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47results about How to "Strong Survival Rate" patented technology

Microorganism passivator for repairing heavy metal pollution of soil and preparation and application thereof

The invention discloses a microorganism passivator for repairing heavy metal pollution of soil and preparation and application thereof. A preparing method comprises the steps of fermentation and adsorption of strains. Specifically, bacteria solutions obtained after fermentation of bacillus megateriumvar.phosphaticum and bacillus subtilis LVLE-14 are mixed in proportion, then adsorption is conducted by means of a special adsorption carrier, and then mixing and smashing are conducted to obtain the microorganism passivator. The adsorption carrier comprises hydrolysis residues and biochar which are mixed and smashed according to a certain mass ratio. By the adoption of the microorganism passivator for repairing heavy metal pollution of soil, the content of available heavy metals in soil can be effectively reduced, and the number of microbial populations and plant yield are increased greatly. The method for repairing heavy metal pollution of soil by means of the microorganism passivator is in-situ repair, operation is easy, the cost is low, and industrialized implementation can be easily achieved; furthermore, the repairing effect is good, soil fertility can not be damaged, and the utilization rate of resources can be increased.
Owner:SHANGHAI LVLE BIO TECH

Medicine for treating male oligospermia and asthenozoospermia and improving sperm quality

The invention discloses a medicine for treating male oligospermia and asthenozoospermia and improving sperm quality. The medicine for treating male oligospermia and asthenozoospermia and improving sperm quality takes L-carnitine, maca, corduceps militaris, zinc-rich selenium-rich whole egg powder, vitamin C, citric acid, zizyphus jujube, deer blood, folic acid, taurine, fructose and coenzyme Q10 as active compositions, and is prepared according to a certain weight ratio. The medicine aims at the disadvantages of conventional spermatogenic medicines, and is reasonable in formula. By properly combining multiple bulk drugs and utilizing the mutual synergic effect of the bulk drugs, the treatment effect is improved, the medicine adapts to treatment requirements of complex disease conditions, adverse reaction is reduced, and the medicine is durable in treatment effect, fast in absorption, rapid in effectiveness, strong in effect, free of stimulation, safe, reliable and simple for administration, is capable of effectively promoting generation of sperms, improving the survival rate of sperms, and is an effective medicine for treating oligospermia, asthenozoospermia and sperm quality badness caused by various reasons. The total effective rate of the medicine reaches 95.6% based on 5730 examples treated by using the medicine.
Owner:SHANDONG XINXIAN PHARMA

Culture substrate for dendrobium candidum tissue-cultured seedlings

The invention provides a culture substrate for dendrobium candidum tissue-cultured seedlings. The culture substrate comprises a first substrate layer, a second substrate layer, a third substrate layer and a fourth substrate layer from top to bottom sequentially, wherein the first substrate layer is formed by mixing laying of one or two kinds of crushed wood shavings and crushed bark, the second substrate layer is formed by laying of a plurality of small wood blocks, the third substrate layer is formed by laying of a plurality of long wood strips, and the fourth substrate layer is formed by laying of a plurality of crushed stones. According to the culture substrate for the dendrobium candidum tissue-cultured seedlings, the first substrate layer, the second substrate layer, the third substrate layer and the fourth substrate layer are arranged from top to bottom sequentially, and the substrate layers are made of different materials, so that the culture substrate for the dendrobium candidum tissue-cultured seedlings is low in pathogenic microorganism content and high in air permeability, seedling hardening is not required to be conducted when the substrate is used for culturing the dendrobium candidum tissue-cultured seedlings, and the survival rate of cultivation is high.
Owner:INST OF DAFENG MARINE IND NANJING UNIV OF TECH

Application of sulfated codonopsis pilosul polysaccharide to preparation of herpes simplex virus type I resistance medicament and preparation method of herpes simplex virus type I resistance medicament

The invention discloses application of sulfated codonopsis pilosul polysaccharide to preparation of a herpes simplex virus type I resistance medicament and a preparation method of the herpes simplex virus type I resistance medicament. According to the invention, a sulphamic acid method is adopted to carry out sulphating modification on codonopsis pilosul polysaccharide, a modification method is optimized and an excellent prevention and treatment effect of codonopsis pilosul polysaccharide on the herpes simplex virus type I is found. Meanwhile, the sulfated codonopsis pilosul polysaccharide (the substituted ratio is 0.763) with the best activity is also screened out; no matter prevention administration or treatment administration, the cell survival rate of the sulfated codonopsis pilosul polysaccharide is higher than that of a virus control group (P is smaller than 0.01), the prevention effect of the sulfated codonopsis pilosul polysaccharide is equivalent to that of acyclovir (P is greater than 0.05) and the treatment effect of the sulfated codonopsis pilosul polysaccharide is superior to that of acyclovir (P is smaller than 0.05). The effect that the antiviral activity of the sulfated codonopsis pilosul polysaccharide is the highest is shown; the sulfated codonopsis pilosul polysaccharide can be used as a material for developing the novel HSV-I ( herpes simplex virus type I) resistance medicament and the material and theoretical foundation are provided for development of the novel HSV-I resistance medicament.
Owner:ZUNYI MEDICAL UNIVERSITY

Lactobacillus plantarum GL-5 with oxidation resisting activity and application thereof

The invention belongs to the technical field of microbes and particularly relates to lactobacillus plantarum GL-5 with oxidation resisting activity and application thereof. The lactobacillus plantarumGL-5 with oxidation resisting activity disclosed by the invention is screened out from a home-made fermented dairy product of a herdsman's house of a Qinghai Guoluo region with an altitude of 4,000mor more, is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on November, 21, 2019 and has an accession number of CGMCCNo. 18988. The lactobacillus plantarum GL-5 has a 16S rRNA sequence shown in SEQ ID NO: 1. The lactobacillus plantarum GL-5 provided by the invention has relatively good bacteriostatic activity, and activity of enteropathogenic bacteria, i.e., staphylococcus aureus and escherichia coli can be remarkably inhibited; the lactobacillus plantarum GL-5 provided by the invention has relatively high oxidation resisting activity and can remarkably tolerate catalase; and the lactobacillus plantarum GL-5 provided by the invention has relatively high gastrointestinal fluid tolerance and cholate tolerance,and the survival rate of the lactobacillus plantarum GL-5 after culture in simulated gastric fluid and intestinal fluid is remarkably higher than that of lactobacillus rhamnosus LGG.
Owner:LANZHOU UNIVERSITY

Disease-resisting and growth-promoting feed additive premix for marine fishes

The invention relates to a disease-resisting and growth-promoting feed additive premix for marine fishes. The premix is prepared from the following raw materials in percentage by weight based on each ton of feed additives: 1,000 to 2,000g of 50 percent of microcapsule DL-methionine, 1,000 to 2,000g of 50 percent of microcapsule lysine hydrochloride, 800 to 1,500g of crystal taurine, 150 to 500g of 93 percent of enveloped vitamin C, 100 to 800g of 500,000 IU / g vitamin E acetic ester, 50 to 150g of 1 permillage of yeast selenium, 30 to 100g of antioxidant and 1,000 to 5,000g of 80-mesh attapulgite. The disease-resistant growth-promoting feed additive premix for the marine fishes can meet the requirements of resisting diseases and promoting growth when various marine fishes are cultivated at high density, has safety and high efficiency when used for a long time, and does not have toxic or side effect; after the various marine fishes ingest feeds with the premix for over 1 month, the serum lysozyme activity, phagocytic capacity of macrophages and serum complement activity can be obviously improved, and the survival rate of a plurality of marine fishes infected by seawater vibro harveyi can be improved at the same time; and the survival rate of cultivated marine fishes is high, the body colors of the marine fishes are bright, the anti-excitability ability of the marine fishes is high, and the feed coefficient is low.
Owner:广东粤佳饲料有限公司

Grafting method for big hard walnut trees of highland and cold mountainous areas

The invention provides a grafting method for big hard walnut trees of highland and cold mountainous areas. The method comprises the steps: shearing developmental branches or vegetative shoots from soft walnut trees at a later dormant stage of the soft walnut trees in a manner that tops of scions are reserved with apical buds, and carrying out wax sealing when the moisture content of the scions is 80% to 85%; selecting graft branches from the big hard walnut trees, cutting off the graft branches to leave root segments with the length of 25cm to 35cm, and forming a cut, which has the width of 6cm to 10cm and a depth reaching a woody part, in each cut section; dipping the scions with cytokinin, then, inserting the scions in the cuts of the graft branches, binding graft unions, adding plastic bags to the unions, loading the plastic bags with disinfected mountain base soil, exposing the apical buds of the scions, forming draining holes in bottoms of the plastic bags, and removing new buds and new shoots from the big hard walnut trees in time; and removing the plastic bags, the mountain base soil and binding ropes when each new shoot of each scion has the height of 24cm to 26cm and the thickness of 0.5cm to 2cm, carrying out conventional management, and removing fruits born in the same year or the ensuing year of grafting, thereby completing grafting. According to the method, the survival rate is effectively increased, and the survival rate of plants is 100%.
Owner:YUNNAN ACAD OF FORESTRY

Cultivation method for platycodon grandiflorum with high yield

The invention discloses a cultivation method for platycodon grandiflorum with high yield. The cultivation method comprises the following steps of field selection and soil preparation: seed pretreatment; seeding; management; removal of shade nets; pinching and nipping off of flowers; pest control. According to the method provided by the invention, seeding is performed after base fertilizer is applied, so that nutrition needed by a platycodon grandiflorum growth process is ensured at first; then shade nets are used for covering, so that raindrops are prevented from directly washing seeds, seedlings and soil, a granular structure of the soil is kept, soil hardening is avoided, and a seedling emergence rate and a survival rate are improved; the seeds are processed by the steps of sterilizing by using potassium permanganate solution, soaking into warm water, drying by a warm air machine and accelerating germination by using a germinating box, so that a germination rate of the platycodon grandiflorum is improved, and thus the platycodon grandiflorum seedlings can be stronger, the transplantation survival rate of the seedlings can be improved, the seedlings grow rapidly latterly, and product quality is good; yellow sticky traps in combination with spraying of Chinese herbal medicine are used for killing pests, so that the requirements of being environment-friendly and energy-saving are conformed, plant diseases and insect pests of the platycodon grandiflorum can be greatly relieved, pesticide residues are greatly reduced, and quality of the platycodon grandiflorum is improved.
Owner:TONGCHENG TAIAN CHINESE HERBAL MEDICINE PLANTING PROFESSIONAL COOP

Making method of tea tree bonsai

The invention relates to bonsai cultivation methods, in particular to a making method of a tea tree bonsai. The making method comprises the following steps that firstly, a Fuyun No.6 tea tree is selected as a female parent, a Foshou or Xiaoqi tea tree is selected as a male parent, artificial hybridization is performed, and tea seeds of a single offspring plant are obtained; secondly, the tea seeds are planted in a pot; thirdly, normal temperature and humidity of soil in the pot are well kept after germination of the tea seeds; when growing to 18-22 cm, the tea tree seedlings are inspected and screened, and the tea tree seedlings integrating excellent characteristics of the male and female parents are selected for styling. According to the making method of the tea tree bonsai, firstly, the Fuyun No.6 tea tree which is high in stress resistance, wide in adaptability and high in planting survival rate serves as the female parent during artificial hybridization, and the rare tea tree variety, namely the red-shoot or green-shoot Foshou tea tree which is unique in leaf structure, rich in leaf type and diversified in leaf color and shoot and leaf color serves as the male parent. Through the making method, the problems that an original tea tree bonsai is simplex in variety, monotonous in style, low in survival rate, slow in growing trend, poor in insect resistance, prone to aging, high in management difficulty and the like are solved.
Owner:TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI

Selective breeding method for distant hybridization offspring between megalobrama amblycephala and ancherythroculter nigrocauda

The invention discloses a selective breeding method for distant hybridization offspring between megalobrama amblycephala and ancherythroculter nigrocauda. The method comprises the following steps: A, distant hybridization between megalobrama amblycephala and ancherythroculter nigrocauda: a, selecting a hybridization parent; b, crossbreeding parents: breeding the megalobrama amblycephala and ancherythroculter nigrocauda parents in different pools; c, hybridization induced spawning: selecting May to June in midstream of Yangtze River; d, cross insemination: when the parent fishes are in estrus, respectively hauling nets for fishing, selecting megalobrama amblycephala female fishes and ancherythroculter nigrocauda male fishes, artificially collecting sperms and eggs for dry insemination, and controlling the ratio of the female parent fishes to the male parent fishes to be 1: (1-2); e, hatching the hybridized fertilized eggs: debonding the fertilized eggs by adopting yellow mud or talcum powder slurry, or directly adhering the fertilized eggs to artificial fish nests, putting the fertilized eggs into a flushing type hatching tank or a hatching jar and a hatching loop for hatching, moving out the hatching equipment, and carrying out fry, fingerling and parent fish culture in a pond; and B, breeding hybrid offspring of megalobrama amblycephala and ancherythroculter nigrocauda. The method is easy to implement and simple and convenient to operate, and a new variety of excellent culter amblycephala with high yield, stress resistance, stress resistance and compact scales is obtained.
Owner:WUHAN ACADEMY OF AGRI SCI +1

Anti-inflammatory antidiarrheal chicken feed and preparation method thereof

The invention discloses an anti-inflammatory antidiarrheal chicken feed and a preparation method thereof. The anti-inflammatory antidiarrheal chicken feed is prepared from the following raw materials in parts by weight: 500-600 parts of grape seed meal, 2-3 parts of a yeast extract, 8-10 parts of pawpaw protein powder, 23-25 parts of artemisia argyi, 17-21 parts of loosestrife, 14-16 parts of black sesame powder, 4-5 parts of nardostachys jatamansi powder, 65-68 parts of double-low rapeseed meal, 21-25 parts of broccoli dregs, 12-14 parts of crucian powder, 19-22 parts of sonchus oleraceus powder, 32-37 parts of water chestnut powder, 45-48 parts of gourd seed powder, 3-4 parts of garlic juice, 2-3 parts of corn germ oil, 12-13 parts of cabbage mustard dregs, 34-36 parts of black bean dregs, 4-5 parts of pomegranate vinegar, 5- parts of lithospermum, 7-8 parts of dendrobe leaves and 3-4 parts of agrimonia pilosa powder. Due to addition of components such as artemisia argyi, lithospermum, dendrobe leaves and agrimonia pilosa, the antibacterial and anti-virus properties of chicken can be effectively improved under the action of synergism of the components, meanwhile diarrhea diseases of chicken can be also effectively prevented and treated, and the survival rate of chicken can be increased.
Owner:MAANSHAN WUGU POULTRY IND SPECIALIZED COOP

Culture substrate for dendrobium candidum tissue-cultured seedlings

The invention provides a culture substrate for dendrobium candidum tissue-cultured seedlings. The culture substrate comprises a first substrate layer, a second substrate layer, a third substrate layer and a fourth substrate layer from top to bottom sequentially, wherein the first substrate layer is formed by mixing laying of one or two kinds of crushed wood shavings and crushed bark, the second substrate layer is formed by laying of a plurality of small wood blocks, the third substrate layer is formed by laying of a plurality of long wood strips, and the fourth substrate layer is formed by laying of a plurality of crushed stones. According to the culture substrate for the dendrobium candidum tissue-cultured seedlings, the first substrate layer, the second substrate layer, the third substrate layer and the fourth substrate layer are arranged from top to bottom sequentially, and the substrate layers are made of different materials, so that the culture substrate for the dendrobium candidum tissue-cultured seedlings is low in pathogenic microorganism content and high in air permeability, seedling hardening is not required to be conducted when the substrate is used for culturing the dendrobium candidum tissue-cultured seedlings, and the survival rate of cultivation is high.
Owner:INST OF DAFENG MARINE IND NANJING UNIV OF TECH

A kind of making method of tea tree bonsai

The invention relates to bonsai cultivation methods, in particular to a making method of a tea tree bonsai. The making method comprises the following steps that firstly, a Fuyun No.6 tea tree is selected as a female parent, a Foshou or Xiaoqi tea tree is selected as a male parent, artificial hybridization is performed, and tea seeds of a single offspring plant are obtained; secondly, the tea seeds are planted in a pot; thirdly, normal temperature and humidity of soil in the pot are well kept after germination of the tea seeds; when growing to 18-22 cm, the tea tree seedlings are inspected and screened, and the tea tree seedlings integrating excellent characteristics of the male and female parents are selected for styling. According to the making method of the tea tree bonsai, firstly, the Fuyun No.6 tea tree which is high in stress resistance, wide in adaptability and high in planting survival rate serves as the female parent during artificial hybridization, and the rare tea tree variety, namely the red-shoot or green-shoot Foshou tea tree which is unique in leaf structure, rich in leaf type and diversified in leaf color and shoot and leaf color serves as the male parent. Through the making method, the problems that an original tea tree bonsai is simplex in variety, monotonous in style, low in survival rate, slow in growing trend, poor in insect resistance, prone to aging, high in management difficulty and the like are solved.
Owner:TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI
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