92 results about "Fusarium proliferatum" patented technology

The invention relates to a method for the rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid. The method comprises the following steps of: culturing and fermenting Fusarium proliferatum obtained by separating and purifying the agilawood generating part of the agallochum; and then, instilling the fermentation liquid into the drilled holes on the agallochum, so that the fermenting liquid induces the agallochum to generate agilawood in the agallochum. The method provided by the invention is simple, and the time for the formation of the agilawood is shortened to be 1 year; compared with the existing artificial agilawood generating method, the yield of the agilawood is increased by 4-5 times, and 200-300 grams of agilawood can be produced by a tree trunk with the length of 1 m and the diameter of 10 cm.

The invention discloses bacillus subtils 203 for high effectively preventing banana wilt, and the preservation number of the bacillus subtils 203 is CCTCCM207094. The invention simultaneously discloses a process for preparation and usage. The bacillus subtils 203 are obtained after being selected by being cultured face to face on a plate and doing pot experiment, which which is capable of effectively preventing the growth of fusarium oxysporum and has wide antimicrobial spectrum. The invention is also capable of performing antagonistic action on various pathogenic fungi, such as cotton-wilt fusarium, verticillium wilt pathogen, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, curvulavia lunata or colletotrichum musae and the like, which is good in environmental security and good in prospect of development and application.

The present invention relates to the use of primers in polymerase chain reaction assays for the detection of a Fusarium proliferatum, F. verticillioides and F. subglutinans. Specific primers are identified as being useful for the identification of fungal isolates using PCR based techniques.

The invention provides bacillus velezensis for inhibiting virus and promoting plant growth and application of a bacillus velezensis SNB55 strain in prevention and treatment of plant virus diseases andplant fungal diseases and promotion of plant growth. The bacillus velezensis for inhibiting virus and promoting plant growth and the application of the bacillus velezensis have the beneficial effectsthat the bacillus velezensis has no toxicity and pathogenicity, is safe to human and livestock, is environment-friendly, and has high stress resistance and stable antibacterial activity; the bacillusvelezensis can inhibit phytopathogens such as bacillus cereus, tobacco mosaic virus, tomato yellow leaf curl virus, fusarium wilt of cucumbers, tobacco target spot pathogens, phytophthora capsici, rice sheath blight disease and botrytis cinerea, can be secrete indoleacetic acid to promote seed germination and plant growth; and the strain is a multifunctional biocontrol bacterium and has a wide application prospect.

The invention relates to bacillus amyloliquefaciens, the preservation number of which is CCTCC M 2015299, and which is named as bacillus amyloliquefaciens XK-2. The invention further provides a screening method of the bacillus amyloliquefaciens XK-2 and an application of the bacillus amyloliquefaciens XK-2 in inhibiting watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria as well as a bacillus amyloliquefaciens XK-2 bactericide. The bacillus amyloliquefaciens XK-2 is a watermelon endophytic bacterial which is safe to the human beings, animals and crops, environment-friendly, relatively wide in antibacterial spectrum, has a good inhibition effect on common soil-borne pathogen,watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria, has a significant control effect on watermelon fusarium wilt, has an obvious inhibition effect on the growth of watermelon fusarium oxysporum hypha, slows down the growth of hypha, facilitates the distortion syndrome of the hypha and is wide in application prospect in biological control of plant diseases.

The invention belongs to the field of agricultural microbiology and discloses a fusarium proliferatum strain Br-1 with the preserved number of CGMCC No. 3759. The invention also discloses a microbiological bacterium agent prepared by using the Br-1. The fusarium proliferatum strain Br-1 and the bacterium agent thereof have good inhibiting effect for orobanche cumana wallr, and the control effect in fields reaches 61.7-82.0%; and secondarily, a preparation method of the microbiological bacterium agent is simple, and has low cost and easy promotion and application. In addition, the invention belongs to a biological control method, has no pollution, no nuisance and low residue and is environment-friendly.

The invention discloses a multiple intermolecular rapid detection method for banana wilt bacteria and bacterial Erwinia carotovora. The invention provides a group of specific primers, No.1 and No.4 physiological races of the banana wilt bacteria, and bacterial Erwinia carotovora can be simultaneously detected in a one-step PCR (Polymerase Chain Reaction) amplified reaction. When a 636bp stripe presents in the PCR product, the sample contains FOC1; when a 796bp stripe presents in the PCR product, the sample contains FOC4; when a 218bp stripe presents in the PCR product, the sample contains the bacterial Erwinia carotovora. The method is easy to operate, low in time consumption and high in detection sensitivity. The sensitivity of detecting the banana wilt bacteria mycelium can be 1 mu g, and the sensitivity of detecting the bacterial Erwinia carotovora can be 10<3>CFU / ml. The method can be applied to detection of banana disease tissues, pathogenic bacteria which are directly separated or soil and has the advantages of high accuracy and the like, diffusion and spread of the banana wilt disease and bacterial Erwinia carotovora can be avoided, and safety production of bananas is guaranteed.

The invention relates to the technical field of biology, and particularly provides a Bacillus amyloliquefaciens strain named Bacillus amyloliquefaciens GKT04 (CCTCC NO: M2016784), a screening method of the Bacillus amyloliquefaciens GKT04, application of the Bacillus amyloliquefaciens GKT04 in inhibiting Banana vascular wilt, and a Bacillus amyloliquefaciens GKT04 microbial inoculant. The microbial inoculant has favorable preventing and treating effects on Banana vascular wilt, has long-time preventing actions, and is environment-friendly. Compared with other soil biocontrol bacteria, the strain can be stably colonized in the banana plant, is slightly influenced by the external environment, and can directly interact with pathogenic bacteria, thereby providing comprehensive and effective protection for plants. The experimental investigation detects that the strain can obviously promote growth of the banana plants.

The invention discloses fusarium proliferatum and the method used to manufacture ginsenoside Rh2. It belongs to fusarium proliferatum ECU2042 CGMCC No.1495. The invention catalyzes and hydrolyzes relative common ginsenoside to manufacture rare saponin, and use the fusarium proliferatum to manufacture ginsenoside Rh2. It cost is relative low. And transformation ratio can reach above 60%. The formed saponin purity is not less than 95%.

The invention provides crop growth promoting antagonistic trichoderma, in particular to Trichoderma atroviride H18-1-1 and TF28 with preservation numbers of CGMCC No.9774 and 9775 and aims to solve the problem that using agricultural chemicals for preventing and treating agricultural diseases is prone to causing environment pollution and agricultural chemical residues, jeopardizing an agricultural ecological system, killing beneficial microorganisms in the environment, improving drug resistance of phytopathogen and finally causing decline of prevention and treatment effects and even failure in prevention and treatment. The trichoderma not only can be applied to agricultural biological prevention and treatment but also can promote growth of crops such as wheat and prevent and treat diseases caused by rhizoctonia cerealis, fusarium culmorum, fusarium proliferatum and / or fusarium oxysporum and the like.

Provided are: an agent for preventing bacterial wilt disease, which agent can prevent the development of bacterial wilt disease caused by various different bacterial wilt disease strains, that is, the development of bacterial wilt disease in various different plant varieties; and a method for preventing bacterial wilt disease utilizing the agent for preventing bacterial wilt disease. The agent for preventing bacterial wilt disease comprises as an active ingredient a wilt bacterium (Ralstonia solanacearum) infected with the φRSM1-type filamentous phage and / or the φRSM3-type filamentous phage. The method for preventing bacterial wilt disease comprises the step of inoculating the agent for preventing bacterial wilt disease to a plant. The plant is preferably any one of tomato, potato, green pepper, eggplant, tobacco, capsicum, Japanese basil, Japanese radish, strawberry, banana, marguerite, chrysanthemum and sunflower.

The invention belongs to the biological technical field and particularly relates to a screening method of a high-yield diosgenin strain and an application of the strain in turmeric saponin transformation. With the adoption of the screening method, a Fusarium Proliferatum WX12 of high-yield diosgenin is screened; the strain is preserved in General Microbial Center of China Microbial Culture Collection Management Committee; the preservation number is CGMCCN0. 5901; the strain has characteristics of fast growth speed and high transformation efficiency, is applied in industrially producing the diosgenin, has the advantages of moderate reaction condition, high yield, low production cost and environment friendliness, and is convenient and quick in post treatment because a large number of acid or alkali is not needed.

The invention belongs to the field of detection of disease resistance of horticultural plants, and relates to a seedling stage identification method for cowpea wilt resistance. The method includes the steps of sprouting cowpea seeds, sowing, cultivating seedlings, separating and cultivating bacterial strains special for inoculation, preparing oxysporum spore suspension liquid, infecting pathogenic bacteria, surveying plant disease causes and the like. Cowpea seedlings are infected with oxysporum artificially with the method that seedling roots are infected with bacterium liquid, cowpea seeds are cultivated in seedling cultivating matrixes to grow two leaves and one bud, then the seedlings are pulled out from infection, the growth vigour and the size of the cowpea seedlings can be controlled easily, the cowpea seedlings can be pulled out easily, and consistency and reliability of identification results are guaranteed. The special indication bacterial strains, the appropriate infection time and the appropriate oxysporum spore suspension liquid concentration are adopted, the infection effect is good, and even inoculation and quick disease generation are achieved. By means of the method, the oxysporum infection only needs to be conducted on different plants with the consistent growth vigour after roots are damaged directly through natural friction, the method is easy and convenient to operate, quick in identification, reliable in result and particularly suitable for identifying the oxysporum disease resistance of cowpea cultivating materials in batches.

The invention discloses a biocontrol bacterium and azoxystrobin composition belonging to the technical field of bactericides. The biocontrol bacterium and azoxystrobin composition contains active components including bacillus subtilis and azoxystrobin. The biocontrol bacterium and azoxystrobin composition disclosed by the invention can be used for remarkably reducing the medication cost while effectively preventing and controlling various diseases such as rice sheath blight disease, rice false smut, rice blast, ear rot, seedling-stage damping-off, tomato late blight, gray mold, wheat powdery mildew, sheath blight, cotton rhizoctoniosis, foxysporum, verticillium dahliae, soybean root rot, corn sheath blight, cucumber gray mold, blight, tobacco brown spot, bacterial wilt, various field crop and garden crop root rots and damping off, so that the medication cost can be reduced, the preventing and controlling ranges can be widened, the environment pollution can be reduced, and the phytotoxicity and pesticide resistance risks can be reduced.

The invention provides a method for improving output of an anticancer anthraquinone compound 1403C generated by Fusarium proliferatum (No.1403) via utilizing different vaccination ways. The method comprises the following steps: a conical flask with a baffle is adopted to cultivate seeds for grinding inoculation, the seeds cultivated by the conical flask with the baffle are directly inoculated, and seeds cultivated by glass beads are added into the conical flask, therefore, the 1403C yield obtained by fermentation is improved to different degrees compared with the yield obtained by fermentation for cultivating seeds (control group) in a traditional inoculation way, wherein the maximum 1403C yield is 1.13g / L, which is improved by 289.7% compared with 0.29g / L yield of the control group. The invention has vital significance for further performing 1403C anti-cancer pharmacological effect experiments.

The invention relates to laevorotation lactone specificity hydrolytic enzyme producing strain and the method used it to make chirality oxyacid. The enzyme producing is Fusarium proliferatum Nirenberg ECU2002 with storage number CGMCC 1494. The chirality oxyacid preparing method includes the following steps: using the fungous mycelium, rough enzyme extract or their immobilization derivative as biocatalyst; processing antipode selectivity hydrolysis resolution for a series of racemic chirality lactone to gain many optically active (+)-oxyacid and (+)-lactone which can be hydrolyzed into (-)-oxyacid; (+)-alpha-hydroxyl-beta, beta-dimethyl-gamma-butyric acid which are D-(+)-pantoic acid; simple acidizing to gain chirality intermediate D-(-)-pantoic acid lactone widely used in preparing feed and daily chemical engineering industry.

The invention relates to a method for controlling melanophila picta pallas larvae through fusarium proliferatum carried on sclerodermus pupariae. In the method, the fusarium proliferatum carried on the sclerodermus pupariae is utilized to actively infect the melanophila picta pallas larvae, and the probability of infected entomogenous fungi of the melanophila picta pallas larvae is increased when parasitic sclerodermus pupariae exists; and cooperative composition effect of the sclerodermus pupariae and entomogenous pathogenic fungi has synergism effect on controlling the melanophila picta pallas larvae, so that death rate of the melanophila picta pallas larvae is increased, high selectivity and specificity for hosts are realized, nonhazardous effects on high flora and fauna are generated, environment is protected from being polluted, and a sustainable control function is provided. The method is reasonable and high in operability; the problems like large difficulties and bad effects in controlling of the melanophila picta pallas larvae as well as pesticide residues are solved; and a new way to control the melanophila picta pallas larvae is provided.

The invention provides paenibacillus polymyxa, application, and a microbial agent, a powder and a granule. The P. polymyxa is separated from corn stem, and has the advantages that the strain has goodpreventing and control effects on various types of plant fungus diseases, and especially on fungus diseases of fusarium graminearum, fusarium proliferatum or fusarium verticillioides, the pollution toenvironment is avoided, the ecological and safe effects are realized, the genetic property is stable, and the easiness in production of resistance is avoided; the P. polymyxa can be mixed with organic and inorganic nutritional components required by the plant growth to prepare the microbial agent for irrigating roots, the powder for mixing with seeds, and the granule, so that the anti-fungus disease ability of the crop is obviously enhanced.

The invention provides a primer for multi-PCR (Polymerase Chain Reaction) detection aiming at fusaria and an application of the primer. The primer is formed by three groups of primer pairs, and can be used for rapidly and accurately identifying three types of fusaria comprising fusarium graminearum, fusarium verticillioides and fusarium proliferatum resulted in maize kernel rots through a multi-PCR amplification system by one step. The detection primer has the good specificity, namely the primer only can be used for detecting stripes with specific sizes in an amplifying manner from the target fusaria but cannot be used for detecting the stripes from proximal species or other pathogenic fungi; only the time of 2 hours is consumed in the whole PCR and electrophoresis process. Thus, the primer provided by the invention is used for rapidly, simply, conveniently and accurately distinguishing the three various types of fusaria resulted in the maize kernel rots.

The invention discloses a hydroxyquinoline compound as well as a preparation method and application thereof in preventing and treating agricultural diseases. Testing results show that the compound hasremarkable activity upon corn leaf fulvia fulvum, cotton oxysporum, potato rhizoctonia solani kuhn, didymella bryoniae, fusarium oxysporum, wheat gibberella, rice blast, botrytis cinerea, lycium barbarum anthracnose, cotton anthracnose, sclerotinia sclerotiorum, pepper phytophthora blight, potato late blight, rhizoctonia solani, melon bacterial fruit plaque bacteria, grape meloidogynosis bacteria, Chinese cabbage erwinia carotovora, kiwi fruit anabrosis bacteria, pseudomonas lachrymans, ralstorinia solanacearum, rice bacterial leaf blight and rice bacterial stripe bacteria. The hydroxyquinoline compound is simple in preparation process, cheap and easy in raw material obtaining, high in product purity, good in bioactivity and wide in sterilization spectrum and has the potential of being developed as new fungicides.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer of fusarium proliferatum and application of the LAMP detection primer. The detection primer is a composition consisting of a pair of outer primers F3 / B3, a pair of inner primers FIP / BIP and a pair of loop primers Loop F / Loop B. The LAMP detection primer composition is used for detecting the fusarium proliferatum and detecting whether maize crops are infected with maize kernel rot or not. The designed LAMP detection primer composition of the fusarium proliferatum is high in specificity and good in amplification effect; a detection method has no need of performing separation, purification and culture on pathogenic bacteria, is simple, convenient and fast and is high in practicability, high in sensitivity and accurate and reliable in result; and the LAMP detection primer can be used for early diagnosis of the pathogenic primary stage and early stage of the fusarium proliferatum in maize plant tissues.

The invention provides a method for improving output of an anticancer anthraquinone compound 1403C generated by Fusarium proliferatum No.1403. The 1403C fermentation output of the Fusarium proliferatum No.1403 is improved with different degrees by adding malonic acid or mixture of sodium acetate and malonic acid, wherein the maximum improvement is obtained by adding the sodium acetate and the malonic acid in a ratio of 1: 4 and with a final concentration of 6mM, and the maximum yield of 1403C on thalli is 0.53g / g and is improved by 102.7 percent compared with 0.26g / g of the output of a control group. The method has significance for further performing anticancer pharmacological effect experiments of the 1403C.

The invention relates to a streptomyces mediolani ZW-1 bacterial strain, and a bacteriostatic application of fermentation broth thereof. Inhibited bacteria comprise wheat fusarium nivale, wheat fusarium graminearum, alternaria leaf spot, sclerotinia sclerotiorum, spinach altemaria solania, helminthosporium carbonum, watermelon fusarium wilt or cotton fusarium wilt. The streptomyces mediolani ZW-1 bacterial strain and the fermentation broth thereof can inhibit the growth of plant pathogenic fungal hyphae, lead to enlargement of cell walls of the pathogenic fungal hyphae and concentration of protoplasts, can inhibit conidial germination and germ tube growth of the spinach altemaria solania, have good bacteriostatic effects, and have good development and application prospects for biological prevention and control of plant diseases.

The invention provides trichoderma asperellum TZ312, application thereof, a volatile metabolite thereof and application of the volatile metabolite, and relates to the technical field of microorganisms. The trichoderma asperellum TZ312 is preserved in Guangdong Microbiological Culture Collection Center and has a preservation number of GDMCC NO:60253. The trichoderma asperellum TZ312 is capable of completely inhibiting and covering growth of hyphae of banana wilt disease germs on a panel; and the trichoderma asperellum TZ312 is capable of obviously decreasing the occurrence rate of a banana wiltdisease in a greenhouse pot experiment, and the prevention effect is 65% or above. The trichoderma asperellum TZ312 is further capable of inhibiting fusarium oxysporum f.sp.cucumerinum, botrytis cinerea, botrytis cinerea, fusarium solani, fusarium proliferatum, magnaporthe oryzae, phomopsis sp., pestalotiopsis sp., botryosphaeria dothidea or colletotrichum gloeosporioides and is wide in antibacterial spectrum. The volatile metabolite of the trichoderma asperellum TZ312 can be used for inhibiting preservation diseases.

The invention discloses bacillus licheniformis 202 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 202 is CCTCCM207093. The invention simultaneously discloses a process for preparation and usage. The bacillus licheniformis 202 is obtained after being selected by being cultured face to face on a plate and doing pot experiment, which is capable of effectively preventing the growth of fusarium oxysporum and has wide antimicrobial spectrum. The invention is also capable of performing antagonistic action on various pathogenic fungi, such as cotton-wilt fusarium, verticillium wilt pathogen, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, curvulavia lunata or colletotrichum musae and the like, which is good in environmental security and good in prospect of development and application.

The invention discloses a loquat endophytic unciform trichoderma strain and applications of the loquat endophytic unciform trichoderma strain. The endophytic trichoderma strain is obtained through separation, then morphological and molecular biological identification is carried out, meanwhile, the antibacterial activity of the endophytic trichoderma strain is tested, and thus a material is provided for the development and utilization of the strain; the endophytic trichoderma strain has the antibacterial activity for five phytopathogens, wherein the inhibiting effect for fungi is obviously stronger than that for bacteria, specifically, the inhibiting effect for pyricularia grisea is the strongest and is 83.17%, the antibacterial rate for alternaria tenuissima takes the second place and is 75.10%, the antibacterial rates for fusarium proliferatum and fusarium sambucinum have no obvious differences and are respectively 52.02% and 49.07%, and the antibacterial rate for Xanthomonas oryzae pv.oyzae is the poorest, and is 38.24%. The trichoderma strain has the antibacterial wide spectrum, and has the development and utilization values and prospects.

The invention discloses a ginkgo endophytic fungus and an application thereof. The ginkgo endophytic fungus fusarium proliferatum DZHQ1 with anti-tumor activity is separated from ginkgo bark, the species of a strain is judged with a colony morphology and 18sRNA sequencing combination method, the anti-cervical cancer activity of a crude extract of the strain is detected with MTT assay, and finally,secondary metabolites of the strain with inhibition rate being higher than 50% are separated with a semi-preparative HPLC method. Accordingly, compound monomers with anti-tumor activity can be further screened.

The invention discloses an endophytic fungus strain, i.e., Fusarium proliferatum G11-7, originated from a pigeon pea and capable of efficiently converting ginsenoside Rb1 into ginsenoside Rd and a separation method thereof, and application of the strain to fermentation conversion of ginsenoside. The strain G11-7 is originated from a pigeon pea plant and prepared through solid culture, separation and purification. The strain can efficiently and specifically convert ginsenoside Rb1 into ginsenoside Rd, and the content of ginsenoside Rd after conversion is about 4 times the content of ginsenoside Rd before conversion. The Fusarium proliferatum G11-7 provided by the invention is an endophytic fungus acquired from the pigeon pea for the first time. When the Fusarium proliferatum G11-7 is applied to conversion of ginsenoside Rb1 into ginsenoside Rd, conversion time is short, conversion rate is high, no pollution is produced, industrial production can be easily realized, and good application prospects are obtained.

The invention discloses a polyketone compound. The chemical structure formula of the polyketone compound is shown in the specification. The invention also discloses a preparation method of the polyketone compound. The preparation method comprises the steps of performing fermentation culture on fusarium ZS07 (Fusarium proliferatum ZS07) with a preservation number of CCTCC NO:M2013257; filtering the obtained fermentation solution, extracting the filtered solution by using ethyl acetate, concentrating in vacuum and drying to obtain a crude extract; performing silica gel column chromatography fragmentation on the crude extract, and performing gradient elution by using dichloromethane / methyl alcohol; and concentrating an elution part F2 and then recrystallizing to obtain a polyketone compound. The polyketone compound can be used as an herbicide and in particular can be used for suppressing growth of amaranthus retroflexus radicles.