The medium and use for rapidly promoting the proliferation and rooting of ash shoot tips at the same time

A technology of culture medium and white wax, which is applied in the direction of tissue culture, application, and microorganisms. It can solve the problems of time-consuming and labor-intensive planting, poor growth of seedlings, and low rooting rate of cuttings. cycle, good effect

Active Publication Date: 2022-04-12
宁波和养生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common propagation methods of white wax are mainly seedlings and cuttings. Sowing seedlings is time-consuming and laborious, and the seedlings grown are not good and uneven
Cutting seedlings have not been implemented on a large scale, the main reason is that the rooting rate of cuttings is extremely low

Method used

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  • The medium and use for rapidly promoting the proliferation and rooting of ash shoot tips at the same time
  • The medium and use for rapidly promoting the proliferation and rooting of ash shoot tips at the same time
  • The medium and use for rapidly promoting the proliferation and rooting of ash shoot tips at the same time

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Embodiment Construction

[0017] A culture medium that rapidly promotes the proliferation and rooting of white wax shoot tips, the culture medium is: MS+6-BA0.2mg / L+TDZ 0.01mg / L+NAA 0.1mg / L+20g / L sucrose+agar 7.5g / L.

[0018] A kind of application of described culture medium in the cultivation of white wax seedlings comprises the following steps:

[0019] (1) Cultivation of white wax aseptic vaccine

[0020] Sterilize the young stem tip and stem section of white wax with 75% alcohol for 35 seconds, then sterilize with 0.1% mercury chloride for 7 minutes, then rinse with sterile water for 3 to 4 times, and inoculate it in the primary medium MS + 6 -BA 3mg / L + NAA 0.5mg / L+ sucrose 30g / L + agar 7.5g / L, after 15 days, subculture the surviving shoot tips and stem segments to MS + 6-BA 2mg / L + IBA 0.2mg / L+ sucrose 20g / L + agar 7.5g / L medium, placed in a culture room with a temperature of 26°C, a light intensity of 2000 lux, and a daily light time of 16 hours to obtain aseptic white wax seedlings;

[0021...

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Abstract

The invention discloses a culture medium and its application for rapidly promoting the proliferation and rooting of ash shoot tips at the same time. L sucrose + agar 7.5g / L. The culture medium of the present invention can quickly complete multiplication, strong seedlings and rooting at the same time. The whole growth process is 30 days, and the white wax test-tube plantlets with a multiplication coefficient ≥ 1.6, an average seedling height of 3.62 cm and a rooting rate of 86.27% can be obtained, and the obtained test-tube plantlets are of high quality , the quality and quantity of rooting are good, which reduces the one-time subcultivation and reduces the chance of contamination of test-tube seedlings. The seedling cultivation period of the white wax test-tube plantlet improves the propagation speed and the reproduction coefficient of the white wax test-tube plantlet.

Description

technical field [0001] The invention relates to a culture medium for white wax seedlings and its application. Background technique [0002] White wax is salt-alkali-resistant, has strong stress resistance, and has a wide range of adaptability. It is a pioneer tree species for the development and utilization of saline-alkali land. The common propagation methods of white wax are mainly seeding and cutting, which is time-consuming and laborious, and the growth of the cultivated seedlings is not good and uneven. Cutting seedling cultivation has not yet been implemented on a large scale, mainly because the rooting rate of cuttings is extremely low. The technique of tissue culture breeding seedlings has the advantages of short supply period of seedlings, high uniformity of seedlings, stable genetic traits, etc. Therefore, the inventors tried to obtain excellent white wax clone plants through the method of in vitro culture of white wax stem tips and stem segments, which is a great...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁美霞张洪霞朱现威刘文娟周黛媛石宇佳王一妃李鑫竹
Owner 宁波和养生物科技有限公司
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