32 results about "Aquilaria malaccensis" patented technology

The invention relates to the field of biotechnology and discloses an agilawood production method. The method comprises the steps of first drilling a hole in a trunk of aquilaria malaccensis or aquilaria sinensis, enabling an agilawood inducer formed by plant hormones, inorganic salt and phenethyl alcohol to be added dropwise into the tree in a transfusion mode, then sealing the hole, adding the agilawood inducer dropwise at an interval of 1-2 months, performing treatment for 3-24 months in total, then cutting brown oily matter and yellowish-brown discolored xylem in the trunk, and obtaining agilawood after drying in the sun. The plant hormones are one or more than two of methyl jasmonate, ethephon and 6-BA; and the inorganic salt is one or both of sodium chloride and magnesium chloride. Components of the agilawood inducer are adjusted on the basis of an existing agilawood inducer, a trunk defense system is excited, related enzymes for synthesizing agilawood chemical components are generated and act on phenethyl alcohol, the trunk generates related chemical components in the agilawood, and finally the agilawood is formed by accumulation. According to the method, not only are contents of agilawood oil and sesquiterpenes in the agilawood oil improved, but also contents of alcohol-soluble extracts are improved.

The invention relates to a method for the rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid. The method comprises the following steps of: culturing and fermenting Fusarium proliferatum obtained by separating and purifying the agilawood generating part of the agallochum; and then, instilling the fermentation liquid into the drilled holes on the agallochum, so that the fermenting liquid induces the agallochum to generate agilawood in the agallochum. The method provided by the invention is simple, and the time for the formation of the agilawood is shortened to be 1 year; compared with the existing artificial agilawood generating method, the yield of the agilawood is increased by 4-5 times, and 200-300 grams of agilawood can be produced by a tree trunk with the length of 1 m and the diameter of 10 cm.

The invention relates to a natural Chinese Eaglewood inducer and a Chinese Eaglewood production method, and belongs to the technical field of plant cultivation. Drug effect components of the natural Chinese Eaglewood inducer are composed of a plant endogenous wounding signal, a natural Chinese Eaglewood condensation inducing molecule, a plant nutrition agent and a plant immunizing agent. The Chinese Eaglewood production method comprises the following steps: boring the trunk of an Aquilaria plant, injecting the natural Chinese Eaglewood inducer to Aquilaria malaccensis by using plant transpiration pull, sealing the obtained pore, carrying out treatment for 3-24 months, cutting the trunk, taking a dark brown oily substance and yellowish-brown allochroic xylem, and drying the dark brown oily substance and yellowish-brown allochroic xylem to form Chinese Eaglewood. The drug effect components of the natural Chinese Eaglewood inducer are safe, are natural plant components, and contain no hormones, chemical drugs or artificial perfumes; the Chinese Eaglewood inducer allows the Chinese Eaglewood to be produced in a high-efficiency, high-quality and high-yield manner, and allows standards of National Formulary 2015 to be reached after 3-month Aquilaria malaccensis condensation; and the inducer substantially improves the content of alcohol-soluble extract, Agarotetrol, volatile oil and sesquiterpene.

The invention provides a microcapsule for promoting Aquilaria sinensis trees or thymelaeaceae trees to produce agilawood. The microcapsule is wrapped with agilawood inducer, and the agilawood inducer is prepared from the following components in parts by weight: 10-30 parts of plant hormone, 50-75 parts of inorganic salt and 5-15 parts of phenethyl alcohol. The invention also provides a preparation method of the microcapsule and a method for promoting agilawood formation of the Aquilaria sinensis tree or the thymelaeaceae tree. According to the method for producing agilawood, a condition similar to the natural agilawood-producing condition can be acquired, little tree damage is caused, the trees can be subjected to agilawood formation again after agilawood collection, and the yield and the quality of the formed agilawood are effectively improved.

The present invention provides an aquilaria malaccensis tea preparation method, which comprises the following steps: sun-withering, to be more specific, sun-withering aquilaria malaccensis fresh leaves, and spreading the sun-withered aquilaria malaccensis fresh leaves to cool; rocking of the green leaves, to be more specific, rocking the aquilaria malaccensis fresh leaves spread to cool for a plurality of times; fermentation, to be more specific, fermenting the rocked aquilaria malaccensis fresh leaves; fixation, to be more specific, fixing the fermented aquilaria malaccensis fresh leaves; kneading, to be more specific, kneading the fixed aquilaria malaccensis fresh leaves until the aquilaria malaccensis fresh leaves are granular; and baking, to be more specific, baking the kneaded aquilaria malaccensis fresh leaves by charcoal baking method. Aquilaria malaccensis tea prepared by the method has elegant and fresh fragrance and delicate taste.

The invention discloses a microbody propagation expanding method of aquilaria malaccensis. The microbody propagation expanding method comprises the following steps of pre-treating an explant, inducing buds, propagating the buds and invigorating seedlings, rooting and culturing, exercising seedlings and transplanting. With the adoption of the method provided by the invention, a clonal propagation technology system of the aquilaria malaccensis is established, a clonal propagation method is provided for the precious medicinal plant of the aquilaria malaccensis, and a new way is provided for large-scale popularization of clonal seedlings.

The invention relates to the field of microorganisms and discloses a method for producing agilawood. The method comprises the following steps: drilling holes in a trunk or branches of an agilawood tree or aquilaria sinensis tree; plugging microcapsules wrapped by mixed strain such as Rhizoctonia, Fusarium oxysporum, Acremonium strictum, dense Acremonium strictum, endogenous Acremonium strictum and Penicillium into holes; then, sealing the holes; adding the microcapsules once at an interval of one to two months; after processing for 3-24 months, cutting sepia oily substances and yellowish-brown color-changing xylem parts in the trunk; and air-drying to obtain the agilawood. According to the method disclosed by the invention, the existing mixed strain for stimulating formation of the agilawood is wrapped and placed in the agilawood tree or aquilaria sinensis tree through a sodium alginate-calcium chloride system, the strain is released as well as grows and propagates in the trunk body to stimulate the trunk or the branches to generate the agilawood together, and both the content of alcohol-soluble extract and agilawood oil in the agilawood and the content of sesquiterpenes in the agilawood oil are higher than those of an existing method.

The invention discloses a method for promoting aquilaria sinensis to grow fragrance under differential stress. The method for promoting aquilaria sinensis to grow fragrance under differential stress comprises the steps of selecting, fertilizing, catalyzing fragrance by a liquid infusion method and catalyzing fragrance by peeling and liquid brushing. The fragrance growing amount of the aquilaria sinensis tree subjected to differential stress treatment in the method is 100-120% higher than that of the aquilaria sinensis tree subjected to single stress (only liquid infusion or only liquid brushing).

The invention discloses an aquilaria sinensis differential stress method for promoting fragrance formation, and belongs to the technical field of fragrance formation of aquilaria sinensis. The aquilaria sinensis differential stress method for promoting fragrance formation comprises the following steps of (1) selecting aquilaria sinensis trees; (2) performing organic fertilizer applying before fragrance formation promoting treatment; (3) performing fragrance formation promoting through an infusion method, that is, configuring a fragrance formation mother liquid, and infusing the fragrance formation liquid into the trunk; and (4) performing peeling and fragrance formation liquid brushing, that is, after the infusion of the fragrance formation liquid for promoting fragrance formation is carried out for half a year, uniformly brushing the trunk with the fragrance formation mother liquid for three times repeatedly. By adoption of the aquilaria sinensis differential stress method for promoting fragrance formation, the fragrance formation yield of the aquilaria sinensis tree is highly increased compared with that in a single stress mode (pure infusion or pure liquid brushing).

The invention discloses an agilawood semi-lignification twig cutting seedling raising method which comprises the following steps: (1) cultivating rootstocks: filling nutrient soil in a nutrient bag as a breeding matrix, and pruning when the seedling cultivation growth vigor height is 17-20cm higher than the ground; (2) preparing a mixed substrate: fully mixing peat soil, perlite and vermiculite according to a volume ratio of 1: 1: 1 to obtain the mixed substrate; (3) cutting slips: bundling every 30-50 treated cutting slips as a group, putting the cut parts at the lower ends of the bundled cutting slips into a rooting powder solution for 15-30 seconds, and then sequentially cutting the cutting slips into a substrate; (4) later-stage management and protection, wherein the cuttage environment is disinfected regularly. The method has the advantages that the method is used for raising seedlings through slope barrier cutting, the technical bottleneck that the agilawood trees are difficult to root is broken through, the agilawood trees start to root 30-35 days after cutting and can be transplanted 4-5 months later, the rooting time is effectively shortened, the cutting rooting rate and the transplanting survival rate of the agilawood trees are obviously increased, and the rooting quality of cutting slips is good.

The present invention relates to phorbol esters from the seeds of Aquilaria malaccensis by a series of chromatographic processes, and compositions containing these congeners for the treatment or prevention of allergic responses. Furthermore, compositions are provided which comprise these phorbol esters in combination with at least one to assess the effects for preventing or treating allergies.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to aquilaria malaccensis wine and a preparation method thereof. The aquilaria malaccensis wine is prepared from the following components in parts by weight: 350 to 500 parts of base wine, 24 to 30 parts of an aquilaria malaccensis extract and 56 to 70 parts of a medicinal material extract; the mass ratio of the aquilaria malaccensis extract to the medicinal material extract is 3:7; and the medicinal material extract is prepared by extracting the following medicinal materials: fructus schisandrae, fructus lycii, rhizoma dioscoreae, radix ophiopogonis and red ginseng. The aquilaria malaccensis is scientifically matched with the fructus schisandrae, the fructus lycii, the rhizoma dioscoreae, the radix ophiopogonis and the red ginseng, so that the aquilaria malaccensis wine has the effects of improving immunity and resisting fatigue. When a user enjoys the elegant fragrance of the machilus parviflora, the aquilaria malaccensis wine also has the effects of improving immunity and resisting fatigue, and is beneficial to health and health preservation.

The invention discloses an SNP molecular marker for identifying aquilaria sinensis, aquilaria yunnanensis and aquilaria malaccensis, wherein the SNP molecular marker is positioned at 81-84st basic groups of the sequences shown in SEQ ID NO. 1, SEQ ID NO. 2 or SEQ ID NO. 3; if the 81-84st basic groups are TGCC, the marker is identified as the aquilaria sinensis; if the 81-84st basic groups are CGCT, the marker is identified as the aquilaria yunnanensis; and if the 81-84st basic groups are CGCC, the marker is identified as the aquilaria malaccensis. A system for rapidly identifying the aquilariasinensis, the aquilaria yunnanensis and the aquilaria malaccensis is established based on the SNP molecular marker, and thus the purpose of quickly and accurately detecting the aquilaria sinensis, the aquilaria yunnanensis and the aquilaria malaccensis is achieved, so that the marker is of great significance for species inspection at ports and the like.

The invention relates to a method for separating bark rot pathogenic bacteria from lignum aquilarae by using a host substrate. The method comprises the following steps: (1) collecting an onset samplefrom a lignum aquilarae planting field; (2) pretreatment of a lignum aquilarae host substrate: cutting lignum aquilarae branches into pieces, soaking the cut lignum aquilarae branch pieces, and filtering off moisture; (3) test tube split-charging: loading the cut branch pieces to test tubes, and sterilizing the loaded test tubes in a high-pressure steam sterilizing pot for later use; (4) separation of stem pathogenetic fungi of lignum aquilarae: shearing tissue pieces of a diseased-healthy joint of diseased sample tissue, loading the sheared tissue pieces into the test tubes, and carrying outculture in a constant-temperature incubator; (5) purification on a potato dextrose agar culture medium: picking mycelium pieces of growth tail ends, and culturing the picked mycelium pieces on the culture medium, so as to obtain a pure culture; (6) pathogenicity assaying; and (7) pathogenic bacterium morphology observation. According to the method, by taking the host substrate as the culture medium, propagation of bacteria can be prevented, pathogenic bacterium mycelia with relatively high growth speed can exceed microbes with relatively low growth speed and are effectively separated from hybrid microbes, and thus, pure strains are obtained.

A method of producing agarwood resin includes: Step (a): forming 4 to 8 first injection holes on an agarwood tree toward a pith of the agarwood tree, wherein each of the first injection holes has a starting end and a terminal end opposite each other, the starting ends of the 4 to 8 first injection holes are evenly distributed around a tree circumference of the agarwood tree, and the terminal ends of the 4 to 8 first injection holes are connected to the xylem of agarwood tree; and Step (b): supplying a gas with a pump into the 4 to 8 first injection holes under pressure to damage the xylem of the agarwood tree in order to produce agarwood resin.

The invention relates to a natural Chinese Eaglewood inducer and a Chinese Eaglewood production method, and belongs to the technical field of plant cultivation. Drug effect components of the natural Chinese Eaglewood inducer are composed of a plant endogenous wounding signal, a natural Chinese Eaglewood condensation inducing molecule, a plant nutrition agent and a plant immunizing agent. The Chinese Eaglewood production method comprises the following steps: boring the trunk of an Aquilaria plant, injecting the natural Chinese Eaglewood inducer to Aquilaria malaccensis by using plant transpiration pull, sealing the obtained pore, carrying out treatment for 3-24 months, cutting the trunk, taking a dark brown oily substance and yellowish-brown allochroic xylem, and drying the dark brown oily substance and yellowish-brown allochroic xylem to form Chinese Eaglewood. The drug effect components of the natural Chinese Eaglewood inducer are safe, are natural plant components, and contain no hormones, chemical drugs or artificial perfumes; the Chinese Eaglewood inducer allows the Chinese Eaglewood to be produced in a high-efficiency, high-quality and high-yield manner, and allows standards of National Formulary 2015 to be reached after 3-month Aquilaria malaccensis condensation; and the inducer substantially improves the content of alcohol-soluble extract, Agarotetrol, volatile oil and sesquiterpene.

The invention discloses an SNP molecular marker for identifying aquilaria sinensis, aquilaria yunnanensis and aquilaria malaccensis and an application of the marker. A system for rapidly identifying the aquilaria sinensis, the aquilaria yunnanensis and the aquilaria malaccensis is established based on the SNP molecular marker, and thus the propose of rapidly and accurately detecting the aquilariasinensis, the aquilaria yunnanensis and the aquilaria malaccensis is realized, so that the marker is of great significance for species inspection at ports and the like.

The invention relates to the field of biotechnology and discloses an agilawood production method. The method comprises the steps of first drilling a hole in a trunk of aquilaria malaccensis or aquilaria sinensis, enabling an agilawood inducer formed by plant hormones, inorganic salt and phenethyl alcohol to be added dropwise into the tree in a transfusion mode, then sealing the hole, adding the agilawood inducer dropwise at an interval of 1-2 months, performing treatment for 3-24 months in total, then cutting brown oily matter and yellowish-brown discolored xylem in the trunk, and obtaining agilawood after drying in the sun. The plant hormones are one or more than two of methyl jasmonate, ethephon and 6-BA; and the inorganic salt is one or both of sodium chloride and magnesium chloride. Components of the agilawood inducer are adjusted on the basis of an existing agilawood inducer, a trunk defense system is excited, related enzymes for synthesizing agilawood chemical components are generated and act on phenethyl alcohol, the trunk generates related chemical components in the agilawood, and finally the agilawood is formed by accumulation. According to the method, not only are contents of agilawood oil and sesquiterpenes in the agilawood oil improved, but also contents of alcohol-soluble extracts are improved.

The invention relates to an application of an Aquilaria malaccensis seed extract in the preparation of a skin anti-inflammation composition. The extract is prepared by the following steps of: crushing a plurality of Aquilaria malaccensis seeds, performing ultrasonic extraction by using 90% ethanol, extracting each batch of crushed Aquilaria malaccensis seeds for three times, extracting once a day, removing all alcohol-water solvents by using a vacuum concentrator and a vacuum pump after the extraction is finished, and obtaining the Aquilaria malaccensis seed extract which can be applied to skin inflammation resistance.

The invention discloses a method for improving aquilaria agallocha from low-quality aquilaria sinensis. The method comprises the following steps: firstly, selecting aquilaria sinensis which are more than 5 years old and still do not produce agilawood or produce little agilawood after artificial agilawood production for improvement; cutting or sawing a notch with a flat bottom and an inclined upper part at the part, 40 cm above the ground, of the trunk of the selected to-be-improved aquilaria sinensis; cutting one to four grafting unions on a cambium between the bark at the flat bottom of the notch and the xylem of the trunk by using a knife according to the size of the trunk; at the position of the formed grafting opening, grafting the bud sticks of the seed trees of the Machilus davidii or the bud sticks of the first-generation Machilus davidii, wherein the length of the bud sticks is 5-15 cm, and the bud sticks at least comprise one leaf bud; and after grafting is completed, completely binding a grafting opening with a preservative film or a plastic film. The method for improving aquilaria agallocha from the low-quality aquilaria sinensis has the advantages that the planting time is short, and the yield and quality of the aquilaria agallocha are far better than those of agilawood trees planted in a traditional planting mode during aquilaria agallocha forming time.

The invention relates to an application of an Aquilaria malaccensis seed extract in preparing a skin repair composition. The extract is prepared by the following steps of: crushing a plurality of Aquilaria malaccensis seeds, performing ultrasonic extraction by using 90% ethanol, extracting each batch of crushed Aquilaria malaccensis seeds for three times, extracting once a day, removing all alcohol-water solvents by using a vacuum concentrator and a vacuum pump after the extraction is finished, and obtaining the Aquilaria malaccensis seed extract which can be applied to skin repair.

The invention discloses a method for inducing agilawood to produce agilawood by utilizing high-pressure gas and application of the method. The method comprises the following steps: (1) manufacturing an inflating device: drilling a hole in an aquilaria sinensis trunk, winding a thin film on the outer side of one end of a steel pipe, inserting the steel pipe into the hole, winding a thin film at a joint of the steel pipe and the trunk, coating a sealant at a gap between the steel pipe and a tree hole and the joint of the trunk for sealing, connecting a rubber pipe at the other end of the steel pipe, and sealing the rubber pipe; the rubber tube is clamped by a clamp after being folded; (2) high-pressure inflation treatment: loosening a clamp on the rubber pipe, inflating high-pressure gas into the hole in the aquilaria sinensis trunk through the rubber pipe, and performing inflation treatment once every 7-15 days for 6-12 times in total; wherein the high-pressure gas is at least one of ethylene, nitrogen and carbon dioxide. According to the method, gaseous substances are utilized, conduction and physiological and biochemical reaction of gas in a tree body are promoted in a high-pressure mode, the agilawood formation induction time is shortened, and the content of chromone, sesquiterpenoids and other substances contained in produced agilawood is higher.

The invention discloses a biophysical method for inducing agarwood to form an aroma and an application thereof. The method disclosed by the invention comprises three steps of strain preparation, groove preparation and strain inoculation. The invention utilizes the combination of burn wound surface and fungal induction to promote the activation of hyphae, increase the infection rate, and shorten the induction time of incense. The method for inducing incense formation of the present invention is low in cost, easy to operate, and strong in practicability, and is suitable for a large number of agarwood tree growers to carry out artificial agarwood formation. The content of linalool extract conforms to the national pharmacopoeia standard.

The invention discloses a method for producing agilawood by burning aquilaria trees and relates to the technical field of bioengineering. The method includes: selecting the aquilaria trees more than 10 years in age or larger than 20cm in diameter, barking the trees prior to drying in the sun, and then burning the trees from the tree bottoms to the positions 50%-60% of the three height by a flame gun, wherein peripheral portions of wounds of the aquilaria trees turn into agilawood after 6-12 months. By the method for producing the agilawood by burning the aquilaria trees, agilawood producing area can be increased, yield is 15 times higher than that of a traditional agilawood producing method, is 3 times higher than that a cauterization method and is 80% higher than those of a chemical stimulation method and a bacteria forming method, and extract of agilawood oil reaches 15%-26% and is high in purity.

The invention belongs to the technical field of agriculture and forestry, and discloses a method for promoting agilawood tree to produce agilawood by girdling bark, and the method comprises the following steps: selection and pretreatment of white agilawood tree species: carrying out root irrigation treatment by using a nutrient solution composition; girdling bark of the aquilaria sinensis trees subjected to root irrigation treatment; coating a protective glue composition on the formed exposed white wood: tearing off the whole bark upwards from the base girdling bark until the upper girdling bark is reached, and forming the exposed white wood on the whole tree; after cracks appear on the exposed white wood, smearing a fragrance promoting glue composition; about two months later, an edgeworthia chrysantha layer is formed at the wooden layer. According to the method, the bark is girdled comprehensively, so that the whole agilawood tree is damaged in the cambium area. And the tree body is protected from death due to peeling injury through the protective glue composition, and the whole plant of the cortex of the tree body is promoted to produce aroma. The method is high in agilawood producing rate, large in agilawood producing area and stable in agilawood grease quality.

A Aquilaria malaccensis seed extract for use in preparation of skin care composition is provided. Multiple Aquilaria malaccensis seeds are washed for three times and drained to dry in room temperature, and are then spread on a water-absorbing material for drying in shade. The Aquilaria malaccensis seeds so dried are pulverized to a particle size not greater than 2 mm. The pulverized Aquilaria malaccensis seeds are subject to ultrasonic extraction with 90% ethanol in such a way that the pulverized Aquilaria malaccensis seeds are subjected to extraction for three times, one time for each day. After the completion of the extraction, all ethanol-water solvent is removed to obtain Aquilaria malaccensis seed extract (Crude-EtOH).

The invention discloses an inoculation method for promoting an agilawood tree to generate fragrance, which comprises the following steps of: inputting a variety of different bacterial solutions into the xylem of the agilawood tree by utilizing infusion equipment in turn to gradually induce the agilawood tree to generate fragrance, wherein the steps are as follows: a first bacterial solution is input into the xylem of the agilawood tree to enable the agilawood tree to secrete resin along the xylem to generate fragrance; a second bacterial solution and a third bacterial solution are input to enable the bacterial solutions to mutually repel each other so as to enlarge the fragrance generating range; a fourth bacterial solution and a fifth bacterial solution are input to make each bacteria solution interact to stimulate the agilawood tree to secrete resin with different components so as to produce fragrance with different levels; a sixth bacterial solution is input to relax and stabilize the fragrance generating range of the agilawood tree. Through the above steps, the agilawood tree can continuously secrete resin in the peripheral tissues of a fragrance generating area, the fragrancegenerating range is enlarged, fragrance with different levels are produced, and the quality of the agilawood is improved.

The present invention relates to phorbol esters from the seeds of Aquilaria malaccensis by a series of chromatographic processes, and compositions containing these congeners for the treatment of allergic responses.