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A kind of method for rapid and efficient propagation of Japanese purple pearl
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A kind of purple bead, fast technology, applied in horticultural methods, botanical equipment and methods, planting substrate, etc., can solve the problem of improper coordination between tissue culture method and culture medium, poor rooting quality of Japanese purple bead, and low survival rate of tissue culture seedlings To achieve the effect of improving the quality of tissue culture seedlings, promoting rapid propagation and promotion, and shortening the seedling cycle
Active Publication Date: 2022-06-07
CHONGQING ACADEMY OF FORESTRY SCI
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[0005] In view of this, the purpose of the present invention is to provide a method for rapid and efficient propagation of Japanese purple pearls, to solve the problems that the tissue culture method and the culture medium are improperly matched, resulting in poor rooting quality of Japanese purple pearls and low survival rate of tissue cultured seedlings
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Embodiment 1
[0039] Before carrying out the rapid and efficient propagation of Japanese purple bead, the gel nutritional tablet used in the stage of strong seedling and rooting culture was prepared, and the preparation method was as follows:
[0040] First, weigh 30 g of sago starch, 10 g of sodium lignosulfonate, 15 g of pectin, 5 g of sodium trimetaphosphate, and 8 g of potassium dihydrogen phosphate.
[0041] Preparation:
[0042] Modification of sago starch: make sago starch into starch milk with a mass fraction of 45%, add sodium lignosulfonate, stir at a high speed for 15 minutes at a rate of 900 r / min, and stand at room temperature for 12 hours overnight;
[0043] Gelatinization modification: stir the modified sago starch after standing still, heat it in a water bath for 20 minutes at a water temperature of 40°C, add pectin and stir for 15 minutes, stop heating after stirring, and make a saturated solution of potassium dihydrogen phosphate , add starch solution and stir evenly, the...
Embodiment 2
[0046] First, weigh 40 g of sago starch, 12 g of sodium lignosulfonate, 16 g of pectin, 8 g of sodium trimetaphosphate, and 10 g of potassium dihydrogen phosphate.
[0047] The preparation method is the same as that of Example 1.
Embodiment 3
[0049] First, weigh 50 g of sago starch, 15 g of sodium lignosulfonate, 18 g of pectin, 10 g of sodium trimetaphosphate, and 12 g of potassium dihydrogen phosphate.
[0050] The preparation method is the same as that of Example 1.
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Abstract
The invention discloses a method for rapid and high-efficiency propagation of Japanese purple pearl, belonging to the technical field of Japanese purple pearl propagation and cultivation. The propagation method is as follows: (1) Explant: select the purple purple bud that has just germinated in spring as the explant (2) aseptic treatment of explants: peel off all tissues other than the true leaves on the shoots and carry out disinfection treatment; (3) primary culture: transfer the sterilized tender shoots to the primary culture medium; (4) subculture : after exposing the new contact surface, the whole buds are transferred to the subculture medium, and transferred 3 times to form cluster buds; (5) integrated cultivation of strong seedlings and roots outside the bottle: the vigorous subculture buds are cut into single buds, and the Put the substrate and gel nutrient sheet into the hole tray, insert the single bud to be cut into the substrate about 1cm with tweezers, and then pour it with the rooting liquid, and the transparent film will cover it completely; solve the problem of improper cooperation between the tissue culture method and the culture substrate, resulting in Japanese The rooting quality of purple pearl is not good, and the survival rate of tissue culture seedlings is low. At the same time, the cultivation cycle is shortened and the cultivation cost is reduced.
Description
technical field [0001] The invention relates to the technical field of Japanese purple bead propagation and cultivation, in particular to a method for rapid and efficient propagation of Japanese purple bead. Background technique [0002] Japanese purple pearl, scientific name: Callicarpa japonica Thunb.var.japonica, Verbena is a deciduous shrub, English name beautyberry, known as a beautiful berry. The inflorescence is cymose, lavender, the branches are slender, the plant shape is fluffy, the autumn fruit is purple, shiny, very gorgeous, and each node is born, continuous in clusters, and does not fall for a long time. It has high ornamental value. Studies have shown that the leaf of violet leaves also has good medicinal value. The extract of violet fruit has a variety of excellent antioxidant components, which can inhibit the formation of pigmentation and fight skin aging. [0003] The wild species of J. japonica are widely differentiated, and horticultural species are now ...
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