A method for inducing regenerated plants from the first internode of water spinach stalks

A technology for regenerating plants and water spinach, which is applied in the field of plant cultivation and can solve the problems of poor rooting quality and low germination rate

Active Publication Date: 2021-09-14
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the present invention provides a method for inducing regenerated plants from the first internode of the water spinach stalk, which provides a new explant type for the regeneration of water spinach plants, and solves the problems of low germination induction rate, Problems with poor rooting quality

Method used

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  • A method for inducing regenerated plants from the first internode of water spinach stalks
  • A method for inducing regenerated plants from the first internode of water spinach stalks
  • A method for inducing regenerated plants from the first internode of water spinach stalks

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Embodiment 1

[0019] Embodiment one: a kind of method of inducing regeneration plant by the first internode of water spinach stalk, comprises the following steps:

[0020] 1. Cultivation of sterile vaccines

[0021] First, select plump water spinach seeds, soak them in 55°C distilled water for 30 minutes, then soak them in 75% alcohol for 30 seconds on an ultra-clean workbench, and rinse them twice with sterile water for 2 minutes each time. Soak with 20% sodium hypochlorite solution for 20min, rinse with sterile water 6 times, and finally inoculate in the seedling medium for cultivating water spinach aseptic seedlings, and the seedling medium is the plant tissue culture antibacterial agent with 0.05% content added on the MS basic medium PPM, cultivated in the dark until the seeds are white, then cultured under normal light, and wait for the cotyledons to fully unfold for use (about 7-10 days). The growth of the aseptic seedlings is in good condition, and the pollution rate is lower than 3%...

Embodiment 2

[0028] Example 2: Effects of Different Germination Inducing Mediums on the Induction of Adventitious Buds in the First Internode of Water Spinach Stem

[0029] The first internode of spinach stalks was placed on the germination induction medium with different concentrations of hormones, as shown in Table 1. With the increase of 6-BA concentration, the induction rate showed a trend of increasing first and then decreasing, and the concentration of 6-BA was 2mg / L, the induction rate of adventitious buds decreased, and high concentration of 6-BA may have an inhibitory effect on adventitious buds. With the increase of IAA concentration, the induction rate of adventitious buds decreased. It can be seen that low concentration of IAA can promote the formation of adventitious buds, but high concentration of IAA can inhibit it. When the concentration of 6-BA was 1.0mg / L and the concentration of IAA was 0.1mg / L, the induction rate was the highest, which was 20%. As shown in Table 2, co...

Embodiment 3

[0035] Embodiment three: the influence of different rooting medium on the rooting culture of water spinach

[0036] In this experiment, the effect of different concentrations of auxin NAA on the rooting of spinach adventitious shoots was studied. The induced adventitious shoots were excised from the base and inoculated on 1 / 2 MS and MS basic medium containing different concentrations of NAA. Implants were observed for rooting after 10 days. According to Table 3, when no phytohormone is added, adventitious buds can also grow adventitious roots, thereby forming a complete plant, but it takes a long time and the number of adventitious roots is small. After adding NAA, the rooting time is shortened and the number of roots With the increase of NAA concentration, the rooting number showed a trend of first increasing and then decreasing. At the same time, compared with the MS basic medium, the fibrous roots obtained on the 1 / 2MS basic medium are longer and the number of roots is mor...

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Abstract

The invention discloses a method for inducing regenerated plants from the internodes of water spinach stalks. % content PPM; cut the first internode of sterile seedling stems as explants, and inoculate them on the germination induction medium to induce adventitious buds. The germination induction medium is MS+1.0mg / L 6‑BA+0.1mg / L IAA; cut off the adventitious buds from the base as explants, inoculate them into the rooting medium for rooting induction, the rooting medium is 1 / 2MS+0.1mg / L NAA; when the regenerated seedlings of water spinach grow well, harden the seedlings for 1 day Finally, the plants were transplanted into a nutrient pot containing Pin's peat soil for cultivation, the temperature was 24° C., the light intensity was 2500 lux, and the light time was 16 h / d. Using the first internode of the stalk as an explant, and cultivating with a special germination induction medium and rooting medium, the germination induction rate and rooting quality are significantly improved. The in vitro regeneration system constructed by the present invention lays a solid foundation for the subsequent research on the genetic transformation of water spinach. base.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for inducing regeneration of plants from the first internode of water spinach stalks. Background technique [0002] Water spinach (Ipomoea aquatica Forsk), also known as water spinach, belongs to the herbaceous plant of the genus Sweetpotato in the family Convolvulaceae, which can be both aquatic and terrestrial. Water spinach has high nutritional value and is a popular vegetable. It can be fried, cold and dressed with soup. In addition, water spinach also has the effects of clearing heat, detoxifying, cooling blood, diuresis and laxative, and purifying the water environment. The root system of spinach is shallow, the fibrous roots are well developed, the stems are sprawling, round and hollow, soft, green or lavender, the stems are 1-2 cm thick, the stems have nodes, the internodes are 3.5-5 cm long, and the longest can reach 7 cm In addition to the axillary buds, adve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 汪志伟郝园园舒黄英杨壮周燕成善汉朱国鹏
Owner HAINAN UNIV
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