21results about How to "Increase total output" patented technology

The invention discloses a modified artificial'two-step 'terpene production way and high-yield rimane type terpenoids by using escherichia coli, hydrolase and drimenol synthase are subjected to fusion expression for the first time, and the yield is increased by 7.3 times. The phosphorylation efficiency is further improved by 3.6 times by combining a two-step method path PhoN with rational design, finally, the yield of drimenol is improved by 27 times by optimizing fermentation conditions and is improved to 398.4 mg / L from 15 mg / L, a high-yield system is successfully migrated to production of albicanol, and the yield of 3.5 g / L is obtained.

The invention discloses an rhFN recombinant human chemical fibronectin process of a genetic engineering technology, and particularly relates to the technical field of genetic engineering, and the rhFN recombinant human chemical fibronectin process comprises target gene acquisition, gene cloning and vector construction, host cell selection and transformation, cell culture and screening, protein expression and optimization, and protein separation and purification. According to the rhFN recombinant human chemical fibronectin process of the genetic engineering technology provided by the invention, high-quality target genes are accurately obtained through technologies such as new-generation sequencing and gene synthesis, and the gene expression efficiency and stability are enhanced by virtue of optimized vector construction and regulatory elements; protein expression ability and specificity are improved in host cell selection and transformation, efficient culture and accurate screening are realized in cell culture and screening links, total yield is increased in a protein expression optimization link, purity and efficiency are improved in a protein separation and purification link, and product quality and safety are ensured through comprehensive quality control. The requirements in the fields of medical treatment and scientific research are expected to be better met.

The invention discloses a rabies vaccine and a preparation method thereof. The rabies virus G truncated mutant protein comprises a circular RNA (Ribonucleic Acid) molecule for coding rabies virus G truncated mutant protein and a pharmaceutically acceptable carrier, wherein the amino acid sequence of the rabies virus G truncated mutant protein is as shown in SEQ ID No.13 or a degenerate sequence thereof, and the nucleotide sequence is as shown in SEQ ID No.14 or a degenerate sequence thereof; the nucleotide sequence of the circular RNA molecule is as shown in SEQ ID No. 20. At present, RNA vaccines for resisting rabies virus infection are not applied in batches, the optimized rabies virus G protein circular RNA molecules and the optimized lipid nanoparticles are used for preparing the vaccines, and the process can be industrially amplified. The animal can be induced to generate a high-level neutralizing antibody and a more lasting drug effect only by injecting a low-dose circular RNA vaccine (16 [mu] g / vaccine) once, the vaccine is good in safety and stability, and a basis is provided for industrial preparation and application of the vaccine.

The present invention provides a method for planting cauliflower on a coastal tidal flat with high salt content, and the method for planting cauliflower on a coastal tidal flat with high salt content comprises the following steps: (1) cultivating seedlings: preparing a seedling culture medium and cultivating cauliflower seedlings; (2) leveling the land: turning the soil of the field and applying compound fertilizer; (3) transplanting seedlings: transplanting the obtained cauliflower seedlings to the field and watering and irrigating; (4) field management: applying a crop salt-resistance agent to the cauliflower, and performing weeding and pest control; (5) picking the cauliflower: picking by staged harvesting, and applying a crop salt-resistance agent after each harvest. The planting process of the method for planting cauliflower on a coastal tidal flat with high salt content of the present invention is simple and convenient, the cauliflower grows fast, has a long picking cycle and high yield. The planting method provided by the present invention can fully and effectively utilize the coastal tidal flat, improve the soil of the coastal tidal flat, and improve the economic benefits of cauliflower planting.

The invention relates to the technical field of edible mushroom cultivation, in particular to a method for shortening the growth cycle of mushroom sticks by adding a certain proportion of light calcium carbonate, which comprises the following steps of: 1, preparing a basic substrate, namely selecting sawdust, wheat bran and gypsum, and uniformly mixing according to a certain proportion to obtain the basic substrate; 2, according to the addition amount of light calcium, light calcium is added according to the mass ratio of 0.3%-0.5% of the basic matrix, mechanical stirring is conducted, water is added, stirring continues to be conducted for 30-50 min, and a light calcium matrix is obtained; 3, bagging and sterilization: bagging the light calcium substrate by an automatic bagging machine with the weight of each bag being 2-3 kg, and sterilizing for 240 minutes at the temperature of 118 DEG C after bagging is completed, so as to obtain mushroom sticks; 4, cooling and inoculating; 5, culturing and puncturing; according to the method, the growth cycle of the shiitake mushrooms is shortened, the quality of the shiitake mushrooms is improved, the nutritional value is higher, and the production cost is reduced.

The present invention discloses a method for producing stropharia by graded fermentation and a method for cultivating stropharia in rotation with celery. A method for producing stropharia by graded composting fermentation comprises the following steps: 1) mixing sawdust, lime and water to form mixture A, performing primary composting fermentation to obtain primary fermentation material; 2) mixing the primary fermentation material, cottonseed hulls, rice husks, bran, lime and water to form mixture B, performing secondary composting fermentation to obtain secondary fermentation material; 3) mixing the secondary fermentation material with water to form culture medium, sowing stropharia strains on the culture medium, performing mycelium culture, and covering with soil to form a covering layer; 4) performing fruiting culture to obtain stropharia. The method of the present application significantly shortens the time from sowing to fruiting and harvesting and significantly improves the average yield through graded composting fermentation, three-layer paving and covering with soil after the mycelium crawls to the surface of the paving.

The invention provides an agricultural portulaca oleracea enzyme as well as a preparation method and application thereof, and belongs to the technical field of agricultural biological products. The method comprises the following steps: crushing fresh purslane plants, mixing the crushed purslane plants with brown sugar and tap water according to a mass ratio of 5: 1: 20, sequentially carrying out aerobic fermentation for 4-6 weeks and anaerobic fermentation for 6-8 weeks under a dark condition at 20-30 DEG C, and carrying out solid-liquid separation after the fermentation is finished, so as to obtain filtrate, namely the agricultural enzyme. The pH value of the enzyme is 3.5-4.5, the organic matter content is larger than or equal to 30 g / L, and the enzyme contains salicylic acid, indoleacetic acid, lactic acid, asparagine and rich mineral elements. The product can be used in the planting industry, is applied in an irrigation mode after being diluted, is suitable for vegetable seedling raising and green cultivation of crops such as tomatoes and pakchoi, and can effectively improve the seedling strengthening index, yield and fruit quality. According to the method, high-value resource utilization of the purslane is achieved, and good ecological and economic benefits are achieved.

The invention relates to the field of blueberry planting, in particular to a matrix potted blueberry nutrient solution and a preparation device thereof. The preparation device comprises a mixing tank, placement grooves, a telescopic stirring mechanism and a batching mechanism, a stirring rod is slidably connected to the middle of the mixing tank, stirring blades are fixedly connected to the end, located in the mixing tank, of the stirring rod at equal intervals, the placement grooves are formed in the top end of the mixing tank at equal intervals, and the number of the placement grooves is 12. Each placing groove is filled with a compound, connecting blocks are slidably connected to the interior of the mixing tank at equal intervals, through holes are formed in one sides of the connecting blocks, and the through holes are different in size. By testing different liquid fertilizer formulas, blueberry fertilizer formulas suitable for specific climate conditions in different regions are screened out; the formulas can better meet the requirements of the blueberries for nutrition in different growth stages, so that the planting adaptability of the blueberries in the southern region is improved.

The invention discloses a tussah egg incubator. The tussah egg incubator comprises an incubator body. A ventilation opening is formed in the upper portion of the incubator and provided with an electric opening and closing exhaust window and an exhaust fan, a compressor, a heater and a humidifier are arranged at the bottom of the incubator, a uniform-temperature fan is embedded in the inner wall of one side of the incubator, a water bucket is arranged outside the incubator and communicated with the water tank through a connecting pipe, and a buoyancy switch is arranged at the water outlet end of the connecting pipe. The incubator is provided with a control processor with an operation panel; and a temperature sensor and a humidity sensor are arranged in the incubator. According to the tussah incubator, acquired data are processed through the control processor, corresponding equipment is started when temperature adjustment or humidity adjustment is needed, and timing ventilation is carried out, so that the required temperature, humidity and ventilation quantity are always kept in an incubation storehouse, the incubation rate of young silkworms is increased, and the incubation efficiency of the young silkworms is improved. The hatched young silkworms are robust in body type, active and rapid in action and enhanced in autoimmunity, and the survival rate of the strong silkworms is increased.

The application discloses a kind of drinking water supply device and its control method, it relates to water supply device technical field, the control method of the drinking water supply device includes: when needing to output hot water, judge whether to satisfy first preset condition;If satisfying first preset condition, output hot water and cold water, in this process, increase the total output of hot water and / or reduce the total output of cold water;The temperature of the hot water is between the temperature of the cold water and the temperature of the hot water.This application can solve the problem that the temperature of the first cup of hot water is low when the drinking water supply device outputs again after not outputting hot water or hot water for a period of time.

The present invention relates to a fermentation process for improving the solvent production of Clostridium beijerinckii. The present invention improves the solvent production of Clostridium beijerinckii by controlling the production of byproduct glycoproteins. The specific fermentation process is that in the process of fermenting and producing solvents with Clostridium beijerinckii as a fermentation bacterium, a feed containing a nitrogen source is added to the fermentation liquid during the logarithmic growth phase of Clostridium beijerinckii, and the nitrogen source is ammonium acetate, so that the final concentration of ammonium acetate in the fermentation liquid at the end of the addition is 3.3-12.2 g / L; the fermentation produces solvents and suppresses the large-scale production of byproduct glycoproteins. The present invention adds an ammonium acetate solution during the logarithmic growth phase of Clostridium beijerinckii fermentation without controlling the pH of the fermentation liquid, so that Clostridium beijerinckii can supplement the nitrogen source during the fermentation process without causing excessive nitrogen source, can improve the utilization rate of substrate glucose, can improve the total solvent production, and can also suppress the large-scale production of byproduct glycoproteins.

The present invention relates to the field of agricultural biotechnology, and in particular to a method for improving the cellulose degradation ability of thermophilic myceliophthora and an engineered strain. The method comprises the steps of overexpressing a homologous gene encoding a lytic polysaccharide monooxygenase and a gene encoding a β-glucosidase in thermophilic myceliophthora. alp 1‑ Mtlpmo‑Mtbgl The ability of degrading microcrystalline cellulose was significantly higher than that of the starting bacteria, and the total production of cellooligosaccharides increased by 68.53%, the total production of cellooligosaccharide acid increased by 189.08%, and the glucose production increased by 254.14%; the engineered bacteria Δ alp 1‑ Mtlpmo‑Mtbgl The ability to degrade alkali-treated corn cobs was significantly higher than that of the starting bacteria, and the total yield of cello-oligosaccharides increased by 57.11%, the total yield of cello-oligosaccharide acid increased by 129.91%, and the glucose yield increased by 212.75%. The present invention has important reference and guiding significance in improving the efficiency of cellulose degrading enzymes and has important value in reducing the cost of lignocellulosic biomass conversion.

The invention provides an electrically-assisted anaerobic reactor and a method for producing methane by treating sewage. The electrically-assisted anaerobic reactor comprises an inner cylinder and an outer cylinder, the outer cylinder is sleeved outside the inner cylinder and is of a sleeve type structure, and a top cover is arranged at the top of the outer cylinder; the top cover is provided with a water inlet pipe; the inner barrel is divided into a plurality of inner barrel compartments by a plurality of radially arranged inner barrel partition plates; each inner cylinder compartment is provided with a guide plate; the space between the inner barrel and the outer barrel is divided into a plurality of outer barrel compartments through outer barrel partition plates which are arranged in an up-and-down staggered manner; an outer barrel water outlet pipe is arranged on the outer barrel; the guide plate guides water flow to be baffled up and down in each inner cylinder compartment, penetrate through each inner cylinder compartment and enter the outer cylinder from the water outlet hole of the inner cylinder; the water flow is baffled up and down between the outer cylinder compartments through the outer cylinder partition plates which are staggered up and down and flows out from the outer cylinder water outlet pipe; the inner cylinder is provided with an inner cylinder electrode, the outer cylinder is provided with an outer cylinder electrode, the inner cylinder electrode and the outer cylinder electrode are connected with a direct-current power supply, through gas circulation, the methane concentration in the biogas and the total yield of methane are increased, and the sewage energy recovery efficiency is improved.

The invention discloses a foot-and-mouth disease vaccine and a preparation method thereof. The foot-and-mouth disease virus vector comprises a circular RNA molecule for coding foot-and-mouth disease virus VP1 truncated and mutant protein and a pharmaceutically acceptable vector, wherein the amino acid sequence of the foot and mouth disease virus VP1 truncated mutant protein is shown as SEQ ID No.13 or a degenerate sequence thereof, and the nucleotide sequence is shown as SEQ ID No.14 or a degenerate sequence thereof; the nucleotide sequence of the circular RNA molecule is as shown in SEQ ID No. 17. At present, no RNA vaccine for resisting foot-and-mouth disease virus infection exists, and the circular RNA molecule for coding foot-and-mouth disease virus VP1 truncation and mutant protein and the lipid nanoparticles are used for preparing the vaccine, so that the vaccine has the advantages of safety, high efficiency, low cost and the like. A high-level virus blocking antibody can be induced and CD8 + T cell immune response can be successfully activated by injecting a low-dose circular RNA vaccine (80mu g / ml), the vaccine is good in safety and stability, and a basis is provided for large-scale vaccine preparation and application.

The invention discloses a preparation method of nano chitin, the prepared nano chitin and application of the nano chitin. The nano chitin comprises chitin nanofibers and chitin nanowhiskers. Comprising the following steps: (1) mixing chitin powder with organic acid, reacting to obtain a mixed solution I, and centrifuging the mixed solution I to obtain an upper-layer liquid I and a lower-layer solid I; (2) mixing the lower-layer solid I obtained in the step (1) with water to obtain a mixed solution II, and performing dialysis and centrifugation to obtain an upper-layer liquid II and a lower-layer solid II; (3) performing layering treatment on the supernatant liquid II obtained in the step (2) to obtain a product containing the chitin nanowhiskers and a mixed solution III; (4) carrying out high-pressure homogenization treatment on the mixed solution III obtained in the step (3) to obtain a product containing chitin nanofibers; the mass ratio of the organic acid to the chitin powder is (0.6-1.8): 1.

The invention provides a method for increasing the yield of methane by delignification through a biological method, which comprises the following steps: carrying out delignification pretreatment on bamboo powder by using pleurotus ostreatus hypha, and carrying out anaerobic fermentation on the bamboo powder pretreated by the pleurotus ostreatus hypha to generate methane. In the growth process of the pleurotus ostreatus, a large amount of lignocellulolytic enzymes such as laccase, cellulase and chitinase are secreted, the internal structure of lignin in the bamboo powder is destroyed, and part of cellulose and hemicelluloses are decomposed into small molecules; according to the bamboo powder pretreated by the pleurotus ostreatus, substrate degradation flora can be selectively gathered, a microspace ecological niche can be optimized, methanation flora can be more easily and loosely combined with the bamboo powder pretreated by fungi, and the total methane yield of anaerobic fermentation of the bamboo powder pretreated by the pleurotus ostreatus is increased by 16.2% compared with the total yield of direct fermentation of the bamboo powder.

The invention belongs to the technical field of polypeptide raw materials, and discloses an efficient polypeptide raw material production method based on a biological fermentation technology, which comprises the following steps: 1, screening and culturing microorganisms: selecting appropriate microorganisms (bacteria, yeast and fungi) as a leavening agent, the microorganisms having efficient polypeptide synthesis ability; 2, performing microbial fermentation and liquid fermentation, wherein the liquid fermentation is mainly performed in a liquid culture medium; 3, solid-phase synthesis: optimizing the environmental conditions of polypeptide production, and controlling the temperature, the pH value, the dissolved oxygen content, the nutrient substances, the stirring rate and the fermentation time; cells are broken through an ultrasonic method, the method can be carried out at a low temperature, chemical reagents do not need to be added during ultrasonic breaking, possible pollution and influence on polypeptide are avoided, and therefore the total yield of polypeptide extracted from the cells can be remarkably increased, and the polypeptide can be produced through microorganisms on the basis of a biological fermentation technology.

The invention discloses a Cordyceps militaris liquid fermentation method for increasing the yield of cordycepin, and belongs to the technical field of biological fermentation. The invention can significantly increase the yield of cordycepin in the Cordyceps militaris fermentation liquid by adding an appropriate amount of rotenone after the Cordyceps militaris is fermented for a period of time. Compared with the yield of 189.66 mg / L of traditional fermentation without adding rotenone, the yield after adding rotenone is as high as 804.69 mg / L, that is, the total yield of cordycepin is increased by 324.28%. The method has a simple process and does not need to improve the enzyme activity by means of genetic modification or the like; the production cost is low, and there is no need to continuously feed adenine and other substrates required for the synthesis of cordycepin, and the total fermentation time of cordycepin is short, and the production efficiency is significantly improved, which is suitable for industrial production.

The present invention provides an intelligent soil deep loosening and tillage optimization method and system, including: determining the physical and chemical property indicators of soil samples; calculating the correlation between different physical and chemical property indicators and crop yields, and screening out physical and chemical property indicators with a correlation less than a preset threshold; inputting the screened physical and chemical property indicators as samples into a neural network for training to obtain a crop yield prediction model; using the crop yield prediction model to predict the yield of target crops under different soil deep loosening and tillage methods to obtain a predicted value; selecting the soil deep loosening and tillage method corresponding to the maximum predicted value as the optimal tillage method. The present invention can predict the potential yield of target crops under different tillage methods by establishing a crop yield prediction model. This method allows staff to understand the effects of different strategies before actual farming, so as to select the farming method that is most likely to bring the highest yield, thereby increasing the total yield of crops.

This utility model relates to the field of organic waste treatment technology and discloses a system for simultaneously realizing the resource utilization of biogas residue and the purification of gas. The system includes a receiving hopper, an anaerobic digester, a solid-liquid separator, a material dispersing device, a drying furnace, a pyrolysis furnace, a gas storage tank, and a gas purification unit. The receiving hopper is connected to the feed end of the anaerobic digester via a conveying device. The discharge end of the anaerobic digester is connected to the feed end of the solid-liquid separator. The discharge ends of both the material dispersing device and the solid-liquid separator are connected to the feed end of the drying furnace. The discharge end of the drying furnace is connected to the feed end of the pyrolysis furnace. This utility model introduces tar-containing pyrolysis gas generated in the pyrolysis furnace into the anaerobic digester and uses biochar as an anaerobic microbial promoter to efficiently convert tar into methane within the digester. This not only completely eliminates the pollution risk of tar to the subsequent gas purification system but also significantly increases the total methane production.

The present invention provides a method for preparing high-quality fuel oil from bio-oil, which belongs to the technical field of bio-oil, and comprises the following steps: S1: preparing bio-oil, by constructing an algae-bacteria system with an oil-containing industrial microalgae and a yeast mixture for mixed culture, and extracting to obtain bio-oil; S2: preparing Ba(OH)2 / Cu composite nanocatalyst, by mixing Ba(OH)2 solution and CuSO4 solution evenly, adding multi-walled carbon nanotubes for impregnation, filtering, washing with deionized water, vacuum drying, and calcining to obtain Ba(OH)2 / Cu composite nanocatalyst; S3: preparing high-quality fuel oil, mixing the bio-oil, Ba(OH)2 / Cu composite nanocatalyst, and methanol obtained in step S1 evenly, stirring the reaction, centrifuging, evaporating, washing, and drying to obtain high-quality fuel oil. The technical scheme of the present invention achieves the purpose of improving the quality of bio-oil while achieving high conversion rate and high yield.