Microbody propagation expanding method of aquilaria malaccensis
A kind of agarwood and micro-body technology, applied in gardening methods, botanical equipment and methods, gardening, etc.
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Embodiment 1
[0025] Malay agarwood micro-propagation method, including the following steps:
[0026] (1) Pretreatment of explants
[0027] The material is selected on a sunny day, watering is stopped one day before the material is collected, the tender stems of the healthy, disease-free agarwood seedlings are selected, the leaves are removed, brought back to the laboratory, rinsed under running water to remove obvious dirt, and then use about 0.1% Gently rinse with detergent, rinse under running water, use sterilized scissors to cut it into 1-2cm long sections with buds and stems, select 0.1% HgCl 2 Sterilize the described bud stem section for 4-5min, for subsequent use;
[0028] (2) Induction of buds
[0029] Utilize the pretreated explants as the test material, take MS, 1 / 2MS, B5 as the basic medium, add 6-BA, NAA, KT 3 kinds of plant growth regulators, wherein the medium contains sucrose 30g / L, The agar powder was 5.8g / L, and the orthogonal design method was used to screen the best s...
Embodiment 2
[0050] In step (5), a volume ratio of 1:1:1 vermiculite: perlite: loess was used as the matrix, and other steps were the same as in Example 1. A total of 125 plants were transplanted, 55 plants survived, and the transplant survival rate was 44.32%.
Embodiment 3
[0052] In step (2), the formula of induction medium is 1 / 2MS, 6-BA 0.2mg / L, NAA 0.05mg / L, sucrose 20g / L, agar 5.8g / L; other steps are the same as in Example 1, induction The startup rate is 67.5%.
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