Polyketone compound, preparation method and application thereof

A technology of polyketone compounds and uses, applied in the biological field, can solve problems such as no herbicidal activity, etc.

Inactive Publication Date: 2014-03-19
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A kind of existing compound, its structural formula is shown in formula 1; In document Ma S M, Zhan J

Method used

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  • Polyketone compound, preparation method and application thereof
  • Polyketone compound, preparation method and application thereof
  • Polyketone compound, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, bacterial strain screening:

[0034] In November 2012, healthy katydid samples were captured from the suburbs of Jinhua, and the samples were brought back to the laboratory for processing as soon as possible.

[0035] The medium is MEA medium: 20g of malt extract, 20g of sucrose, 20g of agar, 1g of peptone, distilled water to 1L, pH7.0. 1.1 atmospheric pressure, sterilized at 121°C for 20min (for conventional sterilization).

[0036] Methods: Healthy katydid insects were starved for 24 hours and then sterilized with 75% (volume %) alcohol for 2 minutes under aseptic conditions, rinsed with sterile water for 3 times, and then dissected. Suspension, respectively diluted with sterile water for 10 -1 、10 -2 、10 -3 times, take 0.2 mL of each gradient dilution and spread it on the MEA medium plate, and culture it in a 28°C incubator for 2-3 days. After the colony grows, pick a small amount of mycelium and transfer it to a new MEA medium plate to purify it to...

Embodiment 2

[0037] Embodiment 2, bacterial strain identification

[0038] ITS rDNA analysis:

[0039] According to the instructions of BioTeke's new rapid plant genomic DNA extraction kit, the genomic DNA of the symbiotic bacteria ZS07 obtained in the above-mentioned Example 1 was extracted, using ITS universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3'forward) and ITS4 (5'-TCCTCCGCTTATTGATATGC-3 ', reverse) to amplify the rDNA ITS region gene sequence and purify it.

[0040] The similarity of the sequencing results was compared with the BLAST program; the analysis results showed that the symbiotic fungus ZS07 had a high similarity with the strain F.Proliferatum (FJ648201), with a similarity of 99.5%. Combined with the morphological characteristics of the bacterium, the bacterium was identified as F. Proliferatum.

[0041] The bacterium has been preserved, the preservation unit: China Center for Type Culture Collection; the preservation name is: Fusarium proliferatum ZS07 (Fusarium prolife...

Embodiment 3

[0042] Embodiment 3, the preparation of polyketide compound

[0043] The mycelium block (0.5×0.5cm) of fresh Fusarium proliferatum ZS07 2 , about 0.5g) were inoculated into 250mL Erlenmeyer flasks containing 150mL ME medium in each bottle, inoculated into 5-6 bottles, and cultured at 180rpm and 28°C for 2-3 days as the seed solution.

[0044] Remarks: Fresh Fusarium ZS07 can be obtained after activation of Fusarium ZS07 by conventional malt solid medium (MEA medium).

[0045] 10 mL of seed solution was inoculated into a 1000 mL Erlenmeyer flask containing 400 mL of ME medium, and fermented for 7 days at 180 rpm and 28°C.

[0046]The fermentation broth was filtered with two layers of gauze, the filtrate was extracted with an equal volume of ethyl acetate, the number of extractions was 3 times, concentrated and dried in vacuo (at a vacuum degree of 0.1 negative pressure, 45°C for 30-50 minutes), and a crude extract 40.5 g.

[0047] The above ME medium is: 20g of malt extract,...

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Abstract

The invention discloses a polyketone compound. The chemical structure formula of the polyketone compound is shown in the specification. The invention also discloses a preparation method of the polyketone compound. The preparation method comprises the steps of performing fermentation culture on fusarium ZS07 (Fusarium proliferatum ZS07) with a preservation number of CCTCC NO:M2013257; filtering the obtained fermentation solution, extracting the filtered solution by using ethyl acetate, concentrating in vacuum and drying to obtain a crude extract; performing silica gel column chromatography fragmentation on the crude extract, and performing gradient elution by using dichloromethane/methyl alcohol; and concentrating an elution part F2 and then recrystallizing to obtain a polyketone compound. The polyketone compound can be used as an herbicide and in particular can be used for suppressing growth of amaranthus retroflexus radicles.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the preparation and herbicide application of katydid intestinal symbiotic bacterial strains and new active metabolites thereof. Background technique [0002] Amaranth (Amaranthus retroflexus L) is a typical vicious invasive weed in my country, which seriously damages dryland crops and affects crop yield. Chemical herbicides used to be the main measures to control the harm of Amaranth retroflexus, but the long-term use of chemical herbicides has caused many negative effects such as environmental pollution and ecological imbalance. In the context of human beings' thirst for safe, effective, and pollution-free new herbicides, the research on efficient and environmentally friendly microbial herbicides has attracted more and more attention from experts and scholars around the world. [0003] Insect symbionts are a class of special environmental microorganisms that are less studied. Comp...

Claims

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Application Information

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IPC IPC(8): C07D311/76C12P17/06A01P13/00C12N1/14C12R1/77
CPCA01N43/16C07D311/76C12N1/14C12P17/06
Inventor 张应烙李帅
Owner ZHEJIANG NORMAL UNIVERSITY
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