8 results about "Root mass" patented technology

The present application belongs to the technical field of plant tissue culture, and particularly relates to a high-efficiency rooting and seedling raising method for plant tissue culture seedlings, S1 trimming and disinfection of S2 induction liquid immersion, S3 composite substrate implantation and gradient environment regulation, S4 substrate transplanting and planting; the core is to use modified magnetic biochar composite substrate loaded with FeO nanoparticles, cooperate with the synergistic effect of microencapsulated rooting hormone and melatonin, realize in-situ integrated seedling raising through composite spectrum containing UV-A, CO pulse supply, gradient humidity and PEG osmotic pressure domestication. The present application effectively inhibits basal browning, shortens the seedling raising period, improves the rooting quality and transplanting survival rate, overcomes the pollution prejudice of magnetic nanoparticles, is suitable for woody and herbaceous plants, and can be applied to plant tissue culture seedling production on a large scale.

The invention discloses a method for improving rooting quality of eucommia ulmoides by regulating ethylene, and relates to the technical field of eucommia ulmoides breeding. The method comprises the following steps: selecting eucommia ulmoides tissue culture clonal elongation buds as a material; inoculating the elongated buds into an adventitious root one-step induction (O treatment) culture medium; in addition, performing two-step rooting induction (T treatment) on the elongated buds: pre-culturing for 7 days, and then transferring into a hormone-free culture medium; differential genes of an ethylene signal transduction pathway and a biosynthesis pathway on the twelfth day and the twenty-first day of O treatment and T treatment are analyzed, and it is determined that ethylene may be a main factor affecting the two rooting modes; the mutual regulation effect of NAA and ethylene is further verified, and the development of adventitious roots of eucommia ulmoides is regulated by indirectly regulating the generation of ethylene. The method has the beneficial effects that the efficient eucommia ulmoides rooting method which is high in rooting efficiency, robust in root system, few in callus formation and clear in mechanism is developed, and the method has important significance in research on promoting adventitious root development of eucommia ulmoides.

The application discloses a cutting treatment method for improving the rooting rate of Buddleja officinalis, and belongs to the technical field of plant cutting propagation. The method comprises the following steps: S1, cutting pretreatment: placing the base of Buddleja officinalis cutting in an ultrasonic generator for ultrasonic pretreatment; S2, rooting agent treatment: immersing the base of the pretreated cutting in a composite rooting agent solution, wherein the composite rooting agent comprises naphthaleneacetic acid, indolebutyric acid and vitamin B1; S3, cutting and maintenance: cutting the treated cutting in a special substrate, and performing post-cutting management. The application improves the cell permeability through ultrasonic treatment, promotes root growth in cooperation with the composite rooting agent with a specific formula, and optimizes the rhizosphere environment in cooperation with the special substrate, thereby improving the rooting rate, the number and quality of the root system of Buddleja officinalis, solving the problems of low rooting rate and poor rooting quality of the existing cutting method, and having the advantages of simple operation, good rooting effect and high seedling raising efficiency.

A plant support structure (e.g., a grow tower) comprises a spine and grow sites (e.g., pots) coupled to the spine. Each grow site may be configured to hold a root mass of a plant, such as a micro dwarf tomato. Each grow site may be disposed above another grow site. A ratio of a width of the spine to a width of the grow sites may be less than 1.0. A harvesting system may comprise multiple plant support structures, and a superstructure from which the structures are suspended and conveyed along a grow line to a harvesting site.

The invention discloses a superficial layer liquid static culture method for tissue culture seedlings of bamboo reeds, and belongs to the technical field of plant biologication.The method comprises the following steps that S1, explant collection and sterilization treatment are conducted, specifically, new tender stem segments with axillary buds of the bamboo reeds are selected as the explants, and cleaning and disinfection are conducted; s2, cluster bud induction: inoculating the disinfected explant into a superficial layer liquid cluster bud induction culture medium for culture, and inducing cluster buds; s3, multiplication culture: transferring the cluster buds induced in the step S2 into a superficial layer liquid multiplication culture medium for culture, and carrying out multiplication; s4, rooting culture: transferring the multiple shoots proliferated in the step S3 into a superficial layer liquid rooting culture medium for culture, and inducing rooting; and S5, transplanting: transplanting the rooted seedlings in the step S4 into a hole tray, and carrying out seedling hardening and growth. The superficial layer liquid culture method is remarkably superior to a traditional solid culture method in the aspects of proliferation efficiency, rooting quality, transplanting survival rate and the like, the working efficiency can be improved, and the production cost can be reduced.

Disclosed are methods of enhancing plant growth, comprising treating seed at least one month prior to planting with an effective amount of a plant signal molecule, wherein upon harvesting the plant exhibits at least one of increased plant yield measured in terms of bushels / acre, increased root number, increased root length, increased root mass, increased root volume and increased leaf area, compared to plants harvested from untreated seed, or compared to plants harvested from seed treated with the signal molecule just prior to or within a week or less of planting.

This invention provides a method for inducing rooting in Vaccinium tissue culture seedlings based on expanded clay granules, comprising the following steps: S1, preparing an aminated expanded clay granule-endophytic bacteria fermentation composite carrier; S2, preparing an expanded clay granule-gel composite substrate containing the aminated expanded clay granule-endophytic bacteria fermentation composite carrier; S3, after pretreatment of Vaccinium adventitious buds, aseptically inoculating them into the expanded clay granule-gel composite substrate, and culturing them at 22-24℃ and a light intensity of 1500-2000 Lx for 35-45 days to induce rooting; S4, after hardening off for 3-5 days, transplanting them into a mixed substrate of peat moss and perlite for acclimatization and cultivation for 15-20 days. This invention, using an aminated expanded clay granule-endophytic bacteria fermentation composite carrier and an expanded clay granule-gel composite substrate to induce rooting, can promote root primordia differentiation, stabilize the root growth microenvironment, standardize culture conditions to ensure stable rooting, and harden off for seedlings to enhance their stress resistance and adaptability, effectively improving the rooting quality and transplant survival rate of Vaccinium tissue culture seedlings, providing technical support for their efficient seedling cultivation.

The application discloses an out-of-bottle rooting method for bamboo reed tissue culture seedlings, and comprises the following steps: S1, seedling training: carrying out seedling training on the sterile bamboo reed tissue culture seedlings under the condition that the bottle is not opened; S2, induction treatment: transplanting the tissue culture seedlings after the seedling training in S1 into a container filled with a transplanting substrate, and using a naphthaleneacetic acid (NAA) solution with a concentration of 300-700 mg / L to irrigate the substrate to perform rooting induction; and S3, rooting management: placing the transplanted seedlings in a controllable environment for management, keeping high humidity and light shielding conditions, and until rooting is achieved. The application organically combines seedling training, optimized substrate and hormone treatment, and effectively overcomes problems such as a long rooting period, poor rooting quality, low transplanting survival rate and the like of the bamboo reed test tube seedlings in the bottle. The method has simple steps and low cost, can significantly promote the bamboo reed test tube seedlings to rapidly form developed and healthy root systems outside the bottle, the rooting rate can reach 100%, the seedlings are healthy and strong, the transplanting adaptability is extremely high, and the method is suitable for large-scale commercial seedling production of the bamboo reed.