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Microbody propagation expanding method of aquilaria malaccensis

A kind of agarwood and micro-body technology, applied in gardening methods, botanical equipment and methods, gardening, etc.

Inactive Publication Date: 2013-01-16
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, when promoting this tree species in most areas, the traditional seedling method is still mainly used, and there are certain difficulties and obvious limitations in maintaining the characteristics of excellent varieties, providing planting materials with consistent traits, and increasing the propagation speed. However, it is still blank to study the application of tissue culture rapid propagation technology to cultivate excellent seedlings, propagate and promote excellent clonal strains.

Method used

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  • Microbody propagation expanding method of aquilaria malaccensis
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  • Microbody propagation expanding method of aquilaria malaccensis

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Malay agarwood micro-propagation method, including the following steps:

[0026] (1) Pretreatment of explants

[0027] The material is selected on a sunny day, watering is stopped one day before the material is collected, the tender stems of the healthy, disease-free agarwood seedlings are selected, the leaves are removed, brought back to the laboratory, rinsed under running water to remove obvious dirt, and then use about 0.1% Gently rinse with detergent, rinse under running water, use sterilized scissors to cut it into 1-2cm long sections with buds and stems, select 0.1% HgCl 2 Sterilize the described bud stem section for 4-5min, for subsequent use;

[0028] (2) Induction of buds

[0029] Utilize the pretreated explants as the test material, take MS, 1 / 2MS, B5 as the basic medium, add 6-BA, NAA, KT 3 kinds of plant growth regulators, wherein the medium contains sucrose 30g / L, The agar powder was 5.8g / L, and the orthogonal design method was used to screen the best s...

Embodiment 2

[0050] In step (5), a volume ratio of 1:1:1 vermiculite: perlite: loess was used as the matrix, and other steps were the same as in Example 1. A total of 125 plants were transplanted, 55 plants survived, and the transplant survival rate was 44.32%.

Embodiment 3

[0052] In step (2), the formula of induction medium is 1 / 2MS, 6-BA 0.2mg / L, NAA 0.05mg / L, sucrose 20g / L, agar 5.8g / L; other steps are the same as in Example 1, induction The startup rate is 67.5%.

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Abstract

The invention discloses a microbody propagation expanding method of aquilaria malaccensis. The microbody propagation expanding method comprises the following steps of pre-treating an explant, inducing buds, propagating the buds and invigorating seedlings, rooting and culturing, exercising seedlings and transplanting. With the adoption of the method provided by the invention, a clonal propagation technology system of the aquilaria malaccensis is established, a clonal propagation method is provided for the precious medicinal plant of the aquilaria malaccensis, and a new way is provided for large-scale popularization of clonal seedlings.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a micro-propagation method of Agarwood mellifera. Background technique [0002] There are 15 species of Aquilaria, belonging to the family Thymelaeaaceae, with precious medicinal purposes. Its form is arbor or small tree, deciduous or not, distributed in Myanmar, Thailand, Vietnam, Laos, Cambodia, India Northeast and Bhutan, Malay Peninsula, Sumatra, Kalimantan and other tropical and subtropical regions. There is one species of Aquilaria sinensis in my country, which is produced in Fujian, Guangdong, Hainan, Guangxi, Vietnam and Thailand. The agarwood tree is mainly used to produce precious medicinal agarwood. The agarwood produced with soil agarwood is a domestic agarwood. The domestic agarwood contains only 0.8% volatile oil. The main components in the oil are agarwood, agarwood, agarwood, and resin. Malay agarwood contains 1% volatile oil, and the main c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张卫华潘文朱报著许丽萍张弘
Owner GUANGDONG ACAD OF FORESTRY
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