The invention discloses a method for quickly and efficiently propagating
Callicarpa japonica, and belongs to the technical field of
Callicarpa japonica propagation and cultivation. The propagation method comprises the following steps: (1) explant preparation: selecting tender shoots, which just germinates in spring,of
Callicarpa japonica as explants; (2) aseptic treatment of the explants: stripping all tissues except true leaves on the tender shoots for disinfection treatment; (3)
primary culture: transferring the disinfected tender shoots into a
primary culture medium; (4) subculture: after new contact surfaces are exposed, transferring the whole shoots into a subculture medium, and transferring for three times to form clumpy buds; and (5) integrated culture of
seedling strengthening and rooting outside a
bottle:
cutting subculture cluster buds with strong growth vigor into single buds, putting a matrix and a gel
nutrient sheet into hole trays, inserting the single buds to be
cut into the matrix by about 1 cm by using
tweezers, then thoroughly watering the single buds by using a rooting solution, and completely covering the single buds by using a transparent film. The problems that the rooting quality of the Callicarpa japonica is poor and the
survival rate of
tissue culture seedlings is low due to improper matching of a
tissue culture method and a culture medium are solved, meanwhile, the culture period is shortened, and the culture cost is reduced.