Method for rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid

A technology of fermented liquid and agarwood tree, which is applied in the field of bioengineering, can solve problems such as unfavorable application and promotion, environmental conditions, etc., and achieve the effects of short aroma production cycle, simple production process, and high agarwood yield

Inactive Publication Date: 2012-07-11
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the success of the inoculation is greatly affected by the environmental conditions, and the range of forming agarwood is limited to the surrounding area of ​​the inoculation, in order to increase the yield,

Method used

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  • Method for rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid
  • Method for rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1 Application of fungal fermentation liquid to induce the method of agarwood to form agarwood rapidly

[0031] 1) Isolate and regenerate Fusarium proliferatum from the fragrant part of the agarwood tree

[0032] a) Sampling: sampling at the fragrant part of the agarwood tree;

[0033] b) Sample disinfection: soak in 0.1% mercuric chloride solution for 1 min, wash with sterile water, soak in 75% ethanol for 1 min, and wash with sterile water;

[0034] c) Separation and purification of strains: Pick the central part of the sample with sterile scissors on the ultra-clean workbench, place it in PDA medium, and cultivate it at a constant temperature of 26-28°C. After the hyphae grow, pick a single sample from the plate. Transfer the hyphae of the colony to a new plate, and continue to cultivate at a constant temperature of 26-28°C;

[0035] d) transfer the bacteria strain obtained in step c) to the slope of the test tube for cryopreservation.

[0036] 2) Cultiv...

Embodiment 2

[0043] Embodiment 2 Application method of the present invention induces the test of the output of agarwood to produce agarwood

[0044] Choose 5 strains of 6-year-old soil agarwood trees, and name them A1-A5 respectively. The average diameter at breast height of the 5 soil agarwood trees is 12.46cm. At 30cm from the ground, use an electric drill on the trunk section with a diameter of 11-15cm (drill bit Φ=0.2cm ) Drill a small hole about 3cm deep. Take 1000ml of fermented Fusarium fermented liquid and put it in an infusion set. Under the condition of fine weather, pour the fungal fermented liquid into the pre-drilled small hole into the tree, and the infusion will be completed after about 2 to 3 days. Infusion again after 2 months. After 1 year, remove the 1m trunk section (up to 70cm away from the infusion port and down to 30cm away from the infusion port), remove the non-tan part, and after drying, it becomes agarwood, measure its weight and refer to the 2010 edition of "Ch...

Embodiment 3

[0047] Embodiment 3 application method of the present invention induces the test of the output of agarwood to produce agarwood

[0048] Select 5 soil agarwood trees that are 6 years old and name them B1-B5 respectively. The average diameter at breast height of the 5 soil agarwood trees is 11.21cm, select side branches with a diameter of about 4cm, and use an electric drill with a drill bit Φ=0.2cm to drill a small hole with a depth of About 2cm. Take 1000ml of Fusarium regenerated fungal fermentation liquid and put it in an infusion set. Under the condition of fine weather, instill the fungal fermentation liquid into the pre-drilled small hole to the tree body, and the infusion is completed after about 2 to 3 days. Infusion again after 2 months. After 1 year, remove the 1m lateral branches (up to 70cm away from the infusion port and down to 30cm away from the infusion port), remove the non-tan part, and after drying, it becomes agarwood. Measure its weight and refer to the 20...

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Abstract

The invention relates to a method for the rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid. The method comprises the following steps of: culturing and fermenting Fusarium proliferatum obtained by separating and purifying the agilawood generating part of the agallochum; and then, instilling the fermentation liquid into the drilled holes on the agallochum, so that the fermenting liquid induces the agallochum to generate agilawood in the agallochum. The method provided by the invention is simple, and the time for the formation of the agilawood is shortened to be 1 year; compared with the existing artificial agilawood generating method, the yield of the agilawood is increased by 4-5 times, and 200-300 grams of agilawood can be produced by a tree trunk with the length of 1 m and the diameter of 10 cm.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for inducing agarwood to rapidly form agarwood by applying fungal fermentation liquid. Background technique [0002] Agarwood is a traditional precious medicinal material and a world-renowned precious spice in my country, Japan, India and Southeast Asian countries. The so-called agarwood is a product of some species of the genus Agarwood and some species of the genus Pseudo-agarwood in the family Daphneaceae that undergo biochemical processes through natural, microbial or artificial methods. Plants that can produce agarwood are collectively called agarwood trees. There are 18 species of Agarwood, mainly distributed in India, China, Southeast Asia and the Malay Peninsula. There are two species of Agarwood and Yunnan Agarwood in my country. [0003] There are five kinds of existing artificial fragrance-making methods. First, the method of chopping: the trunk is w...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G1/04
Inventor 马华明梁坤南周再知黄桂华林明平
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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