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Quick cultivation method of big seedlings of Tinospora sagittata

A cultivation method and technology of green cattle gallbladder are applied in the field of rapid cultivation of large green cattle gallbladder seedlings, which can solve the problems of low natural seed setting rate, small number of seeds, slow growth, etc. , the effect of avoiding infection

Inactive Publication Date: 2019-04-19
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of natural growth of green birch gall, the cycle from seedling growth to medicinal material is as long as 4 years, and the time for flowering and fruiting is about 3 years. For a long time, the collection of green bile gall has relied on digging wild products as medicine. However, artificial cultivation has not yet been substantially carried out. Due to the fact that only digging is not planted, and the growth is slow, wild resources are depleting day by day. Artificial cultivation and tending are imperative.
At present, the breeding of Tinospora is mainly through tuber division propagation, cutting seedling breeding, and seed propagation. Due to dioecious plants of Tinospora, pollination is greatly affected by the environment, and the natural seed setting rate is low. It is found in resource investigation and introduction that There are far more male plants than female plants (about 50:1), resulting in very low seed setting rate of Tinospora, less seeds, and obvious dormant period of seeds, low germination rate, and low survival rate of Tinospora cutting propagation , the division propagation of Tinobilis has always faced the problems of slow germination, low germination rate and low survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Seed selection: During September every year, select healthy, no pests and diseases, and a single green ox gall tuber with a maximum diameter of 0.5-2cm for seeding;

[0028] Disinfection and hormone treatment: Cut off the selected green ox gall tuber from the connecting bridge to become a single root piece, soak it in 1000 times carbendazim dilution for 2 minutes, take it out and dry it, and then use 2mg / L 6-BA cell Soak the cleavage for 2 hours, take it out and dry it in the air to obtain the aseptic root block, and set it aside;

[0029] Pregermination: Build a rectangular germination pool indoors with adobe, the pool wall is 60 cm high, and lay 10 cm thick sun-dried straw, 5 cm thick rice bran, and 3 layers on the bottom of the germination pool and the inner walls of the four sides from bottom to top. Grass paper, choose sunny and warm weather, put the aseptic root block layer by layer in the germination pond, the number of stacked layers is 2 layers, and the thickne...

Embodiment 2

[0037] Seed selection: During September every year, select healthy, no pests and diseases, and a single green ox gall tuber with a maximum diameter of 0.5-2cm for seeding;

[0038] Disinfection and hormone treatment: Cut off the selected green ox gall tuber from the connecting bridge to become a single root piece, soak it with 1000 times carbendazim dilution for 3 minutes, take it out and dry it, and then use 2mg / L 6-BA Soak in cytokinin for 2 hours, take it out and dry it in the air to obtain aseptic root pieces, and set aside;

[0039] Germination: Build a rectangular germination pool indoors with bricks, the pool wall is 60 cm high, and lay 10 cm thick sun-dried straw, 5 cm thick rice bran, and 4 layers on the bottom of the germination pool and the inner walls of the four sides from bottom to top. Straw paper, choose sunny and warm weather, put the aseptic root block layer by layer in the germination pool, the number of stacked layers is 3 layers, and the thickness is 9.8cm...

Embodiment 3

[0047] Seed selection: During September every year, select healthy, no pests and diseases, and a single green ox gall tuber with a maximum diameter of 0.5-2cm for seeding;

[0048] Disinfection and hormone treatment: Cut off the selected green ox gall tuber from the connecting bridge to become a single root piece, soak it in 1000 times carbendazim dilution for 2 minutes, take it out and dry it, and then use 2mg / L 6-BA Soak in cytokinin for 2 hours, take it out and dry it in the air to obtain aseptic root pieces, and set aside;

[0049] Germination: Build a rectangular germination pool indoors with bricks, the pool wall is 60 cm high, and lay 10 cm thick sun-dried straw, 5 cm thick rice bran, and 3 layers on the bottom of the germination pool and the inner walls of the four sides from bottom to top. Grass paper, choose sunny and warm weather, put the aseptic root block layer by layer in the germination pool, the number of stacked layers is 1 layer, the thickness is 5cm, and aft...

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Abstract

The invention discloses a quick cultivation method of big seedlings of Tinospora sagittata and relates to the field of planting of Tinospora sagittata. The method herein includes the steps of selecting seeds, disinfecting, carrying out hormone treatment, accelerating germination, treating a culture medium, leveling a land, treating accelerated germination root mass, growing seedlings, and managingafter seedling growth. Healthy tubers of Tinospora sagittata are used as seed tubers which are disinfected twice so that bacteria in the tubers of Tinospora sagittata can be killed first, infectionsin the cultivation process are avoided, and the survival rate can be increased to certain degree; in addition, the tubers of Tinospora sagittata are treated with 6-BA cytokinin and ABP rooting powder,germination of dormant buds or adventitious buds of Tinospora sagittata is promoted, rooting of the buds is promoted, and germination rate and survival rate can be further increased; the hormone treatment helps promote the tubers of Tinospora sagittata to germinate in advance, so that the cycle of Tinospora sagittata seeds to grow from seedlings to medicinal material is shortened.

Description

technical field [0001] The invention relates to the technical field of green boll gall planting, in particular to a rapid cultivation method for big seedlings of green boll gall. Background technique [0002] Tinospora sagittata (Oliv.) Gagnep. is a vine of Fangjiaceae, which is mainly produced in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other provinces and regions. Jin Guolan has been recorded in the previous editions of "Chinese Pharmacopoeia". It has the effects of clearing away heat and detoxification, relieving sore throat and relieving pain. Regional precious Chinese herbal medicine. Modern pharmacological studies have shown that Lavender has obvious anti-inflammatory and analgesic, anti-bacterial, anti-tumor, hypoglycemic, anti-stress and detoxification effects, and is also known as a natural drug that can replace antibiotics because of its anti-virus, The special effect of anti-tumor has been devoted to the research and development of new drugs for many years, a...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08A01G22/00
CPCA01C1/00A01C1/08A01G22/00
Inventor 周益权谭小梅叶成娟潘瑞
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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