A kind of stem segment induction plant regeneration medium and its tissue culture rapid propagation method

A technique of regeneration medium and rooting medium, which is applied in the field of regeneration medium for stem segment induction of Trichosanthes and its tissue culture rapid propagation, can solve the problems of low rooting rate and poor rooting quality of Trichosanthes tissue culture, and achieve low cost , high rooting rate and simple formula

Active Publication Date: 2022-06-28
山西省林业和草原科学研究院
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of low rooting rate and poor quality of rooting in tissue culture of the present invention, the present invention provides a culture medium for inducing plant regeneration of the stem segment of the hairy tree and its tissue culture rapid propagation method

Method used

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  • A kind of stem segment induction plant regeneration medium and its tissue culture rapid propagation method
  • A kind of stem segment induction plant regeneration medium and its tissue culture rapid propagation method
  • A kind of stem segment induction plant regeneration medium and its tissue culture rapid propagation method

Examples

Experimental program
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Effect test

example 1

[0037] Example 1: Axillary bud induction test

[0038] 1. Test materials: The test materials are collected from the four-year-old Phytophthora seedlings, and the young stems that grow vigorously and are free from diseases and insect pests or the stems after hydroponic cultivation are selected as explant materials.

[0039] After washing the harvested stems of the hairy stems, they were sterilized with 75% alcohol for 30 s on the ultra-clean workbench, rinsed with sterile water for 3 times, and sterilized with 0.1% HgCl sterilant. 2 The solution is sterilized for 4 to 5 minutes. After sterilization, rinse with sterile water for 5 to 6 times, cut into 1.5-1.8 cm stem segments, and each stem segment has at least one axillary bud, ready for inoculation.

[0040] Experimental design: A single factor completely randomized design was adopted, with 20 bottles per treatment, 1 explant was inoculated in each bottle, and repeated 3 times.

[0041] 2. The period of explant collection: In...

example 2

[0044] Example 2: Test tube plantlet proliferation and culture test

[0045] 1. Combination of BA and IBA: The induced axillary buds were transferred to MS and improved MS basic medium, and different concentrations of BA and IBA were added. After 4 weeks, the proliferation rate and proliferation coefficient of the test-tube plantlets were counted, and the effect of the combination of BA and IBA was studied. Effects on proliferation of capillary in vitro plantlets.

[0046] 2. BA and IBA, GA 3 Combination: Transfer the induced axillary buds to MS and modified MS basic medium, and add different concentrations of BA, IBA, GA 3 After 4 weeks, the proliferation rate and proliferation coefficient of the test tube seedlings were counted, and different concentrations of BA, IBA, and GA were studied. 3 Effects of combinations on the proliferation of capillary in vitro plantlets.

[0047] 3. Culture conditions: the culture temperature is 25 ± 2°C, the light intensity is 2500 lx ~ 300...

example 3

[0048] Example 3: Test tube seedling rooting culture test

[0049] 1. Test material: use the test-tube seedlings with a height of > 2 cm as the test material, and transfer them to the rooting medium for rooting culture.

[0050] 2. Experimental design: 1 / 2MS and 1 / 4MS were used as the basic medium, and different concentrations of IBA or NAA (0.05, 0.1, 0.2, 0.3, 0.5, 1.0 mg·L -1 ), the basic medium without any auxin was used as the control. After four weeks, observe and record the rooting situation, count the rooting rate and the number of roots, and study the influence of NAA or IBA on the rooting of test-tube seedlings.

[0051] A single-factor completely randomized design was adopted, with 20 bottles per treatment and repeated 3 times.

[0052] 3. Culture conditions: the culture temperature is 25 ± 2°C, the light intensity is 2500 lx ~ 3000 lx, and the light time is 14 ~ 16 h / d; all media are added with 15 g L of sucrose -1 , agar 6.5 g·L -1 .

[0053] 4. Data statisti...

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Abstract

The invention belongs to the technical field of tissue culture and breeding of forest trees. In order to solve the current problems of low rooting rate and poor rooting quality in tissue culture of Trichosanthes, it provides a culture medium for inducing plant regeneration with stem segments of Trichosanthes and a tissue culture rapid propagation method thereof. The improved MS is used as the basic medium, and the young stems of the hairy stems are used as the explants to induce axillary buds, induce adventitious buds with sterile seedlings, and then induce the regenerated plants to take root from the adventitious buds. The improved culture medium is selected for the first generation, second generation and rooting culture. The culture time is short and the culture process is simple, which improves the reproduction efficiency of the hairpin. The survival rate of induced axillary buds is over 90%, the proliferation coefficient is 8.1-9.5, and the rooting rate is 85%. Above; can quickly obtain the test-tube seedlings of the same genetic character. The use of tissue culture and rapid propagation technology for explant culture can not be affected by seasonal climate changes and natural disasters, the regeneration cycle is short, the reproduction coefficient is high, and the rooting rate is high, which provides technical support for large-scale industrial production of seedlings.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and breeding of forest trees, and in particular relates to a culture medium for inducing plant regeneration of hairy stem segments and a method for tissue culture and rapid propagation thereof. Background technique [0002] According to statistics, China's domestic oil reserves can only be exploited for 17 years, and the world's oil will be exhausted within 30 to 40 years. Facing the dwindling oil reserves and increasing oil consumption, the energy crisis has become the focus of the world's attention. Energy security It is a very urgent and real problem we are facing. The development of petroleum alternative energy and renewable energy has become an inevitable choice of energy strategy. Trichosanthes is an energy tree species with great development potential. [0003] Mao 梾 ( Cornus walteri Wanger.), also known as black starling, car beam wood, etc., belongs to the genus of Cornus genus,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张娜刘劲张春媛杨飞杨俊鸾王建义马佳琳贾迎春陈春李林英梁爱军贺义才程丽芬张彩红张腾飞
Owner 山西省林业和草原科学研究院
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