Bacillus amyloliquefaciens strain and application thereof
A technology of amylolytic bacillus and bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of difficult long-term colonization and survival, and unsatisfactory disease prevention effect, and achieve significant control effect, good control effect, and short production cycle Effect
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Embodiment 1
[0045] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) GKT04, CCTCC NO: M2016784; preservation place: China, Wuhan, Wuhan University; China Center for Type Culture Collection, preservation time is December 26, 2016.
[0046] 1 Materials and methods
[0047] 1.1 Materials
[0048] 1.1.1 Plant material
[0049] Banana, variety: Guijiao No. 1, the main banana variety in Guangxi.
[0050] 1.1.2 Pathogens tested
[0051] Fusarium oxysporumf.sp.cubense race 4 (FOC4) was isolated and screened by our laboratory.
[0052] 1.1.3 Test medium
[0053] LA medium: tryptone 10g, yeast extract 5g, NaCl 10g, agar powder 12.5g
[0054] LB medium: tryptone 10g, yeast extract 5g, NaCl 10g
[0055] NA medium: beef extract 3g, peptone 10g, NaCl 5g, glucose 4g, agar powder 12.5g
[0056] PDA culture gold: 200g of peeled potato pieces, cut into small pieces with a knife, add 1L of water to boil for 20min, filter with 3-4 layers of gauze, take the filtrate, add 20g of glucose, 18g of ag...
Embodiment 2
[0097] A microbial preparation, which is prepared by the following method:
[0098] (1) Activation: Bacillus amyloliquefaciens GKT04 was inoculated in LA medium and cultured at 28°C for 20 hours;
[0099] Among them, the formula of LA medium is: tryptone 10g, yeast extract 5g, NaCl 10g, agar powder 12.5g, pH7.0, sterilized at 121°C for 20min;
[0100] The formula of NA medium is: beef extract 3g, peptone 10g, NaCl 5g, glucose 4g, agar powder 12.5g, pH7.0, sterilized at 121℃ for 20min;
[0101] (2) Seed culture: Inoculate the activated Bacillus amyloliquefaciens GKT04 strain into NA liquid medium, vibrate at 29°C and 170r / min, and cultivate for 4h to obtain a seed culture solution;
[0102] (3) Fermentation: Inoculate the seed culture liquid with 5% inoculation ratio in the mixture containing starch 15g / L, soybean meal powder 16g / L, glucose 7g / L, yeast extract 1.5g / L, L-sodium glutamate 5g Fermentation medium made of / L, sodium nitrate 1.5g / L, dipotassium hydrogen phosphate 1...
Embodiment 3
[0107] A microbial preparation, which is prepared by the following method:
[0108] (1) Activation: Bacillus amyloliquefaciens GKT04 was inoculated in NA medium, and cultured at 29°C for 23 hours;
[0109] Among them, the formula of LA medium is: tryptone 10g, yeast extract 5g, NaCl 10g, agar powder 12.5g, pH 7.0, sterilized at 121°C for 20min;
[0110] The formula of NA medium is: beef extract 3g, peptone 10g, NaCl 5g, glucose 4g, agar powder 12.5g, pH7.0, sterilized at 121℃ for 20min;
[0111] (2) Seed culture: inoculate the activated Bacillus amyloliquefaciens GKT04 strain into NA liquid medium, vibrate at 28°C and 150r / min, and cultivate for 8 hours to obtain a seed culture solution;
[0112] (3) Fermentation: Inoculate the seed culture liquid with 5% inoculation ratio in a mixture containing starch 10g / L, soybean meal powder 15g / L, glucose 5g / L, yeast extract 1g / L, L-sodium glutamate 3g / L L, sodium nitrate 1g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate 1g / ...
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