Bacillus amyloliquefaciens strain and application thereof

A technology of amylolytic bacillus and bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of difficult long-term colonization and survival, and unsatisfactory disease prevention effect, and achieve significant control effect, good control effect, and short production cycle Effect

Active Publication Date: 2017-05-17
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For plant disease biocontrol bacteria, most of them are isolated from plant rhizosphere soil, but because soil microorganisms do not have an advantage in the competition with soil habitant microorganisms, and are not easy to colonize and survive for a long time, the actual disease prevention The effect is not ideal
There is no report on using endogenous Bacillus amyloliquefaciens to control fungal diseases of banana

Method used

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  • Bacillus amyloliquefaciens strain and application thereof
  • Bacillus amyloliquefaciens strain and application thereof
  • Bacillus amyloliquefaciens strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) GKT04, CCTCC NO: M2016784; preservation place: China, Wuhan, Wuhan University; China Center for Type Culture Collection, preservation time is December 26, 2016.

[0046] 1 Materials and methods

[0047] 1.1 Materials

[0048] 1.1.1 Plant material

[0049] Banana, variety: Guijiao No. 1, the main banana variety in Guangxi.

[0050] 1.1.2 Pathogens tested

[0051] Fusarium oxysporumf.sp.cubense race 4 (FOC4) was isolated and screened by our laboratory.

[0052] 1.1.3 Test medium

[0053] LA medium: tryptone 10g, yeast extract 5g, NaCl 10g, agar powder 12.5g

[0054] LB medium: tryptone 10g, yeast extract 5g, NaCl 10g

[0055] NA medium: beef extract 3g, peptone 10g, NaCl 5g, glucose 4g, agar powder 12.5g

[0056] PDA culture gold: 200g of peeled potato pieces, cut into small pieces with a knife, add 1L of water to boil for 20min, filter with 3-4 layers of gauze, take the filtrate, add 20g of glucose, 18g of ag...

Embodiment 2

[0097] A microbial preparation, which is prepared by the following method:

[0098] (1) Activation: Bacillus amyloliquefaciens GKT04 was inoculated in LA medium and cultured at 28°C for 20 hours;

[0099] Among them, the formula of LA medium is: tryptone 10g, yeast extract 5g, NaCl 10g, agar powder 12.5g, pH7.0, sterilized at 121°C for 20min;

[0100] The formula of NA medium is: beef extract 3g, peptone 10g, NaCl 5g, glucose 4g, agar powder 12.5g, pH7.0, sterilized at 121℃ for 20min;

[0101] (2) Seed culture: Inoculate the activated Bacillus amyloliquefaciens GKT04 strain into NA liquid medium, vibrate at 29°C and 170r / min, and cultivate for 4h to obtain a seed culture solution;

[0102] (3) Fermentation: Inoculate the seed culture liquid with 5% inoculation ratio in the mixture containing starch 15g / L, soybean meal powder 16g / L, glucose 7g / L, yeast extract 1.5g / L, L-sodium glutamate 5g Fermentation medium made of / L, sodium nitrate 1.5g / L, dipotassium hydrogen phosphate 1...

Embodiment 3

[0107] A microbial preparation, which is prepared by the following method:

[0108] (1) Activation: Bacillus amyloliquefaciens GKT04 was inoculated in NA medium, and cultured at 29°C for 23 hours;

[0109] Among them, the formula of LA medium is: tryptone 10g, yeast extract 5g, NaCl 10g, agar powder 12.5g, pH 7.0, sterilized at 121°C for 20min;

[0110] The formula of NA medium is: beef extract 3g, peptone 10g, NaCl 5g, glucose 4g, agar powder 12.5g, pH7.0, sterilized at 121℃ for 20min;

[0111] (2) Seed culture: inoculate the activated Bacillus amyloliquefaciens GKT04 strain into NA liquid medium, vibrate at 28°C and 150r / min, and cultivate for 8 hours to obtain a seed culture solution;

[0112] (3) Fermentation: Inoculate the seed culture liquid with 5% inoculation ratio in a mixture containing starch 10g / L, soybean meal powder 15g / L, glucose 5g / L, yeast extract 1g / L, L-sodium glutamate 3g / L L, sodium nitrate 1g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate 1g / ...

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Abstract

The invention relates to the technical field of biology, and particularly provides a Bacillus amyloliquefaciens strain named Bacillus amyloliquefaciens GKT04 (CCTCC NO: M2016784), a screening method of the Bacillus amyloliquefaciens GKT04, application of the Bacillus amyloliquefaciens GKT04 in inhibiting Banana vascular wilt, and a Bacillus amyloliquefaciens GKT04 microbial inoculant. The microbial inoculant has favorable preventing and treating effects on Banana vascular wilt, has long-time preventing actions, and is environment-friendly. Compared with other soil biocontrol bacteria, the strain can be stably colonized in the banana plant, is slightly influenced by the external environment, and can directly interact with pathogenic bacteria, thereby providing comprehensive and effective protection for plants. The experimental investigation detects that the strain can obviously promote growth of the banana plants.

Description

[0001] 【Technical field】 [0002] The technical field of microorganism application of the present invention specifically discloses a Bacillus amyloliquefaciens and its application. [0003] 【Background technique】 [0004] Bacillus (Bacillus spp.) is more widely used in plant disease biocontrol bacteria. It is an important class of microbial resources because it can produce spores resistant to heat, drought, ultraviolet rays, electromagnetic radiation and organic solvents, and can tolerate various adverse environmental conditions. Field application has proved that Bacillus biocontrol agents are significantly superior to non-Bacillus and fungal biocontrol agents in terms of stability, compatibility with chemical pesticides, and consistency of control effects on different plants in different years. And the bacillus can be made into powder and liquid. The powder is easy to store and transport, the large-scale production process is simple, and the cost is low. Therefore, Bacillus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 田丹丹黄素梅韦莉萍韦弟周维李朝生韦绍龙覃柳燕
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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