is conducted by means of a special adsorption carrier, and then mixing and smashing are conducted to obtain the microorganism passivator. The adsorption carrier comprises hydrolysis residues and biochar which are mixed and smashed according to a certain mass ratio. By the adoption of the microorganism passivator for repairing heavy metal pollution of soil, the content of available heavy metals in soil can be effectively reduced, and the number of microbial populations and plant yield are increased greatly. The method for repairing heavy metal pollution of soil by means of the microorganism passivator is in-situ repair, operation is easy, the cost is low, and industrialized implementation can be easily achieved; furthermore, the repairing effect is good, soil fertility can not be damaged, and the utilization rate of resources can be increased.

The invention discloses a method for ecologically culturing Rana spinosa, which comprises the following steps of culturing farm construction, culturing time, gutter way water level, culturing density, bait feeding, temperature control and disease prevention. The constructed culturing farm is basically similar to the natural ecological environment and ensures that Rana spinosa can have a good playing and activity space, the metabolism of the Rana spinosa is promoted and the Rana spinosa grow well. The gutter way water level is 2-3cm above the surface of the gutter way, so that the Rana spinosa can have moist skin and strong disease resistance. Baits are more economical and more balanced in nutrition, and the quantity of chemical drug residues in the Rana spinosa is relatively low. The method for ecologically culturing Rana spinosa has the advantages that the culturing farm is basically similar to the natural ecological environment, the playing activity space of the Rana spinosa is proper, germs are not easy to propagate, and the Rana spinosa has balanced nutrition, better growth, strong germ resistance and high survival rate.

The invention discloses a medicine for treating male oligospermia and asthenozoospermia and improving sperm quality. The medicine for treating male oligospermia and asthenozoospermia and improving sperm quality takes L-carnitine, maca, corduceps militaris, zinc-rich selenium-rich whole egg powder, vitamin C, citric acid, zizyphus jujube, deer blood, folic acid, taurine, fructose and coenzyme Q10 as active compositions, and is prepared according to a certain weight ratio. The medicine aims at the disadvantages of conventional spermatogenic medicines, and is reasonable in formula. By properly combining multiple bulk drugs and utilizing the mutual synergic effect of the bulk drugs, the treatment effect is improved, the medicine adapts to treatment requirements of complex disease conditions, adverse reaction is reduced, and the medicine is durable in treatment effect, fast in absorption, rapid in effectiveness, strong in effect, free of stimulation, safe, reliable and simple for administration, is capable of effectively promoting generation of sperms, improving the survival rate of sperms, and is an effective medicine for treating oligospermia, asthenozoospermia and sperm quality badness caused by various reasons. The total effective rate of the medicine reaches 95.6% based on 5730 examples treated by using the medicine.

The invention provides a culture substrate for dendrobium candidum tissue-cultured seedlings. The culture substrate comprises a first substrate layer, a second substrate layer, a third substrate layer and a fourth substrate layer from top to bottom sequentially, wherein the first substrate layer is formed by mixing laying of one or two kinds of crushed wood shavings and crushed bark, the second substrate layer is formed by laying of a plurality of small wood blocks, the third substrate layer is formed by laying of a plurality of long wood strips, and the fourth substrate layer is formed by laying of a plurality of crushed stones. According to the culture substrate for the dendrobium candidum tissue-cultured seedlings, the first substrate layer, the second substrate layer, the third substrate layer and the fourth substrate layer are arranged from top to bottom sequentially, and the substrate layers are made of different materials, so that the culture substrate for the dendrobium candidum tissue-cultured seedlings is low in pathogenic microorganism content and high in air permeability, seedling hardening is not required to be conducted when the substrate is used for culturing the dendrobium candidum tissue-cultured seedlings, and the survival rate of cultivation is high.

The present invention discloses a stichopus japonicus grading breeding method. It is characterized by that said method includes the following steps: making sex glands of Chinese stichopus japonicus and Chinese-Russian hybrid stichopus japonicus be simultaneously developed and matured, using shade-drying parturifacient method to respectively obtain sperm of Chinese stichopus japonicus and ovum of Chinese-Russian hybrid stichopus japonicus, making artificial insemination and incubating fertilized ovum until the laval plankton is formed, after the laval plankton is attached, utilizing conventional method to breed F1 generation of stichopus japonicus of Chinese stichopus japonicus and Chinese-Russian stichopus japonicus, then using said F1 generation stichopus japonicus as seed stichopus japonicus, accelerating the sex glands of said F1 generation stichopus japonicus and Chinese stichopus japonicus to make them be synchronously matured, making artificial insemination and breeding so as to obtain a new young of stichopus japonicus.

The invention discloses a Cyclocarya paliurus cutting seedling growing method comprising the following steps: 1, cuttingwood selecting; 2, cuttingwood processing; 3, cuttingwood planting; 4, post-planting management; the method uses specially prepared matrix, and the matrix has excellent physicochemical performance so as to provide good environment conditions for cuttingwood growth; nitration soup immersion and electric field alternative repeatedly treatment are carried out for the cuttingwood root, thus enhancing root cell splitting and differentiation capability, and improving growth vitality and anti adversity performance of the cuttingwood; combining with matrix effect and post-planting management, the novel method can largely improve cuttingwood survival rate, and the seedling is tidy and prosperous in growth vigor, thus reducing incubation time for 7-10 days when compared with same specification cutting seedlings; the method is suitable for large-area popularization plantation, and very strong in market competitiveness.

The invention discloses application of sulfated codonopsis pilosul polysaccharide to preparation of a herpes simplex virus type I resistance medicament and a preparation method of the herpes simplex virus type I resistance medicament. According to the invention, a sulphamic acid method is adopted to carry out sulphating modification on codonopsis pilosul polysaccharide, a modification method is optimized and an excellent prevention and treatment effect of codonopsis pilosul polysaccharide on the herpes simplex virus type I is found. Meanwhile, the sulfated codonopsis pilosul polysaccharide (the substituted ratio is 0.763) with the best activity is also screened out; no matter prevention administration or treatment administration, the cell survival rate of the sulfated codonopsis pilosul polysaccharide is higher than that of a virus control group (P is smaller than 0.01), the prevention effect of the sulfated codonopsis pilosul polysaccharide is equivalent to that of acyclovir (P is greater than 0.05) and the treatment effect of the sulfated codonopsis pilosul polysaccharide is superior to that of acyclovir (P is smaller than 0.05). The effect that the antiviral activity of the sulfated codonopsis pilosul polysaccharide is the highest is shown; the sulfated codonopsis pilosul polysaccharide can be used as a material for developing the novel HSV-I ( herpes simplex virus type I) resistance medicament and the material and theoretical foundation are provided for development of the novel HSV-I resistance medicament.

The invention belongs to the technical field of microbes and particularly relates to lactobacillus plantarum GL-5 with oxidation resisting activity and application thereof. The lactobacillus plantarumGL-5 with oxidation resisting activity disclosed by the invention is screened out from a home-made fermented dairy product of a herdsman's house of a Qinghai Guoluo region with an altitude of 4,000mor more, is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on November, 21, 2019 and has an accession number of CGMCCNo. 18988. The lactobacillus plantarum GL-5 has a 16S rRNA sequence shown in SEQ ID NO: 1. The lactobacillus plantarum GL-5 provided by the invention has relatively good bacteriostatic activity, and activity of enteropathogenic bacteria, i.e., staphylococcus aureus and escherichia coli can be remarkably inhibited; the lactobacillus plantarum GL-5 provided by the invention has relatively high oxidation resisting activity and can remarkably tolerate catalase; and the lactobacillus plantarum GL-5 provided by the invention has relatively high gastrointestinal fluid tolerance and cholate tolerance,and the survival rate of the lactobacillus plantarum GL-5 after culture in simulated gastric fluid and intestinal fluid is remarkably higher than that of lactobacillus rhamnosus LGG.

The invention relates to a disease-resisting and growth-promoting feed additive premix for marine fishes. The premix is prepared from the following raw materials in percentage by weight based on each ton of feed additives: 1,000 to 2,000g of 50 percent of microcapsule DL-methionine, 1,000 to 2,000g of 50 percent of microcapsule lysine hydrochloride, 800 to 1,500g of crystal taurine, 150 to 500g of 93 percent of enveloped vitamin C, 100 to 800g of 500,000 IU / g vitamin E acetic ester, 50 to 150g of 1 permillage of yeast selenium, 30 to 100g of antioxidant and 1,000 to 5,000g of 80-mesh attapulgite. The disease-resistant growth-promoting feed additive premix for the marine fishes can meet the requirements of resisting diseases and promoting growth when various marine fishes are cultivated at high density, has safety and high efficiency when used for a long time, and does not have toxic or side effect; after the various marine fishes ingest feeds with the premix for over 1 month, the serum lysozyme activity, phagocytic capacity of macrophages and serum complement activity can be obviously improved, and the survival rate of a plurality of marine fishes infected by seawater vibro harveyi can be improved at the same time; and the survival rate of cultivated marine fishes is high, the body colors of the marine fishes are bright, the anti-excitability ability of the marine fishes is high, and the feed coefficient is low.

The present invention relates to the field of Trichosanthes planting, and discloses a group cultivation method of Trichosanthes trichosanthes, comprising the following steps: (1) selecting Trichosanthes root mass to accelerate germination in a suitable environment. Polysaccharides have the obvious effect of regulating cell division and have strong resistance. The various enzymes and amino acids contained in it make the sprouts strong in rooting, and the survival rate of seedlings is higher than that of seed culture; (2) the germination to The seed buds are peeled off from the basal bud tip, which has the highest hormone level, is non-toxic, has a fast meristem speed, and is easy to cultivate; (3) The ingredients in the medium are added with various nutrients required by the seedlings as the cultivation progresses (4) The seedlings obtained by tissue culture are adapted to the external environment and can be transplanted in the field after stabilization. The method for cultivating seedlings of the group of the present invention improves the character of Trichosanthes trichosanthis, has high and stable yield, and is beneficial to the accumulation of excellent characters.

The invention provides a shipwreck escape life vest used for a ship. The shipwreck escape life vest comprises a vest body. A hanging ring is arranged at the upper end of the vest body and provided with a positioning device. Light reflective boards are arranged below the hanging ring. An inflation device is arranged on the lower portion of the vest body. A lifesaving pocket is arranged on the leftportion above the inflation device. A vital sign detector is arranged on one side of the lifesaving pocket and located in the position of the heart. A seawater purification device is arranged above the vital sign detector and connected through a rubber water pipe. A water suction port is formed in the upper end of the rubber water pipe. An oxygen supply device is arranged on one side of the seawater purification device and connected through a rubber gas pipe. An expiration pipe is arranged in the rubber gas pipe. Through the arrangement of the light reflective boards and the positioning device, rescue workers can conveniently find a drowning person in time. The shipwreck escape life vest has the beneficial effects of being convenient to wear, capable of facilitating rescue, high in victimsurvival rate and suitable for being popularized on a large scale.

The invention discloses an aspen tree planting method. The method is characterized by comprising the following steps of 1, seedling breeding field selection; 2, soil preparation; 3, branch collection and cutting slip cutting; 3, cutting slip cutting; 5, water soaking; 6, cuttage; 7, planting; 8 forest management. According to the aspen tree planting method, labor force is greatly saved, the aspen tree planting cost is lowered, the aspen tree survival rate is improved, the aspen tree planting benefits are enhanced, the aspen tree disease resistance and insect pest resistance are enhanced, and the purposes of high yield and high quality are achieved. The method is suitable for professional or non-professional planting persons for reference and usage, the method is convenient to implement, and cultivating and planting are quite convenient. Compared with a common aspen seedling breeding and planting mode, the survival rate is improved by 20%, the tree form is good, the crown breadth is obviously enlarged, the disease resistance is strong, the wind resistance is strong, economic benefits are improved by 10%, and the input management cost is lowered by 30%.

The invention provides a grafting method for big hard walnut trees of highland and cold mountainous areas. The method comprises the steps: shearing developmental branches or vegetative shoots from soft walnut trees at a later dormant stage of the soft walnut trees in a manner that tops of scions are reserved with apical buds, and carrying out wax sealing when the moisture content of the scions is 80% to 85%; selecting graft branches from the big hard walnut trees, cutting off the graft branches to leave root segments with the length of 25cm to 35cm, and forming a cut, which has the width of 6cm to 10cm and a depth reaching a woody part, in each cut section; dipping the scions with cytokinin, then, inserting the scions in the cuts of the graft branches, binding graft unions, adding plastic bags to the unions, loading the plastic bags with disinfected mountain base soil, exposing the apical buds of the scions, forming draining holes in bottoms of the plastic bags, and removing new buds and new shoots from the big hard walnut trees in time; and removing the plastic bags, the mountain base soil and binding ropes when each new shoot of each scion has the height of 24cm to 26cm and the thickness of 0.5cm to 2cm, carrying out conventional management, and removing fruits born in the same year or the ensuing year of grafting, thereby completing grafting. According to the method, the survival rate is effectively increased, and the survival rate of plants is 100%.

The invention discloses an artificial breeding method of ducklings. The artificial breeding method includes: selecting a duckling shed; feeding feed and water; controlling temperature, humidity and illumination; ventilating, cleaning and disinfecting. According to the artificial breeding method, ecological breeding is implemented, the duckling shed is disinfected and maintained dry and clean, duckling living environment is improved, proper temperature is provided for the ducklings, and the feed is fed reasonably, so that the ducklings are enabled to be high in disease resistance and survival rate and can grow quickly and healthily. The artificial breeding method is low in labor intensity and investment, quick in action, high in scientificity and operability, simple to implement, low in cost and high in applicability and is of popularization and demonstration significance.

The invention discloses a cultivation method for platycodon grandiflorum with high yield. The cultivation method comprises the following steps of field selection and soil preparation: seed pretreatment; seeding; management; removal of shade nets; pinching and nipping off of flowers; pest control. According to the method provided by the invention, seeding is performed after base fertilizer is applied, so that nutrition needed by a platycodon grandiflorum growth process is ensured at first; then shade nets are used for covering, so that raindrops are prevented from directly washing seeds, seedlings and soil, a granular structure of the soil is kept, soil hardening is avoided, and a seedling emergence rate and a survival rate are improved; the seeds are processed by the steps of sterilizing by using potassium permanganate solution, soaking into warm water, drying by a warm air machine and accelerating germination by using a germinating box, so that a germination rate of the platycodon grandiflorum is improved, and thus the platycodon grandiflorum seedlings can be stronger, the transplantation survival rate of the seedlings can be improved, the seedlings grow rapidly latterly, and product quality is good; yellow sticky traps in combination with spraying of Chinese herbal medicine are used for killing pests, so that the requirements of being environment-friendly and energy-saving are conformed, plant diseases and insect pests of the platycodon grandiflorum can be greatly relieved, pesticide residues are greatly reduced, and quality of the platycodon grandiflorum is improved.

The invention provides a grafting method for longan cut-grafting. The grafting method comprises the following steps of scion and stock selection, grafting preparation, grafting and managing after grafting. A longan seedling is a wooden root of arbor, and the root system is well developed, so that the survival rate of grafting is guaranteed. The longan seedling belongs to one same family with a high-quality longan seedling, affinity of a scion and a stock is strong, the grafting success rate is greatly improved, and the speed of growth of the grafted grafting seedling is also accelerated. The longan seedling has stronger drought-resistant ability, the survival rate of grafting can be guaranteed under a drought condition, and blossoming and fruit bearing of the grafted longan seedling can be achieved within three years. The high-quality longan grafting method of the longan seedling is simple and convenient, the survival rate is high, the coverage rate of a longan high-quality variety is improved, and blossoming and fruit bearing of the longan can be realized in advance.

The invention discloses a planting method for improving peony quality. The planting method comprises the following steps of S1, soil preparation and fertilizer applying: selecting sand soil, ploughingdeep, ridging and applying base fertilizer; S2, planting: selecting strong peony seedlings, soaking roots before planting, and watering after planting; S3, daily management: timely watering and draining, soil loosening, weeding, preventing and controlling diseases and pests, topdressing, pruning and disbudding. The planting method for improving peony quality improves stress resistance of peonies,promotes growth and development and accumulation of nutrient substances of peonies, and effectively improves yield and quality of peonies.

The invention relates to an edible melon grafting technique. The technique comprises the following steps: selecting a mother root of a wild melon and a primary branch stem of an edible melon branch to graft, thus improving disease resistance without affecting taste; removing unwanted branches of the mother root of the wild melon, remaining a main stem of 3-4cm, and cutting a slit with the depth of 1.8-2.2 on the main stem; inserting a used melon branch which is cut to form two symmetrical flat surfaces into the slit, and then wrapping the slit with a rope belt, the melon can survive after 7-10 days and the survival rate can reach over 95%. The grafting technique is characterized by simple operating method, high survival rate, good disease resistance, high yield and good plumpness of the produced seeds.

The invention relates to a high-yield cultivation method for cucumber. The method comprises the following steps of (1) soil preparation and fertilization; (2) ridging; (3) planting density; and (4) field management: (4.1) temperature management, (4.2) fertilizer and water management and (4.3) hanging vine pruning. According to the high-yield cultivation method for cucumber, the yield of the cucumber can be improved, the resistance can be strengthened and the quality can be improved; a 'needle-penetrating fast connection' grafting technology, with the unique advantages of high operability, high speed and high survival rate, for the cucumber is researched; and a technical support is provided for annually providing farmers with grafting seedlings and achieving grafting seedling industrialization.

The invention relates to bonsai cultivation methods, in particular to a making method of a tea tree bonsai. The making method comprises the following steps that firstly, a Fuyun No.6 tea tree is selected as a female parent, a Foshou or Xiaoqi tea tree is selected as a male parent, artificial hybridization is performed, and tea seeds of a single offspring plant are obtained; secondly, the tea seeds are planted in a pot; thirdly, normal temperature and humidity of soil in the pot are well kept after germination of the tea seeds; when growing to 18-22 cm, the tea tree seedlings are inspected and screened, and the tea tree seedlings integrating excellent characteristics of the male and female parents are selected for styling. According to the making method of the tea tree bonsai, firstly, the Fuyun No.6 tea tree which is high in stress resistance, wide in adaptability and high in planting survival rate serves as the female parent during artificial hybridization, and the rare tea tree variety, namely the red-shoot or green-shoot Foshou tea tree which is unique in leaf structure, rich in leaf type and diversified in leaf color and shoot and leaf color serves as the male parent. Through the making method, the problems that an original tea tree bonsai is simplex in variety, monotonous in style, low in survival rate, slow in growing trend, poor in insect resistance, prone to aging, high in management difficulty and the like are solved.

The invention discloses a cuttage planting method of subprostrate sophora. The method includes the steps of field selection and preparation, seedling selection and root promoting, field planting, illumination management, weeding, fertilization, rack erection and harvesting work. According to the planting method, by selecting the branches of 2-3-year-old subprostrate sophora as cuttings, and selecting the period from June to August as the cutting time, since subprostrate sophora stays in an exuberant growth state during the period, the branches are high in vitality, and by using the branches asthe cuttings, rooting is facilitated, and the survival rate is increased; after a rooting solution is used for soaking the cuttings, special humic soil is adopted for culture to make the cuttings generate roots, cuttage is conducted, a rooting solution is adopted for irrigation, and therefore the rooting rate of the cuttings is greatly increased. The planting method is scientific and reasonable,easy to operate, high in land utilization rate, low in production cost, suitable for large-scale systematized planting and high in practicability, subprostrate sophora cultured according to the methodis high in acre yield and survival rate, and the survival rate reaches 95% or above.

The present invention discloses a stichopus japonicus grading breeding method. It is characterized by that said method includes the following steps: making sex glands of Chinese stichopus japonicus and Chinese-Russian hybrid stichopus japonicus be simultaneously developed and matured, using shade-drying parturifacient method to respectively obtain sperm of Chinese stichopus japonicus and ovum of Chinese-Russian hybrid stichopus japonicus, making artificial insemination and incubating fertilized ovum until the laval plankton is formed, after the laval plankton is attached, utilizing conventional method to breed F1 generation of stichopus japonicus of Chinese stichopus japonicus and Chinese-Russian stichopus japonicus, then using said F1 generation stichopus japonicus as seed stichopus japonicus, accelerating the sex glands of said F1 generation stichopus japonicus and Chinese stichopus japonicus to make them be synchronously matured, making artificial insemination and breeding so as toobtain a new young of stichopus japonicus.

The invention discloses a cutting breeding technology of vine tea, which comprises the following steps: (1) selection of nursery land; (2) deep plowing and land preparation and base fertilizer application; (3) soil disinfection; Ridging and covering with plastic film; (5) green branch cutting; (6) nursery field management; (7) nursery stock transplantation; it is characterized in that the green branch cutting method and the bed making of the seedbed. Compared with the prior art, the invention can improve the survival rate and yield of vine tea plants; shorten the soaking time and improve the cutting efficiency; and is suitable for large-scale cultivation and transplantation.

The invention discloses a selective breeding method for distant hybridization offspring between megalobrama amblycephala and ancherythroculter nigrocauda. The method comprises the following steps: A, distant hybridization between megalobrama amblycephala and ancherythroculter nigrocauda: a, selecting a hybridization parent; b, crossbreeding parents: breeding the megalobrama amblycephala and ancherythroculter nigrocauda parents in different pools; c, hybridization induced spawning: selecting May to June in midstream of Yangtze River; d, cross insemination: when the parent fishes are in estrus, respectively hauling nets for fishing, selecting megalobrama amblycephala female fishes and ancherythroculter nigrocauda male fishes, artificially collecting sperms and eggs for dry insemination, and controlling the ratio of the female parent fishes to the male parent fishes to be 1: (1-2); e, hatching the hybridized fertilized eggs: debonding the fertilized eggs by adopting yellow mud or talcum powder slurry, or directly adhering the fertilized eggs to artificial fish nests, putting the fertilized eggs into a flushing type hatching tank or a hatching jar and a hatching loop for hatching, moving out the hatching equipment, and carrying out fry, fingerling and parent fish culture in a pond; and B, breeding hybrid offspring of megalobrama amblycephala and ancherythroculter nigrocauda. The method is easy to implement and simple and convenient to operate, and a new variety of excellent culter amblycephala with high yield, stress resistance, stress resistance and compact scales is obtained.

The invention discloses an anti-inflammatory antidiarrheal chicken feed and a preparation method thereof. The anti-inflammatory antidiarrheal chicken feed is prepared from the following raw materials in parts by weight: 500-600 parts of grape seed meal, 2-3 parts of a yeast extract, 8-10 parts of pawpaw protein powder, 23-25 parts of artemisia argyi, 17-21 parts of loosestrife, 14-16 parts of black sesame powder, 4-5 parts of nardostachys jatamansi powder, 65-68 parts of double-low rapeseed meal, 21-25 parts of broccoli dregs, 12-14 parts of crucian powder, 19-22 parts of sonchus oleraceus powder, 32-37 parts of water chestnut powder, 45-48 parts of gourd seed powder, 3-4 parts of garlic juice, 2-3 parts of corn germ oil, 12-13 parts of cabbage mustard dregs, 34-36 parts of black bean dregs, 4-5 parts of pomegranate vinegar, 5- parts of lithospermum, 7-8 parts of dendrobe leaves and 3-4 parts of agrimonia pilosa powder. Due to addition of components such as artemisia argyi, lithospermum, dendrobe leaves and agrimonia pilosa, the antibacterial and anti-virus properties of chicken can be effectively improved under the action of synergism of the components, meanwhile diarrhea diseases of chicken can be also effectively prevented and treated, and the survival rate of chicken can be increased.

The invention discloses a compound feed with grass as a main component. The compound feed is characterized by being processed from dry wormwood powder used as a matrix, straw powder, meal powder, cornflour and shell powder or bone meal. The compound feed comprises, by weight, 40 to 50 kg of the dry wormwood powder, 15 to 25 kg of the straw powder treated by using a biofeed preparing agent, 10 to15 kg of the meal powder, 15 kg of the corn flour and 2 to 5 kg of the shell powder. The price of the feed provided by the invention is 50% of the price of commercially-available mixed feeds, so breeding cost can be reduced, and high economic benefits can be obtained.

The invention relates to a method for cutting and propagating carnation seedlings in plug trays, and belongs to the technical field of flower cultivation. The method comprises the steps of selection of female parent seedlings, seedling picking, fringe cutting, seedling treatment, substrate preparation, hole tray preparation, cutting method, post-cutting management, finished seedlings and the like. The method of the present invention makes the root system of the carnation seedlings strong, less affected by environmental changes, fast in rooting speed and emergence speed, strong in seedlings, and the survival rate can reach 99.4%. The phenomenon of damage, and at the same time, the substrate used in the method can be reused, saving the substrate and reducing the cost. In addition, the method adopts plug trays to carry out modular cutting propagation of seedlings, which is easy to operate, convenient to water and convenient to transport.

The invention provides a culture substrate for dendrobium candidum tissue-cultured seedlings. The culture substrate comprises a first substrate layer, a second substrate layer, a third substrate layer and a fourth substrate layer from top to bottom sequentially, wherein the first substrate layer is formed by mixing laying of one or two kinds of crushed wood shavings and crushed bark, the second substrate layer is formed by laying of a plurality of small wood blocks, the third substrate layer is formed by laying of a plurality of long wood strips, and the fourth substrate layer is formed by laying of a plurality of crushed stones. According to the culture substrate for the dendrobium candidum tissue-cultured seedlings, the first substrate layer, the second substrate layer, the third substrate layer and the fourth substrate layer are arranged from top to bottom sequentially, and the substrate layers are made of different materials, so that the culture substrate for the dendrobium candidum tissue-cultured seedlings is low in pathogenic microorganism content and high in air permeability, seedling hardening is not required to be conducted when the substrate is used for culturing the dendrobium candidum tissue-cultured seedlings, and the survival rate of cultivation is high.

The invention relates to bonsai cultivation methods, in particular to a making method of a tea tree bonsai. The making method comprises the following steps that firstly, a Fuyun No.6 tea tree is selected as a female parent, a Foshou or Xiaoqi tea tree is selected as a male parent, artificial hybridization is performed, and tea seeds of a single offspring plant are obtained; secondly, the tea seeds are planted in a pot; thirdly, normal temperature and humidity of soil in the pot are well kept after germination of the tea seeds; when growing to 18-22 cm, the tea tree seedlings are inspected and screened, and the tea tree seedlings integrating excellent characteristics of the male and female parents are selected for styling. According to the making method of the tea tree bonsai, firstly, the Fuyun No.6 tea tree which is high in stress resistance, wide in adaptability and high in planting survival rate serves as the female parent during artificial hybridization, and the rare tea tree variety, namely the red-shoot or green-shoot Foshou tea tree which is unique in leaf structure, rich in leaf type and diversified in leaf color and shoot and leaf color serves as the male parent. Through the making method, the problems that an original tea tree bonsai is simplex in variety, monotonous in style, low in survival rate, slow in growing trend, poor in insect resistance, prone to aging, high in management difficulty and the like are solved.

The invention aims to overcome the shortages of bad palatability and weather limitation of green feed made of fresh wormwood, and provides a composite feed. The composite feed is developed on the basis of wormwood, and is processed and improved by adding other auxiliary ingredients into the wormwood. The composite feed for poultry and livestock is rich in nutrients, is palatable, is not affected by the weather, and can be produced all year long, and a great amount of grain is saved. The green feed made of wormwood can only be produced in the growing and mature period of wormwood (May to October), in order to utilize the wormwood with a high nutrient value, the fresh wormwood is cut off in the growing and mature period, then the fresh wormwood is dried by baking or sun immediately, the dried wormwood is pulverized into wormwood powder, the wormwood powder is taken as the matrix; auxiliary materials such as straw powder, which has been processed by a biological feed agent for preparing, meal cake, a little amount of grain, and shell / bone meal are added into the matrix to produce the composite feed, which is mainly prepared from wormwood.